关键词:棉花; 真实性鉴定; 纯度检测; SSR分子标记 Rapid Identification System of Purity and Authenticity in Cotton Varieties by SSR Markers WANG Xin-Yi1, AI Xian-Tao2, WANG Jun-Duo2, LIANG Ya-Jun2, GONG Zhao-Long2, ZHENG Ju-Yun2, GUO Jiang-Ping2, MAMAT Mo-Ming2, LI Xue-Yuan2,* 1 Key Laboratory of Agriculture Biological Technology / College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China
2 Economic Crop Research Institute, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
Fund:The study was supported by the National Natural Science Foundation of China (31360351). AbstractSSR molecular markers were used to analyze the authenticity and purity of cotton varieties, providing a molecular basis for the identification of cotton varieties. Typical cotton varieties were used as materials, and 26 pairs of primers well-distributed on the cotton genome were ascertained as the core primers in SSR molecular identification by preliminary and multiple screening. A total of 138 alleles from 120 varieties were screened by using 26 pairs of core primers, 129 of which were polymorphic loci, with a polymorphism rate of 93.48%. Ten inbred cotton varieties were used with 26 pairs of SSR core primers to identify the variety purity. The DNA fingerprint of one variety (Yuanmian 6) shared high uniformity with that of the standard cultivar, and DNA fingerprints of other nine varieties had 8-18 loci different from those of standard cultivar. The results from SSR molecular marker matched with those from field experiments. Five specific primers were selected for testing authenticity of each cultivar from 26 core primers. The variety with highest purity was Yuanmian 6, with the purity of 98.0% and that with lowest purity was Yuanmian 1, with the purity of 90.5%. It suggests that identifying authenticity and purity of cotton varieties is feasible by SSR molecular markers.
Keyword:Cotton; Authenticity identification; Purity testing; SSR marker Show Figures Show Figures
图1 A和B不同比例DNA混合样本标记扩增结果 M: marker; 1~11: 2个品种DNA按10:0、9:1、8:2、7:3、6:4、5:5、4:6、3:7、2:8、1:9、0:10比例混合的11个样品。Fig. 1 SSR bands of mixed DNA samples from Xinluzao 26 and Xinluzhong 37 M: marker; 1-11: DNA samples of two varieties mixed by the ratio of 10:0, 9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8, 1:9, 0:10.
Bt+CpTI双价抗虫基因, 通过花粉管通道法导入中棉所23中, 经过多年选育而成 Bt+CpTI Bivalent insect-resistant gene was introduced into Zhongmiansuo 23 by pollen tube path, by several years breeding
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中棉所43 Zhongmiansuo 43
石远321× 5716 Shiyuan 321× 5716
表2 用于真实性鉴定的10个常规棉花品种 Table 2 List of 10 inbred cotton varieties for authenticity identification
图2 引物NAU2083在不同品种3组混合样本中的扩增结果M: marker; 1~30: 不同品种混合样本电泳扩增效果图; A~J: 10个参试品种。Fig. 2 Amplified bands of three mixed samples by the different varieties with primer NAU2083 M: marker; 1-30: electrophorogram of mixed samples of different varieties; A-J: 10 tested varieties.
图3 引物NAU2083在15个单株(新陆早13和新陆中37)的扩增结果 M: marker; a: 新陆早13的电泳扩增图; b: 新陆中37的电泳扩增图。Fig. 3 Amplified result of 15 single plants (Xinluzao 13 and Xinluzhong 37) with primer NAU2083 M: marker; a: electrophorogram of Xinluzao 13; b: electrophorogram of Xinluzhong 37.
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