关键词:彩色棉; 纤维发育; 色素合成; 植物生长调节剂 Effects of Plant Growth Regulators on Fiber Growth and Development in Colored Cotton Ovule Culture in vitro ZHANG Xiao-Meng**, LIU Song-Jiang**, GONG Wen-Fang, SUN Jun-Ling, PANG Bao-Yin, DU Xiong-Ming* Institute of Cotton Research of Chinese Academy of Agricultural Sciences / State Key Laboratory of Cotton Biology, Anyang 455000, China Fund:This study was supported by the National Natural Science Foundation of China (31601353) AbstractNaturally colored cotton is an ideal textile raw material for the environmental protection and human health. The ovule of cotton varieties Z1-61, Lyumian CC28, and RT-baixu (CK) was cultured in the media with different concentrations of plant growth regulators (MeJA, SHAM, BR, BRz, FLD, ETH, CoCl2, PAL inhibitor, 4CL inhibitor, Urea, and Chl). After 30 days, the fiber color, fiber length, ovule fresh weight, ovule dry weight and fiber dry weight were determined and analyzed with Duncan's new multiple range method. Under the treatments of salicylhydroxamic, phenylalanine ammonia solution enzyme inhibitor and 4CL inhibitor, the color of Z1-61 and Lyumian CC28 cotton fiber was light, and the higher the concentration of the Salicylhydroxamic acid, the lighter of the cotton fiber color. BRz (2.5 μmol L-1) was conducive to the pigment accumulation in brown cotton fiber, while did not have any significant effect on the pigment appearance of green cotton. Both FLD and CoCl2 made the color of brown cotton fiber lighter, while CoCl2 had little influence on the green color cotton fiber and inhibited the formation of callus. Moreover, salicylhydroxamic acid, BRz, FLD, CoCl2, phenylalanine ammonia solution enzyme inhibitor or 4CL inhibitor decreased the fiber length, ovule fresh weight, fiber dry weight and ovule dry weight. In addition, 5 g L-1 urea or 1 mg L-1 Chl were advantageous to the brown cotton fiber pigment synthesis. In conclusion, the growth regulators tested in this study revealed the correlations among pigments synthesis, hormone, flavonoid metabolism and protein metabolism, which would be useful for developing colored cotton cultivars.
Keyword:Colored cotton; Fiber growth; Pigment synthesis; Plant growth regulators Show Figures Show Figures
4 结论茉莉酸甲酯抑制剂从反向印证了茉莉酸甲酯在棕色棉纤维伸长和色素合成过程中的关键作用; 油菜素内酯抑制剂也从反向揭示出BR在棉胚珠发育和纤维伸长中的作用; 脱落酸合成抑制剂和乙烯抑制剂处理均对胚珠和纤维发育不利, 从反向证明脱落酸和乙烯可能对纤维次生代谢物质合成有促进作用; 苯丙氨酸解氨酶(PAL)专一性抑制剂和4-香豆酸辅酶A连接酶(4CL)抑制剂的添加显著抑制棕色棉和绿色棉纤维发育和色素合成, 证实了彩色棉色素成分中黄酮类物质的存在; 适宜浓度蛋白质含量促进彩色棉纤维伸长和色素合成。 The authors have declared that no competing interests exist.
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