张玉铭2,,,
赵宝华1,,,
胡春胜2,
何红波3,
董文旭2,
王玉英2,
李晓欣2
1.河北师范大学生命科学学院 石家庄 050024
2.中国科学院遗传与发育生物学研究所农业资源研究中心/中国科学院 农业水资源重点实验室/河北省土壤生态学重点实验室 石家庄 050022
3.中国科学院沈阳应用生态研究所 沈阳 110016
基金项目: 国家重点研发计划项目2016YFD0200307
国家重点研发计划项目2016YFD0300808
国家自然科学基金项目41571291
详细信息
作者简介:李俊娣, 主要研究方向为农田生态系统氮素物理保护机制及生物保护机制。E-mail:jundili@163.com
通讯作者:张玉铭, 主要研究方向为农田生态系统养分循环与平衡及其环境效应, E-mail：ymzhang@sjziam.ac.cn
赵宝华, 主要从事微生物技术及应用研究, E-mail：zhaobaohua@hebtu.edu.cn
中图分类号:S153;S154.36计量
文章访问数:823
HTML全文浏览量:6
PDF下载量:1026
被引次数:0
出版历程
收稿日期:2019-01-30
录用日期:2019-02-25
刊出日期:2019-04-01
Effect of long-term addition of organic substances on soil nitrogen and amino sugars in particle-size fractions in the North China Plain
LI Jundi1, 2,,ZHANG Yuming2,,,
ZHAO Baohua1,,,
HU Chunsheng2,
HE Hongbo3,
DONG Wenxu2,
WANG Yuying2,
LI Xiaoxin2
1. College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China
2. Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences/Key Laboratory of Agricultural Water Resources, Chinese Academy of Sciences/Hebei Key Laboratory of Soil Ecology, Shijiazhuang 050022, China
3. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
Funds: the National Key Research and Development Program of China2016YFD0200307
the National Key Research and Development Program of China2016YFD0300808
the National Natural Science Foundation of China41571291
More Information
Corresponding author:ZHANG Yuming, E-mail: ymzhang@sjziam.ac.cn;ZHAO Baohua, E-mail: zhaobaohua@hebtu.edu.cn
摘要
HTML全文
图
参考文献
相关文章
施引文献
资源附件
访问统计
摘要
摘要:华北平原是我国主要的粮食生产基地之一，农民为了追求高产，过量施用化肥的弊端日益凸显。本研究依托中国科学院栾城农业生态系统试验站有机养分循环再利用长期定位试验，开展不同外源有机物料对土壤氮素和氨基糖在不同粒级土壤库中分布的影响研究，为阐释不同农业管理措施下土壤氮素的物理保护机制和生物保护机制提供依据。定位试验设6个处理：无肥无秸秆处理（对照，CK）、单施猪圈肥（M）、单施化肥（NPK）、单施秸秆（SCK）、化肥配施猪圈肥（MNPK）和化肥配施秸秆（SNPK）。通过超声波分散-离心分离得到3种粒径土壤——砂粒级（2 000~53 μm）、粉粒级（53~2 μm）和黏粒级（< 2 μm），分析全土及各粒级土壤中全氮和3种土壤氨基糖（氨基葡萄糖、胞壁酸和氨基半乳糖）的含量及变化；基于这3种土壤氨基糖的稳定性和异源性，以氨基糖作为微生物残留物标识物，了解真菌和细菌残留物的积累和转化，阐释真菌和细菌在养分转化中的作用。结果表明：添加有机物料（秸秆、猪圈肥）明显提升了土壤全氮和氨基糖含量，粒级间土壤氮素和氨基糖含量顺序均为黏粒级>砂粒级>粉粒级。添加有机物料对砂粒级土壤氮素影响最大，长期化肥配施猪圈肥中氮素主要在砂粒级中富集，长期化肥配施秸秆的氮素主要在黏粒级中富集。添加秸秆主要提高了真菌来源的氨基葡萄糖的含量，而添加猪圈肥主要提高了土壤中细菌来源的胞壁酸含量，表明添加不同有机物料可影响土壤微生物的群落结构。从各粒级中氨基葡萄糖/胞壁酸的比值来看，添加不同类型外源有机物料对砂粒级土壤微生物群落结构影响最为明显。由此可见，在长期秸秆还田措施下实施有机粪肥部分替代化肥不仅可以减少化肥用量，还可提升土壤养分含量和微生物多样性，改善土壤质量。
Abstract:The North China Plain (NCP) is one of the main grain production bases in China. In pursuance of high yield, excessive application of chemical fertilizers has been becoming increasingly common and problematic, especially in terms of soil quality degradation. A long-term experiment on soil organic nutrient recycling was conducted at Luancheng Agroecosystem Experimental Station, Chinese Academy of Sciences in this study. The aim was to evaluate the effects of different exogenous organic materials application on the distribution of nitrogen and amino sugar in different soil particle-size fractions and to provide the basis for explaining the soil physical and biological protection mechanisms of nitrogen under different management practices. The experiment had six treatments in triplicates-no chemical fertilizer without organic materials (CK), single application of pig manure (M), application of chemical fertilizers (NPK), combined application of chemical fertilizers and pig manure (MNPK), combined application of chemical fertilizers and straw (SNPK) and single application of straws (SCK). Three particle-size fractions (sand:2 000-53 μm, silt:53-2 μm and clay: < 2 μm) were separated through ultrasonic dispersion and centrifugal separation. Total nitrogen and amino sugars (glucosamine, muramic acid and amino galactose) were analyzed within these fractions and in the bulk soil. Based on the stability and heterogeneity of three soil amino sugars, we used amino sugar as index for fungal/bacterial residue accumulation and transformation to explain the role of fungi/bacteria in nutrient conversion. The results indicated that the addition of organic materials (straw and pig manure) significantly increased contents of total nitrogen and amino sugars in bulk soil and different particle-size fractions with order of clay fraction > sand fraction > silt fraction. The effect of adding exogenous organic substances on soil nitrogen content was most obvious in sand fraction. Soil nitrogen in MNPK treatment was enriched mainly in sand fraction, and nitrogen in SNPK was most enriched in clay fraction. Straw addition increased soil content of glucosamine from fungal residue, pig manure application increased content of muramic acid from bacteria residue, indicating obvious effect of exogenous organic substances on community structure of soil microorganisms. The ratios of glucosamine/muramic of soil particle-size fractions showed that bacteria dominated under pig manure application, and fungi dominated under CK or straw addition in nutrient decomposition and transformation, which was most obvious in sand fraction. In summary, partial replacement of chemical fertilizers with organic manure not only reduced chemical fertilizer use, but also increased soil nutrient content, improved microbial community structure and soil quality.
HTML全文
图1不同施肥处理下全土和各粒级土壤中的全氮含量
CK:不施肥无有机物料还田; M:单施猪圈肥; NPK:单施化肥; MNPK:化肥配施猪圈肥; SNPK:化肥配施秸秆; SCK:单施秸秆。不同字母表示P < 0.05水平下差异显著。
Figure1.Total nitrogen contents in bulk soil and different soil particle-size fractions under different long-term fertilization treatments
CK: no fertilizer without organic materials; M: single application of pig manure; NPK: application of chemical fertilizers; MNPK: combined application of chemical fertilizers and pig manure; SNPK: combined application of chemical fertilizers and straw; SCK: single application of straws. Different letters indicate significant differences among different fertilization treatments for the same soil particle-size fraction at P < 0.05.
下载: 全尺寸图片幻灯片
图2不同施肥处理下全土的各种氨基糖含量
CK:不施肥无有机物料还田; M:单施猪圈肥; NPK:单施化肥; MNPK:化肥配施猪圈肥; SNPK:化肥配施秸秆; SCK:单施秸秆。不同字母表示同一粒级不同处理下P < 0.05水平下差异显著。
Figure2.Contents of total and individual amino sugar in bulk soil under different long-term fertilization treatments
CK: no fertilizer without organic materials; M: single application of pig manure; NPK: application of chemical fertilizers; MNPK: combined application of chemical fertilizers and pig manure; SNPK: combined application of chemical fertilizers and straw; SCK: single application of straws. Different letters indicate significant differences among different fertilization treatments at P < 0.05.
下载: 全尺寸图片幻灯片
图3不同施肥处理下土壤氨基葡萄糖与胞壁酸的比值
CK:不施肥无有机物料还田; M:单施猪圈肥; NPK:单施化肥; MNPK:化肥配施猪圈肥; SNPK:化肥配施秸秆; SCK:单施秸秆。不同字母表示同一粒级不同处理下P < 0.05水平下差异显著。
Figure3.Ratio of glucosamine to muramic acid in bulk soil under different long-term fertilization treatments
CK: no fertilizer without organic materials; M: single application of pig manure; NPK: application of chemical fertilizers; MNPK: combined application of chemical fertilizers and pig manure; SNPK: combined application of chemical fertilizers and straw; SCK: single application of straws. Different letters indicate significant differences among different fertilization treatments at P < 0.05.
下载: 全尺寸图片幻灯片
图4不同施肥处理下各粒级土壤中各氨基糖的含量
CK:不施肥无有机物料还田; M:单施猪圈肥; NPK:单施化肥; MNPK:化肥配施猪圈肥; SNPK:化肥配施秸秆; SCK:单施秸秆。不同字母表示同一粒级不同处理在P < 0.05水平下差异显著。
Figure4.Contents of total amino sugar (a), glucosamine (b), galactosamine (c) and muramic acid (d) in different soil particle-size fractions under different long-term fertilization treatments
CK: no fertilizer without organic materials; M: single application of pig manure; NPK: application of chemical fertilizers; MNPK: combined application of chemical fertilizers and pig manure; SNPK: combined application of chemical fertilizers and straw; SCK: single application of straws. Different letters indicate significant differences among different fertilization treatments for the same soil particle-size fraction at P < 0.05.
下载: 全尺寸图片幻灯片
图5不同施肥处理下不同粒级土壤氨基葡萄糖与胞壁酸的比值
CK:不施肥无有机物料还田; M:单施猪圈肥; NPK:单施化肥; MNPK:化肥配施猪圈肥; SNPK:化肥配施秸秆; SCK:单施秸秆。不同小写字母表示同一处理不同粒级间P < 0.05水平下差异显著; 不同大写字母表示同一粒级不同处理间P < 0.05水平下差异显著。
Figure5.Ratio of glucosamine to muramic acid in different soil particle-size fractions under different long-term fertilization treatments
CK: no fertilizer without organic materials; M: single application of pig manure; NPK: application of chemical fertilizers; MNPK: combined application of chemical fertilizers and pig manure; SNPK: combined application of chemical fertilizers and straw; SCK: single application of straws. Different lowercase letters indicate significant differences among different soil particle-size fractions under the same fertilization treatment at P < 0.05. Different capital letters indicate significant differences among different fertilization treatments for the same soil particle-size fraction at P < 0.05.
下载: 全尺寸图片幻灯片
图6不同施肥处理下不同粒级土壤总氨基糖(a)、氨基葡萄糖(b)、氨基半乳糖(c)和胞壁酸(d)的富集因子
CK:不施肥无有机物料还田; M:单施猪圈肥; NPK:单施化肥; MNPK:化肥配施猪圈肥; SNPK:化肥配施秸秆; SCK:单施秸秆。不同字母表示同一粒级不同处理间P < 0.05水平下差异显著。
Figure6.Enrichment factors of total amino sugar (a), glucosamine (b), galactosamine (c) and muramic acid (d) of different soil particle-size fractions under different long-term fertilization treatments
CK: no fertilizer without organic materials; M: single application of pig manure; NPK: application of chemical fertilizers; MNPK: combined application of chemical fertilizers and pig manure; SNPK: combined application of chemical fertilizers and straw; SCK: single application of straws. Different letters indicate significant differences among different fertilization treatments for the same soil particle-size fraction at P < 0.05.
下载: 全尺寸图片幻灯片表1长期不同肥料管理模式下0~20 cm土壤不同粒级颗粒分布
Table1.Distribution of particle-size fractions in 0-20 cm bulk soil under different fertilization treatments
| 施肥处理 Fertilization treatment | 含量Content (%) | 回收率 Recovered rate (%) | ||
| 砂粒 Sand | 粉粒 Silt | 黏粒 Clay | ||
| CK | 14.7±0.4a | 73.4±0.7ab | 11.8±0.3b | 98.8±0.2 |
| M | 13.6±1.1a | 74.5±0.4ab | 11.9±1.4b | 97.6±0.7 |
| NPK | 14.1±2.9a | 75.8±1.5a | 10.0±1.7b | 97.6±0.6 |
| MNPK | 14.2±1.0a | 75.3±0.6a | 10.4±0.5b | 96.4±0.5 |
| SNPK | 14.6±0.4a | 74.3±0.8ab | 11.1±0.6b | 96.2±0.3 |
| SCK | 13.4±0.2a | 71.0±3.3b | 15.6±3.1a | 96.0±0.4 |
| CK:不施肥无有机物料还田; M:单施猪圈肥; NPK:单施化肥; MNPK:化肥配施猪圈肥; SNPK:化肥配施秸秆; SCK:单施秸秆。数据为3次重复的平均值加减标准误。同列不同字母表示P < 0.05水平下差异显著。CK: no fertilizer without organic materials; M: single application of pig manure; NPK: application of chemical fertilizers; MNPK: combined application of chemical fertilizers and pig manure; SNPK: combined application of chemical fertilizers and straw; SCK: single application of straws. Values are means ± S.E (n=3). Different letters within a column indicate significant differences atP < 0.05. | ||||
下载: 导出CSV表2不同施肥处理下各粒级土壤的全氮富集因子
Table2.Enrichment factors of total nitrogen in different particle-size fractions of soil under different long-term fertilization treatments
| 土壤粒级 Soil particle | 施肥处理Fertilization treatment | |||||
| CK | M | NPK | MNPK | SNPK | SCK | |
| 砂粒Sand | 0.8±0.04d | 1.0±0.12d | 1.5±0.15b | 1.8±0.08a | 1.4±0.13bc | 1.2±0.12c |
| 粉粒Silt | 0.8±0.06a | 0.7±0.04a | 0.9±0.15a | 0.8±0.09a | 0.8±0.03a | 0.7±0.02a |
| 黏粒Clay | 3.5±0.12a | 2.9±0.19bc | 3.1±0.08bc | 2.8±0.16c | 3.1±0.12bc | 3.2±0.17ab |
| CK:不施肥无有机物料还田; M:单施猪圈肥; NPK:单施化肥; MNPK:化肥配施猪圈肥; SNPK:化肥配施秸秆; SCK:单施秸秆。数据为3次重复的平均值加减标准误。同行不同字母表示P < 0.05水平下差异显著。CK: no fertilizer without organic materials; M: single application of pig manure; NPK: application of chemical fertilizers; MNPK: combined application of chemical fertilizers and pig manure; SNPK: combined application of chemical fertilizers and straw; SCK: single application of straws. Values are means ± S.E (n=3). Different letters within a column indicate significant differences at P < 0.05. | ||||||
下载: 导出CSV参考文献
| [1] | 王玉英, 李晓欣, 胡春胜, 等.华北平原农田温室气体排放与减排综述[J].中国生态农业学报, 2018, 26(2):167-174 http://www.ecoagri.ac.cn/zgstny/ch/reader/view_abstract.aspx?file_no=2018-0201&flag=1 WANG Y Y, LI X X, HU C S, et al. Review on greenhouse gas emission and reduction in wheat-maize double cropping system in the North China Plain[J]. Chinses Journal of Eco-Agriculture, 2018, 26(2):167-174 http://www.ecoagri.ac.cn/zgstny/ch/reader/view_abstract.aspx?file_no=2018-0201&flag=1 |
| [2] | 张玉铭, 张佳宝, 胡春胜, 等.华北太行山前平原农田土壤水分动态与氮素的淋溶损失[J].土壤学报, 2006, 43(1):17-25 doi: 10.3321/j.issn:0564-3929.2006.01.003 ZHANG Y M, ZHANG J B, HU C S, et al. Nitrogen leaching in wheat-maize rotation field in the North China Plain[J]. Acta Pedologica Sinica, 2006, 43(1):17-25 doi: 10.3321/j.issn:0564-3929.2006.01.003 |
| [3] | 宁川川, 王建武, 蔡昆争.有机肥对土壤肥力和土壤环境质量的影响研究进展[J].生态环境学报, 2016, 25(1):175-181 http://d.old.wanfangdata.com.cn/Periodical/tryhj201601026 NING C C, WANG J W, CAI K Z. The effects of organic fertilizer on soil fertility and soil environmental quality:A review[J]. Ecology and Environment Science, 2016, 25(1):175-181 http://d.old.wanfangdata.com.cn/Periodical/tryhj201601026 |
| [4] | 李燕青, 赵秉强, 李壮.有机无机结合施肥制度研究进展[J].农学学报, 2017, 7(7):22-30 http://d.old.wanfangdata.com.cn/Periodical/zgncxkkj201707005 LI Y Q, ZHAO B Q, LI Z. Research progress of organic-inorganic fertilizer combined application system[J]. Journal of Agriculture, 2017, 7(7):22-30 http://d.old.wanfangdata.com.cn/Periodical/zgncxkkj201707005 |
| [5] | 徐阳春, 沈其荣.长期施用不同有机肥对土壤各粒级复合体中C、N、P含量与分配的影响[J].中国农业科学, 2000, 33(5):1-7 doi: 10.3321/j.issn:0578-1752.2000.05.001 XU Y C, SHEN Q R. Influence of long-term application of manure on the contents and distribution of organic C, total N and P in soil particle sizes[J]. Scientia Agricultura Sinica, 2000, 33(5):1-7 doi: 10.3321/j.issn:0578-1752.2000.05.001 |
| [6] | 崔艳荷, 张威, 何红波, 等.外源氮素添加对森林土壤氨基糖转化的影响[J].生态学杂志, 2016, 35(4):960-965 http://d.old.wanfangdata.com.cn/Periodical/stxzz201604016 CUI Y H, ZHANG W, HE H B, et al. Effect of nitrogen addition on transformation of amino sugars in forest soil[J]. Chinese Journal of Ecology, 2016, 35(4):960-965 http://d.old.wanfangdata.com.cn/Periodical/stxzz201604016 |
| [7] | 王辛辛, 解宏图, 张旭东, 等.底物添加对森林2个不同演替阶段土壤氨基糖动态的影响[J].北京林业大学学报, 2016, 38(4):86-93 http://d.old.wanfangdata.com.cn/Periodical/bjlydxxb201604010 WANG X X, HE H T, ZHANG X D, et al. Effects of substrates on dynamics of amino sugars in two different stages of succession in forest soil[J]. Journal of Beijing Forestry University, 2016, 38(4):86-93 http://d.old.wanfangdata.com.cn/Periodical/bjlydxxb201604010 |
| [8] | 张威, 何红波, 张旭东, 等.东北黑土氨基糖的矿化动态及其对外源物质添加的响应[J].应用生态学报, 2010, 21(10):2593-2598 http://d.old.wanfangdata.com.cn/Periodical/yystxb201010021 ZHANG W, HE H B, ZHANG X D, et al. Amino sugars mineralization and its responses to exogenous substances in black soil of Northeast China[J]. Chinese Journal of Applied Ecology, 2010, 21(10):2593-2598 http://d.old.wanfangdata.com.cn/Periodical/yystxb201010021 |
| [9] | CL MENT P, EDWARD G, JAKOB M, et al. Repeated application of organic waste affects soil organic matter composition:Evidence from thermal analysis, FTIR-PAS, amino sugars and lignin biomarkers[J]. Soil Biology & Biochemistry, 2017, 104, 117-127 https://www.sciencedirect.com/science/article/abs/pii/S0038071716304217 |
| [10] | ZHANG X D, AMELUNG W. Gas chromatographic determination of muramic acid, glucosamine, mannosamine, and galactosamine in soils[J]. Soil Biology and Biochemistry, 1996, 28(9):1201-1206 doi: 10.1016/0038-0717(96)00117-4 |
| [11] | 井艳丽, 刘世荣, 殷有, 等.赤杨对辽东落叶松人工林土壤氨基糖积累的影响[J].生态学报, 2018, 38(8):2838-2845 http://d.old.wanfangdata.com.cn/Periodical/stxb201808021 JING Y L, LIU S R, YIN Y, et al. Effect of N-mixing tree species (Alnus sibirica) on amino sugars in the soils of a Larix kaempferi plantation in eastern Liaoning Province, China[J]. Acta Ecologica Sinica, 2018, 38(8):2838-2845 http://d.old.wanfangdata.com.cn/Periodical/stxb201808021 |
| [12] | 何红波, 张威, 解宏图, 等.测定土壤氨基糖和氨基酸手性异构体中氮同位素比值的气相色谱/质谱方法[J].土壤学报, 2009, 46(2):289-298 doi: 10.3321/j.issn:0564-3929.2009.02.014 HE H B, ZHANG W, XIE H T, et al. A GC/MS method to assess 15N ratios in soil amino sugars and amino acid enantiomers[J]. Acta Pedologica Sinica, 2009, 46(2):289-298 doi: 10.3321/j.issn:0564-3929.2009.02.014 |
| [13] | 胡国庆, 刘肖, 何红波, 等.黄河三角洲不同盐渍化土壤中氨基糖的积累特征[J].土壤学报, 2018, 55(2):390-398 http://d.old.wanfangdata.com.cn/Periodical/trxb201802013 HU G Q, LIU X, HE H B, et al. Accumulation characteristics of amino sugars in salinized soil of different types in the Yellow River Delta[J]. Acta Pedological Sinica, 2018, 55(2):390-398 http://d.old.wanfangdata.com.cn/Periodical/trxb201802013 |
| [14] | ENGELKING B, FLESSA H, JOERGENSEN R G. Shifts in amino sugar and ergosterol contents after addition of sucrose and cellulose to soil[J]. Soil Biology & Biochemistry, 2007, 39(8):2111-2118 https://www.sciencedirect.com/science/article/abs/pii/S0038071707001228 |
| [15] | LIANG C, DUNCAN D S, BALSER T C, et al. Soil microbial residue storage linked to soil legacy under biofuel cropping systems in southern Wisconsin, USA[J]. Soil Biology & Biochemistry, 2013, 57:939-942 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=d8f6fe6fdfa6512aacd0aa5887a10e79 |
| [16] | 吕慧捷.肥料氮向土壤有机组分转化及稳定机制研究[D].北京: 中国科学院大学, 2012: 6-9 LYU H J. The seasonal dynamics and stabilization of fertilizer-derived N transformed into different soil organic N fractions[D]. Beijing: University of Chinese Academy of Sciences, 2012: 6-9 |
| [17] | CHRISTENSEN B T. Physical fractionation of soil and structural and functional complexity in organic matter turnover[J]. European Journal of Soil Science, 2001, 52(3):345-353 doi: 10.1046/j.1365-2389.2001.00417.x |
| [18] | JONES D L, EDWARDS A C. Influence of sorption on the biological utilization of two simple carbon substrates[J]. Soil Biology & Biochemistry, 1998, 30(14):1895-1902 https://www.sciencedirect.com/science/article/abs/pii/S0038071798000601 |
| [19] | OADES J M. The retention of organic matter in soils[J]. Biochemistry, 1988, 5(1):35-70 http://d.old.wanfangdata.com.cn/OAPaper/oai_doaj-articles_6bc7a8015cd34362942a907e7c2e7635 |
| [20] | PARFITT R L, THENG B K, WHITTON J S, et al. Effects of clay minerals and land use on organic matter pools[J]. Geoderma, 1997, 75(1):1-12 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=0762050f0510e70732d974302bd98857 |
| [21] | 胡景恒, 朴河春, 刘启明.碳水化合物在土壤中的分布特征及其环境意义[J].地质地球化学, 2000, 28(2):59-64 http://d.old.wanfangdata.com.cn/Periodical/dzdqhx200003007 HU J H, PIAO H C, LIU Q M. The distribution and environmental effect of carbohydrate in soil[J]. Geology-Geochemistry, 2000, 28(2):59-64 http://d.old.wanfangdata.com.cn/Periodical/dzdqhx200003007 |
| [22] | 刘秀梅, 冯兆滨, 张树清, 等.纳米-亚微米级复合材料对褐潮土有机无机复合体含量及各粒级复合体中C、N、P含量与分布的影响[J].植物营养与肥料学报, 2007, 13(1):57-63 doi: 10.3321/j.issn:1008-505X.2007.01.010 LIU X M, FENG Z B, ZHANG S Q, et al. Effect of nano-subnanocomposites on contents and distribution of organic C, total N and P in soil organic-mineral granules in drab fluvo-aquic soil[J]. Plant Nutrition and Fertilizer Science, 2007, 13(1):57-63 doi: 10.3321/j.issn:1008-505X.2007.01.010 |
| [23] | 卢瑛, 甘海华, 徐盛荣.不同肥力红壤有机无机复合体的氮素特征与供应性能[J].华南农业大学学报, 1995, 16(4):64-68 http://www.cnki.com.cn/Article/CJFDTOTAL-HNNB504.014.htm LU Y, GAN H H, XU S R. Nitrogen characteristic and supplying property of organic-mineral complexes of different fertility red soils[J]. Journal of South China Agricultural University, 1995, 16(4):64-68 http://www.cnki.com.cn/Article/CJFDTOTAL-HNNB504.014.htm |
| [24] | 吕慧捷, 何红波, 张旭东.土壤颗粒分级过程中超声破碎和离心分离的条件选择[J].土壤通报, 2012, 43(5):1126-1130 http://www.cnki.com.cn/Article/CJFDTOTAL-TRTB201205017.htm LYU H J, HE H B, ZHANG X D. The options of conditions on ultrasonic dispersion and centrifugal separation in soil particle size fractionation[J]. Chinese Journal of Soil Science, 2012, 43(5):1126-1130 http://www.cnki.com.cn/Article/CJFDTOTAL-TRTB201205017.htm |
| [25] | ANDERSON D W, SAGAR S, BETTANY J R, et al. Particle size fractions and their use in studies of soil organic matter. 1. The nature and distribution of forms of carbon, nitrogen and sulfur[J]. Soil Science Society of America, 1981, 45(4):767-772 doi: 10.2136/sssaj1981.03615995004500040018x |
| [26] | CHRISTENSEN B T. Carbon and nitrogen in particle size fractions isolated from Danish arable soils by ultrasonic dispersion and gravity sedimentation[J]. Acta Agriculturae Scandinavica, 1985, 35(2):175-187 doi: 10.1080/00015128509435773 |
| [27] | SHANG C, TJESSEN H. Organic matter stabilization in two semi-arid tropical soils:Size, density, and magnetic separations[J]. Soil Science Society of America, 1998, 62(5):1247-1257 doi: 10.2136/sssaj1998.03615995006200050015x |
| [28] | 公华锐, 李静, 马军花, 等.秸秆还田配施有机无机肥料对冬小麦土壤水氮变化及其微生物群落和活性的影响[J].生态学报, 2019, DOI: 10.5846/stxb201803190537 GONG H R, LI J, MA J H, et al. Effects of straw incorporation combined with inorganic-organic fertilization on soil water and nitrogen changes and microbial community structure in winter wheat[J]. Acta Ecologica Sinica, 2019, DOI: 10.5846/stxb201803190537 |
| [29] | 姚晓东, 王娓, 曾辉.磷脂脂肪酸法在土壤微生物群落分析中的应用[J].微生物学通报, 2016, 43(9):2086-2095 http://d.old.wanfangdata.com.cn/Periodical/wswxtb201609025 YAO X D, WANG W, ZENG H. Application of phospholipid fatty acid method in analyzing soil microbial community composition[J]. Microbiology China, 2016, 43(9):2086-2095 http://d.old.wanfangdata.com.cn/Periodical/wswxtb201609025 |
| [30] | 席劲瑛, 胡洪营, 钱易. Biology方法在环境微生物群落研究中的应用[J].微生物学报, 2003, 43(1):138-141 doi: 10.3321/j.issn:0001-6209.2003.01.022 XI J Y, HU H Y, QIAN Y. Application of biology system in the study of microbial community[J]. Acta Microbiologica Sinica, 2003, 43(1):138-141 doi: 10.3321/j.issn:0001-6209.2003.01.022 |
| [31] | 苏淑芳, 于清军, 刘亚军, 等.秸秆覆盖免耕对土壤氨基糖在团聚体粒级中分布的影响[J].土壤通报, 2017, 48(2):365-371 http://d.old.wanfangdata.com.cn/Periodical/trtb201702016 SU S F, YU Q J, LIU Y J, et al. Effects of no-tillage with stalk mulching on distribution of amino sugars in soil aggregate fractions[J]. Chinses Journal of Soil Science, 2017, 48(2):365-371 http://d.old.wanfangdata.com.cn/Periodical/trtb201702016 |
| [32] | DING X L, LIANG C, ZHANG B, et al. Higher rates of manure application lead to greater accumulation of both fungal and bacterial residues in macroaggregates of a clay soil[J]. Soil Biology & Biochemistry, 2015, 84:137-146 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=364223864e8925e26cee5947fa0be407 |
| [33] | 闫颖.长期施肥对土壤矿物粒级中碳水化合物分布特征的影响[D].北京: 中国科学院大学, 2007 YAN Y. Carbohydrate accumulation and distribution in particle-size fractions of two soils as affected by long-term fertilizations[D]. Beijing: University of Chinese Academy of Sciences, 2007 |
| [34] | 丁雪丽.秸秆氮素合成氨基糖的微生物过程及其同位素区分[D].北京: 中国科学院大学, 2009 DING X L. Microbial synthesis process of amino sugars from 15N-labeled maize residues[D]. Beijing: University of Chinese Academy of Sciences, 2009 |
| [35] | 王克鹏, 张仁陟, 谢军红, 等.长期免耕和秸秆覆盖下黄土高原旱作土壤不同粒级复合体中酸解有机氮含量及分配比例变化[J].植物营养与肥料学报, 2016, 22(3):659-666 http://d.old.wanfangdata.com.cn/Periodical/zwyyyflxb201603010 WANG K P, ZHANG R Z, XIE J H, et al. Acid hydrolysis organic N content and the distribution in different sizes of soil complexes in the Loess Plateau dryland under long-term no-tillage and straw mulching[J]. Journal of Plant Nutrition and Fertilizer, 2016, 22(3):659-666 http://d.old.wanfangdata.com.cn/Periodical/zwyyyflxb201603010 |
| [36] | 王岩, 徐阳春, 沈其荣.有机、无机肥料15N在土壤不同粒级中的分布及其生物有效性[J].土壤通报, 2002, 33(6):410-413 doi: 10.3321/j.issn:0564-3945.2002.06.003 WANG Y, XU Y C, SHEN Q R. Distribution of 15N from organic and inorganic fertilizers in different size fractions of soil and its availability[J]. Chinese Journal of Soil Science, 2002, 33(6):410-413 doi: 10.3321/j.issn:0564-3945.2002.06.003 |
| [37] | 丁雪丽, 何红波, 张旭东, 等.无机氮素加入量对玉米秸秆分解过程中棕壤氨基糖含量的影响[J].土壤学报, 2011, 48(3):665-671 http://d.old.wanfangdata.com.cn/Periodical/trxb201103028 DING X L, HE H B, ZHANG X D, et al. Effects of inorganic nitrogen application rate on content of amino sugars in alfisol during microbial decomposition of corn stalks[J]. Acta Pedological Sinica, 2011, 48(3):665-671 http://d.old.wanfangdata.com.cn/Periodical/trxb201103028 |
| [38] | 丁雪丽, 何红波, 张旭东, 等.不同供氮水平对施用玉米秸秆后黑土氨基糖转化的影响[J].应用生态学报, 2009, 20(9):2207-2213 http://d.old.wanfangdata.com.cn/Periodical/yystxb200909024 DING X L, HE H B, ZHANG X D, et al. Effects of nitrogen supply level on microbial transformation of amino sugar in a mollisol amended with maize straw[J]. Chinese Journal of Applied Ecology, 2009, 20(9):2207-2213 http://d.old.wanfangdata.com.cn/Periodical/yystxb200909024 |
| [39] | 申小冉, 徐明岗, 张文菊, 等.长期不同施肥对土壤各粒级组分中氮含量及分配比例的影响[J].植物营养与肥料学报, 2012, 18(5):1127-1134 http://www.cnki.com.cn/Article/CJFDTotal-ZWYF201205012.htm SHEN X R, XU M G, ZHANG W J, et al. Effect of various long-term fertilization on soil nitrogen concentration and distribution percentage in particle-size fractions[J]. Plant Nutrition and Fertilizer Science, 2012, 18(5):1127-1134 http://www.cnki.com.cn/Article/CJFDTotal-ZWYF201205012.htm |
| [40] | 闫颖, 何红波, 张旭东, 等.有机肥对棕壤不同粒级有机碳和氮的影响[J].土壤通报, 2008, 39(4):738-742 doi: 10.3321/j.issn:0564-3945.2008.04.005 YAN Y, HE H B, ZHANG X D, et al. Effect of manure application on the organic C and N in brown earth and particle-size fractiona[J]. Chinese Journal of Soil Science, 2008, 39(4):738-742 doi: 10.3321/j.issn:0564-3945.2008.04.005 |
| [41] | HASSINK J. The capacity of soils to preserve organic C and N by their association with clay and silt particles[J]. Plant and Soil, 1997, 191(1):77-87 doi: 10.1023/A:1004213929699 |
| [42] | CARTER M, ANGERS D A, GREGORICH E G, et al. Characterizing organic matter retention for surface soils in eastern Canada using density and particle size fractions[J]. Canadian Journal of Soil Science, 2003, 83(1):11-23 doi: 10.4141/S01-087 |
| [43] | 詹雨珊, 冯有智.秸秆还田对水稻土微生物影响的研究进展[J].土壤通报, 2017, 48(6):1530-1536 http://d.old.wanfangdata.com.cn/Periodical/trtb201706036 ZHAN Y S, FENG Y Z. Advances on impact of straw returning on microorganisms in paddy soil[J]. Soil Science, 2017, 48(6):1530-1536 http://d.old.wanfangdata.com.cn/Periodical/trtb201706036 |
| [44] | CHOL G L, TAKESHI W, YUTAKA S, et al. Bacterial populations assimilating carbon from 13C-labeled plant residue in soil:Analysis by a DNA-SIP approach[J]. Soil Biology & Biochemistry, 2011, 43(4):81-822 https://www.sciencedirect.com/science/article/abs/pii/S0038071710004852 |
| [45] | 孙悦, 徐兴良, KUZYAKOV Y.根际激发效应的发生机制及其生态重要性[J].植物生态学报, 2014, 38(1):62-75 http://d.old.wanfangdata.com.cn/Periodical/zwstxb201401007 SUN Y, XU X L, KUZYAKOV Y. Mechanisms of rhizosphere priming effects and their ecological significance[J]. Chinese Journal of Plant Ecology, 2014, 38(1):62-75 http://d.old.wanfangdata.com.cn/Periodical/zwstxb201401007 |
| [46] | 徐阳春, 沈其荣.有机肥和化肥长期配合施用对土壤及不同粒级供氮特性的影响[J].土壤学报, 2004, 41(1):87-92 doi: 10.3321/j.issn:0564-3929.2004.01.014 XU Y C, SHEN Q R. Influence of long-term combined application of manure and chemical fertilizer on supplying characteristics of nitrogen in soil and soil particle fractions[J]. Acta Pedologica Sinica, 2004, 41(1):87-92 doi: 10.3321/j.issn:0564-3929.2004.01.014 |
