姬拉拉,
王锐洁,
刘筱,
杨淑君,
关萍,
王健健,
贵州大学生命科学学院/贵州大学农业生物工程研究院/山地植物资源保护与种质创新教育部重点实验室/山地生态与农业生物工程协同创新中心 贵阳 550025
基金项目: 国家自然科学基金项目31760155
贵州省科技厅自然科学基金项目2016(1033)
贵州省生物学一流学科建设项目GNYL[2017]009
详细信息
作者简介:邓小红, 研究方向为植物生理生态。E-mail:2509077578@qq.com
通讯作者:王健健, 主要从事植物生理生态研究工作。E-mail:wangjj33209@163.com
中图分类号:S521计量
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被引次数:0
出版历程
收稿日期:2019-03-28
录用日期:2019-05-15
刊出日期:2019-08-01
Response of seed germination and physiological mechanism of Vigna angularis and Dolichos lablab to salt stress
DENG Xiaohong,JI Lala,
WANG Ruijie,
LIU Xiao,
YANG Shujun,
GUAN Ping,
WANG Jianjian,
College of Life Sciences, Institute of Agro-bioengineering, Guizhou University/Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education/Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering, Guiyang 550025, China
Funds: the National Natural Science Foundation of China31760155
the Natural Science Foundation of Guizhou2016(1033)
the Construction Program of Biology First-class Discipline in GuizhouGNYL[2017]009
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Corresponding author:WANG Jianjian, E-mail:wangjj33209@163.com
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摘要
摘要:为探讨红豆和白扁豆种子萌发及幼苗生长对盐胁迫的响应及其生理机制，以红豆品种‘渝红豆2号’和传统白扁豆品种为材料，分别用不同浓度NaCl（0 mmol·L-1、20 mmol·L-1、40 mmol·L-1、60 mmol·L-1、80 mmol·L-1、100 mmol·L-1）溶液处理种子，测定不同NaCl浓度胁迫下红豆和白扁豆种子的发芽指标及幼苗生长指标、叶片丙二醛（MDA）含量、超氧化物歧化酶（SOD）和过氧化物酶（POD）活性，分析NaCl胁迫对红豆和白扁豆种子萌发及幼苗生长的影响。结果表明：1）随NaCl浓度增加，红豆和白扁豆种子发芽率、发芽势、发芽指数和活力指数均呈下降趋势。当NaCl浓度为80 mmol·L-1时，白扁豆发芽势、发芽指数、活力指数降为0，红豆的发芽势、发芽指数、活力指数分别为20.00%、2.00、0.83；NaCl浓度为100 mmol·L-1时，红豆的发芽率为16.67%，但白扁豆为0，这表明在盐胁迫下红豆较白扁豆具有更高的萌发能力。2）红豆与白扁豆相对盐害率随NaCl浓度的增加而增加，当NaCl浓度为80 mmol·L-1和100 mmol·L-1时，白扁豆相对盐害率为96.58%和96.67%，红豆相对盐害率为47.05%和83.18%，说明红豆受盐害程度较低。3）红豆与白扁豆幼苗胚根、胚芽及鲜重均随NaCl浓度增加而下降。NaCl浓度为100 mmol·L-1时，白扁豆胚根长为0，红豆胚根长为0.23 cm。4）随NaCl浓度升高，红豆和白扁豆叶片的MDA含量均增加，造成细胞膜透性逐渐增大，但是红豆幼苗MDA积累量低于白扁豆，这表明红豆叶片细胞膜损伤较小。5）NaCl胁迫下，红豆与白扁豆SOD活性均显著升高，但红豆SOD活性显著高于白扁豆；NaCl胁迫下，POD活性显著升高，但白扁豆POD活性显著下降。研究发现红豆可通过提高SOD和POD活性以降低细胞膜氧化伤害，减少MDA积累量，进而提高种子萌发能力。在相同浓度NaCl胁迫下红豆较白扁豆有更高的耐盐性，能更好地适应盐胁迫环境。
关键词:盐胁迫/
耐盐性/
保护酶活性/
种子萌发/
红豆/
白扁豆
Abstract:Soil salinization has an important effect on seed germination of plants; however, little attention has given to the effect of salt stress on the seed germination of legumes. The Vigna angularis variety 'Yuhongdou 2' and traditional Dolichos lablab variety were used to determine the germination percentage, germination potential, germination index, vigor index, radicle length, embryo length, and fresh weight, malondialdehyde (MDA) content, and superoxide dismutase (SOD) and peroxidase (POD) activity under different NaCl concentrations (0 mmol·L-1, 20 mmol·L-1, 40 mmol·L-1, 60 mmol·L-1, 80 mmol·L-1, 100 mmol·L-1) in an artificial climate chest. The goal of this study was to investigate the relationship between osmotic regulators, stress resistance enzymes, and salt tolerance of leguminous plants under NaCl stress. The results were as follows:1) salinity stress inhibited the germination index of V. angularis. Then germination percentage, germination potential, germination index, and vigor index of V. angularis decreased with increasing NaCl stress and was significantly higher than that of D. lablab. 2) The relative salt damage rate of V. angularis and D. lablab increased with the increase in NaCl concentration. When the NaCl concentration was 80 mmol·L-1 and 100 mmol·L-1, the relative salt damage rate of D. lablab was 96.58% and 96.67%, whereas the relative salt damage rate of V. angularis was 47.05% and 83.18%, indicating that the salt damage to V. angularis was less intense. 3) The radicle length, embryo length, and fresh weight of V. angularis and D. lablab seedlings decreased with the increase of NaCl concentration. When NaCl concentration was 100 mmol·L-1, the radicle length of D. lablab was 0, and that of V. angularis was 0.23 cm. 4) With the increase of NaCl concentration, the content of MDA in the leaves of V. angularis and D. lablab increased, resulting in a gradual increase in cell membrane permeability, but the accumulation of MDA in V. angularis seedlings was lower than that in D. lablab, which indicated that the cell membrane of V. angularis leaves was less damaged. 5) Under NaCl stress, SOD activity of V. angularis and D. lablab increased significantly, but SOD activity of V. angularis was significantly higher than that of D. lablab. Under NaCl stress, POD activity of V. angularis increased significantly, but the POD activity of D. lablab decreased significantly. It was found that V. angularis could reduce the oxidative damage of the cell membrane and the accumulation of MDA by increasing SOD and POD activities, thus improving the germination ability of seeds. V. angularis was higher in salt tolerance than D. lablab under the same concentration of NaCl stress and can better adapt to a salt stress environment.
Key words:Salt stress/
Salt tolerance/
Protective enzyme activity/
Seed germination/
Vigna angularis/
Dolichos lablab
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图1不同NaCl浓度对红豆和白扁豆发芽率(A)、发芽势(B)、发芽指数(C)和活力指数(D)的影响
不同大写字母表示不同盐浓度下红豆性状差异显著, 不同小写字母表示不同盐浓度下白扁豆性状差异显著(按照Duncon的统计检验, P < 0.05)。ns和*、**分别表示相同盐浓度下红豆与白扁豆间的差异不显著(P > 0.05)和在P < 0.05、P < 0.01水平差异显著。
Figure1.Effects of different NaCl concentrations on the germination rates (A), germination energies (B), germination indexes (C) and vigor indexes (D) of Vigna angularis and Dolichos lablab
Different capital letters indicate significant differences among different NaCl concentrations of Vigna angularis, different lowercase letters indicate significant differences among different NaCl concentrations of Dolichos lablab (according to Duncon's statistical test, P < 0.05). "ns" and "*", "**" mean no significant difference (P > 0.05) and significant differences at P < 0.05, P < 0.01 between Vigna angularis and Dolichos lablab under the same NaCl concentration, respectively.
下载: 全尺寸图片幻灯片
图2不同NaCl浓度下红豆和白扁豆的相对盐害率
不同大写字母表示不同盐浓度下红豆性状差异显著, 不同小写字母表示不同盐浓度下白扁豆性状差异显著(按照Duncon的统计检验, P < 0.05)。*、**分别表示相同盐浓度下红豆与白扁豆间在P < 0.05、P < 0.01水平差异显著。
Figure2.Relative salt injury rates of Vigna angularis and Dolichos lablab under different NaCl concentrations
Different capital letters indicate significant differences among different NaCl concentrations of Vigna angularis, different lowercase letters indicate significant differences among different NaCl concentrations of Dolichos lablab (according to Duncon's statistical test, P < 0.05). "*", "**" mean significant differences at P < 0.05 and P < 0.01, respectively, between Vigna angularis and Dolichos lablab under the same NaCl concentration.
下载: 全尺寸图片幻灯片
图3不同NaCl浓度对红豆和白扁豆幼苗MDA含量(A)和POD(B)、SOD(C)活性的影响
不同大写字母表示不同盐浓度下红豆性状差异显著, 不同小写字母表示不同盐浓度下白扁豆性状差异显著(按照Duncon的统计检验, P < 0.05)。ns和*、**分别表示相同盐浓度下红豆与白扁豆间的差异不显著(P > 0.05)和在P < 0.05、P < 0.01水平差异显著。
Figure3.Effects of different NaCl concentrations on MDA content (A) and activities of POD (B) and SOD (C) of Vigna angularis and Dolichos lablab seedlings
Different capital letters indicate significant differences among different NaCl concentrations of Vigna angularis, different lowercase letters indicate significant differences among different NaCl concentrations of Dolichos lablab (according to Duncon's statistical test, P < 0.05). "ns" and "*", "**" mean no significant difference (P > 0.05) and significant differences at P < 0.05, P < 0.01 between Vigna angularis and Dolichos lablab under the same NaCl concentration, respectively.
下载: 全尺寸图片幻灯片表1不同NaCl浓度对红豆和白扁豆幼苗胚根长、胚芽长、鲜重的影响
Table1.Effects of different NaCl concentrations on radicle length, embryo length and fresh weight of Vigna angularis and Dolichos lablab seedlings
| NaCl浓度 NaCl concentration (mmol?L-1) | 红豆?Vigna angularis (VA) | 白扁豆? Dolichos lablab (DL) | |||||
| 胚根长 Radicle length (cm) | 胚芽长 Embryo length (cm) | 地上部鲜重 Fresh weight (g) | 胚根长 Radicle length (cm) | 胚芽长 Embryo length (cm) | 地上部鲜重 Fresh weight (g) | ||
| 0 | 5.03±0.17ans | 16.30±0.67a* | 1.03±0.03ans | 4.87±0.07a | 14.07±0.77a | 1.02±0.01a | |
| 20 | 4.10±0.12b* | 3.03±0.38bns | 0.83±0.01b* | 3.10±0.21b | 2.37±0.58b | 0.70±0.05b | |
| 40 | 2.30±0.17c* | — | — | 2.77±0.03b | — | — | |
| 60 | 0.67±0.07d* | — | — | 1.17±0.33c | — | — | |
| 80 | 0.40±0.06de* | — | — | 0.20±0.06d | — | — | |
| 100 | 0.23±0.03e** | — | — | 0 | — | — | |
| 胚根长?Radicle length | 胚芽长?Embryo length | 鲜重?Fresh weight | |||||
| PVA | 0.000 | 0.000 | 0.001 | ||||
| PDL | 0.000 | 0.000 | 0.000 | ||||
| PVA×DL | 0.000 | — | — | ||||
| 同列不同小写字母表示不同盐浓度间差异显著(按照Duncon的统计检验, P < 0.05)。ns和*、**分别表示相同盐浓度下红豆与白扁豆间的差异不显著(P > 0.05)和在P < 0.05、P < 0.01水平差异显著。Different lowercase letters indicate significant differences among different NaCl concentrations (according to Duncon’s statistical test, P < 0.05). “ns” and “*”, “**” mean no significant difference (P > 0.05) and significant differences at P < 0.05 and P < 0.01, respectively, between Vigna angularis and Dolichos lablab under the same NaCl concentration. | |||||||
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