王宇蕴^{1, 2,},
李兰²,
郑毅^{1, 2, 3},
汤利^{1, 2, 4,,}
1.云南农业大学植物保护学院 昆明 650201
2.云南农业大学资源与环境学院 昆明 650201
3.云南开放大学 昆明 650223
4.云南农业大学烟草学院 昆明 650201
基金项目: 国家重点研发计划项目2017YFD0200207
国家自然科学基金项目31760615
云南省农业基础研究联合专项项目2018FG001-104

详细信息

作者简介:王宇蕴, 主要研究方向为多样性种植体系根系-养分互作机制。E-mail:yuyunwhere@163.com

通讯作者:TANG Li, E-mail:ltang@ynau.edu.cn

中图分类号:S311

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被引次数:0

出版历程

收稿日期:2020-03-05
录用日期:2020-04-03
刊出日期:2020-07-01

Contribution of root morphology to phosphorus absorption in wheat and faba bean intercropping system

WANG Yuyun^{1, 2,},
LI Lan²,
ZHENG Yi^{1, 2, 3},
TANG Li^{1, 2, 4,,}
1. College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China
2. College of Resources and Environmental Sciences, Yunnan Agricultural University, Kunming 650201, China
3. Yunnan Open University, Kunming 650223, China
4. College of Tobacco, Yunnan Agricultural University, Kunming 650201, China
Funds: the National Key Research & Development Program of China2017YFD0200207
the National Natural Science Foundation of China31760615
the Joint Project of Basic Agriculture Research in Yunnan Province2018FG001-104



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摘要
摘要:间作模式是充分挖掘作物自身生物学潜力，促进磷资源高效利用的有效手段。为进一步探明间作诱导的根系形态适应性变化对磷吸收的相对贡献，理解小麦||蚕豆间作系统促进磷吸收的作用机制，本研究采用水培法，研究了常规磷（Hoagland营养液磷水平）和低磷（1/2-Hoagland营养液磷水平）条件下，小麦||蚕豆间作对小麦和蚕豆磷吸收、生长性状和根系形态变化的影响，并结合集成推进树算法（ABT）分析小麦和蚕豆根系形态对小麦和蚕豆磷吸收的相对贡献。结果表明，在低磷水平下，与单作小麦相比，小麦||蚕豆间作可以显著促进小麦对磷的吸收；而在正常磷水平下，间作小麦的磷吸收量显著低于单作小麦。在常规磷和低磷水平下，间作均能够显著促进蚕豆对磷的吸收。正常磷水平，间作系统的磷吸收量是小麦和蚕豆单作系统平均磷吸收量的1.04倍；低磷水平，间作系统的磷吸收量是小麦和蚕豆单作系统磷吸收量的1.28倍。种植模式和供磷水平能够显著影响小麦的株高，且有显著的交互作用；间作显著降低蚕豆的株高。ABT分析结果表明，小麦根长、根平均直径主导了小麦根系对磷的吸收，其解释量分别为74.7%和25.3%；蚕豆根长、根平均直径和根表面积共同解释了根系对磷的吸收，其解释量分别为48.0%、35.2%和16.9%。因此，低磷条件下，小麦||蚕豆间作主要通过增加小麦的根长，显著降低小麦根系平均直径，促进小麦对磷的吸收；而间作系统中的蚕豆则通过增加根长促进其对磷的吸收。
关键词:集成推进树算法(ABT)/
小麦||蚕豆间作/
根系形态/
磷素吸收
Abstract:Intercropping is an effective way of enhancing efficient use of phosphorus resources through using the biological potential of crops. This study aims to explore the relative contribution of root morphological adaptations to phosphorus absorption under intercropping, and to explore how the wheat||faba bean intercropping system promotes phosphorus uptake. Wheat and faba bean were grown hydroponically under either adequate or deficient phosphorus levels. The effect of intercropping on their phosphorus uptake, growth traits, and root morphology were analyzed. The relative contribution of root traits to phosphorus uptake by wheat and faba bean were then analyzed using the aggregated boosted trees (ABT) method. Our results showed that under phosphorus deficient conditions, the uptake of phosphorus by wheat was higher in the wheat||faba bean intercropping system than in the monoculture. Conversely, phosphorus uptake by wheat under adequate phosphorus conditions was lower in the intercropping system. The uptake of phosphorus by faba bean was higher in the intercropping system than in the monoculture under both phosphorus conditions. The intercropping system led to a total phosphorus uptake that was 1.04 and 1.28 times higher than that of the corresponding monocrop system under adequate and deficient phosphorus conditions, respectively. This result indicated an advantage of intercropping over monocropping. Crop patterns and phosphorus availability resulted in significant effect in wheat height; interaction between these two factors was also observed (P < 0.05). Intercropping led to a decrease in plant height for both species. The ABT analysis suggested that root length and diameter played a major role in phosphorus uptake by wheat, accounting for 74.4% and 25.3% uptake, respectively. Root length, diameter, and surface area all appeared to account for the uptake of phosphorus by faba bean, accounting for 48.0%, 35.2%, and 16.9% uptake, respectively. To conclude, under phosphorus deficient condition, the improved phosphorus uptake by wheat in wheat||faba bean intercropping system was the result of an increase in root length and a decrease in root diameter, while the phosphorus uptake by faba bean was improved owing to an increase in root length.
Key words:Aggregated Boosted Trees (ABT)/
Wheat||faba bean intercropping/
Root morphology/
Phosphorus uptake

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图1间作和供磷水平对小麦和蚕豆磷吸收的影响
P:正常供磷处理; 1/2 P:低磷处理。不同小写字母表示不同处理间差异显著(P < 0.05)。
Figure1.Effects of intercropping and phosphorus supply levels on phosphorus uptake of wheat and faba bean
P: Hoagland nutrient solution with normal phosphorus concentration; 1/2 P: Hoagland solution with 1/2-phosphorus concentration. Different lowercase letters indicate significant differences among treatments at P < 0.05 level.


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图2小麦和蚕豆根系形态指标对作物磷吸收的相对贡献
RL:根长; RD:根平均直径; RSA:根表面积。
Figure2.Relative influences of root morphological indicators on phosphorus uptake of wheat and faba bean
RL: root length; RD: average root diameter; RSA: root surface area.


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表1间作和不同供磷水平对小麦和蚕豆生长性状的影响
Table1.Effects of intercropping and phosphorus supply level on growth traits of wheat and faba bean

磷水平 P level	种植模式 Planting pattern	小麦 Wheat				蚕豆 Faba bean
		根系鲜重 Root weight (g)	根冠比 Root-shoot ratio	株高 Plant height (cm)		根系鲜重 Root weight (g)	根冠比 Root-shoot ratio	株高 Plant height (cm)
1/2 P	单作Monocropping	0.72±0.11b	0.49±0.06ab	31.00±1.71b		3.49±0.64a	0.37±0.02a	41.95±5.21a
	间作Intercropping	0.52±0.09c	0.52±0.05a	30.25±1.06b		3.87±0.57a	0.32±0.06a	34.10±4.31b
P	单作Monocropping	1.29±0.17a	0.55±0.11a	34.10±4.38ab		3.49±1.81a	0.34±0.02a	43.30±1.07a
	间作Intercropping	0.92±0.19b	0.46±0.16b	41.95±5.28a		3.87±0.21a	0.31±0.06a	35.50±1.69b
显著性Significance
供磷水平P level (T)		*	ns	**		ns	ns	ns
种植模式Planting pattern (M)		*	ns	*		ns	ns	**
T × M		**	ns	*		ns	ns	ns
P:正常供磷处理; 1/2 P:低磷处理。同列数据后不同字母表示不同处理间差异显著(P < 0.05)。*: P < 0.05; **: P < 0.01; ns:不显著。P: Hoagland nutrient solution with normal phosphorus concentration; 1/2 P: Hoagland solution with 1/2-phosphorus concentration. Different lowercase letters in the same column indicate significant differences among treatments at P < 0.05 level. *: P < 0.05; **: P < 0.01; ns: no significance.

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表2间作和供磷水平对小麦和蚕豆根系形态的影响
Table2.Effects of intercropping and phosphorus supply levels on root morphology of wheat and faba bean

磷水平 P level	种植模式 Planting pattern	小麦Wheat				蚕豆Faba bean
		根长 Root length (cm)	根平均直径 Average root diameter (mm)	根表面 Root surface area (cm2)		根长 Root length (cm)	根平均直径 Average root diameter (mm)	根表面 Root surface area (cm2)
1/2 P	单作Monocropping	252.51±4.11b	0.24±0.02a	18.33±0.27b		249.81±5.54d	0.47±0.09a	17.23±0.91ab
	间作Intercropping	268.35±3.23a	0.19±0.01b	20.39±0.59a		274.24±4.96c	0.48±0.02a	19.90±0.88a
P	单作Monocropping	270.54±2.32a	0.25±0.01a	19.87±0.36ab		294.87±5.06b	0.51±0.03a	20.62±0.66a
	间作Intercropping	273.00±2.21a	0.20±0.01b	20.40±0.68a		315.56±6.13a	0.51±0.05a	21.76±0.68a
显著性Significance
供磷水平P level (T)		*	ns	ns		*	ns	*
种植模式Planting pattern (M)		ns	*	ns		*	ns	ns
T × M		ns	ns	*		*	ns	ns
P:正常供磷处理; 1/2 P:低磷处理。同列数据后不同字母表示不同处理间差异显著(P < 0.05)。*: P < 0.05; **: P < 0.01; ns:不显著。P: Hoagland nutrient solution with normal phosphorus concentration; 1/2 P: Hoagland solution with 1/2-phosphorus concentration. Different lowercase letters in the same column indicate significant differences among treatments at P < 0.05 level. *: P < 0.05; **: P < 0.01; ns: no significance.

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