刘玲,
王苏杭,
张进,
陈成,
赵薪程,
刘海涛,
汪承润^,
淮南师范学院生物工程学院 淮南 232038
基金项目: 安徽省教育厅重点项目KJ2018A0472
安徽省重大专项项目18030701189
安徽省高校优秀青年人才支持计划gxyq2019078

详细信息

作者简介:刘玲, 主要研究方向为植物生态学及植物逆境生理。E-mail:lliiuu494@sina.com

通讯作者:汪承润, 主要研究方向为生态毒理学。E-mail:chengrunwang@163.com

中图分类号:X592

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被引次数:0

出版历程

收稿日期:2019-11-19
录用日期:2020-02-02
刊出日期:2020-05-01

Cd enrichment and distribution in broad bean seedlings under stress of Cd combined with MWCNTs-COOH

LIU Ling,
WANG Suhang,
ZHANG Jin,
CHEN Cheng,
ZHAO Xincheng,
LIU Haitao,
WANG Chengrun^,
School of Biological Engineering, Huainan Normal University, Huainan 232038, China
Funds: the Priority Projects of Education Department of Anhui ProvinceKJ2018A0472
the Major Special Projects of Anhui Province18030701189
the Excellent Young Talents Support Program for Colleges and Universities in Anhui Provincegxyq2019078

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Corresponding author:WANG Chengrun, E-mail:chengrunwang@163.com

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摘要
摘要:纳米材料因大量开发、生产和应用不可避免地被释放到环境中，给生态环境和人体健康带来潜在的风险。因此为了探究羧基化多壁碳纳米管（MWCNTs-COOH）和重金属Cd双重胁迫对植物器官中Cd的富集、转运及细胞中Cd分布的影响，以及为MWCNTs-COOH与Cd复合污染对植物的毒性和生态风险性评价提供理论依据，本研究以蚕豆幼苗为试验材料，采用水培方式，设置MWCNTs-COOH（0 mg·L^-1、1.5 mg·L^-1、3.0 mg·L^-1、6.0 mg·L^-1、12.0 mg·L^-1）+10.0 μmol·L^-1 Cd 5个处理组，用石墨炉原子吸收光谱法测定不同处理下蚕豆幼苗根茎叶及细胞中Cd的含量，分析MWCNTs-COOH复合Cd处理下蚕豆幼苗营养器官对Cd的富集、转运及细胞内分布状况。结果表明：复合胁迫下，3种营养器官Cd含量均高于对照；根茎叶对Cd的富集、Cd富集系数及器官间（根-茎、茎-叶）的转移系数均随MWCNTs-COOH浓度升高呈先升高后降低趋势，当MWCNTs-COOH浓度为6.0 mg·L^-1时，以上指标均达到最大值。同时，随着MWCNTs-COOH浓度的增大，根茎叶细胞中Cd逐渐从细胞壁向原生质体转移，加深了对细胞的毒害。综上所述，中低浓度的MWCNTs-COOH不仅可促进蚕豆根茎叶对Cd的累积及向上转运，而且也能加强细胞中Cd的转移。
关键词:羧基化多壁碳纳米管/
镉/
复合胁迫/
富集系数/
转移系数/
蚕豆
Abstract:Nanomaterials are inevitably released into the environment because of developed production and application, which brings potential risks to the ecological environment and human health. The aim of this study was to explore the effect of MWCNTs-COOH and heavy metals Cd double stress on Cd enrichment, transport, and distribution in plant organs, and provide a theoretical basis for evaluating phytotoxicity and ecological risk of the combined pollution of MWCNTs-COOH and Cd. In this study, broad bean seedlings were cultured as experimental material using the hydroponics method, and MWCNTs-COOH (0 mg·L^-1, 1.5 mg·L^-1, 3.0 mg·L^-1, 6.0 mg·L^-1, 12.0 mg·L^-1) and 10.0 μmol·L^-1 Cd treatment groups were set. The contents of Cd in roots, stems, leaves, and cells of broad bean seedlings under the different treatments were determined by Graphite Furnace atomic absorption spectrometry; and Cd enrichment, transport in vegetative organs, and distribution in cells of broad bean were analyzed. The results showed that Cd contents in three kinds of vegetative organs under MWCNTs-COOH and Cd compound stresses were higher than those in CK. Cd enrichment in roots, stems, and leaves; Cd enrichment coefficient, and translocation coefficient between organs (root-stem and stem-leaf) all first increased and then decreased. The above indicators reached their maximum values when the concentration of MWCNTs-COOH was 6.0 mg·L^-1. At the same time, with the increase of MWCNTs-COOH concentration, Cd in cells of roots, stems, and leaves gradually transferred from cell wall to protoplast, which deepened the toxicity to cells. In conclusion, medium and low concentrations of MWCNTs-COOH not only promoted Cd accumulation and upward transport in roots, stems, and leaves, but also enhanced Cd transfer in cells.
Key words:MWCNTs-COOH/
Cd/
Complex stress/
Enrichment coefficient/
Translocation coefficient/
Broad bean

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表1MWCNTs-COOH复合Cd胁迫下蚕豆幼苗各器官Cd含量
Table1.Contents of Cd in various organs of broad bean seedlings under stress of MWCNTs-COOH combined with Cd??μg·g^-1

处理Treatment	叶Leaf	茎Stem	根系Root
Cd (CK, 10.0 μmol·L-1Cd)	5.79±0.48e	17.45±0.31e	75.15±1.82c
Cd+1.5 mg·L-1 MWCNTs-COOH	7.84±0.39d	21.10±0.22d	86.10±1.82b
Cd+3.0 mg·L-1 MWCNTs-COOH	10.34±0.13c	24.87±0.34c	89.22±0.49ab
Cd+6.0 mg·L-1 MWCNTs-COOH	14.02±0.16a	30.33±0.33a	90.62±1.39a
Cd+12.0 mg·L-1 MWCNTs-COOH	11.78±0.27b	26.42±0.33b	85.66±1.06b
同列不同小写字母表示不同处理间在P < 0.05水平差异显著。Different lowercase letters in the same column indicate significant differences among different treatments at P < 0.05 level.

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表2MWCNTs-COOH复合Cd胁迫下蚕豆幼苗各器官对Cd的富集系数及转移系数
Table2.Bioconcentration factor and translocation factor of Cd in organs of broad bean seedlings under stress of MWCNTs-COOH combined with Cd

处理 Treatment	Cd富集系数 Cd bioconcentration factor (%)				Cd转移系数 Cd translocation factor (%)
	根 root	茎 Stem	叶 Leaf		根-茎 From root to shoot	茎-叶 From stem to leaf
Cd (CK, 10.0 μmol·L-1Cd)	5.58±0.13c	1.30±0.02e	0.43±0.04e		23.23±0.14e	33.16±2.16d
Cd+1.5 mg·L-1 MWCNTs-COOH	6.39±0.13b	1.57±0.02d	0.58±0.03d		24.51±0.26d	37.13±1.46c
Cd+3.0 mg·L-1 MWCNTs-COOH	6.62±0.04ab	1.85±0.02c	0.77±0.01c		27.87±0.23c	41.58±0.06b
Cd+6.0 mg·L-1 MWCNTs-COOH	6.72±0.11a	2.25±0.03a	1.04±0.01a		33.47±0.16a	46.23±0.02a
Cd+12.0 mg·L-1 MWCNTs-COOH	6.35±0.08b	1.96±0.03b	0.88±0.02b		30.84±0.01b	44.60±0.46ab
同列不同小写字母表示不同处理间在P < 0.05水平差异显著。Different lowercase letters in the same column indicate significant differences among different treatments at P < 0.05 level.

下载: 导出CSV
表3MWCNTs-COOH复合Cd胁迫下蚕豆根细胞亚显微结构Cd含量及分配比例
Table3.Cd contents and distribution ratios of submicrostructure in broad bean root cells under stress of MWCNTs-COOH combined with Cd

处理 Treatment	Cd含量 Cd content (μg·g-1)			分配比例 Distribution ratio (%)
	细胞壁 F1 Cell wall	原生质 F2 Protoplast		细胞壁 F1 Cell wall	原生质体 F2 Protoplast
Cd (CK, 10.0 μmol·L-1Cd)	7.51±0.33d	4.04±0.33e		67.03±3.64a	32.98±3.64d
Cd+1.5 mg·L-1 MWCNTs-COOH	8.68±0.04bc	6.41±0.25d		57.52±0.87b	42.49±0.87c
Cd+3.0 mg·L-1 MWCNTs-COOH	8.99±0.11ab	8.59±0.18c		51.14±0.23c	48.86±0.23b
Cd+6.0 mg·L-1 MWCNTs-COOH	9.28±0.06a	12.23±0.16a		43.14±0.16d	56.86±0.16a
Cd+12.0 mg·L-1 MWCNTs-COOH	8.42±0.13c	10.20±0.05b		45.23±0.25d	54.77±0.25a
同列不同小写字母表示不同处理间在P < 0.05水平差异显著。Different lowercase letters in the same column indicate significant differences among different treatments at P < 0.05 level.

下载: 导出CSV
表4MWCNTs-COOH复合Cd胁迫下蚕豆茎细胞亚显微结构Cd含量及分配比例
Table4.Cd contents and distribution ratios of submicrostructure in broad bean stem cells under stress of MWCNTs-COOH combined with Cd

处理 Treatment	Cd含量 Cd content (μg·g-1)			分配比例 Distribution ratio (%)
	细胞壁 F1 Cell wall	原生质 F2 Protoplast		细胞壁 F1 Cell wall	原生质体 F2 Protoplast
Cd (CK, 10.0 μmol·L-1Cd)	2.23±0.07d	2.14±0.10d		51.04±0.36a	48.97±0.36d
Cd+1.5 mg·L-1 MWCNTs-COOH	2.79±0.08c	3.23±0.23c		46.26±1.03b	53.74±1.03c
Cd+3.0 mg·L-1 MWCNTs-COOH	3.19±0.13b	4.02±0.07b		44.24±0.55c	55.76±0.55b
Cd+6.0 mg·L-1 MWCNTs-COOH	3.71±0.17a	4.80±0.08a		43.60±0.67bc	56.40±0.67bc
Cd+12.0 mg·L-1 MWCNTs-COOH	3.29±0.03b	4.46±0.12a		42.49±0.45d	57.52±0.45a
同列不同小写字母表示不同处理间在P < 0.05水平差异显著。Different lowercase letters in the same column indicate significant differences among different treatments at P < 0.05 level.

下载: 导出CSV
表5MWCNTs-COOH复合Cd胁迫下蚕豆叶细胞亚显微结构Cd含量及分配比例
Table5.Cd contents and distribution ratio of submicrostructure in broad bean leaf cells under stress of MWCNTs-COOH combined with Cd

处理 Treatment	Cd含量 Cd content (μg·g-1)			分配比例 Distribution ratio (%)
	细胞壁F1 Cell wall	原生质F2 Protoplast		细胞壁F1 Cell wall	原生质体F2 Protoplast
Cd (CK, 10.0 μmol·L-1Cd)	0.51±0.06e	0.45±0.02e		54.45±0.01a	46.66±1.58c
Cd+1.5 mg·L-1 MWCNTs-COOH	0.77±0.03d	0.78±0.01d		49.83±0.69b	50.17±0.69b
Cd+3.0 mg·L-1 MWCNTs-COOH	1.01±0.04c	1.13±0.05c		47.19±0.22c	52.82±0.22a
Cd+6.0 mg·L-1 MWCNTs-COOH	1.39±0.03a	1.67±0.08a		45.44±0.76d	54.57±0.76a
Cd+12.0 mg·L-1 MWCNTs-COOH	1.14±0.06b	1.33±0.06b		46.04±0.33cd	53.97±0.33a
同列不同小写字母表示不同处理间在P < 0.05水平差异显著。Different lowercase letters in the same column indicate significant differences among different treatments at P < 0.05 level.

下载: 导出CSV

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