不同春小麦品种耐低磷性评价及种质筛选

删除或更新信息，请邮件至freekaoyan#163.com(#换成@)

本站小编 Free考研考试/2022-01-01

刘露露^1,,
汪军成^{1, 2},
姚立蓉^{1, 2},
孟亚雄^{1, 2},
李葆春²,
杨轲¹,
司二静^{1, 2},
王化俊^{1, 2},
马小乐^{1, 2,,},
尚勋武¹,
李兴茂³
1.甘肃农业大学农学院兰州 730070
2.甘肃省干旱生境作物学重点实验室兰州 730070
3.甘肃省农业科学院旱作农业研究所兰州 730070
基金项目: 甘肃省农业小麦产业体系项目GARS-01-05
甘肃省教育厅高校科研项目2018D-14
甘肃省科技支撑计划项目1604NCK052
甘肃省科技重大专项计划项目17ZD2NA016
甘肃省农业科学院育种专项2019GAAS07

详细信息

作者简介:刘露露, 主要研究方向为小麦耐瘠薄特异种质的筛选。E-mail:755922374@qq.com

通讯作者:马小乐, 主要研究方向为小麦品质育种工作。E-mail:819166521@qq.com

中图分类号:S512.1+2

计量

文章访问数:272
HTML全文浏览量:7
PDF下载量:222
被引次数:0

出版历程

收稿日期:2020-02-18
录用日期:2020-03-20
刊出日期:2020-07-01

Evaluation of low phosphorus tolerance and germplasm screening of spring wheat

LIU Lulu^1,,
WANG Juncheng^{1, 2},
YAO Lirong^{1, 2},
MENG Yaxiong^{1, 2},
LI Baochun²,
YANG Ke¹,
SI Erjing^{1, 2},
WANG Huajun^{1, 2},
MA Xiaole^{1, 2,,},
SHANG Xunwu¹,
LI Xingmao³
1. College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China
2. Gansu Provincial Key Laboratory of Arid Land Crop Science, Lanzhou 730070, China
3. Dry Agriculture Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China
Funds: Gansu Agricultural Wheat Industry System ProjectGARS-01-05
the Science and Technology Project of Education Department of Gansu Province2018D-14
Gansu Provincial Science and Technology Support Plan Project1604NCK052
Gansu Provincial Science and Technology Major Special Project17ZD2NA016
the Breeding Special Project of Gansu Academy of Agricultural Sciences2019GAAS07

More Information

Corresponding author:MA Xiaole, E-mail:819166521@qq.com

摘要
HTML全文
图(5)表(5)
参考文献(28)
相关文章
施引文献
资源附件(0)
访问统计

摘要
摘要:筛选磷高效作物是充分利用土壤磷素和减少磷肥施用量的重要手段。本研究以162份春小麦种质资源为材料，对其苗期的株高、总根长、根表面积等8个指标的耐低磷系数进行分析，采用隶属函数法综合评价春小麦苗期的耐低磷特性，初步筛选耐低磷材料，并进一步进行成株期的耐低磷特性鉴定，筛选出耐低磷材料和磷敏感材料，分析其在低磷下酸性磷酸酶的活性变化。结果表明，低磷胁迫下春小麦材料苗期和成株期的各性状均受到不同程度的影响，并随着胁迫程度的增加，小麦生长受抑制程度增强。通过主成分分析将苗期8个指标转化成4个综合指标（累计贡献率为82.60%），将成株期的10个指标转化为3个综合指标（累计贡献率为83.23%）；采用隶属函数法计算耐低磷综合评价值（D）值，对D值进行聚类分析，将苗期的162份春小麦种质资源划分为耐低磷型（10份）、较耐低磷型（26份）、低磷较敏感型（91份）、低磷敏感型（35份）4类。选取耐低磷型材料（5份）和低磷敏感型材料（4份），进一步进行成株期鉴定，最终筛选1份耐低磷材料wp-35和1份磷敏感材料wp-119。通过分析其酸性磷酸酶活性，发现在低磷胁迫下春小麦根系和叶片中的酸性磷酸酶活性均升高，且耐低磷材料的酸性磷酸酶活性高于磷敏感材料。本研究结果可为解析春小麦耐低磷特性、培育耐低磷品种提供种质资源和理论依据。
Abstract:Phosphorus deficiency is one of the important abiotic stresses affecting agricultural production. Screening phosphorus-efficient crops is an important method for optimizing soil phosphorus and reducing phosphate fertilizer applications. In this study, the low phosphorus tolerance of 162 spring wheat germplasms was evaluated, and the tolerant materials were screened at seedling and adult stages. A correlation analysis and a principal component analysis of low phosphorus tolerance coefficients of eight indicators were conducted at the seedling stage to find the comprehensive indicators; a cluster analysis was performed to determine the low phosphorus tolerance of 162 materials according to their comprehensive evaluation value calculated with the subordinative function values. Then, low phosphorus tolerant materials and phosphorus sensitive materials were selected by determining the comprehensive evaluation value at the adult stage of the screened materials. The changes in acid phosphatase activity of the selected materials were analyzed. The results showed that there were different effects on the growth indicators at seedling stage and adult stage under low phosphorus stress, and that spring wheat growth inhibition increased as the stress time extension. Eight indicators at the seedling stage and 10 indicators at the adult stage were transformed into four comprehensive indicators (the cumulative contribution rate was 82.60%) and three comprehensive indicators (the cumulative contribution rate was 83.23%) by the principal component analysis, respectively. The cluster analysis showed that the 162 spring wheat germplasms at the seedling stage were divided into four types: low phosphorus tolerant (10 germplasms), slightly low phosphorus tolerant (26 germplasms), slightly low phosphorus sensitive (91 germplasms) and low phosphorus sensitive (35 germplasms). Five low phosphorus tolerant materials and four phosphorus sensitive materials were selected for phosphorus-efficient identification at the adult stage. Finally, one low phosphorus tolerant material, wp-35, and one phosphorus sensitive material, wp-119, were selected. The analysis of the two materials showed that the acid phosphatase activities in roots and leaves increased under low phosphorus stress, and that the acid phosphatase activity in wp-35 was higher than in wp-119. This study can provide germplasm resources, and a theoretical basis for the analysis of low phosphorus tolerance and the cultivation of low phosphorus tolerant spring wheat varieties.

HTML全文

图1耐低磷基因型与低磷敏感基因型小麦材料在成株期的长势情况
图A、B分别为磷高效利用材料wp-35和wp-139成熟期的表现, 图C、D分别为磷敏感材料wp-26和wp-119成熟期的表现。CK为正常磷水平处理, -P为低磷胁迫处理。
Figure1.Growth situation of wheat materials of low phosphorus tolerant and sensitive genotypes at maturity
A and B show the performance of efficient phosphorus utilization materials wp-35 and wp-139 at maturity. C and. D show the performance of phosphorus sensitive materials wp-26 and wp-119 at maturity, respectively. CK: normal phosphorus level; -P: low phosphorus level.

下载: 全尺寸图片幻灯片

图2基于种子袋筛选结果的春小麦材料成株期的耐低磷性聚类图
Figure2.Low phosphorus tolerance clustering diagram of spring wheat material during the whole growth period based on screening results

下载: 全尺寸图片幻灯片

图3春小麦耐低磷基因型wp-35和磷敏感基因型wp-119在不同磷水平处理下地上部(A)和根系(B)干重变化
CK:正常磷水平; -P:低磷胁迫。*代表对照与处理间差异达显著水平(P < 0.05)。
Figure3.Changes in dry weight of shoots (A) and roots (B) of spring wheat material wp-35 (low phosphorus tolerant) and wp-119 (low phosphorus sensitive) under different phosphorus levels
CK: normal phosphorus level; -P: low phosphorus level. * indicates significant differences between CK and-P treatments (P < 0.05).

下载: 全尺寸图片幻灯片

图4春小麦耐低磷基因型wp-35和磷敏感基因型wp-119在不同磷水平处理下根系(A)和叶片(B)APase活性变化
CK:正常磷水平; -P:低磷胁迫。*代表对照与处理间差异达显著水平(P < 0.05)。
Figure4.Changes of APase activity in roots (A) and leaves (B) of spring wheat material wp-35 (low phosphorus tolerant) and wp-119 (low phosphorus sensitive) under different phosphorus levels
CK: normal phosphorus level; -P: low phosphorus level. * indicates significant differences between CK and-P treatment (P < 0.05).

下载: 全尺寸图片幻灯片

图5春小麦耐低磷基因型wp-35和磷敏感基因型wp-119在不同磷水平处理下形态和经济性状的变化
CK:正常磷水平; -P:低磷胁迫。*代表对照与处理间差异达显著水平(P < 0.05)。
Figure5.Changes of morphology and economic traits of spring wheat material wp-35 (low phosphorus tolerant) and wp-119 (low phosphorus sensitive) under different phosphorus levels
CK: normal phosphorus level; -P: low phosphorus level. * indicates significant differences between CK and-P treatments (P < 0.05).

下载: 全尺寸图片幻灯片

表1供试春小麦材料苗期各生长性状耐低磷系数的相关性
Table1.Correlation among low phosphorus tolerance coefficients of growth indicators at seedling stage of the tested spring wheat materials

	株高 PH	根长 RL	总根长 TRL	根表面积 RSA	根体积 RV	地上部干重 ADW	地下部干重 UDW	根冠比 RSDW
株高PH	1.000
根长RL	0.070	1.000
总根长TRL	0.215^**	0.193^*	1.000
根表面积RSA	0.200^*	0.234^**	0.706^**	1.000
根体积RV	0.124	0.201^*	0.470^**	0.926^**	1.000
地上部干重ADW	0.211^**	0.184^*	0.512^**	0.540^**	0.476^**	1.000
地下部干重UDW	0.164^*	0.185^*	0.501^**	0.580^**	0.542^**	0.706^**	1.000
根冠比RSDW	-0.033	-0.015	-0.020	0.013	0.029	-0.281^**	0.300^**	1.000
和分别表示P < 0.05和P < 0.01显著相关。 and ** represents significant correlation at P < 0.05 and P < 0.01 levels, respectively. PH: plant height; RL: root length; TRL: total root length; RSA: root surface area; RV: root volume; ADW: aboveground dry weight; UDW: underground dry weight; RSDW: ratio of root and shoot dry weight.

下载: 导出CSV
表2供试春小麦材料苗期各生长指标耐低磷系数载荷矩阵、指标特征值及贡献率
Table2.Load matrix, characteristics value and contribution rate of low phosphorus tolerance coefficient of each growth indicator of the tested spring wheat materials at seedling stage

生长指标Growth indicator	主成分Principal component
生长指标Growth indicator	1	2	3	4
株高Plant height	0.301	-0.217	0.860	0.291
根长Root length	0.329	-0.062	-0.337	0.878
总根长Total root length	0.769	-0.056	0.041	-0.044
根表面积Root surface area	0.914	0.045	-0.113	-0.096
根体积Root volume	0.829	0.095	-0.189	-0.13
地上部干重Aboveground dry weight	0.772	-0.325	0.015	-0.129
地下部干重Underground dry weight	0.795	0.313	0.084	-0.062
根冠比Ratio of root and shoot	0.011	0.967	0.176	0.113
贡献率Contributive ratio (%)	44.30	15.06	11.78	11.46
权重Weight (%)	53.63	18.23	14.27	13.87

下载: 导出CSV
表3不同春小麦材料苗期的耐磷综合评价值(D)及耐低磷类型
Table3.Comprehensive evaluation value (D) and low phosphorus tolerance types of seedlings of different spring wheat materials

材料 Materials	D	排名 Order	类型 Type	材料 Materials	D	排名 Order	类型 Type	材料 Materials	D	排名 Order	类型 Type
wp-1	0.299 0	56	Ⅲ	wp-55	0.185 4	135	Ⅳ	wp-109	0.292 1	59	Ⅲ
wp-2	0.263 2	76	Ⅲ	wp-56	0.197 6	126	Ⅲ	wp-110	0.309 1	47	Ⅲ
wp-3	0.323 9	34	Ⅱ	wp-57	0.404 7	12	Ⅱ	wp-111	0.471 5	9	Ⅰ
wp-4	0.256 3	82	Ⅲ	wp-58	0.448 2	10	Ⅰ	wp-112	0.301 9	54	Ⅲ
wp-5	0.213 6	117	Ⅲ	wp-59	0.476 4	8	Ⅰ	wp-113	0.309 3	44	Ⅲ
wp-6	0.277 0	66	Ⅲ	wp-60	0.262 0	78	Ⅲ	wp-114	0.243 8	88	Ⅲ
wp-7	0.150 5	154	Ⅳ	wp-61	0.191 0	130	Ⅳ	wp-115	0.239 0	92	Ⅲ
wp-8	0.180 0	138	Ⅳ	wp-62	0.211 1	119	Ⅲ	wp-116	0.218 6	111	Ⅲ
wp-9	0.322 4	36	Ⅱ	wp-63	0.252 6	86	Ⅲ	wp-117	0.340 9	28	Ⅱ
wp-10	0.283 0	63	Ⅲ	wp-64	0.264 2	75	Ⅲ	wp-118	0.240 8	90	Ⅲ
wp-11	0.242 3	89	Ⅲ	wp-65	0.176 6	144	Ⅳ	wp-119	0.116 2	161	Ⅳ
wp-12	0.219 9	110	Ⅲ	wp-66	0.227 9	102	Ⅲ	wp-120	0.178 9	142	Ⅳ
wp-13	0.153 1	152	Ⅳ	wp-67	0.269 2	72	Ⅲ	wp-121	0.225 8	105	Ⅲ
wp-14	0.161 0	149	Ⅳ	wp-68	0.329 7	31	Ⅱ	wp-122	0.253 5	84	Ⅲ
wp-15	0.186 3	132	Ⅳ	wp-69	0.163 8	148	Ⅳ	wp-123	0.178 9	143	Ⅳ
wp-16	0.186 2	133	Ⅳ	wp-70	0.230 4	99	Ⅲ	wp-124	0.214 7	114	Ⅲ
wp-17	0.202 0	124	Ⅲ	wp-71	0.315 1	37	Ⅲ	wp-125	0.324 6	33	Ⅱ
wp-18	0.154 1	151	Ⅳ	wp-72	0.500 7	5	Ⅰ	wp-126	0.279 0	64	Ⅲ
wp-19	0.269 8	69	Ⅲ	wp-73	0.313 9	38	Ⅲ	wp-127	0.309 3	45	Ⅲ
wp-20	0.212 5	118	Ⅲ	wp-74	0.239 7	91	Ⅲ	wp-128	0.201 8	125	Ⅲ
wp-21	0.214 5	116	Ⅲ	wp-75	0.484 1	7	Ⅰ	wp-129	0.303 4	52	Ⅲ
wp-22	0.160 6	150	Ⅳ	wp-76	0.385 6	17	Ⅱ	wp-130	0.277 2	65	Ⅲ
wp-23	0.226 0	104	Ⅲ	wp-77	0.362 7	21	Ⅱ	wp-131	0.308 4	49	Ⅲ
wp-24	0.197 3	127	Ⅲ	wp-78	0.414 8	11	Ⅱ	wp-132	0.308 6	48	Ⅲ
wp-25	0.328 1	32	Ⅱ	wp-79	0.269 5	71	Ⅲ	wp-133	0.226 7	103	Ⅲ
wp-26	0.592 6	1	Ⅰ	wp-80	0.283 3	62	Ⅲ	wp-134	0.229 8	100	Ⅲ
wp-27	0.225 5	106	Ⅲ	wp-81	0.238 2	94	Ⅲ	wp-135	0.305 0	51	Ⅲ
wp-28	0.149 5	155	Ⅳ	wp-82	0.260 6	81	Ⅲ	wp-136	0.231 9	96	Ⅲ
wp-29	0.512 9	4	Ⅰ	wp-83	0.395 8	14	Ⅱ	wp-137	0.307 7	50	Ⅲ
wp-30	0.311 0	43	Ⅲ	wp-84	0.371 2	19	Ⅱ	wp-138	0.164 7	147	Ⅳ
wp-31	0.214 5	115	Ⅲ	wp-85	0.191 1	129	Ⅳ	wp-139	0.520 0	3	Ⅳ
wp-32	0.183 3	162	Ⅳ	wp-86	0.179 7	139	Ⅳ	wp-140	0.150 7	153	Ⅳ
wp-33	0.127 6	159	Ⅳ	wp-87	0.217 0	112	Ⅲ	wp-141	0.127 2	160	Ⅳ
wp-34	0.165 4	146	Ⅳ	wp-88	0.323 7	35	Ⅱ	wp-142	0.260 9	79	Ⅲ
wp-35	0.542 8	2	Ⅰ	wp-89	0.268 9	73	Ⅲ	wp-143	0.355 1	22	Ⅱ
wp-36	0.299 5	55	Ⅲ	wp-90	0.252 2	87	Ⅲ	wp-144	0.192 6	128	Ⅳ
wp-37	0.184 0	137	Ⅳ	wp-91	0.185 7	134	Ⅳ	wp-145	0.348 0	25	Ⅱ
wp-38	0.144 3	156	Ⅳ	wp-92	0.496 3	6	Ⅰ	wp-146	0.401 4	13	Ⅱ
wp-39	0.286 0	61	Ⅲ	wp-93	0.276 2	67	Ⅲ	wp-147	0.223 8	107	Ⅲ
wp-40	0.228 3	101	Ⅲ	wp-94	0.290 7	60	Ⅲ	wp-148	0.309 2	46	Ⅲ
wp-41	0.221 5	109	Ⅲ	wp-95	0.342 6	27	Ⅱ	wp-149	0.237 8	95	Ⅲ
wp-42	0.184 2	136	Ⅳ	wp-96	0.231 5	97	Ⅲ	wp-150	0.179 2	141	Ⅳ
wp-43	0.255 7	83	Ⅲ	wp-97	0.392 4	15	Ⅱ	wp-151	0.188 2	131	Ⅳ
wp-44	0.303 3	53	Ⅲ	wp-98	0.299 0	57	Ⅲ	wp-152	0.203 7	122	Ⅲ
wp-45	0.389 9	16	Ⅱ	wp-99	0.260 8	80	Ⅲ	wp-153	0.269 7	70	Ⅲ
wp-46	0.313 7	39	Ⅲ	wp-100	0.295 6	58	Ⅲ	wp-154	0.311 8	41	Ⅲ
wp-47	0.354 6	23	Ⅱ	wp-101	0.202 6	123	Ⅲ	wp-155	0.172 4	145	Ⅳ
wp-48	0.312 6	40	Ⅲ	wp-102	0.179 7	140	Ⅳ	wp-156	0.384 6	18	Ⅱ
wp-49	0.266 9	74	Ⅲ	wp-103	0.209 9	120	Ⅲ	wp-157	0.207 5	121	Ⅲ
wp-50	0.252 7	85	Ⅲ	wp-104	0.137 7	157	Ⅳ	wp-158	0.262 0	77	Ⅲ
wp-51	0.238 3	93	Ⅲ	wp-105	0.330 4	30	Ⅱ	wp-159	0.343 1	26	Ⅱ
wp-52	0.333 4	29	Ⅱ	wp-106	0.271 0	68	Ⅲ	wp-160	0.311 1	42	Ⅲ
wp-53	0.370 0	20	Ⅱ	wp-107	0.222 6	108	Ⅲ	wp-161	0.215 1	113	Ⅲ
wp-54	0.230 6	98	Ⅲ	wp-108	0.348 0	24	Ⅱ	wp-162	0.131 6	158	Ⅳ

下载: 导出CSV
表4供试春小麦材料成株期各综合指标的载荷矩阵、指标特征值及贡献率
Table4.Load matrix, index characteristic value and contribution rate of each comprehensive index at adult stage of spring wheat materials

耐低磷指数Low phosphorus tolerant index	主成分Principal component
耐低磷指数Low phosphorus tolerant index	1	2	3
总分蘖数Total number of tillers	0.327 5	0.766 8	-0.415 6
无效分蘖数Invalid tiller number	-0.754 2	-0.032 5	0.207 6
株高Plant height	0.904 4	-0.242 5	0.026 7
穗长Spike length	0.777 0	-0.398 3	0.418 6
基部不孕小穗Number of sterile spikelets at base	0.622 5	-0.337 7	0.494 9
穗粒数Grain number of spike	-0.586 7	0.372 5	0.642 4
单株粒数Grain number per plant	-0.354 6	0.812 1	0.404 2
地上生物量Biomass of shoot	0.735 6	0.647 4	0.150 8
株粒重Grain weight per plant	0.719 7	0.669 6	0.139 8
千粒重Thousand-grain weight	0.084 4	0.140 0	-0.663 8
贡献率Contributive ratio (%)	40.21	26.06	16.96
权重Weight	48.31	31.31	20.38

下载: 导出CSV
表5供试春小麦材料成熟期的综合性状指标值、权重、μ(X)及综合评价值(D)
Table5.Comprehensive index, index weight, μ(X) and comprehensive evaluation value (D) of the spring wheat materials at maturity stage

材料 Materials	Z1	Z2	Z3	μ(X₁)	μ(X₂)	μ(X₃)	综合评价值(D) Comprehensive assessment value (D)	排序Order
wp-26	-0.929 7	-0.656 1	-0.120 9	0.193 8	0.095 3	0.090 6	0.379 7	8
wp-29	0.280 8	1.000 5	-1.399 0	0.373 3	0.257 2	0.000 0	0.630 5	4
wp-32	-0.105 3	-1.630 2	1.078 3	0.316 1	0.000 0	0.175 6	0.491 6	6
wp-33	0.007 7	-0.985 9	-1.126 2	0.332 8	0.063 0	0.019 3	0.415 1	7
wp-35	0.799 9	-0.061 3	0.365 0	0.450 3	0.153 4	0.125 0	0.728 7	1
wp-72	0.996 4	0.110 1	-0.573 4	0.479 4	0.170 2	0.058 5	0.708 1	2
wp-119	-2.236 9	1.110 0	-0.150 1	0.000 0	0.267 9	0.088 5	0.356 5	9
wp-139	-0.336 4	0.010 5	1.474 9	0.281 8	0.160 4	0.203 7	0.645 9	3
wp-141	0.501 7	-0.469 6	-0.732 3	0.406 1	0.113 5	0.047 3	0.566 8	5
权重Weight	0.483 2	0.313 1	0.203 7

下载: 导出CSV

参考文献(28)

[1]	LAN P, LI W F, SCHMIDT W. 'Omics' approaches towards understanding plant phosphorus acquisition and use[M]//PLAXTON W C, LAMBERS H. Annual Plant Reviews. Hoboken, NJ: John Wiley & Sons, Inc., 2015, 48: 65-98
[2]	XU Y J, LIU F, HAN G M, et al. Genome-wide identification and comparative analysis of phosphate starvation-responsive transcription factors in maize and three other gramineous plants[J]. Plant Cell Reports, 2018, 37(5):711-726 doi: 10.1007/s00299-018-2262-0
[3]	朱春权, 朱晓芳, 沈仁芳.硫化氢促进缺磷条件下水稻根系细胞壁磷的再利用[J].土壤, 2018, 50(1):51-58 http://d.old.wanfangdata.com.cn/Periodical/tr201801007 ZHU C Q, ZHU X F, SHEN R F. Hydrogen sulfide promote rice (Oryza sativa) cell wall P remobilization under P starvation conditions[J]. Soils, 2018, 50(1):51-58 http://d.old.wanfangdata.com.cn/Periodical/tr201801007
[4]	丁广大, 陈水森, 石磊, 等.植物耐低磷胁迫的遗传调控机理研究进展[J].植物营养与肥料学报, 2013, 19(3):733-744 http://d.old.wanfangdata.com.cn/Periodical/zwyyyflxb201303025 DING G D, CHEN S S, SHI L, et al. Advances in genetic regulation mechanism of plant tolerance to phosphorus deficiency[J]. Journal of Plant Nutrition and Fertilizers, 2013, 19(3):733-744 http://d.old.wanfangdata.com.cn/Periodical/zwyyyflxb201303025
[5]	SCHR?DER J J, SMIT A L, CORDELL D, et al. Improved phosphorus use efficiency in agriculture:A key requirement for its sustainable use[J]. Chemosphere, 2011, 84(6):822-831 doi: 10.1016/j.chemosphere.2011.01.065
[6]	CORDELL D, DRANGERT J O, WHITE S. The story of phosphorus:Global food security and food for thought[J]. Global Environmental Change, 2009, 19(2):292-305 doi: 10.1016/j.gloenvcha.2008.10.009
[7]	BAKER A, CEASAR S A, PALMER A J, et al. Replace, reuse, recycle:Improving the sustainable use of phosphorus by plants[J]. Journal of Experimental Botany, 2015, 66(12):3523-3540 doi: 10.1093/jxb/erv210
[8]	宋爱梅, 黄新朋, 孙淑斌, 等.氮高效水稻品种苗期耐低磷种质的筛选与鉴定[J].中国水稻科学, 2010, 24(5):479-486 doi: 10.3969/j.issn.1001-7216.2010.05.006 SONG A M, HUANG X P, SUN S B, et al. Screening and identification of rice cultivars with relatively high nitrogen use efficiency for tolerance to phosphorus deficiency at seedling stage[J]. Chinese Journal of Rice Science, 2010, 24(5):479-486 doi: 10.3969/j.issn.1001-7216.2010.05.006
[9]	张吉海, 高世斌, 杨克诚, 等.玉米耐低磷种质资源的筛选与鉴定[J].植物遗传资源学报, 2008, 9(3):335-339 http://d.old.wanfangdata.com.cn/Periodical/zwyczyxb200803011 ZHANG J H, GAO S B, YANG K C, et al. Screening and identification for tolerance to low phosphorus stress of maize germplasm resources[J]. Journal of Plant Genetic Resources, 2008, 9(3):335-339 http://d.old.wanfangdata.com.cn/Periodical/zwyczyxb200803011
[10]	袁园园, 董贝, 曹晓慧, 等.黄淮麦区小麦成株期磷高效基因型的鉴定和筛选[J].麦类作物学报, 2017, 37(1):56-65 http://d.old.wanfangdata.com.cn/Periodical/mlzwxb201701008 YUAN Y Y, DONG B, CAO X H, et al. Identification and screening of high phosphorus use efficiency genotypes of wheat at adult stage in Huang-Huai wheat area[J]. Journal of Triticeae Crops, 2017, 37(1):56-65 http://d.old.wanfangdata.com.cn/Periodical/mlzwxb201701008
[11]	苑乂川, 陈小雨, 李明明, 等.谷子苗期耐低磷种质筛选及其根系保护酶系统对低磷胁迫的响应[J].作物学报, 2019, 45(4):601-612 http://d.old.wanfangdata.com.cn/Periodical/zuowxb201904011 YUAN Y C, CHEN X Y, LI M M, et al. Screening of germplasm tolerant to low phosphorus of seedling stage and response of root protective enzymes to low phosphorus in foxtail millet[J]. Acta Agronomica Sinica, 2019, 45(4):601-612 http://d.old.wanfangdata.com.cn/Periodical/zuowxb201904011
[12]	郭再华, 贺立源, 徐才国.磷水平对不同耐低磷水稻苗根系生长及氮、磷、钾吸收的影响[J].应用与环境生物学报, 2006, 12(4):449-452 doi: 10.3321/j.issn:1006-687X.2006.04.001 GUO Z H, HE L Y, XU C G. Effect of phosphorus level on root growth and N, P & K uptake of rice with different P efficiencies at seedling stage[J]. Chinese Journal of Applied & Environmental Biology, 2006, 12(4):449-452 doi: 10.3321/j.issn:1006-687X.2006.04.001
[13]	丁洪, 李生秀, 郭庆元, 等.酸性磷酸酶活性与大豆耐低磷能力的相关研究[J].植物营养与肥料学报, 1997, 3(2):123-128 doi: 10.3321/j.issn:1008-505X.1997.02.005 DING H, LI S X, GUO Q Y, et al. Study on correlation between acid phosphatase activity and low phosphorus tolerance of soybean[J]. Journal of Plant Nutrition and Fertilizers, 1997, 3(2):123-128 doi: 10.3321/j.issn:1008-505X.1997.02.005
[14]	张嘉莉, 朱从桦, 豆攀, 等.硅、磷配施对玉米苗期生长及氮磷钾积累的影响[J].中国生态农业学报, 2017, 25(5):677-688 http://www.ecoagri.ac.cn/zgstny/ch/reader/view_abstract.aspx?flag=1&file_no=20170506&journal_id=zgstny ZHANG J L, ZHU C H, DOU P, et al. Effect of phosphorus and silicon application on the uptake and utilization of nitrogen, phosphorus and potassium by maize seedlings[J]. Chinese Journal of Eco-Agriculture, 2017, 25(5):677-688 http://www.ecoagri.ac.cn/zgstny/ch/reader/view_abstract.aspx?flag=1&file_no=20170506&journal_id=zgstny
[15]	DA SILVA A, BRUNO I P, FRANZINI V I, et al. Phosphorus uptake efficiency, root morphology and architecture in Brazilian wheat cultivars[J]. Journal of Radioanalytical and Nuclear Chemistry, 2016, 307(2):1055-1063 doi: 10.1007/s10967-015-4282-3
[16]	YUAN Y Y, GAO M G, ZHANG M X, et al. QTL mapping for phosphorus efficiency and morphological traits at seedling and maturity stages in wheat[J]. Frontiers in Plant Science, 2017, 8:614 doi: 10.3389/fpls.2017.00614
[17]	REN P R, MA X L, LI B C, et al. Identification and selection of low-phosphate-tolerant germplasm in barley (Hordeum vulgare L.)[J]. Soil Science and Plant Nutrition, 2016, 62(5/6):471-480 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.1080/00380768.2016.1223521
[18]	WANG J, SUN J J, MIAO J, et al. A phosphate starvation response regulator Ta-PHR1 is involved in phosphate signalling and increases grain yield in wheat[J]. Annals of Botany, 2013, 111(6):1139-1153 doi: 10.1093/aob/mct080
[19]	LIU Y, MI G H, CHEN F J, et al. Rhizosphere effect and root growth of two maize (Zea mays L.) genotypes with contrasting P efficiency at low P availability[J]. Plant Science, 2004, 167(2):217-223 doi: 10.1016/j.plantsci.2004.02.026
[20]	连盈, 卢娟, 胡成梅, 等.低氮胁迫对谷子苗期性状的影响和耐低氮品种的筛选[J].中国生态农业学报(中英文), 2020, 28(4):523-534 http://www.ecoagri.ac.cn/zgstny/ch/reader/view_abstract.aspx?flag=1&file_no=2020-0406&journal_id=zgstny LIAN Y, LU J, HU C M, et al. Effects of low nitrogen stress on foxtail millet seedling characteristics and screening of low nitrogen tolerant varieties[J]. Chinese Journal of Eco-Agriculture, 2020, 28(4):523-534 http://www.ecoagri.ac.cn/zgstny/ch/reader/view_abstract.aspx?flag=1&file_no=2020-0406&journal_id=zgstny
[21]	LIAO M T, FILLERY I R P, PALTA J A. Early vigorous growth is a major factor influencing nitrogen uptake in wheat[J]. Functional Plant Biology, 2004, 31(2):121-129 doi: 10.1071/FP03060
[22]	王凡坤, 薛珂, 付为国.土壤氮磷状况对小麦叶片养分生态化学计量特征的影响[J].中国生态农业学报(中英文), 2019, 27(1):60-71 http://www.ecoagri.ac.cn/zgstny/ch/reader/view_abstract.aspx?flag=1&file_no=2019-0107&journal_id=zgstny WANG F K, XUE K, FU W G. Effects of soil nitrogen and phosphorus contents on ecological stoichiometry of wheat leaf[J]. Chinese Journal of Eco-Agriculture, 2019, 27(1):60-71 http://www.ecoagri.ac.cn/zgstny/ch/reader/view_abstract.aspx?flag=1&file_no=2019-0107&journal_id=zgstny
[23]	张恩和, 王惠珍, 阎秋洁.不同基因型春小麦对磷胁迫的适应性反应[J].麦类作物学报, 2006, 26(2):117-120 doi: 10.3969/j.issn.1009-1041.2006.02.025 ZHANG E H, WANG H Z, YAN Q J. Adaptable effects of phosphorus stress on different genotypes of spring wheat[J]. Journal of Triticeae Crops, 2006, 26(2):117-120 doi: 10.3969/j.issn.1009-1041.2006.02.025
[24]	杨瑞吉, 张小红, 王鹤龄, 等.不同基因型春小麦对磷胁迫适应性研究[J].西北植物学报, 2005, 25(11):2314-2318 doi: 10.3321/j.issn:1000-4025.2005.11.031 YANG R J, ZHANG X H, WANG H L, et al. Adaptabilities of different genotypes of spring wheat to phosphorous deficiency[J]. Acta Botanica Boreali-Occidentalia Sinica, 2005, 25(11):2314-2318 doi: 10.3321/j.issn:1000-4025.2005.11.031
[25]	LóPEZ-ARREDONDO D L, LEYVA-GONZáLEZ M A, GONZáLEZ-MORALES S I, et al. Phosphate nutrition:Improving low-phosphate tolerance in crops[J]. Annual Review of Plant Biology, 2014, 65(1):95-123 doi: 10.1146/annurev-arplant-050213-035949
[26]	孔忠新, 杨丽丽, 张政值, 等.小麦耐低磷基因型的筛选[J].麦类作物学报, 2010, 30(4):591-595 http://d.old.wanfangdata.com.cn/Periodical/mlzwxb201004001 KONG Z X, YANG L L, ZHANG Z Z, et al. Screening of wheat germplasms tolerant to low phosphorus[J]. Journal of Triticeae Crops, 2010, 30(4):591-595 http://d.old.wanfangdata.com.cn/Periodical/mlzwxb201004001
[27]	龚丝雨, 梁喜欢, 杨帅强, 等.低磷胁迫对不同磷效率基因型烟草苗期生长及生理特征的影响[J].核农学报, 2019, 33(6):1217-1224 http://d.old.wanfangdata.com.cn/Periodical/hnxb201906021 GONG S Y, LIANG X H, YANG S Q, et al. Effect on growth and physiological characteristics of tobacco genotypes with different P-efficiency at seedling stage under low-phosphorus stress[J]. Journal of Nuclear Agricultural Sciences, 2019, 33(6):1217-1224 http://d.old.wanfangdata.com.cn/Periodical/hnxb201906021
[28]	黄爱缨, 蔡一林, 滕中华, 等.玉米自交系苗期耐低磷的根系生理特性研究[J].中国生态农业学报, 2008, 16(6):1419-1422 http://www.ecoagri.ac.cn/zgstny/ch/reader/view_abstract.aspx?flag=1&file_no=2008615&journal_id=zgstny HUANG A Y, CAI Y L, TENG Z H, et al. Physiological characteristics of inbred line maize seedling root under phosphorus stress[J]. Chinese Journal of Eco-Agriculture, 2008, 16(6):1419-1422 http://www.ecoagri.ac.cn/zgstny/ch/reader/view_abstract.aspx?flag=1&file_no=2008615&journal_id=zgstny