赵小强,,
李文丽
甘肃农业大学甘肃省干旱生境作物学重点实验室 兰州 730070
基金项目: 甘肃农业大学甘肃省干旱生境作物学重点实验室开放基金GSCS-2019-8
甘肃农业大学甘肃省干旱生境作物学重点实验室开放基金GSCS-2020-5
甘肃农业大学科技创新基金-公招博士科研启动基金GAU-KYQD-2018-19
甘肃农业大学科技创新基金-公招博士科研启动基金GAU-KYQD-2018-12
甘肃省高等学校创新能力提升项目2019A-052
甘肃省高等学校创新能力提升项目2019A-054
国家自然科学基金项目32060486
兰州青绿仪器技术有限公司横向项目WT20191025
详细信息
作者简介:钟源, 主要从事生物化学与分子生物学研究。E-mail:zhongy@gsau.edu.cn
通讯作者:赵小强, 主要研究方向为现代生物技术及其在作物遗传育种中的应用。E-mail:zhaoxq3324@163.com
中图分类号:S513.035.2计量
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被引次数:0
出版历程
收稿日期:2020-06-27
录用日期:2020-08-19
刊出日期:2020-11-01
Association analysis of SSR markers with leaf morphology in maize (Zea mays) under diverse watering regimes
ZHONG Yuan,ZHAO Xiaoqiang,,
LI Wenli
Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China
Funds: the Open Fund of Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University,ChinaGSCS-2019-8
the Open Fund of Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University,ChinaGSCS-2020-5
the Scientific Research Start-up Funds for Openly-recruited Doctors of Science and Technology Innovation Funds of Gansu Agricultural University, ChinaGAU-KYQD-2018-19
the Scientific Research Start-up Funds for Openly-recruited Doctors of Science and Technology Innovation Funds of Gansu Agricultural University, ChinaGAU-KYQD-2018-12
the Developmental Funds of Innovation Capacity in Higher Education of Gansu Province, China2019A-052
the Developmental Funds of Innovation Capacity in Higher Education of Gansu Province, China2019A-054
National Natural Science Foundation of China32060486
the Transverse Project of Lanzhou Qingli Instrument and Technology Company, ChinaWT20191025
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Corresponding author:ZHAO Xiaoqiang, E-mail:zhaoxq3324@163.com
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摘要
摘要:玉米的叶形结构与其抗旱性紧密相关，挖掘不同水分环境下调控玉米叶形相关性状的显著关联分子标记位点，可为揭示玉米叶形结构的分子遗传机理、克隆相关调控基因并进行抗旱理想株型育种提供参考。本研究在不同水分环境下分析187份玉米自交系叶长、叶宽、叶夹角、叶向值、叶面积、叶形系数及叶片卷曲度等7个叶形相关性状的变化，采用SSR标记对这些材料进行全基因组扫描并分析其遗传多样性，采用一般线性模型（GLM）寻找不同水分环境下与玉米7个叶形相关性状关联的分子标记。结果表明：1）干旱环境下187份玉米自交系的叶长、叶宽、叶夹角、叶面积均显著降低，而叶向值、叶形系数及叶片卷曲度均明显升高，且干旱环境下这7个叶形相关性状的变异比率为30.53%~198.31%。2）145对SSR标记共检测出652个等位变异，变异范围在2~13个；多态性信息量（PIC）变异范围为0.201~0.966，平均0.478。采用UPGMA聚类及群体遗传结构分析均将供试材料分为旅大红骨（LRC）、唐四平头（TSPT）、兰卡斯特（Lan）、P及瑞德（Reid）等5大类群。3）采用GLM模型，在不同水分环境下共检测到15个SSR标记与玉米的7个叶形相关性状在P < 0.01水平下关联，各标记对表型的解释率为2.25%~27.30%，72.97%的标记可在干旱环境下被检测到；其中umc1124、umc2363、umc2214、umc1742、phi331888、umc1378、bnlg1863、umc2134和umc1345标记同时与不同水分环境下的多个叶形相关性状连锁，表现出明显的“一因多效”现象。这些研究结果将为玉米叶形结构的遗传改良及抗旱理想株型分子标记辅助选择育种提供参考。
关键词:玉米/
叶形结构/
干旱/
遗传结构/
关联分析
Abstract:The leaf morphology of maize (Zea mays) is closely related to its drought resistance. The molecular markers correlated with leaf morphology traits under different watering regimes may provide insights into the genetic mechanisms and identify the breeding varieties. In this study, changes in the leaf length (LL), leaf width (LW), leaf angle (LA), leaf orientation value (LOV), leaf size (LS), leaf shape coefficient (LSC), and leaf rolling index (LRI) of 187 maize inbred lines were analyzed under different watering regimes. A genome-wide scan and the genetic diversity were analyzed with simple-sequence repeat (SSR) markers, and the associations between the markers and the leaf morphological traits were assessed via a general linear model (GLM). The results showed that:1) the coefficients of variation for the leaf morphological traits of the 187 inbred lines were 13.21%-59.23% and 9.62%-28.22% under normal water and dry conditions, respectively, indicating abundant genetic variation. There were significant differences in the seven leaf morphological traits, so the leaf morphology was adjusted by the hereditary character of the inbred line. LL, LW, LA, and LS decreased significantly under drought stress, whereas LOV, LSC, and LRI increased, and the variance rate of the seven traits was 30.53%-198.31%. 2) A total of 652 alleles were detected using 145 SSR markers. The polymorphism information content (PIC) ranged from 0.201 to 0.966, with an average of 0.478. The tested materials were divided into five groups based on unweighted pair group method with arithmetic mean clustering and population structure; the Luda red cob group, Tangsipingtou group, Lancaster group, P group, and Reid group. 3) A total of 15 SSR markers were associated with seven leaf morphologies under different watering regimes (GLM, P < 0.01), and the amount of explained phenotypic variance was 2.25%-27.30%. Approximately 72.97% of the SSR markers were detected under drought conditions. The umc1124, umc2363, umc1742, phi331888, umc1378, bnlg1863, umc2134, and umc1345 markers were simultaneously associated with multiple leaf morphologies under different watering regimes, indicating pleiotropy. These results provide useful information for the genetic improvement of leaf morphology and marker-assisted selection breeding programs for maize drought resistance and optimization.
Key words:Maize/
Leaf morphology/
Drought/
Genetic structure/
Association analysis
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图1187份玉米自交系的群体遗传结构分析的 ΔK值随K值变化曲线
K: 群体个数 The number of population.
Figure1.Change curve in the log probability of data of ΔK value with K value of 187 maize inbred lines
下载: 全尺寸图片幻灯片
图2基于SSR标记的187份玉米自交系群体遗传结构
LRC:旅大红骨类群; TSPT:唐四平头类群; Lan:兰卡斯特类群; P: P群; Reid:瑞德类群; 不同颜色代表不同类群, 颜色多少表示该类群的概率。
Figure2.Population genetic structure of 187 maize inbred lines based on SSR
LRC: luda red cob group; TSPT: tangsipingtou group; Lan: lancaster group; P: P group; Reid: Reid group; Different colors indicate the corresponding groups, areas of colors indicate probability of the corresponding groups.
下载: 全尺寸图片幻灯片表1不同水分环境下187份玉米自交系叶形相关性状的表型值
Table1.Phenotypic values of leaf morphology traits of 187 maize inbred lines under different water environments
| 项目 Item | LL (cm) | LW (cm) | LA (°) | LOV | LS (cm2) | LSC | LRI | |||||||||||||
| CK | DS | CK | DS | CK | DS | CK | DS | CK | DS | CK | DS | CK | DS | |||||||
| 均值 Mean | 76.47±13.99 | 71.46±8.64 | 10.02±1.32 | 9.30±1.44 | 24.63±7.67 | 22.82±6.44 | 58.62±10.93 | 61.16±9.90 | 566.64±109.62 | 521.45±110.16 | 7.32±1.20 | 7.77±2.03 | 3.37±1.99 | 4.45±1.69 | ||||||
| 最大值 Max | 119.80 | 89.90 | 14.53 | 14.23 | 53.92 | 45.62 | 80.17 | 86.08 | 929.33 | 872.09 | 11.82 | 18.58 | 16.16 | 9.35 | ||||||
| 最小值 Min | 50.25 | 45.70 | 6.30 | 4.65 | 9.83 | 9.75 | 25.24 | 36.89 | 321.84 | 282.41 | 5.09 | 4.03 | 0.25 | 0.97 | ||||||
| 变异系数 Coefficient of variation (%) | 18.29 | 9.62 | 13.21 | 10.10 | 14.23 | 28.22 | 13.64 | 16.18 | 19.35 | 12.63 | 16.45 | 10.95 | 59.23 | 18.08 | ||||||
| 变异比率 Variation rate (%) | 52.60 | 76.46 | 198.31 | 118.62 | 65.27 | 66.57 | 30.53 | |||||||||||||
| 偏度 Skewness | 0.988 | -0.272 | 0.244 | 0.637 | 0.752 | 0.465 | -0.617 | -0.305 | 0.789 | 0.656 | 0.512 | 0.777 | 0.957 | 0.558 | ||||||
| 峰度 Kurtosis | 0.960 | -0.396 | 0.297 | 1.196 | 1.079 | 0.148 | 0.199 | -0.264 | 0.865 | 0.691 | 0.213 | 0.498 | 1.327 | 0.340 | ||||||
| ??LL:叶长; LW:叶宽; LA:叶夹角; LOV:叶向值; LS:叶面积; LSC:叶形系数; LRI:叶片卷曲度; CK:正常供水处理; DS:干旱胁迫处理。LL: leaf length; LW: leaf width; LA: leaf angle; LOV: leaf orientation value; LS: leaf size; LSC: leaf shape coefficient; LRI: leaf rolling index; CK: normal water treatment; DS: drought stress treatment. | ||||||||||||||||||||
下载: 导出CSV表2不同水分环境下187份玉米自交系叶形相关性状的方差分析(F值)
Table2.Variance analysis of leaf morphology traits for 187 maize inbred lines under different water environments (F-value)
| 变异来源 Variance source | LL | LW | LA | LOV | LS | LSC | LRI |
| 自交系 Inbred lines (IL) | 139.614** | 74.797** | 2.151* | 5.665** | 75.249** | 61.585** | 2.174* |
| 水分处理 Water treatment (WT) | 19.724** | 49.655** | 3.636** | 13.363** | 85.186** | 84.509** | 4.797** |
| 自交系×水分处理 IL×WT | 42.516** | 116.712** | 3.265** | 5.587** | 70.788** | 56.397** | 2.115* |
| ??*或**分别代表P < 0.05或P < 0.01水平差异显著。LL:叶长; LW:叶宽; LA:叶夹角; LOV:叶向值; LS:叶面积; LSC:叶形系数; LRI:叶片卷曲度。* or ** indicate significant difference at P < 0.05 or P < 0.01 levels, respectively. LL: leaf length; LW: leaf width; LA: leaf angle; LOV: leaf orientation value; LS: leaf size; LSC: leaf shape coefficient; LRI: leaf rolling index. | |||||||
下载: 导出CSV表3187份玉米自交系叶形相关性状的相关分析
Table3.Correlation analysis among leaf morphology traits of 187 maize inbred lines
| 性状 Trait | LL | LW | LA | LOV | LS | LSC | LRI |
| LL | 1.000 | ||||||
| LW | 0.218** | 1.000 | |||||
| LA | -0.119* | -0.104* | 1.000 | ||||
| LOV | -0.117* | -0.114* | -0.695** | 1.000 | |||
| LS | 0.639** | 0.725** | -0.155* | -0.210** | 1.000 | ||
| LSC | 0.258** | -0.382** | -0.121* | -0.061 | -0.179* | 1.000 | |
| LRI | -0.113* | -0.233** | -0.016 | 0.071 | -0.169* | 0.121* | 1.000 |
| ??*或**分别代表P < 0.05或P < 0.01水平显著相关。LL:叶长; LW:叶宽; LA:叶夹角; LOV:叶向值; LS:叶面积; LSC:叶形系数; LRI:叶片卷曲度。* or ** indicate significant correlation at P < 0.05 or P < 0.01 levels, respectively. LL: leaf length; LW: leaf width; LA: leaf angle; LOV: leaf orientation value; LS: leaf size; LSC: leaf shape coefficient; LRI: leaf rolling index. | |||||||
下载: 导出CSV表4145对SSR标记在187份玉米自交系中检测到的等位基因数及多态性信息量(PIC)
Table4.Allele number and polymorphism information content value (PIC) for 145 SSRs found in 187 maize inbred lines
| 标记 Marker | Bin | 等位基因数 No. of allele | PIC | 重复序列 Repeat sequence |
| bnlg149 | 1.01 | 7 | 0.628 | — |
| bnlg1484 | 1.03 | 3 | 0.256 | AG(19) |
| umc1124 | 1.05 | 4 | 0.424 | TCCC |
| umc2025 | 1.05 | 2 | 0.311 | — |
| umc1278 | 1.07 | 6 | 0.966 | (CT)12 |
| mmc0041 | 1.08 | 9 | 0.922 | — |
| phi011 | 1.09 | 2 | 0.231 | AGC |
| phi064 | 1.11 | 6 | 0.575 | ATCC |
| phi96100 | 2.01 | 6 | 0.840 | ACCT |
| umc2363 | 2.02 | 4 | 0.511 | — |
| umc1555 | 2.03 | 6 | 0.669 | (TTCA)7 |
| phi083 | 2.04 | 8 | 0.286 | AGCT |
| bnlg1613 | 2.04 | 2 | 0.461 | AG(27) |
| nc133 | 2.05 | 9 | 0.405 | GTGTC |
| umc1890 | 2.07 | 5 | 0.559 | (AT)8 |
| bnlg1633 | 2.07 | 4 | 0.437 | AG(16) |
| phi127 | 2.08 | 7 | 0.704 | AGAC |
| bnlg1233 | 2.08 | 7 | 0.731 | AG(24) |
| bnlg1940 | 2.08 | 6 | 0.524 | AG(18) |
| umc2214 | 2.09 | 4 | 0.298 | (GCAT)6 |
| umc1736 | 2.09 | 3 | 0.312 | (GCAT)6 |
| umc1780 | 3.00 | 3 | 0.224 | (CAC)4 |
| umc1746 | 3.00 | 5 | 0.505 | (CAC)4 |
| umc2376 | 3.01 | 4 | 0.260 | (ACACAC)4 |
| phi374118 | 3.02 | 8 | 0.682 | ACC |
| phi029 | 3.04 | 7 | 0.736 | AG/AGCG*** |
| umc1742 | 3.04 | 6 | 0.330 | (CGC)4 |
| phi053 | 3.05 | 6 | 0.237 | ATAC |
| phi073 | 3.05 | 13 | 0.672 | AGC |
| umc1539 | 3.05~3.06 | 4 | 0.394 | (GGC)5 |
| phi102228 | 3.06 | 12 | 0.586 | AAGC |
| umc1404 | 3.07 | 2 | 0.260 | (CCG)4 |
| umc1399 | 3.07 | 8 | 0.569 | (CTAG)5 |
| phi046 | 3.08 | 8 | 0.651 | ACGC |
| umc1320 | 3.08 | 4 | 0.460 | (GAAC)4 |
| phi047 | 3.09 | 5 | 0.357 | ATC |
| bnlg1496 | 3.09 | 4 | 0.378 | AG(18) |
| umc2176 | 4.00 | 3 | 0.315 | (TGC)4 |
| umc2206 | 4.00 | 4 | 0.681 | (GTAC)4 |
| umc2061 | 4.00 | 6 | 0.450 | (CTG)8 |
| phi072 | 4.00~4.01 | 5 | 0.540 | AAAC |
| bnlg1126 | 4.03 | 2 | 0.531 | — |
| umc1662 | 4.05 | 4 | 0.318 | (AGCC)4 |
| umc1031 | 4.05 | 5 | 0.383 | (CT)6AT(CT)9 |
| bnlg1621a | 4.06 | 5 | 0.516 | AG(18) |
| bnlg1927 | 4.07 | 6 | 0.629 | — |
| umc2038 | 4.07 | 9 | 0.881 | (GAC)4 |
| bnlg2162 | 4.08 | 3 | 0.593 | AG(27) |
| bnlg589 | 4.10 | 5 | 0.487 | — |
| phi006 | 4.11 | 5 | 0.373 | CCT |
| phi076 | 4.11 | 3 | 0.371 | AGCGGG |
| nc130 | 5.00 | 6 | 0.201 | AGC |
| umc2291 | 5.00 | 4 | 0.421 | (CCT)5 |
| phi024 | 5.01 | 4 | 0.670 | CCT |
| umc2388 | 5.02~5.03 | 3 | 0.243 | — |
| phi113 | 5.03~5.04 | 4 | 0.327 | GTCT |
| phi109188 | 5.03 | 4 | 0.377 | AAAG |
| phi331888 | 5.04 | 5 | 0.581 | AAG |
| umc1155 | 5.05 | 7 | 0.576 | (AG)20 |
| phi087 | 5.06 | 3 | 0.215 | ACC |
| umc2216 | 5.06 | 8 | 0.788 | (TCTC)5 |
| umc1072 | 5.07 | 7 | 0.273 | (GGA)10 |
| umc1225 | 5.08 | 4 | 0.225 | (AG)6 |
| bnlg1597c | 5.08 | 6 | 0.775 | (TCA)4 |
| umc1153 | 5.09 | 3 | 0.244 | (TCA)4 |
| umc1143 | 6.00 | 5 | 0.471 | AAAAT |
| umc2208 | 6.00 | 3 | 0.297 | (AAG)5 |
| umc2311 | 6.00 | 6 | 0.482 | — |
| phi126 | 6.00 | 3 | 0.863 | AG |
| phi423796 | 6.01 | 5 | 0.484 | AGATG |
| umc1378 | 7.00 | 4 | 0.763 | (CGC)6 |
| umc1409 | 7.01 | 4 | 0.429 | (GCTC)4 |
| bnlg1200 | 7.02 | 4 | 0.410 | AG(24) |
| umc1450 | 7.03 | 8 | 0.771 | (AC)10 |
| umc1708 | 7.04 | 3 | 0.235 | (GGA)4 |
| umc2042 | 8.01 | 7 | 0.733 | (GCC)4 |
| bnlg1073 | 8.01 | 4 | 0.410 | AG(19) |
| umc1327 | 8.01 | 4 | 0.446 | (GCC)4 |
| bnlg1194 | 8.02 | 3 | 0.853 | AG(33) |
| umc1974 | 8.02 | 4 | 0.542 | — |
| bnlg2235 | 8.02 | 4 | 0.555 | AG(23) |
| bnlg1352 | 8.02 | 4 | 0.405 | AG(16) |
| umc1304 | 8.02 | 5 | 0.470 | (TCGA)4 |
| umc1360 | 8.02 | 4 | 0.490 | (ACA)4 |
| bnlg2289 | 8.02 | 4 | 0.335 | AG(27) |
| umc1530 | 8.03 | 4 | 0.663 | (GGC)5 |
| bnlg1863 | 8.03 | 3 | 0.373 | AG(15) |
| umc2075 | 8.03 | 2 | 0.280 | (AGCCAG)4 |
| bnlg162 | 8.05 | 2 | 0.269 | — |
| bnlg666 | 8.05 | 5 | 0.529 | — |
| bnlg1812 | 8.05 | 5 | 0.554 | AG(22) |
| bnlg2181 | 8.05 | 2 | 0.246 | AG(19) |
| umc2356 | 8.05 | 4 | 0.323 | (TCT)5 |
| umc2395 | 8.06 | 4 | 0.524 | (CGA)4 |
| umc1607 | 8.07 | 4 | 0.348 | (TGC)5 |
| bnlg1823 | 8.07 | 3 | 0.665 | AG(18) |
| umc2218 | 8.08 | 4 | 0.539 | (TC)6 |
| umc1005 | 8.08 | 4 | 0.807 | (GT)15 |
| Umc1933 | 8.08 | 3 | 0.482 | — |
| phi233376 | 8.09 | 4 | 0.372 | CCG |
| umc1638 | 8.09 | 4 | 0.448 | (CTCCGG)5 |
| umc1916 | 8.09 | 3 | 0.348 | (CT)8 |
| umc2573 | 9.00 | 4 | 0.772 | TA |
| bnlg1401 | 9.02 | 3 | 0.320 | — |
| umc1033 | 9.02 | 4 | 0.656 | GA)25 |
| umc2337 | 9.03 | 3 | 0.247 | — |
| umc1586 | 9.03 | 4 | 0.824 | (ATC)5 |
| bnlg1730 | 9.03 | 2 | 0.654 | AG(26) |
| umc1258 | 9.03 | 4 | 0.451 | — |
| bnlg469a | 9.03 | 5 | 0.705 | — |
| bnlg1209 | 9.04 | 3 | 0.215 | AG(12) |
| umc1492 | 9.04 | 4 | 0.401 | (GCT)4 |
| umc1120 | 9.04 | 3 | 0.318 | (GGCAT)5 |
| umc1107 | 9.04 | 5 | 0.235 | — |
| umc2134 | 9.05 | 3 | 0.335 | (TTC)6 |
| umc2346 | 9.06 | 4 | 0.440 | (TA)7 |
| dupssr29 | 9.07 | 3 | 0.570 | (GA)24 |
| umc2359 | 9.07 | 4 | 0.611 | (AAAAG)4 |
| bnlg1588 | 9.07 | 2 | 0.295 | AG(21) |
| bnlg619 | 9.07 | 5 | 0.465 | — |
| umc1104 | 9.07 | 4 | 0.376 | GAT |
| umc1982 | 9.08 | 3 | 0.289 | — |
| dupssr8 | 10.00 | 3 | 0.472 | (TA)3 (CA)17 |
| umc1319 | 10.01 | 4 | 0.464 | (ACC)5 |
| bnlg1451 | 10.01 | 4 | 0.733 | AG(34) |
| umc1337 | 10.02 | 3 | 0.264 | (TA)8 |
| phi052 | 10.02 | 4 | 0.446 | AAG |
| bnlg1655 | 10.03 | 4 | 0.572 | AG(21) |
| umc1345 | 10.03 | 3 | 0.565 | (GCC)4 |
| umc2016 | 10.03 | 3 | 0.496 | (ACAT)4 |
| umc1246 | 10.04 | 4 | 0.328 | (AAAT)5 |
| umc1678 | 10.04 | 2 | 0.273 | (TCG)6 |
| bnlg1074 | 10.05 | 2 | 0.342 | AG(14) |
| umc1506 | 10.05 | 6 | 0.784 | (AACA)4 |
| umc1402 | 10.05 | 6 | 0.867 | (GCCC)4 |
| umc2221 | 10.05~10.06 | 4 | 0.210 | (GAGA)4 |
| bnlg236 | 10.06 | 4 | 0.288 | — |
| umc1249 | 10.06 | 2 | 0.335 | (TG)6 |
| umc1038 | 10.07 | 6 | 0.579 | (CT)15 |
| umc1645 | 10.07 | 5 | 0.585 | (CT)10 |
| bnlg1839 | 10.07 | 3 | 0.578 | AG(24) |
| bnlg1450 | 10.07 | 6 | 0.388 | AG(34) |
| bnlg1185 | 10.07 | 3 | 0.307 | AG(24) |
| bnlg1677 | 10.07 | 5 | 0.516 | AG(34) |
| bnlg1877 | 10.07 | 4 | 0.200 | — |
| ??***为不确定碱基; —为缺失。*** means uncertain base; — means missing. | ||||
下载: 导出CSV表5187份玉米自交系UPGMA聚类分析
Table5.UPGMA clustering of 187 maize inbred lines
| 杂种优势类群 Heterotic group | 自交系 Inbred lines |
| 旅大红骨Luda red cob group | 丹340、综31、117、0201、DM、137、综3、鲁原92、543、Va35-2、WN11、CW、9046、ZX-745半、锋1870半、ZX-957马、ZX-316马、ZX-382马、ZX-321马、ZX-128马、ZX-385马、ZX-964马、玉1768硬、ZX-25半、ZX-741半 Dan340, Zong31, 117, 0201, DM, 137, Zong3, Luyuan92, 543, Va35-2, WN11, CW, 9046, ZX-745ban, Feng1870ban, ZX-957ma, ZX-316ma, ZX-382ma, ZX-321ma, ZX-128ma, ZX-385ma, ZX-964ma, Yu1768ying, ZX-25ban, ZX-741ban |
| 唐四平头Tangsipingtou group | 黄早4、昌7-2、廊黄、430、黄201、马802A、502优、N192、323、W64A、H21、R802、兆111、W182BW、K805、吉853、吉H3、锦330、锋1973硬、LT-151-4N、LT-80-1N、锋1956半、LT-117-2N、LT-28-1N、LT-199-4N、CT-101-4、LT-100-3N、J-D17、W-D10、锋1913硬、ZX-236马、LT-55-1N、LT-121-6N、LT-202-3N Huangzao4, Chang7-2, Langhuang, 430, Huang201, Ma802A, 502You, N192, 323, W64A, H21, R802, Zhao111, W182BW, K805, Ji853, JiH3, Jin330, Feng1973ying, LT-151-4N, LT-80-1N, Feng1956ban, LT-117-2N, LT-28-1N, LT-199-4N, CT-101-4, LT-100-3N, J-D17, W-D10, Feng1913ying, ZX-236ma, LT-55-1N, LT-121-6N, LT-202-3N |
| 兰卡斯特Lancaster group | Mo17、314、廊H、TS109、ND246、AR234、Va26、黄C、自330、01743-2、Mo12、Pa91、A654、PL16、玉1824半、CT-54-1、LT-227-4N、玉1790半、ZX-622半、LT-134-3W、LT-197-2W、LT-204-10N、LT-121-4S、J-D101、J-D51、ZX-45半、PL208、J-D190 Mo17, 314, LangH, TS109, ND246, AR234, Va26, HuangC, Zi330, 01743-2, Mo12, Pa91, A654, PL16, Yu1824ban, CT-54-1, LT-227-4N, Yu1790ban, ZX-622ban, LT-134-3W, LT-197-2W, LT-204-10N, LT-121-4S, J-D101, J-D51, ZX-45ban, PL208, J-D190 |
| P | 齐319、N87-1、沈136、A413、K12、8802A、OH43-2、T58、78599-1、P138、12、H105W、599、231、TS164、X-178、598、锋1999马、LT-117-2S、LT-126-1D、玉1753硬、LT-215-1N、玉1823半、玉1769硬、ZX-622半、W-D98、J-D32、J-D53、Q6122-4-2-3、PL207、ZX-965马、玉1805马、LT-185-5D、玉1765硬、玉1837半、ZX-970马、PL206、LT-129-W4 Qi319, N87-1, Shen136, A413, K12, 8802A, OH43-2, T58, 78599-1, P138, 12, H105W, 599, 231, TS164, X-178, 598, Feng1999ma, LT-117-2S, LT-126-1D, Yu1753ying, LT-215-1N, Yu1823ban, Yu1769ying, ZX-622ban, W-D98, J-D32, J-D53, Q6122-4-2-3, PL207, ZX-965ma, Yu1805ma, LT-185-5D, Yu1765ying, Yu1837ban, ZX-970ma, PL206, LT-129-W4 |
| 瑞德Reid group | B73、掖478、掖488、CV、TS141、TS161、TS168、TS163、B68、B37、K22、TS165、CM、Ca317、81162、8723、郑22、H84、TS66、郑58、65232、TS110、Va102、锋1864硬、J-D103、LT-57-4D、J-D111、LT-59-2N、LT-187-1N、LT-230-11N、LT-149-9N、LT-15-4D、LT-206-4D、LT-107-3S、LT-231-1N、LT-208-7D、LT-129-2S、LT-20-4W、LT-898-2D、LT-85-4N、LT-205-2N、LT-222-4D、LT-222-1N、LT-184-6D、LT-186-5N、W-D3、W-D14、玉1772半、LT-172-2N、W-D25、LT-182-1N、LT-64-3N、LT-72-1N、LT-100-2S、LT-142-4W、LT-140-1D、LT-90-1N、LT-15-2N、LT-18-4S、W-D5、W-D6、W-D16 B73, Ye478, Ye488, CV, TS141, TS161, TS168, TS163, B68, B37, K22, TS165, CM, Ca317, 81162, 8723, Zheng22, H84, TS66, Zheng58, 65232, TS110, Va102, Feng1864ying, J-D103, LT-57-4D, J-D111, LT-59-2N, LT-187-1N, LT-230-11N, LT-149-9N, LT-15-4D, LT-206-4D, LT-107-3S, LT-231-1N, LT-208-7D, LT-129-2S, LT-20-4W, LT-898-2D, LT-85-4N, LT-205-2N, LT-222-4D, LT-222-1N, LT-184-6D, LT-186-5N, W-D3, W-D14, Yu1772ban, LT-172-2N, W-D25, LT-182-1N, LT-64-3N, LT-72-1N, LT-100-2S, LT-142-4W, LT-140-1D, LT-90-1N, LT-15-2N, LT-18-4S, W-D5, W-D6, W-D16 |
| ??字体加粗的材料为相应杂种优势类群的标准测验种。The bolded materials are the standard test species for the corresponding heterosis groups. | |
下载: 导出CSV表6不同水分环境下与玉米叶形相关性状相关的SSR标记及其对表型变异的解释率(P< 0.01)
Table6.Significance and explained phenotypic variation of the SSR associated with leaf morphology in maize under different (P< 0.01)?
| 标记 SSR | Bin | LL (CK/DS) | LW (CK/DS) | LA (CK/DS) | LOV (CK/DS) | LS (CK/DS) | LSC (CK/DS) | LRI (CK/DS) |
| bnlg149 | 1.01 | 27.30/11.50 | ||||||
| umc1124 | 1.05 | 5.79/3.24 | 2.97/— | |||||
| umc2363 | 2.02 | 3.54/3.18 | 2.94/2.42 | 4.11/— | 3.09/2.66 | |||
| umc2214 | 2.09 | 3.41/3.00 | 2.88/2.65 | 2.47/2.51 | 2.42/2.45 | |||
| umc2376 | 3.01 | 2.84/3.02 | ||||||
| umc1742 | 3.04 | 2.91/— | 2.60/— | 2.55/— | ||||
| bnlg1621a | 4.06 | 12.31/19.25 | ||||||
| phi331888 | 5.04 | 2.66/2.39 | 2.62/2.70 | 2.74/2.71 | 3.07/2.50 | 4.52/— | 3.01/— | 2.49/— |
| umc1378 | 7.00 | 3.13/3.25 | 2.87/— | 4.15/4.20 | 3.75/4.01 | 2.53/2.62 | ||
| bnlg1863 | 8.03 | 22.17/15.40 | —/9.44 | |||||
| umc2356 | 8.05 | —/3.54 | ||||||
| umc2218 | 8.08 | 2.58/2.44 | ||||||
| umc2134 | 9.05 | 3.18/— | 4.15/7.86 | |||||
| bnlg1451 | 10.01 | 3.74/2.69 | ||||||
| umc1345 | 10.03 | 3.24/9.64 | 27.41/2.25 | |||||
| ??LL:叶长; LW:叶宽; LA:叶夹角; LOV:叶向值; LS:叶面积; LSC:叶形系数; LRI:叶片卷曲度。CK:正常供水处理; DS:干旱胁迫处理。LL: leaf length; LW: leaf width; LA: leaf angle; LOV: leaf orientation value; LS: leaf size; LSC: leaf shape coefficient; LRI: leaf rolling index. CK: normal water treatment; DS: drought stress treatment. | ||||||||
下载: 导出CSV参考文献
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