关键词:芥菜型油菜; 多室角果; BjMc2; 分子标记 Fine Mapping of Polycyetic Gene ( Bjmc2) in Brassica juncea L. WANG Gang, ZHANG Xiang-Xiang, XU Ping, LYU Ze-Wen, WEN Jing, YI Bin, MA Chao-Zhi, TU Jin-Xing, FU Ting-Dong, SHEN Jin-Xiong* National Key Laboratory of Crop Genetic Improvement / National Research Center of Rapeseed Engineering and Technology, Huazhong Agricultural University, Wuhan 430070, China Fund:This study was supported by the National Science Foundation of China (31571698). AbstractMultilocular plants have a higher seed yield than bilocular plants in B. juncea, mapping and cloning the multilocular gene(s) might be helpful for rapeseed genetic improvement and explanation of the multilocular trait development mechanism. This study verified backcross populations derived from JD11-2 family different at BjMc2 locus only can be used for BjMc2 mapping. Using AFLP assay and BSA method in BC5 and BC6 generations, one AFLP marker linked to the target gene was obtained and converted to SCAR marker (SC1). On the basis of homologous sequences of the AFLP maker in B. rapa, 11 SSR markers and one SCAR marker were identified. Through the screening in the ZBjH BAC library of Brassica juncea, two BACs covered the target area were selected and one SSR marker was developed. All the developed SCAR and SSR markers were then used to detect the BC7 population, and a linkage map for the bilocular gene BjMc2 was built. ZX17 and BACsr96, the closest flanking markers, were mapped at 0.048 cM and 0.340 cM distant from the BjMc2 gene, respectively. Bjmc2 is positioned of 68 kb between 946 kb and 1014 kb in the Scaffold 000019 physical map of A7 in B. rapa. This result would lay a foundation for cloning polycyetic gene Bjmc2and selecting polycyetic lines by marker-assisted selection.
图2 与BjMc1连锁标记SR02在不同基因型单株中的扩增1~2:BjMc2位点定位群体中两室单株和多室单株; 3~4:BjMc1位点定位群体中两室单株和多室单株。Fig. 2 Amplification in diverse genotypes using marker SR02 linked toBjMc11-2: bilocular and polycyetic plants in population with BjMc2; 3-4: bilocular and polycyetic plants in population with BjMc1.
图5 芥菜型油菜两室基因BjMc2的遗传连锁图和白菜A7同源Scaffold物理图谱A是BjMc2的遗传连锁图; B是白菜A7同源Scaffold物理图谱, 黑色代表Scaffold 000101, 灰色代表Scaffold 000019。Fig. 5 Genetic linkage map of bilocular gene BjMc2 inB .junceand physical map of homologous Scaffolds of A7 in B. rapaA is the genetic linkage map of the bilocular gene BjMc2; B is physical map of homologous Scaffolds of A7 in B. rapa, black represent Scaffold 000101, gray represent Scaffold 000019.
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