Identification of the Cysteine Protease Family and Corresponding miRNAs in Jatropha curcas and Their Response to Chill-hardening
Dandan Wu1, Yongkun Chen1,2, Yu Yang1, Chunyan Kong1, Ming Gong,1,*1Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, School of Life Sciences, Yunnan Normal University, Kunming 650500, China 2School of Life Sciences, Xinjiang Normal University, Urumqi 830054, China
Abstract Jatropha curcas is a kind of promising energy plants, but also a chilling-sensitive plant, which can be chill- hardening at 12°C with significant improvement of its chilling tolerance. In this study, the cysteine protease gene family of J. curcas and their corresponding miRNAs were identified at the genome-wide level. The results showed that a total of 39 cysteine protease genes were identified in J. curcas genome, which were located on 11 chromosomes and could be divided into six subfamilies (C1A, C2, C12, C13, C14 and C15); all encoding 181-2 158 amino acids with Cys and His active sites. Based on the sequencing results of miRNAome and degradome, 283 miRNAs were found to be targeted to 14 members of cysteine protease gene family. In addition, the co-expression analysis of those miRNAs targeting to JcDEK1, JcRD21B and JcXBCP3L during chill-hardening demonstrated significantly negative correlation during the chill-hardening at 12°C, suggesting that these miRNAs are involved in the regulation of the cysteine protease genes, and this regulation should be related to the enhancement of chilling tolerance induced by the chill-hardening. This study will be helpful for better understanding the function of cysteine protease gene family in J. curcas and the interaction of the family genes with their corresponding miRNAs, and how this interaction regulates the response of J. curcas to low temperature. Keywords：Jatropha curcas;cysteine protease;gene family;microRNAs;low temperature response
PDF (2328KB)元数据多维度评价相关文章导出EndNote|Ris|Bibtex收藏本文 引用本文 吴丹丹, 陈永坤, 杨宇, 孔春艳, 龚明. 小桐子半胱氨酸蛋白酶家族和相应miRNAs的鉴定及其对低温锻炼的响应. 植物学报, 2021, 56(5): 544-558 doi:10.11983/CBB21014 Wu Dandan, Chen Yongkun, Yang Yu, Kong Chunyan, Gong Ming. Identification of the Cysteine Protease Family and Corresponding miRNAs in Jatropha curcas and Their Response to Chill-hardening. Chinese Bulletin of Botany, 2021, 56(5): 544-558 doi:10.11983/CBB21014
红色代表半胱氨酸蛋白酶基因, 蓝色代表miRNAs。 Figure 7Networks of targeted regulation among miRNAs and their corresponding cysteine protease family genes in Jatropha curcas
Red represents cysteine protease gene and blue represents miRNAs.
*与**分别表示不同时间的表达量与对照0小时在P<0.05与P<0.01水平差异显著。 Figure 9Co-expression analysis by qRT-PCR of the cysteine protease genes JcDEK1, JcRD21B and JcXBCP3L as well as the miRNAs targeting these genes in Jatropha curcas seedlings during chill-hardening at 12°C
* and ** indicated the significant differences between the expression level at different time and control 0 h at P<0.05 and P<0.01 level, respectively.
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miRNA作用机制研究进展 2 2020
... miRNA是一类真核生物中广泛存在的长度为20-24 nt的内源非编码RNA, 可能的作用机制主要包括转录本切割、翻译抑制、次生siRNA形成、翻译激活、转录增强和类激素作用6个方面(郝大海和龚明, 2020).植物miRNA主要通过剪切mRNA或抑制翻译负调控基因表达, 在细胞增殖分化、个体生长发育和抵御逆境胁迫等生理过程中发挥重要作用(张翠桔等, 2020; 王劲东等, 2020).许多研究表明, miRNAs参与植物对低温的响应、信号转导和适应过程(Megha et al., 2018), 但miRNAs是否参与调控半胱氨酸蛋白酶家族基因, 以及这种互作如何调控植物耐冷性的研究尚未见报道.基于本实验室前期的工作(Ao et al., 2013a, 2013b; Wang et al., 2013a, 2013b; Li et al., 2014)和近期完成的小桐子低温锻炼过程中转录组、miRNA组和降解组测序结果, 我们对小桐子半胱氨酸蛋白酶家族进行了全基因组鉴定及系统进化、染色体定位、蛋白结构域及基因表达分析; 并基于miRNA组和降解组测序结果, 鉴定了靶向半胱氨酸蛋白酶基因家族成员的miRNAs, 进行共表达分析, 旨在阐明半胱氨酸蛋白酶基因家族及其与对应miRNAs的互作关系以及这种互作如何参与调控小桐子对低温锻炼的响应过程. ...
,1,*
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