关键词:彩色棉; 纤维发育; 色素合成; 植物生长调节剂 Effects of Plant Growth Regulators on Fiber Growth and Development in Colored Cotton Ovule Culture in vitro ZHANG Xiao-Meng**, LIU Song-Jiang**, GONG Wen-Fang, SUN Jun-Ling, PANG Bao-Yin, DU Xiong-Ming* Institute of Cotton Research of Chinese Academy of Agricultural Sciences / State Key Laboratory of Cotton Biology, Anyang 455000, China Fund:This study was supported by the National Natural Science Foundation of China (31601353) AbstractNaturally colored cotton is an ideal textile raw material for the environmental protection and human health. The ovule of cotton varieties Z1-61, Lyumian CC28, and RT-baixu (CK) was cultured in the media with different concentrations of plant growth regulators (MeJA, SHAM, BR, BRz, FLD, ETH, CoCl2, PAL inhibitor, 4CL inhibitor, Urea, and Chl). After 30 days, the fiber color, fiber length, ovule fresh weight, ovule dry weight and fiber dry weight were determined and analyzed with Duncan's new multiple range method. Under the treatments of salicylhydroxamic, phenylalanine ammonia solution enzyme inhibitor and 4CL inhibitor, the color of Z1-61 and Lyumian CC28 cotton fiber was light, and the higher the concentration of the Salicylhydroxamic acid, the lighter of the cotton fiber color. BRz (2.5 μmol L-1) was conducive to the pigment accumulation in brown cotton fiber, while did not have any significant effect on the pigment appearance of green cotton. Both FLD and CoCl2 made the color of brown cotton fiber lighter, while CoCl2 had little influence on the green color cotton fiber and inhibited the formation of callus. Moreover, salicylhydroxamic acid, BRz, FLD, CoCl2, phenylalanine ammonia solution enzyme inhibitor or 4CL inhibitor decreased the fiber length, ovule fresh weight, fiber dry weight and ovule dry weight. In addition, 5 g L-1 urea or 1 mg L-1 Chl were advantageous to the brown cotton fiber pigment synthesis. In conclusion, the growth regulators tested in this study revealed the correlations among pigments synthesis, hormone, flavonoid metabolism and protein metabolism, which would be useful for developing colored cotton cultivars.
Keyword:Colored cotton; Fiber growth; Pigment synthesis; Plant growth regulators Show Figures Show Figures
4 结论茉莉酸甲酯抑制剂从反向印证了茉莉酸甲酯在棕色棉纤维伸长和色素合成过程中的关键作用; 油菜素内酯抑制剂也从反向揭示出BR在棉胚珠发育和纤维伸长中的作用; 脱落酸合成抑制剂和乙烯抑制剂处理均对胚珠和纤维发育不利, 从反向证明脱落酸和乙烯可能对纤维次生代谢物质合成有促进作用; 苯丙氨酸解氨酶(PAL)专一性抑制剂和4-香豆酸辅酶A连接酶(4CL)抑制剂的添加显著抑制棕色棉和绿色棉纤维发育和色素合成, 证实了彩色棉色素成分中黄酮类物质的存在; 适宜浓度蛋白质含量促进彩色棉纤维伸长和色素合成。 The authors have declared that no competing interests exist.
董合忠, 李维江, 唐薇, 张冬梅. 彩色棉纤维发育与色素形成. , 2004, 31(2): 2-4Dong HZ, Li WJ, TangW, Zhang DM. Pigment synthesis and cotton fiber development of color cotton. , 2004, 31(2): 2-4 (in Chinese)[本文引用:1]
[2]
詹少华, 林毅, 蔡永萍, 文汉. 天然棕色棉色素分布规律及色素合成与纤维发育的关系. , 2006, 18(3): 170-174Zhan SH, LinY, Cai YP, WenH. The pigment distribution regulation of the natural brown cotton and relations between pigment synthesis and cotton fiber development. , 2006, 18(3): 170-174 (in Chinese with English abstract)[本文引用:1]
[3]
詹少华, 林毅, 蔡永萍, 李正鹏. 天然棕色棉纤维色素光谱学特性及其化学结构初步推断. , 2007, 24: 99-104Zhan SH, LinY, Cai YP, Li ZP. Preliminary deductions of the chemical structure of the pigment brown in cotton fiber. , 2007, 24: 99-104 (in Chinese with English abstract)[本文引用:1]
[4]
RyserU, MeierH, Holloway PJ. Identification and localization of suberin in the cell walls of green cotton fibres (Gossypium hirsutum L. , var. green lint). , 1983, 117: 196-205[本文引用:1]
[5]
胡超, 杨园园, 郭宁, 蔡永萍, 林毅, 高俊山, 姜家生. 棕色棉与白色棉缩合单宁单体儿茶素动态变化的比较. , 2011, 47: 685-690HuC, Yang YY, GuoN, Cai YP, LinY, Gao JS, Jiang JS. Comparison of dynamic changes in condensed tannin monomer catechins between brown cotton and white cotton. , 2011, 47: 685-690 (in Chinese with English abstract)[本文引用:1]
[6]
赵向前, 王学德. 天然彩色棉纤维色素成分的研究. , 2005, 31: 456-462Zhao XQ, Wang XD. Composition analysis of pigment in colored cotton fiber. , 2005, 31: 456-462 (in Chinese with English abstract)[本文引用:1]
[7]
张美玲. 彩色棉纤维分化发育规律与色素成分研究. , 2013Zhang ML. Exploration of Fiber Differentiation and Development and Pigment Component in Colored Cotton. , 2013 (in Chinese with English abstract)[本文引用:2]
[8]
SchmutzA, Buchala AJ, RyserU. Changing the dimensions of suberin lamellae of green. fibers with a specific inhibitor of the endoplasmic reticulum-associated fatty acid elongases. , 1996, 110: 403-411[本文引用:1]
[9]
汪淼, 陈沙沙, 蔡永萍, 林毅, 孙旭, 郭宁, 高俊山, 姜家生. 光质对棕色棉纤维色素形成的影响. , 2013, 25: 329-333WangM, Chen SS, Cai YP, LinY, SunX, GuoN, Gao JS, Jiang JS. Effect of light quality on pigment formation in brown cotton fiber. , 2013, 25: 329-333 (in Chinese with English abstract)[本文引用:1]
[10]
Gong WF, He SP, Tian JH, Sun JL, Pan ZE, Jia YH, Sun GF, Du XM. Comparison of the transcriptome between two cotton lines of different fiber color and quality. , 2014, 9: e112966[本文引用:1]
[11]
Waghmare VN, Koranne KD. The present situation, problems and future potential of the colored cotton cultivation. , 2000, 19: 6[本文引用:1]
[12]
王学德, 李悦有. 彩色棉纤维发育的特性研究. , 2002, 28: 237-242Wang XD, Li YY. Study on characteristics of colored cotton fiber development. , 2002, 28: 237-242 (in Chinese with English abstract)[本文引用:1]
[13]
潘兆娥, 杜雄明, 孙君灵, 周忠丽, 庞保印. 遮光对彩色棉的色泽及纤维品质的影响. , 2006, 18: 264-268Pan ZE, Du XM, Sun JL, Zhou ZL, Pang BY. Influences of boll shading on fiber color and fiber quality of colored cotton. , 2006, 18: 264-268 (in Chinese with English abstract)[本文引用:1]
[14]
袁淑娜. 彩色棉纤维发育特性以及基于细胞质雄性不育的彩色长绒棉育种研究. , 2009Yuan SN. Studies on Characteristics of Colored Cotton Fiber Development and Breeding for Colored Cotton with Long Fiber Based on Cytoplasmic Male Sterility System. , 2009 (in Chinese with English abstract)[本文引用:1]
[15]
Yuan SN, MalikW, Hua SJ, BibiN, Wang X D. in vitro inhibition of pigmentation and fiber development in colored cotton. , 2012, 13: 478-486[本文引用:1]
[16]
Beasly CA, Ting IP. The effects of plant growth substances on in vitro fiber development from fertilized cotton ovules. , 1973, 60: 130-l39[本文引用:2]
[17]
Shi YH, Zhu SW, Mao XZ, Feng JX, Qin YM, ZhangL, ChengJ, Wei LP, Wang ZY, Zhu YX. Transcriptome profiling molecular biological and physiological studies reveal a major role for ethylene in cotton fiber cell elongation. , 2006, 18: 651-664[本文引用:1]
[18]
LingF, LüM, Ni ZY, Hu WR, WangJ. Digital image analysis of expansion growth of cultured cotton ovules with fibers and their responses to ABA, 2011, 47: 369-374[本文引用:1]
[19]
谭琨岭. 菜油凿醇和谷菌醇对棉花纤维发育的影响. , 2009Tan KL. The Function of Campesterol and Sitosterol for the Cotton Fiber Development. , 2009 (in Chinese with English abstract)[本文引用:1]
[20]
PangY, WangH, Song WQ, Zhu YX. The cotton ATP synthase δ1 subunit is required to maintain a higher ATP/ADP ratio that facilitates rapid fiber cell elongation. , 2010, 12: 903-909[本文引用:1]
[21]
叶春燕, 欧婷, 陈进红, 何秋伶, 祝水金. 培养基、胚龄和激素配比对棉花胚珠离体培养纤维生长发育的影响. , 2013, 25: 17-23Ye CY, OuT, Chen JH, He QL, Zhu SJ. Effect of medium, ovule age, and hormone combinations on the fiber growth and development of cotton ovule culture in vitro. , 2013, 25: 17-23 (in Chinese with English abstract)[本文引用:2]
[22]
徐楚年, 董合忠. . 北京: 中国农业大学出版社, 2006Xu CN, Dong HZ. Beijing: China Agricultural University Press, 2006 (in Chinese)[本文引用:1]
[23]
Chen JY, Wen PF, Kong WF. Effect of salicylic acid on phenylpropanoids and phenylalan in ammonialyase in harvested grapeberries. , 2006, 40: 64-72[本文引用:1]
[24]
Wolski EA, Henriouez MA, AdamlR. Induction of defense genes and secondary metabolites in saskatoons (Amelanchier alnifolia Nutt. ) in response to Entomosporium mespili using jasmonic acid and Canada milkvetch extracts. , 2010, 68: 273-282[本文引用:1]
[25]
Vom EndtD, Soarese SilvaM, Kijne JW, PasqualiG, MemelinkJ. Identification of a bipartite jasmonate-responsive promoter element in the Catharanthus roseus ORCA3 transcription factor gene that interacts specifically with AT-Hook DNA-binding proteins. , 2007, 144, 1680[本文引用:1]
[26]
马杰, 胡文忠, 毕阳, 姜爱丽, 萨仁高娃. 茉莉酸甲酯处理对鲜切莴苣和甘蓝苯丙烷代谢的影响. , 2013, 34: 333-335MaJ, Hu WZ, BiY, Jiang AL, Sarengaowa. Effect of MeJA treatments on benzene propane metabolism in tissues of fresh-cut lettuce and cabbage. , 2013, 34: 333-335 (in Chinese with English abstract)[本文引用:1]
[27]
谷晓策. 基于改造茉莉酸甲酯生物合成途径的丹参次生代谢工程新策略. , 2011Gu XC. Novel Stratage of Secondary Metabolic Engineering in Salvia Miltiorrhiza-Based on the Reform of MeJA Biosynthetic Pathway. , 2011 (in Chinese with English abstract)[本文引用:1]
[28]
Elwanm WM, Elhamahmym AM. Improved productivity and quality associated with salicylic acid application in green house pepper. , 2009, 122: 521-526[本文引用:1]
[29]
VitsevaO, VargheseS, Freedman JE. Grape seed and skin extracts inhibit platelet function and release of reactive oxygen intermediates. , 2005, 46: 445-451[本文引用:1]
[30]
黄婕. 油菜素内酯(BR)和IAA、BAP互作影响滇紫草细胞次生代谢及其生化机制研究. , 2001HuangJ. BR and IAA, BAP Interactions Influence the Secondary Metabolism of Onosma paniculatum Cell and Its Biochemical Mechanism Research. , 2001 (in Chinese with English abstract)[本文引用:1]
[31]
淦庆雷. 油菜素内酯影响滇紫草培养细胞中天然产物合成的分子机制研究. , 2004Gan QL. Molecular Mechanism for Biosynthesis of the Natural Products Affected by Brassinolide in Onosma paniculatum Cultured Cells. , 2004 (in Chinese with English abstract)[本文引用:1]
[32]
刘松江, 龚文芳, 孙君灵, 庞保印, 杜雄明. 生长物质对彩色棉胚珠离体培养纤维发育的影响. , 2015, 48: 2127-2142Liu SJ, Gong WF, Sun JL, Pang BY, DU XM. Effects of growth substances on the fiber growth and development of color cotton ovule culture in vitro. , 2015, 48: 2127-2142 (in Chinese with English abstract)[本文引用:1]
[33]
任杰, 冷平. ABA和乙烯与甜樱桃果实成熟的关系. , 2010, 37: 199-206RenJ, LengP. Role of Abscisic acid and ethylene in fruit maturation of sweet cherry. , 2010, 37: 199-206 (in Chinese with English abstract)[本文引用:1]
[34]
方荣俊, 赵华, 廖永辉. 乙烯对植物次生代谢产物合成的双重调控效应. , 2014, 49: 626-639Fang RJ, ZhaoH, Liao YH. The dual regulation effect of ethylene on plant secondary metabolites synthesis. , 2014, 49: 626-639 (in Chinese with English abstract)[本文引用:1]
[35]
刘金, 魏景立, 刘美艳. 早熟苹果花青苷积累与其相关酶活性及乙烯生成之间的关系. , 2012, 39: 1235-1242LiuJ, Wei JL, Liu MY. The relationships between the enzyme activity of anthocyanin biosynthesis, ethylene release and anthocyanin accumulation in fruits of precocious apple cultivars. , 2012, 39: 1235-1242 (in Chinese with English abstract)[本文引用:1]
[36]
SrinivasanV, CiddiV, BringiV. Metabolic inhibitors, elicitors, and precursors as tools for probing yield limitation in taxane production by Taxus chinensis cell cultures. , 1996, 12: 457-465[本文引用:1]
[37]
李贺勤, 杨华, 张娟娟, 宛晓春, 方从兵. PAL和4CL酶专一性抑制剂处理对野葛异黄酮生物合成的影响. , 2009, (1): 47-52Li HQ, YangH, Zhang JJ, Wan XC, Fang CB. The influence of PAL and 4CL enzyme specific inhibitor treatment on kudzu isoflavone biosynthesis. , 2009, (1): 47-52 (in Chinese with English abstract)[本文引用:1]
[38]
梁颖, 李加纳. 甘蓝型油菜种皮色泽形成与相关酶及蛋白质含量的影响. , 2004, 37: 522-527LiangY, Li JN. Relationship of the color formation and related enzymes and protein content of the spermoderms in Brassica napus. , 2004, 37: 522-527 (in Chinese with English abstract)[本文引用:1]