关键词:棉花; 低温; 光抑制; 光合作用; 环式电子传递流 Effects of Low Temperature on PSI and PSII Photoinhibition in Cotton Leaf at Boll Stage XIAO Fei1, YANG Yan-Long2, WANG Ya-Ting2, MA Hui2, ZHANG Wang-Feng2,* 1College of Life Science, Shihezi University, Shihezi 832003, China
2 College of Agriculture, Shihezi University / Key Laboratory of Oasis Ecology Agriculture of Xinjiang Production and Construction Corps, Shihezi 832003, China
Fund:This study was supported by the National Natural Science Foundation of China (U1203283) AbstractCotton ( Gossypium hirsutum L.) variety Xinluzao 45 was grown in pots under low temperature until bolling stage and the seedings were moved in phytotron in northern Xinjiang. Chl fluorescence and P700+ absorbance signal were determined simultaneously by Dual-PAM-100. The treatment was day/night temperature of 16°C/10°C with a suitable temperature condition (30°C/18°C) as control. The light-adapted maximum quantum yield of PSII ( Fv°/ Fm°), photochemical quenching coefficient ( qP) and effective quantum yield of PSII [Y(II)] decreased significantly under low temperature stress. Low temperature significantly increased non-photochemical quantum yield of PSI caused by donor side limitation [Y(ND)]. The yield of regulated energy dissipation [Y(NPQ)] and non-regulated energy dissipation of PSII [Y(NO)] were significantly increased, including reversible photoinhibition in cotton leaf. Compared with control, low temperature stress significantly decreased the acceptor side limitation of PSI [Y(NA)] and increased donor side limitation of PSI [Y(ND)], while effective PSI complex content ( Pm) was not significantly decreased, suggesting that PSI in cotton leaf is insensitive to low temperature compared with PSII. The quantum yield of cyclic electron flow [Y(CEF)] and the ratio of [Y(CEF)] to the effective quantum yield of PSII [Y(CEF)/Y(II)] were enhanced by low temperature stress in cotton suggesting that stimulation of cyclic electron flow plays an important role in protecting PSI and PSII from photoinhibition caused by low temperature stress in cotton. Furthermore, the non-photochemical quenching (NPQ) and regulated heat dissipation [Y(NPQ)] had significantly positive correlation with the quantum yield of cyclic electron flow [Y(CEF)], indicating that the strong excess excitation energy due to the overclosure of PSII reaction center results in reversible photoinhibition of PSII under low temperature stress. In conclusion, the strong stimulation of cyclic electron flow and regulated heat dissipation powerfully prevent PSII and PSI of cotton from photoinhibition and photodamage induced by low temperature stress, which may be the main mechanism of the insusceptibility of PSI in cotton to low temperature stress.
Keyword:Cotton; Low temperature; Photoinhibition; Photosynthesis; Cyclic electron transport flow Show Figures Show Figures
图1 低温对棉花花铃期叶片PSII最大光化学量子产量(Fv/Fm)和最大光氧化态P700含量(Pm)的影响R1、R3和R5分别表示恢复后1、3和5 d。不同小写字母表示不同处理时间存在显著差异(P < 0.05)。Fig. 1 Changes in the maximum quantum yield of PSII after dark adaption (Fv/Fm) and maximum photo-oxidiable P700 contents (Pm) in cotton leaf at boll stage under low temperatureR1, R3, and R5 refer to 1, 3, and 5 days after recovery. The different letters indicate significance at P < 0.05 between different treatment time.
图3 低温对棉花花铃期叶片Y(II)、Y(NPQ)、Y(NO)和ETRPSII的影响Y(II): PSII有效光化学量子产量; Y(NPQ): 调节性热耗散; Y(NO): 非调节性热耗散; ETRPSII: PSII电子传递速率。Fig. 3 Effects of low temperature on Y(II), Y(NPQ), Y(NO), and ETRPSII in cotton leaf at boll stageY(II): effective photochemical quantum yield of photosystem II; Y(NPQ): regulated heat dissipation; Y(NO): non-regulated heat dissipation; ETRPSII: electron transport rate of photosystem II.
图4 低温对棉花花铃期叶片光系统I (PSI)氧化还原状态的影响Y(I): PSI有效光化学量子产值; Y(ND): 全部P700在给定的光照下被氧化的比例, 表示PSI由于供体侧限制引起的PSI非光化学量子产量; Y(NA):全部的P700在给定的状态下由于缺乏电子受体难以被饱和光氧化的比例, 即由于受体侧限制引起的PSI非光化学量子产量。Fig. 4 Effects of low temperature on the redox state of P700 in cotton leaf at boll stageY(I): effective photochemical quantum yield of PSI; Y(ND): fraction of overall P700 that is oxidized in a given state, i.e. the quantum yield of non-photochemial energy dissipation due to donor side limitation; Y(NA): fraction of overall P700 that cannot be oxidized in a given state due to reduced PSI electron acceptors, i.e. the quantum yield of non-photochemical energy dissipation due to acceptor side limitation.
图6 棉花经低温处理后环式电子传递量子产值[Y(CEF)]分别与非光化学热耗散(NPQ) (B)、PSI供体侧限制[Y(ND)] (C)和调节性能量耗散[Y(NPQ)] (D)间的相关性Fig. 6 Correlation of the quantum yield of cyclic electron flow [Y(CEF)] with non-photochemical quenching (NPQ) (B), donor side limitation of PSI[Y(ND)] (C), and regulated heat dissipation [Y(NPQ)] (D), respectively, in cotton under low temperature
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