关键词:烟草; 细胞质雄性不育; ATP合酶; 亚基基因; RNA编辑 Editing Sites in Transcript of Four F0-ATPase Subunit Gene in Tobacco TAO Yao1, WANG Yu1,3, ZHONG Si-Rong1, WU Lin-Min1, XIE Li-Juan1, NIE Ya-Ping1, ZHOU Wei2, WANG Jian-Ge4, LIU Qi-Yuan1,* 1 Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / Key Laboratory of Crop Physiology, Ecology and Genetic Breeding of Jiangxi Province / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
2 Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha 410128, China
3Agricultural committee of Guizhou province Qianxinanzhou, Xingyi 562400, China
4 College of Landscape Architecture and Art, Jiangxi Agricultural University, Nanchang 330045, China
Fund:This study was supported by the National Natural Science Foundation of China (31260350, 31301388), China Postdoctoral Science Foundation (2015T80870, 2014M562109), and Science and Technology Plan Projects of Jiangxi Province Education Department (GJJ13275). AbstractRNA editing exits extensively in mitochondria of higher plants and is one of the most important post-transcriptional regulation methods of gene expression in mitochondrial genomes of higher plants. At the same time, it is an essential process for forming function proteins. RNA editing can induce mutations in mitochondrial genes including nucleotide insertion, substitution, or deletion, which further affects the splicing and processing of primary transcripts, ultimately resulting in cytoplasmic male sterility (CMS). The results of research using multiple species showed that there is an obvious relationship between the four subunit genes of F0-ATPase and CMS. To explore the relationship, we studied RNA editing status of four mitochondrial genes atp6, atp9, orf25, and orfB from three tobacco male sterility lines (MS Zhongyan 90, MS Yunyan 85, MS K326) and their corresponding fertile lines . The four mitochondrial genes atp6, atp9, orf25, and orfB and their cDNA were distinctively amplified by PCR from six tobacco lines. After that, by means of making a comparison between the DNA sequences and the cDNA sequences of target genes to find RNA editing sites. The orf25and orfB gene transcripts had the same RNA editing sites between male sterile and fertile lines. For atp6 gene, RNA editing didn't occur in male sterile lines, while there were six RNA editing sites in fertile lines, which all caused changes in the type of amino acids and there were four editing sites enhancing hydrophobicity of the amino acids. It inferred that the difference of protein's hydrophobicity was most likely to cause CMS. The atp9 gene had ten RNA editing sites in fertile lines, eight of which were the same as those in male sterile lines, while two C→T unique editing sites were absent in male sterile lines, of which one caused changes in amino acid. The nucleotide variations at 223 site of atp9 gene resulted in producing a termination code, which might be the necessary RNA editing to produce normal functional protein. These results suggest that lacking of the unique RNA editing sites might contribute to CMS property in tobacco.
Keyword:Tobacco; Cytoplasmic male sterility; ATPase; Subunit gene; RNA editing Show Figures Show Figures
图5 不育系及其保持系烟草中orf25基因的RNA编辑位点及基因突变位点59、71、89、215、227、248、251、395、407、416位点为不育系和保持系orf25基因的RNA编辑位点; 9、165位点为不育系orf25基因的基因突变位点。Fig. 5 RNA editing sites and gene mutation sites oforf25 gene between cytoplasmic male sterility and maintainer lines in tobaccoRNA editing sites oforf25 gene between cytoplasmic male sterility and maintainer lines were sites 59, 71, 89, 215, 227, 248, 251, 395, 407, 416; Gene mutation sites oforf25 in cytoplasmic male sterility were sites 9, 165.
图6 不育系及其保持系烟草中orfB基因的RNA编辑位点及基因突变位点47、58、76、443位点为不育系和保持系orfB基因的RNA编辑位点; 363为不育系orfB基因的基因突变位点。Fig. 6 RNA editing sites and gene mutation sites oforfB gene between cytoplasmic male sterility and maintainer lines in tobaccoRNA editing sites oforfB gene between cytoplasmic male sterility and maintainer lines were sites 47, 58, 76, 443; Gene mutation sites oforfB in cytoplasmic male sterility were site 363.
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