关键词:水稻; 淡黄叶矮化突变体; 遗传分析; 基因定位 Genetic Analysis and Gene Mapping of Yellow leaf and dwarf (yld) Mutant in Rice LI Zi-Zhuang1,2,**, XU Qian-Kun2,**, YU Hai-Ping2, ZHOU Ting-Ting2, XUE Da-Wei1, ZENG Da-Li2, GUO Long-Biao2, QIAN Qian2,*, REN De-Yong2,* 1College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310006, China
2State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
Fund:The study was supported by the National Natural Science Foundation of China (31401464). AbstractLeaf color mutants of rice are ideal materials in studies on photosynthesis, chlorophyll metabolism and chloroplast development in plants. A yellow leaf and dwarf mutant, Yellow leaf and dwarf( yld), was obtained from ethyl methane sulfonate (EMS)-treated Shuhui 527 ( Oryza sativa L.). Compared with the wild type, the yld mutant showed yellow leaf and dwarfism, and the contents of chlorophyll and carotenoid were obviously decreased. Transmission electron microscope observation revealed that the structure of most chloroplasts seemed to be normal, however, with the fuzzy grana, and fewer and looser stroma lamella in the yld mutant. Meantime, plant height, branch number, grain number per panicle, 1000-grain weight and seed-setting rate were significantly decreased, while the number of effective panicle was obviously increased in the yld mutant compared with those in the wild type. Genetic analysis showed that the yld mutant traits were controlled by a single recessive gene. The 323 mutational individuals from the F2 generation of the cross of Wuyunjing 7 and yld mutant were used for gene mapping. Finally, the YLDlocus was mapped on chromosome 11 between two Indel markers L5 and L7, with an approximate 115 kb physical region. These results would facilitate cloning and functional analysis for the YLD gene.
Keyword:Rice; Yellow leaf and dwarf mutant; Genetic analysis; Gene mapping Show Figures Show Figures
图1 野生型(左)和yld突变体(右)表型 A: 苗期; B: 分蘖期; C: 抽穗期; D: 剑叶; E: 倒二叶; F: 倒三叶。Fig. 1 Phenotypes of the wild type (left) and yld mutant (right) A: seedling stage; B: tillering stage; C: heading stage; D: flag leaf; E: second leaf from top; F: third leaf from top. Bar=10 cm.
表2 Table 2 表2(Table 2)
表2 野生型和yld突变体农艺性状 Table 2 Agronomic traits of the wild type (WT) and theyld mutant
材料 Material
株高 Plant height (cm)
有效穗数 Effective panicle
穗粒数 Grain number per panicle
结实率 Seed-setting rate (%)
千粒重 1000-grain weight (g)
一次枝梗 Primary branch number
二次枝梗 Secondary branch number
WT
104.4± 4.12
13.0± 2.51
203.0± 11.00
89.3± 5.55
33.9± 0.17
10.9± 1.00
33.9± 6.00
yld
64.6± 4.50* *
25.3± 3.51* *
52.9± 6.13* *
76.9± 3.90* *
28.6± 0.16*
7.8± 0.50* *
5.4± 0.50
* Represents significant difference between the yldmutant and wild type at the 0.05 probability level. * * Represents significant difference between the yldmutant and wild type at the 0.01 probability level. * 表示野生型与突变体在0.05水平差异显著; * * 表示野生型与突变体在0.01水平差异显著。
表2 野生型和yld突变体农艺性状 Table 2 Agronomic traits of the wild type (WT) and theyld mutant
图2 野生型和yld突变体的剑叶、倒二叶及倒三叶光合色素含量比较 A: 剑叶光合色素含量比较; B: 倒二叶光合色素含量比较; C: 倒三叶光合色素含量比较。Chl a: 叶绿素a; Chl b: 叶绿素b; Total Chl: 叶绿素a+叶绿素b; Car: 类胡萝卜素。* 表示野生型与突变体在0.05水平差异显著; * * 表示野生型与突变体在0.01水平差异显著。Fig. 2 Comparison of photosynthetic pigments of the flag, second and third leaf in the yld mutant and wild type A: comparison of photosynthetic pigments of the flag leaf; B: comparison of photosynthetic pigments of the second leaf; C: comparison of photosynthetic pigments of the third leaf. Chl a: chlorophyll a; Chl b: chlorophyll b; Total Chl: content of chlorophyll aand chlorophyll b; Car: carotenoids. * represents significant difference between theyldmutant and wild type at the 0.05 probability level; * * represents significant difference between theyldmutant and wild type at the 0.01 probability level.
图3 野生型和yld突变体的细胞超微结构 A: 野生型叶肉细胞; B: yld突变体叶肉细胞; C: 野生型叶绿体; D: yld突变体叶绿体。Fig. 3 Cell ultrastructure of the wild type and yldmutant A: mesophyll cell of the wild type; B: mesophyll cell of theyldmutant; C: chloroplast of the wild type; D: chloroplast of the yld mutant.
图4 野生型和yld突变体花粉育性 A: 野生型花粉; B: yld突变体花粉。Fig. 4 Pollen fertility in the wild type and yld mutant A: pollen fertility in the wild type; B: pollen fertility in the yld mutant. Bar = 50 μ m.
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