关键词: Pm52; 良星99; 小麦白粉病; 抗性 Pm52: Effectiveness of the Gene Conferring Resistance to Powdery Mildew in Wheat Cultivar Liangxing 99 ZOU Jing-Wei1,2, QIU Dan1,2, SUN Yan-Ling2, ZHENG Chao-Xing3, LI Jing-Ting4, WU Pei-Pei2, WU Xiao-Fei2, WANG Xiao-Ming2, ZHOU Yang2, LI Hong-Jie2,* 1 College of Life Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China
2 National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
3 College of Chemistry and Environment Engineering, Pingdingshan College, Pingdingshan 467000, China
4 College of Life Science, Beijing Normal University, Beijing 100875, China
Fund:This study was supported by the National Natural Science Foundation of China (31471491 and 31501310), the National Major Project for Developing New GM Crops (2014ZX0800906B-003), and the Agricultural Science and Technology Innovation Program of CAAS. AbstractLiangxing 99 is a powdery mildew-resistant winter wheat cultivar adapted to the Yellow-Huai Rivers Valley Facultative Wheat Zone and the Northern Winter Wheat Zone. Its powdery mildew resistance is conferred by gene Pm52 on chromosome 2BL. A total of 123 isolates of Blumeria graminisf. sp. tritici collected from different wheat producing regions were used in assessment of disease resistance, and Liangxing 99 was resistant to 80% of them. During the five consecutive growing seasons from 2012 to 2016, Liangxing 99 was immune or highly resistant to the mixture of Bgt isolate inoculated at the adult plant stage. A gene-specific marker Xgwm120 closedly linked to Pm52 and 27 Bgt isolates were used to analyze ten wheat cultivars with Liangxing 99 as a parent. Heng 4568, Hannong 2312, Zhongxinmai 99, and DH51302 may inherit Pm52 from Liangxing 99, while the powdery mildew resistance genes of Zhengmai 369 and Jimai 729 may differ from Pm52. Shi U09-4366, XR4429, Heng 10-5039, and Nongda 3486 were susceptible to all the Bgtisolates examined and did not carry gene Pm52. The responses of these cultivars at the adult plant stage were consistent with those at the seedling stage. Results from this study are favorable to facilitate the effective application of Pm52 from Liangxing 99 in breeding programs and the production of wheat.
表1 利用良星99为亲本培育的小麦品系的系谱、来源和参加国家区域试验组别 Table 1 Pedigrees, origins, and the groups tested in the national yield trials of wheat lines derived from Liangxing 99
2015年北部冬麦区北片水地组 Northern Winter Wheat Group in 2015
表1 利用良星99为亲本培育的小麦品系的系谱、来源和参加国家区域试验组别 Table 1 Pedigrees, origins, and the groups tested in the national yield trials of wheat lines derived from Liangxing 99
表2Pm52 (良星99)、Pm6 (Coker 747)和Pm33 (2636-24R)苗期对123个白粉病菌株的反应型 Table 2 Infection types of Pm52 (Liangxing 99), Pm6 (Coker 747), and Pm33 (2636-24R) produced by 123 Blumeria graminis f. sp. tritici isolates at the seedling stage
菌株 Isolate
来源 Origin
反应型 Infection type
菌株 Isolate
来源 Origin
反应型 Infection type
Pm52
Pm6
Pm33
Pm52
Pm6
Pm33
1
山东崮山 Gushan, Shandong
1
4
4
63
河北黄骅 Huanghua, Hebei
0
3
0
2
山东冠县 Guanxian, Shandong
1
0
3
64
河北涞源 Laiyuan, Hebei
0
3
0
3
山东冠县 Guanxian, Shandong
0
0
3
65
河北任丘 Renqiu, Hebei
3
3
3
4
山东冠县 Guanxian, Shandong
0
0
3
66
河北任丘 Renqiu, Hebei
4
4
3
5
山东海阳 Haiyang, Shandong
0
3
4
67
河北沙河 Shahe, Hebei
0
3
3
6
山东海阳 Haiyang, Shandong
0
3
3
68
河北沙河 Shahe, Hebei
0
2
3
7
山东济南 Jinan, Shandong
3
3
0
69
河北石家庄 Shijiazhuang, Hebei
0
2
0
8
山东济宁 Jining, Shandong
3
4
4
70
河北邢台 Xingtai, Hebei
0
3
3
9
山东济宁 Jining, Shandong
3
2
3
71
河北邢台 Xingtai, Hebei
0;
0
3
10
山东济宁 Jining, Shandong
3
3
0
72
河北邢台 Xingtai, Hebei
1
0
1
11
山东莒县 Juxian, Shandong
0
0
3
73
河北邢台 Xingtai, Hebei
0
0
0
12
山东聊城 Liaocheng, Shandong
2
3
3
74
河北徐水 Xushui, Hebei
4
3
4
13
山东聊城 Liaocheng, Shandong
1
2
3
75
河北徐水 Xushui, Hebei
1
0
4
14
山东平度 Pingdu, Shandong
4
4
4
76
河北元氏 Yuanshi, Hebei
0
4
3
15
山东平度 Pingdu, Shandong
0
2
4
77
河北正定 Zhengding, Hebei
0;
3
3
16
山东平度 Pingdu, Shandong
0
0
4
78
河北涿州 Zhuozhou, Hebei
0
1
3
17
山东平邑 Pingyi, Shandong
0
0
3
79
河北涿州 Zhuozhou, Hebei
0
3
0
18
山东平邑 Pingyi, Shandong
0
0
0
80
河北涿州 Zhuozhou, Hebei
0;
3
0
19
山东平邑 Pingyi, Shandong
0
3
0
81
河北涿州 Zhuozhou, Hebei
0
0
0
20
山东青州 Qingzhou, Shandong
0
0
0
82
河南浚县 Xunxian, Henan
0
4
3
21
山东荏平 Renping, Shandong
4
3
4
83
河南浚县 Xunxian, Henan
0
3
3
22
山东寿光 Shouguang, Shandong
0;
0
3
84
河南浚县 Xunxian, Henan
2
3
3
23
山东寿光 Shouguang, Shandong
0
0
2
85
河南开封 Kaifeng, Henan
0;
3
0
24
山东寿山 Shoushan, Shandong
3
3
3
86
河南兰考 Lankao, Henan
0
0
3
25
山东寿山 Shoushan, Shandong
0
0
3
87
河南兰考 Lankao, Henan
0
3
0
26
山东寿山 Shoushan, Shandong
0
0
0
88
河南西华 Xihua, Henan
3
3
3
27
山东文登 Wendeng, Shandong
0
3
3
89
河南西华 Xihua, Henan
3
4
3
28
山东文登 Wendeng, Shandong
3
3
0
90
河南西华 Xihua, Henan
0
4
4
29
山东武备 Wubei, Shandong
0
3
3
91
河南新乡 Xinxiang, Henan
0
3
3
30
山东武备 Wubei, Shandong
0
0
3
92
河南新乡 Xinxiang, Henan
3
2
3
31
山东烟台 Yantai, Shandong
3
4
0
93
河南新乡 Xinxiang, Henan
0
0
4
32
山东烟台 Yantai, Shandong
0
3
0
94
河南荥阳 Xingyang, Henan
1
3
0
33
山东烟台 Yantai, Shandong
1
3
2
95
河南荥阳 Xingyang, Henan
0;
3
0
34
山东烟台 Yantai, Shandong
0
0
2
96
河南郑州 Zhengzhou, Henan
0;
0
3
35
山东郓城 Yuncheng, Shandong
3
3
3
97
北京 Beijing
1
3
4
36
山东郓城 Yuncheng, Shandong
1
4
3
98
北京 Beijing
0
3
3
37
山东郓城 Yuncheng, Shandong
3
3
0
99
北京 Beijing
0;
3
3
38
山东沾化 Zhanhua, Shandong
0
0
4
100
北京 Beijing
0
3
0
39
山东沾化 Zhanhua, Shandong
0
3
3
101
北京 Beijing
0;
2
0
40
山东沾化 Zhanhua, Shandong
3
3
0
102
北京 Beijing
0
1
0
41
山东沾化 Zhanhua, Shandong
3
3
0
103
北京 Beijing
0
1
0
42
山东沾化 Zhanhua, Shandong
3
0
0
104
北京 Beijing
0
0
0
43
山东招远 Zhaoyuan, Shandong
3
3
0
105
北京 Beijing
0
1
0
44
山东招远 Zhaoyuan, Shandong
1
4
0
106
北京 Beijing
0
2
0
45
山东招远 Zhaoyuan, Shandong
0
4
0
107
北京 Beijing
0
1
1
46
山东淄博 Zibo, Shandong
0
0
3
108
山西榆次 Yuci, Shanxi
0;
3
3
47
山东淄博 Zibo, Shandong
1
0
2
109
山西榆次 Yuci, Shanxi
0
0
0
48
河北保定 Baoding, Hebei
0
2
0
110
江苏南京 Nanjing, Jiangsu
3
3
0
49
河北磁县 Cixian, Hebei
0
3
3
111
江苏南京 Nanjing, Jiangsu
0
4
0
50
河北磁县 Cixian, Hebei
0
0
3
112
江苏扬州 Yangzhou, Jiangsu
0
0
0
51
河北磁县 Cixian, Hebei
0
0
1
113
江苏扬州 Yangzhou, Jiangsu
3
3
0
52
河北定州 Dingzhou, Hebei
0;
4
3
114
江苏扬州 Yangzhou, Jiangsu
0
3
0
53
河北定州 Dingzhou, Hebei
1
0
2
115
江苏扬州 Yangzhou, Jiangsu
0
2
0
54
河北固城 Gucheng, Hebei
0
3
3
116
云南 Yunnan
0
3
3
55
河北固城 Gucheng, Hebei
1
3
3
117
贵州 Guizhou
0;
3
3
56
河北固城 Gucheng, Hebei
0
3
3
118
四川温江 Wenjiang, Sichuan
0
3
3
57
河北邯郸 Handan, Hebei
0;
3
3
119
四川温江 Wenjiang, Sichuan
1
1
3
58
河北邯郸 Handan, Hebei
0
0
4
120
四川温江 Wenjiang, Sichuan
1
1
1
59
河北邯郸 Handan, Hebei
1
0
3
121
未知 Unknown
0;
3
3
60
河北衡水 Hengshui, Hebei
0
3
3
122
未知 Unknown
1
3
1
61
河北黄骅 Huanghua, Hebei
2
4
4
123
未知 Unknown
0;
2
0
62
河北黄骅 Huanghua, Hebei
0
3
1
表2Pm52 (良星99)、Pm6 (Coker 747)和Pm33 (2636-24R)苗期对123个白粉病菌株的反应型 Table 2 Infection types of Pm52 (Liangxing 99), Pm6 (Coker 747), and Pm33 (2636-24R) produced by 123 Blumeria graminis f. sp. tritici isolates at the seedling stage
图1 良星99 (Pm52)对不同省份白粉菌菌株的苗期抗性频率括号内数字为不同省份的菌株数目。Fig. 1 Frequencies of Liangxing 99 (Pm52) against the Blumeria graminis f. sp. tritici isolates from different provinces at the seedling stageThe numbers of isolates from different provinces are shown in the brackets.
图2 良星99及10个衍生品种和感病对照品种中作9504对27个白粉菌反应型的聚类图Fig. 2 A dendrogram based on the infection types of Liangxing 99, 10 derived lines and the susceptible control cultivar Zhongzuo 9504 to 27 Blumeria graminis f. sp. tritici isolates
表4 Table 4 表4(Table 4)
表4 良星99和10个衍生品系苗期27个白粉菌菌株的反应型 Table 4 Infection type in the response to 27 Blumeria graminis f.sp.tritici isolates at seeding stage Liangxing 99 and 10 derived lines
表4 良星99和10个衍生品系苗期27个白粉菌菌株的反应型 Table 4 Infection type in the response to 27 Blumeria graminis f.sp.tritici isolates at seeding stage Liangxing 99 and 10 derived lines
表5 良星99和10个衍生品系成株期抗性反应型、倒二叶最大严重度和病程曲线下面积 Table 5 Rating score (RS) , maximum disease severity (MDS), and area under the disease progress curve (AUDPC) of Liangxing 99 and 10 derived lines at the adult stage
品种 Cultivar/line
级别 RS
倒二叶最大严重度 MDS
病程曲线下面积 AUDPC
良星99 Liangxing 99
1
0 A
0 A
衡4568 Heng 4568
3
2.3 A
18.0 A
石U09-4366 Shi U09-4366
8
84.7 B
1112.5 B
邯农2312 Hannong 2312
2
0 A
0 A
冀麦729 Jimai 729
3
2.0 A
21.0 A
郑麦369 Zhengmai 369
3
0.9A
11.0 A
XR-4429
7
92.7 C
1630.0 C
衡10-5039 Heng 10-5039
8
92.7 C
1200.0 BC
中信麦99 Zhongxinmai 99
1
0 A
0 A
DH51302
2
3.0 A
0 A
农大3486 Nongda 346
7
93.3 C
1317.5 BC
中作9504 Zhongzuo 9504
9
96.7 C
1470.0 BC
Means of MDS and AUDPC followed by different capital letters are significantly different at P < 0.01. MDS和AUDPC平均值后不同大写字母表示差异极显著(P < 0.01)
表5 良星99和10个衍生品系成株期抗性反应型、倒二叶最大严重度和病程曲线下面积 Table 5 Rating score (RS) , maximum disease severity (MDS), and area under the disease progress curve (AUDPC) of Liangxing 99 and 10 derived lines at the adult stage
图3 良星99与其衍生品系的病程曲线下面积表现图中未列出良星99、邯农2312、中信麦99和DH51302, 因其AUPDC值均为0。左侧和右侧纵坐标分别指示感病(实线)和抗病材料(虚线)的AUPDC。Fig. 3 Area under the disease progress curve (AUDPC) of Liangxing 99 and ten derived wheat linesLiangxing 99, Hannong 2312, Zhongxinmai 99, and DH51302 are not shown in the graph because their AUDPC values are 0. Left and right ordinates indicate the AUDPC of susceptible genotypes (solid line) and resistant (dot line), respectively.
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