Degradation characteristics and kinetic analysis of phenolic pollutants by Rhodococcus biphenylivorans B403
XIE Xiaohang1,2,, YE Meng1,2, PAN Hong1,2, XU Songsiheng1, YANG Hui1, KE Yujie1, SONG Huiting1,2,, 1.Hubei Province Key Laboratory of Regional Develepment and Environment Response, School of Resources and Environment, Hubei University, Wuhan 430062, China 2.State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China
Abstract:The removal of phenolic pollutants is a key issue in the treatment of industrial wastewater, therefore, characterizing strains with degradation ability of phenolic pollutants is of great significance. In this study, the minimum inhibitory concentrations of Rhodococcus biphenylivorans B403 agaisnt six phenolic pollutants of phenol, such as m-cresol, catechol, p-nitrophenol, 2,4-dichlorophenol and 2,4,6-trichlorophenol, were determined. The results showed that the minimum inhibitory concentrations of strain B403 on phenol, m-cresol, catechol, p-nitrophenol, 2,4-dichlorophenol and 2,4,6-trichlorophenol were 1 190, 630, 700, 140, 70, 48 mg·L?1, respectively. And strain B403 had stronger tolerability on phenol, m-cresol and catechol than others. Subsequently, the growth and degradation properties of Rhodococcus biphenylivorans B403 for these three pollutants under different carbon sources were investigated. The strain B403 could effectively degrade phenol and m-cresol when a phenolic compound was used as the sole carbon source. After 30 h treatment in inorganic salt medium, the degradation rates of phenol, m-cresol and catechol were 97.85%, 100%, 56.54%, respectively. At the present of other organic carbon sources, the biomass of strain B403 greatly increased, and the degradation effieciency of the three types of phenolic pollutants was significantly improved. After 15 h treatment in the mixed medium of LB-inorganic salt, the degradation rates of phenol, m-cresol and catecholwere 98.92%, 99.93%, 94.35%, respectively. To further characterize the properties of strain B403, the Haldane model was used to fit the phenol degradation kinetics under optimal degradation conditions. For phenol degradation by strain B403 at different concentrations, the qm, Ks and KI were 0.503 h?1, 270.9 mg·L?1 and 69 mg·L?1, respectively. As to m-cresol and catechol degradation, the qm, Ks, KI were 0.672 h?1 and 1.749 h?1, 171.9 mg·L?1 and 541.9 mg·L?1, 23.74 mg·L?1 and 42.61 mg·L?1, respectively. Based on the kinetic equations, the optimum concentrations for phenol, m-cresol and catechol degradation by strain B403 were 136.4, 87.4, 116.1 mg·L?1, respectively. This study indicated that B403 presented stronger degradation ability agaisnt phenolic contaminants at the presence of other organic carbon sources,and strain B403 possesses certain application potential in the field of organic wastewater treatment with phenolic pollutants and organic phenol-contaminated soil remediation. Key words:biodegradation/ phenolic pollutants/ Rhodococcus biphenylivorans/ minimum inhibitory concentration/ degradation kinetics.
图1菌株B403以苯酚为底物时在3种培养基中的生长情况 Figure1.Growth of strain B403 in three mediumswith phenol as substrate
下载: 导出CSV 表2由Haldane模型得到的菌株B403降解苯酚、间甲酚、邻苯二酚的动力学参数 Table2.Degradation kinetic parameters of phenol, m-cresol and catechol by strain B403 obtained from Haldane model
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1.Hubei Province Key Laboratory of Regional Develepment and Environment Response, School of Resources and Environment, Hubei University, Wuhan 430062, China 2.State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China Received Date: 2019-08-19 Accepted Date: 2019-11-01 Available Online: 2020-05-06 Keywords:biodegradation/ phenolic pollutants/ Rhodococcus biphenylivorans/ minimum inhibitory concentration/ degradation kinetics Abstract:The removal of phenolic pollutants is a key issue in the treatment of industrial wastewater, therefore, characterizing strains with degradation ability of phenolic pollutants is of great significance. In this study, the minimum inhibitory concentrations of Rhodococcus biphenylivorans B403 agaisnt six phenolic pollutants of phenol, such as m-cresol, catechol, p-nitrophenol, 2,4-dichlorophenol and 2,4,6-trichlorophenol, were determined. The results showed that the minimum inhibitory concentrations of strain B403 on phenol, m-cresol, catechol, p-nitrophenol, 2,4-dichlorophenol and 2,4,6-trichlorophenol were 1 190, 630, 700, 140, 70, 48 mg·L?1, respectively. And strain B403 had stronger tolerability on phenol, m-cresol and catechol than others. Subsequently, the growth and degradation properties of Rhodococcus biphenylivorans B403 for these three pollutants under different carbon sources were investigated. The strain B403 could effectively degrade phenol and m-cresol when a phenolic compound was used as the sole carbon source. After 30 h treatment in inorganic salt medium, the degradation rates of phenol, m-cresol and catechol were 97.85%, 100%, 56.54%, respectively. At the present of other organic carbon sources, the biomass of strain B403 greatly increased, and the degradation effieciency of the three types of phenolic pollutants was significantly improved. After 15 h treatment in the mixed medium of LB-inorganic salt, the degradation rates of phenol, m-cresol and catecholwere 98.92%, 99.93%, 94.35%, respectively. To further characterize the properties of strain B403, the Haldane model was used to fit the phenol degradation kinetics under optimal degradation conditions. For phenol degradation by strain B403 at different concentrations, the qm, Ks and KI were 0.503 h?1, 270.9 mg·L?1 and 69 mg·L?1, respectively. As to m-cresol and catechol degradation, the qm, Ks, KI were 0.672 h?1 and 1.749 h?1, 171.9 mg·L?1 and 541.9 mg·L?1, 23.74 mg·L?1 and 42.61 mg·L?1, respectively. Based on the kinetic equations, the optimum concentrations for phenol, m-cresol and catechol degradation by strain B403 were 136.4, 87.4, 116.1 mg·L?1, respectively. This study indicated that B403 presented stronger degradation ability agaisnt phenolic contaminants at the presence of other organic carbon sources,and strain B403 possesses certain application potential in the field of organic wastewater treatment with phenolic pollutants and organic phenol-contaminated soil remediation.