陈杰^{1, 2,},
马永清^3,,,
郭振国⁴,
薛泉宏¹
1.西北农林科技大学资源环境学院 杨凌 712100
2.山西农业大学农学院 太谷 030801
3.中国科学院水利部水土保持研究所黄土高原土壤侵蚀与旱地农业国家重点实验室 杨凌 712100
4.西北农林科技大学林学院 杨凌 712100
基金项目: 新疆生产建设兵团农业与社会发展领域科技计划项目2016AC007

详细信息

作者简介:陈杰, 主要研究方向为土壤微生物。E-mail:chenjie03306@163.com

通讯作者:马永清, 主要研究方向为寄生植物与植物化感作用。E-mail:mayongqing@ms.iswc.ac.cn

中图分类号:Q939.96

计量

文章访问数:676
HTML全文浏览量:5
PDF下载量:569
被引次数:0

出版历程

收稿日期:2018-10-10
录用日期:2018-12-20
刊出日期:2019-05-01

Effect of Penicillium griseofulvum on control of Orobanche aegyptiaca and microorganisms in rhizosphere soils of tomato

CHEN Jie^{1, 2,},
MA Yongqing^3,,,
GUO Zhenguo⁴,
XUE Quanhong¹
1. College of Natural Resources and Environment, Northwest A & F University, Yangling 712100, China
2. College of Agriculture, Shanxi Agricultural University, Taigu 030801, China
3. The State Key Laboratory of Soil Erosion and Dryland Farming, Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling 712100, China
4. College of Forestry, Northwest A & F University, Yangling 712100, China
Funds: Science and Technology Plan for the Field of Agriculture and Social Development by the Xinjiang Production and Construction Corps2016AC007

More Information

Corresponding author:Corresponding author, E-mail:mayongqing@ms.iswc.ac.cn

摘要
HTML全文
图(2)表(4)
参考文献(29)
相关文章
施引文献
资源附件(0)
访问统计

摘要
摘要:根寄生杂草瓜列当（Orobanche aegyptiaca）严重危害番茄（Solanum lycopersicum）等多种经济作物的产量和品质。如何有效防除仍是当今瓜列当研究重点之一。真菌是列当的生防因子之一，但目前对农作物无致病性的列当生防真菌的研究尚少。本研究通过培养皿试验研究1株灰黄青霉（Penicillium griseofulvum，CF3）的无细胞发酵滤液对瓜列当种子萌发和发芽管生长的影响，通过盆栽试验研究CF3粉状制剂对瓜列当的防除效果及对寄主番茄生长和根区土壤微生物的影响。结果表明：1）培养皿试验中，CF3发酵液抑制了瓜列当种子萌发和发芽管生长。其中，在放有瓜列当种子与番茄幼苗的培养皿中，加入CF3发酵液后培养6 d，瓜列当种子的萌发均被完全抑制；添加CF3发酵液与霍格兰德营养液体积比为1:2、1:4、1:6和1:8的混合液培养8 d后，瓜列当种子的萌发率与对照相比分别减少80.26%、70.26%、68.10%和47.51%。CF3发酵液原液、10倍稀释液和100倍稀释液处理后使瓜列当发芽管长度与对照相比分别缩短100.00%、68.84%和19.24%。2）盆栽试验中，CF3菌剂抑制了瓜列当的出土和单株瓜列当的生长，并使番茄增产。施加1.0 g·kg^-1 CF3菌剂130 d后，瓜列当的出土数量、出土率和单株瓜列当干重分别降低76.19%、85.30%和28.48%，番茄果实鲜重增加51.57%。此外，灰黄青霉菌剂还调整了番茄根区土壤的微生物区系结构，使施加菌剂130 d后番茄根区土壤中除接入CF3外真菌数量与对照相比降低75.60%，细菌与真菌的数量之比增加117.57%。平均来看，CF3使番茄根区土壤中除CF3外真菌数量降低42.81%，放线菌总数增加84.15%。本研究表明，灰黄青霉CF3具有防除番茄上寄生瓜列当的能力，适宜作为瓜列当的生防真菌。
关键词:瓜列当/
灰黄青霉/
真菌/
生物防治/
土壤微生物
Abstract:Root parasitic weed Orobanche aegyptiaca adversely affects yield and quality of tomato (Solanum lycopersicum). The means of effective control is still the focus in O. aegyptiaca research. Fungus is one of the biocontrol agents of Orobanche spp.. However, few studies have been done on the use of non-pathogentic fungi to control Orobanche spp. weed. In this study, the effects of cell-free culture filtrate of Penicillium griseofulvum, a non-pathogentic fungus strain of O. aegyptiaca, on O. aegyptiaca seed germination and germ tube growth were investigated in a petri-dish experiment. In addition, a pot experiment was conducted to explore the effect of powdered P. griseofulvum inoculum on weedy O. aegyptiaca control. The effects of P. griseofulvum inoculum on the growth of host tomato plants and the change in microflora in rhizosphere soils of tomato plants were also investigated. Results showed that:1) in the petri-dish experiment, cell-free culture filtrate of P. griseofulvum significantly inhibited both O. aegyptiaca seed germination and germ tube growth. When O. aegyptiaca seeds and tomato seedlings were co-cultured for 6 days, O. aegyptiaca seed germination was completely inhibited (100.0%) in treatments with P. griseofulvum cell-free culture filtrate. After co-culturing for 6 days, O. aegyptiaca seed germination rates reduced by 80.26%, 70.26%, 68.10% and 47.51%, respectively in treatments with volume ratios of P. griseofulvum cell-free culture filtrate and Hogland nutrient solution ratios of 1:2, 1:4, 1:6 and 1:8. The lengths of O. aegyptiaca germ tubes significantly reduced by 100.00%, 68.84% and 19.24%, respectively when treated by undiluted, 10-fold diluted and 100-fold diluted P. griseofulvum cell-free culture filtrate. 2) In the pot experiment, P. griseofulvum inoculum inhibited the emergence of O. aegyptiaca tubercles and the growth of individual O. aegyptiaca tubercle, but simultaneously increased tomato fruit yield. The number of epigeal O. aegyptiaca tubercles, epigeal rate of O. aegyptiaca tubercles and dry weight of individual O. aegyptiaca tubercles all significantly reduced after the application of powdered P. griseofulvum inoculum at 1.0 g·kg^-1 for 130 days respectively by 76.19%, 85.30% and 28.48% than the control. After the application of P. griseofulvum inoculum for 130 days, tomato fruit yield was 346.8 g per plant (51.57%) more than the control (228.8 g). In addition, P. griseofulvum also adjusted microflora structure in rhizosphere soils of tomato plants. The application of P. griseofulvum inoculum reduced fungi population (excluding CF3) and significantly increased population ratio of bacteria to fungi in rhizosphere soils of tomato plants by 75.60% and 117.57%, respectively, compared with the control. On average, application of P. griseofulvum inoculant reduced fungi population (excluding P. griseofulvum) and increased actinomycetes population in rhizosphere soils of tomato plants respectively by 42.81% and 84.15% over the control. In conclusion, P. griseofulvum had the ability to control O. aegyptiaca infection of tomato plant with fungus as suitable biological agent to control O. aegyptiaca.
Key words:Orobanche aegyptiaca/
Penicillium griseofulvum/
Fungus/
Biocontrol/
Soil microorganism

HTML全文


图1灰黄青霉CF3发酵液对瓜列当发芽管长度的抑制作用
CK: 0.1 mg∙L^-1 GR24处理。图中误差线为标准差(n=30)。图中不同小写字母表示处理间数值经Tukey检验差异显著(P < 0.05)。
Figure1.Inhibition effect of the cell-free culture filtrate of Penicillium griseofulvum CF3 on the germ tube elongation of Orobanche aegyptiaca
CK: treated by 0.1 mg∙L^-1 GR24. Bars in the figure mean standard deviation (n = 30). Different lowercase letters in the figure indicate significant differences among treatments (P < 0.05) based on the Tukey's test.


下载: 全尺寸图片幻灯片


图2灰黄青霉CF3发酵液对瓜列当发芽管形态的影响
a、b、c和CK分别为CF3发酵液原液、10倍、100倍稀释液和0.1 mg∙L^-1 GR24处理10 d后的瓜列当发芽管形态。
Figure2.Effects of the cell-free culture filtrate of Penicillium griseofulvum CF3 on the form of Orobanche aegyptiaca germ tubes
a, b, c and CK are the forms of O. aegyptiaca germ-tubes treated by the un-diluted, 10 fold, 100 fold diluted cell-free culture filtrates of P. griseofulvum CF3, and GR24 at 0.1 mg∙L^-1 for 10 days, respectively.


下载: 全尺寸图片幻灯片

表1灰黄青霉CF3发酵液对番茄诱导瓜列当种子萌发的抑制作用
Table1.Inhibition effect of the cell-free culture filtrate of Penicillium griseofulvum CF3 on seed germination of Orobanche aegyptiaca induced by tomato

CF3发酵液与Hogland营养液体积比 VCF3∶VHogland	培养时间Cultivated time (d)
	6			8			10			12			14
	发芽率 RG (%)	抑制率 RP (%)		发芽率 RG (%)	抑制率 RP (%)		发芽率 RG (%)	抑制率 RP (%)		发芽率 RG (%)	抑制率 RP (%)		发芽率 RG (%)	抑制率 RP (%)
CK	23.48±1.46a	—		33.27±4.44a	—		56.70±6.35a	—		64.55±4.16a	—		77.14±3.46a	—
1∶2	0.00±0.00b	100.00		6.57±0.54c	80.26		31.56±3.06c	45.58		55.84±8.57a	13.57		62.27±4.21b	19.23
1∶4	0.00±0.00b	100.00		9.90±0.52bc	70.26		43.56±2.89b	24.89		56.23±5.72a	12.96		65.70±7.48ab	14.79
1∶6	0.00±0.00b	100.00		10.62±5.08bc	68.10		43.62±3.79b	24.80		65.24±1.60a	-1.00		77.52±3.47a	-0.55
1∶8	0.00±0.00b	100.00		17.48±3.61b	47.51		46.11±5.33ab	20.50		63.87±5.26a	1.13		72.31±5.86ab	6.21
??表中数据为平均值±标准差(n=3)。同列数据后不同小写字母表示处理间数值经反正弦转换再经Tukey检验后差异显著(P < 0.05)VCF3: volume of cell-free culture filtrate of Penicillium griseofulvuml CF3; VHogland: Hogland solution volume; RG: germination rate; RP: percent reduction. Values are means ± standard deviation (n = 3). Different lowercase letters in the same column indicate significant differences (arcsine transformed) among treatments (P < 0.05) based on the Tukey’s test.

下载: 导出CSV
表2盆栽试验中灰黄青霉CF3菌剂对瓜列当寄生数量和生物量的影响
Table2.Effects of the Penicillium griseofulvum CF3 inoculum on parasitism and biomass of Orobanche aegyptiaca in the pot experiment

采样时期 Sampling time	处理 Treatment	出土数量 Epigeal number			寄生总数 Total parasitism number			出土率 Epigeal rate			总干重 Total dry weight			单株干重 Individual dry weight
		测值 Measured value (plant?pot-1)	增率 ΔCK (%)		测值 Measured value (plant?pot-1)	增率 ΔCK (%)		测值 Measured value (%)	增率 ΔCK (%)		测值 Measured value (g?pot-1)	增率 ΔCK (%)		测值 Measured value (g?plant-1)	增率 ΔCK (%)
移栽后70 d 70 days after transplantation	CK	0.67±0.58	—		3.00±1.73	—		23.33±25.17	—		2.48±0.30	—		0.96±0.36	—
	CF3	0.33±0.58	-50.00		5.00±5.57	66.67		3.03±5.25	-87.01		1.06±1.00	-57.12		0.16±0.15	-83.28*
移栽后130 d 130 days after transplantation	CK	7.00±1.00	—		8.67±1.53	—		82.22±16.78	—		8.39±1.01	—		0.98±0.12	—
	CF3	1.67±0.58	-76. 19*		13.67±0.58	57.69*		12.09±3.81	-85.30*		9.56±0.49	13.95		0.70±0.05	-28.48*
平均值 Mean	CK	3.83±3.54	—		5.83±3.43	—		52.78±37.50	—		5.44±3.30	—		0.97±0.24	—
	CF3	1.00±0.89	-73.91		9.33±5.92	60.00		5.18±6.08	-90.19*		5.31±4.71	-2.27		0.43±0.31	-55.64*
??CF3:灰黄青霉。表中数据为平均值±标准差(n=3或6)。*表示处理与对照经t检验差异显著(P < 0.05)。CF3: P. griseofulvum. Values are means ± standard deviation (n =3 or 6). * in the table indicates significant difference (P < 0.05) between CF3 and CK treatments based on the t test.

下载: 导出CSV
表3盆栽试验中灰黄青霉CF3菌剂对番茄生长的影响
Table3.Promotion effects of the Penicillium griseofulvum CF3 inoculum on the growth of tomato plants in the pot experiment

采样时期 Sampling time	处理 Treatment	株高 Plant height			茎叶干重 Dry weight of stems and leaves			果实个数 Number of fruits			果实鲜重 Total fresh weight of fruits			根系干重 Dry weight of roots
		测值 Measured value (cm)	增率 ΔCK (%)		测值 Measured value (g?plant-1)	增率 ΔCK (%)		测值 Measured value (number?plant-1)	增率 ΔCK (%)		测值 Measured value (g?plant-1)	增率 ΔCK (%)		测值 Measured value (g?plant-1)	增率 ΔCK (%)
移栽后70 d 70 days after transplantation	CK	43.00±1.00	—		11.68±2.75	—		3.00±0.00	—		135.17±6.86	—		—	—
	CF3	45.00±6.24	4.65		8.69±1.03	-25.60		6.00±1.73	100*		107.83±23.03	-20.23		—	—
移栽后130 d 130 days after transplantation	CK	48.97±7.60	—		16.60±0.52	—		6.00±2.00	—		228.82±7.13	—		1.95±0.31	—
	CF3	62.50±4.50	27.64*		16.64±0.53	0.24		9.00±1.00	50.00		346.81±40.10	51.57*		2.20±0.32	12.46
平均值 Mean	CK	45.98±5.85	—		14.14±3.22	—		4.50±2.07	—		181.99±51.67	—		—	—
	CF3	53.75±10.75	16.89		12.67±4.42	-10.43		7.50±2.07	66.67*		227.32±134.12	24.91		—	—
??CF3:灰黄青霉。表中数据为平均值±标准差(n=3或6)。*表示处理数值与相应对照相比经t检验差异显著(P<0.05)。CF3: P. griseofulvum. Values are means ± standard deviation (n = 3 or 6). * in the table indicates significant difference (P < 0.05) between CF3 and CK treatments based on the t test.

下载: 导出CSV
表4盆栽试验中灰黄青霉CF3菌剂对番茄根区土壤微生物区系的影响
Table4.Effects of the Penicillium griseofulvum CF3 inoculum on microflora in the rhizosphere soils of tomato plants in the pot experiment

采样时期 Sampling time	处理 Treat-ment	细菌 Bacteria				真菌Fungi					放线菌 Actinomycetes			细菌数量/真菌数量 Bacteria/fungi			放线菌数量/真菌数量 Actinomycetes/ fungi
				CF3 P. griseofulvum				除CF3外真菌 Fungi besides CF3
		数量 Amount (105 CFU?g-1)	增率 ΔCK (%)		数量 Amount (103 CFU?g-1)	定殖率 Colonization rate (%)		数量 Amount (103 CFU?g-1)	增率 ΔCK (%)		数量 Amount (105 CFU?g-1)	增率 ΔCK (%)		比值 Ratio	增率 ΔCK (%)		比值 Ratio	增率 ΔCK (%)
移栽后70 d 70 days after transplantation	CK	6.19±2.14	—		—	—		27.23±2.84	—		11.57±3.64	—		23.31±9.59	—		42.12±9.88	—
	CF3	15.00±3.75	142.35*		32.49±1.08	54.23		27.49±2.86	0.98		26.18±2.24	126.26*		25.08±6.56	7.58		43.65±3.62	3.64
移栽后130 d 130 days after transplantation	CK	5.02±0.52	—		—	—		36.36±5.19	—		8.37±0.50	—		13.94±1.94	—		23.45±4.52	—
	CF3	4.71±0.42	-6.12		7.76±2.54	46.34		8.87±2.54	-75.60*		10.54±1.92	25.91		30.33±10.02	117.57*		69.49±30.77	196.37
平均值 Mean	CK	5.60±1.53	—		—	—		31.79±6.25	—		99.70±29.14	—		18.63±8.04	—		32.78±12.32	—
	CF3	9.86±6.12	75.84		20.13±13.66	50.29		18.18±10.48	-42.81*		183.59±87.69	84.15*		27.71±8.10	48.74		56.57±24.17	72.56
??CF3: CF3:灰黄青霉。表中数据为平均值±标准差(n=3或6)。*表示处理数值与相应对照相比经t检验差异显著(P < 0.05)。CF3: P. griseofulvum. Values are means ± standard deviation (n = 3 or 6). * indicates significant difference (P < 0.05) between CF3 and CK treatments based on the t test.

下载: 导出CSV

参考文献(29)

[1]	PARKER C. The parasitic weeds of the Orobanchaceae[M]//JOEL D M, GRESSEL J, MUSSELMAN L J. Parasitic Orobanchaceae. Berlin, Heidelberg:Springer, 2013:313-344
[2]	ZHANG X K, YAO Z Q, ZHAO S F, et al. Rhizopus stem rot of Orobanche aegyptiaca caused by Rhizopus oryzae in China[J]. Journal of Phytopathology, 2013, 161(10):745-748 doi: 10.1111/jph.2013.161.issue-10
[3]	张学坤, 姚兆群, 赵思峰, 等.分枝(瓜)列当在新疆的分布、危害及其风险评估[J].植物检疫, 2012, 26(6):31-33 http://d.old.wanfangdata.com.cn/Periodical/zwjy201206010 ZHANG X K, YAO Z Q, ZHAO S F, et al. Distribution, harmfulness and its assessment of Orobanche aegyptiaca in Xinjiang Province[J]. Plant Quarantine, 2012, 26(6):31-33 http://d.old.wanfangdata.com.cn/Periodical/zwjy201206010
[4]	陈杰, 马永清, 薛泉宏.利用微生物防除根寄生杂草列当[J].中国生态农业学报, 2018, 26(1):49-56 http://www.ecoagri.ac.cn/zgstny/ch/reader/view_abstract.aspx?file_no=20180106&flag=1 CHEN J, MA Y Q, XUE Q H. Use of microorganisms in controlling parasitic root weed Orobanche spp.[J]. Chinese Journal of Eco-Agriculture, 2018, 26(1):49-56 http://www.ecoagri.ac.cn/zgstny/ch/reader/view_abstract.aspx?file_no=20180106&flag=1
[5]	THOMAS H, SAUERBORN J, MULLER-ST VER D, et al. The potential of Fusarium oxysporum f. sp. orthoceras as a biological control agent for Orobanche cumana in sunflower[J]. Biological Control, 1998, 13(1):41-48 doi: 10.1006/bcon.1998.0642
[6]	NEMAT ALLA M M, SHABANA Y M, SERAG M M, et al. Granular formulation of Fusarium oxysporum for biological control of faba bean and tomato Orobanche[J]. Pest Management Science, 2008, 64(12):1237-1249 doi: 10.1002/ps.v64:12
[7]	DOR E, HERSHENHORN J. Evaluation of the pathogenicity of microorganisms isolated from Egyptian broomrape (Orobanche aegyptiaca) in Israel[J]. Weed Biology and Management, 2009, 9(3):200-208 doi: 10.1111/wbm.2009.9.issue-3
[8]	DOR E, HERSHENHORN J, ANDOLFI A, et al. Fusarium verticillioides as a new pathogen of the parasitic weed Orobanche spp.[J]. Phytoparasitica, 2009, 37(4):361-370 doi: 10.1007/s12600-009-0049-0
[9]	LOUARN J, CARBONNE F, DELAVAULT P, et al. Reduced germination of Orobanche cumana seeds in the presence of arbuscular mycorrhizal fungi or their exudates[J]. PLoS One, 2012, 7(11):e49273 doi: 10.1371/journal.pone.0049273
[10]	FERNáNDEZ-APARICIO M, GARC A-GARRIDO J M, OCAMPO J A, et al. Colonisation of field pea roots by arbuscular mycorrhizal fungi reduces Orobanche and Phelipanche species seed germination[J]. Weed Research, 2010, 50(3):262-268 doi: 10.1111/j.1365-3180.2010.00771.x
[11]	El-KASSAS R, El-DIN Z K, BEALE M H, et al. Bioassay-led isolation of Myrothecium verrucaria and verrucarin A as germination inhibitors of Orobanche crenata[J]. Weed Research, 2005, 45(3):212-219 doi: 10.1111/wre.2005.45.issue-3
[12]	申光辉, 薛泉宏, 张晶, 等.草莓根腐病拮抗真菌筛选鉴定及其防病促生作用[J].中国农业科学, 2012, 45(22):4612-4626 doi: 10.3864/j.issn.0578-1752.2012.22.007 SHEN G H, XUE Q H, ZHANG J, et al. Screening, identification and biocontrol potential of antagonistic fungi against strawberry root rot and plant growth promotion[J]. Scientia Agricultura Sinica, 2012, 45(22):4612-4626 doi: 10.3864/j.issn.0578-1752.2012.22.007
[13]	CHEN J, XUE Q H, MCERLEAN C S P, et al. Biocontrol potential of the antagonistic microorganism Streptomyces enissocaesilis against Orobanche cumana[J]. BioControl, 2016, 61(6):781-791 doi: 10.1007/s10526-016-9738-z
[14]	HOAGLAND D R, ARNON D I. The Water-culture Method for Growing Plants Without Soil[M]. Berkeley, USA:University of California, 1950:39
[15]	程丽娟, 薛泉宏.微生物学实验技术[M].北京:科学出版社, 2012:80-84, 383-384 CHENG L J, XUE Q H.Laboratory Manual of Microbiology[M]. Beijing:Science Press, 2012:80-84, 383-384
[16]	BARGHOUTHI S, SALMAN M. Bacterial inhibition of Orobanche aegyptiaca and Orobanche cernua radical elongation[J]. Biocontrol Science and Technology, 2010, 20(4):423-435 doi: 10.1080/09583150903340544
[17]	CIMMINO A, FERN NDEZ-APARICIO M, ANDOLFI A, et al. Effect of fungal and plant metabolites on broomrapes (Orobanche and Phelipanche spp.) seed germination and radicle growth[J]. Journal of Agricultural and Food Chemistry, 2014, 62(43):10485-10492 doi: 10.1021/jf504609w
[18]	CHEN J, WEI J, GAO J M, et al. Allelopathic inhibitory effects of Penicillium griseofulvum produced patulin on the seed germination of Orobanche cumana Wallr. and Phelipanche aegyptiaca Pers.[J]. Allelopathy Journal, 2017, 41(1):65-80 doi: 10.26651/AJ
[19]	MüLLER-ST?VER D, KOHLSCHMID E, SAUERBORN J. A novel strain of Fusarium oxysporum from Germany and its potential for biocontrol of Orobanche ramosa[J]. Weed Research, 2009, 49(2):175-182 doi: 10.1111/j.1365-3180.2008.00686.x
[20]	BOARI A, VURRO M. Evaluation of Fusarium spp. and other fungi as biological control agents of broomrape (Orobanche ramosa)[J]. Biological Control, 2004, 30(2):212-219 doi: 10.1016/j.biocontrol.2003.12.003
[21]	ZERMANE N, SOUISSI T, KROSCHEL J, et al. Biocontrol of broomrape (Orobanche crenata Forsk. and Orobanche foetida Poir.) by Pseudomonas fluorescens isolate Bf 7-9 from the faba bean rhizosphere[J]. Biocontrol Science and Technology, 2007, 17(5):483-497 doi: 10.1080/09583150701309535
[22]	MUSSELMAN L J. The biology of Striga, Orobanche, and other root-parasitic weeds[J]. Annual Review of Phytopathology, 1980, 18(1):463-489 doi: 10.1146/annurev.py.18.090180.002335
[23]	EL-TARABILY K A, NASSA A H, HARDY G E ST J, et al. Plant growth promotion and biological control of Pythium aphanidermatum, a pathogen of cucumber, by endophytic actinomycetes[J]. Journal of Applied Microbiology, 2009, 106(1):13-26 doi: 10.1111/jam.2008.106.issue-1
[24]	段佳丽, 舒志明, 孙群, 等.放线菌剂对丹参生长及有效成分的影响[J].西北农林科技大学学报:自然科学版, 2012, 40(2):195-200 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=xbnydxxb201202030 DUAN J L, SHU Z M, SUN Q, et al. Effect of antimicrobial actinomycetes on growth and medicine quality of Salvia miltiorrhiza Bge.[J] Journal of Northwest A & F University:Natural Science Edition, 2012, 40(2):195-200 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=xbnydxxb201202030
[25]	赵娟, 杜军志, 薛泉宏, 等. 3株放线菌对甜瓜幼苗的促生与抗性诱导作用[J].西北农林科技大学学报:自然科学版, 2010, 38(2):109-116 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=xbnydxxb201002018 ZHAO J, DU J Z, XUE Q H, et al. The growth-promoting effect and resistance induction of 3 antagonistic actinomyces on Cucumis melo L.[J]. Journal of Northwest A & F University:Nat. Sci. Ed., 2010, 38(2):109-116 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=xbnydxxb201002018
[26]	HRISTEVA T, DEKALSKA T, DENEV L. Structural and functional biodiversity of microbial communities in the rhizosphere of plants infected with broomrapes (Orobanch aceae)[J]. Biotechnology & Biotechnological Equipment, 2013, 27(5):4082-4086
[27]	孙敬祖, 薛泉宏, 唐明, 等.放线菌制剂对连作草莓根区微生物区系的影响及其防病促生作用[J].西北农林科技大学学报:自然科学版, 2009, 37(12):153-158 http://www.cnki.com.cn/Article/CJFDTOTAL-XBNY200912026.htm SUN J Z, XUE Q H, TANG M, et al. Study on the effect of actinomycetes on microflora of replanted strawberry's root domain and the bio-control effectiveness[J]. Journal of Northwest A & F University:Natural Science Edition, 2009, 37(12):153-158 http://www.cnki.com.cn/Article/CJFDTOTAL-XBNY200912026.htm
[28]	BERNARD E, LARKIN R P, TAVANTZIS S, et al. Compost, rapeseed rotation, and biocontrol agents significantly impact soil microbial communities in organic and conventional potato production systems[J]. Applied Soil Ecology, 2012, 52:29-41 doi: 10.1016/j.apsoil.2011.10.002
[29]	张鸿雁, 薛泉宏, 申光辉, 等.放线菌制剂对人参生长及根域土壤微生物区系的影响[J].应用生态学报, 2013, 24(8):2287-2293 http://d.old.wanfangdata.com.cn/Periodical/yystxb201308027 ZHANG H Y, XUE Q H, SHEN G H, et al. Effects of actinomycetes agent on ginseng growth and rhizosphere soil microflora[J]. Chinese Journal of Applied Ecology, 2013, 24(8):2287-2293 http://d.old.wanfangdata.com.cn/Periodical/yystxb201308027