关键词:水稻; ALK基因; 遗传多样性; 哈尼梯田; 糊化温度 Genetic Diversity of ALK Gene and Its Association with Grain Gelatinization Temperature in Currently Cultivated Rice Landraces from Hani’s Terraced Fields in Yunnan Province LI Chuang1,2,**, LIU Cheng-Chen1,2,**, ZHANG Chang-Quan1, ZHU Ji-Hui1, XU Xiao-Ying2, ZHAO Fu-Wei3, HUANG Shao-Wen4, JIN Yin-Gen2,*, LIU Qiao-Quan1,* 1 Jiangsu Key Laboratory of Crop Genetics and Physiology / Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China
2 College of Biological Science and Technology, Yangzhou University, Yangzhou 225009, China
3 Nanjing Institute of Environmental Sciences of Ministry of Environmental Protection, Nanjing 210042, China
4 Yunnan Honghe University, Mengzi 661100, China
Fund:This study was supported by the National Basic Research Program of China (2013CBA01402), the National Natural Science Foundation of China (31561143008, 31300324), and the Natural Sciences Research Project of Higher Learning Institution in Jiangsu Province (13KJB180028). AbstractYunnan province is one of the centers for genetic diversity of cultivated rice in Asia. A total of 111 rice landraces were collected from Hani’s rice terraces of Yunnan, and their population structure was analyzed by using 51 SSR markers. The 111 rice landraces were divided into japonicagroup (group I) and indicagroup (group II); however, each of the two groups was not completely clustered according to its geographical origin. Most collected landraces, accounting for 83%, belonged to indica group. Gelatinization temperature (GT) is an important parameter affecting rice cooking and eating quality. The GT of rice flour was measured, which was widely different among the collected rice varieties, showing four classes in very low ( <66°C), low (66-70°C), high (70-74°C) and very high ( >74°C) GT. Meanwhile, ALK gene, also known as SSSIIa and controlling GT, was sequenced and compared, showing ten haploid types of the ALKgene among tested samples. Association analysis, revealed that GT is mainly controlled by the combination of three SNPs of ALK gene. Among them, the A-GC or G-TT combination type controls the very low and low GT, while the combination of G-GC controls high and very high GT. SNP3402 type T is associated with high and very high GT. It was also noticed that most of the varieties from indicagroup tended to contain the G-GC mutation and thus showed a relative high GT compared with those from the japonicagroup. These results are meaningful for rice molecular breeding and germplasm resources protection.
Keyword: Oryza sativa L.; ALK gene; Genetic diversity; Hani’; s terrace; Gelatinization temperature Show Figures Show Figures
图2 供试样品稻米的糊化温度分布a~c分别为起始糊化温度(To)、最高糊化温度(Tp)和热焓值(Δ H)。Fig. 2 Distribution of gelatinization temperature among tested samplesa-c are onset temperature (To), peak temperature (Tp), and enthalpy of gelatinization (Δ H), respectively.
表2ALK基因核苷酸多态性及其与起始糊化温度(To)的关联分析 Table 2 DNA polymorphisms of ALKgene and their association with gelatinization temperature To
区域 Region
位点 Site
Hp1 (30)
Hp2 (15)
Hp3 (42)
Hp4 (15)
Hp5 (2)
Hp6 (1)
Hp7 (1)
Hp8 (2)
Hp9 (1)
Hp10 (2)
Toe
Exon1
185
G
G
G
G
G
G
G
G
A
G
0.019
264
C
C
C
C
C
C
C
G
C
C
0.005
Intron1
412
A
A
A
A
A
A
A
G
A
A
0.005
457
C
C
C
C
C
C
C
T
C
C
0.005
Exon2
516
T
T
T
T
T
T
T
C
T
T
0.005
613
G
G
G
G
G
G
G
G
G
C
0
Intron3
1381
G
G
G
G
G
G
T
G
G
G
0.012
1726
C
C
C
C
A
C
C
A
C
C
0.005
1760
G
G
G
G
C
G
G
C
G
G
0.005
1789
T
T
T
T
C
T
T
C
T
T
0.005
1798
T
T
T
T
C
T
T
C
T
T
0.005
1826
G
G
G
G
A
G
G
A
G
G
0.005
1869
—
—
—
—
T
—
—
T
—
—
0
1915
G
G
G
G
A
G
G
A
G
G
0.005
Intron6
2480
T
T
T
T
A
T
T
A
T
T
0.005
2739
C
C
C
C
A
C
C
A
C
C
0.005
Intron7
3003
G
G
G
G
T
G
G
T
G
G
0.005
2164
A
A
A
A
C
A
A
C
A
A
0.005
3168
C
C
C
C
C
G
C
C
C
C
0
3170
C
C
C
C
T
C
C
T
C
C
0.005
3176
C
C
C
C
A
C
C
A
C
C
0.005
3233
C
C
C
C
G
C
C
G
C
C
0.005
3239
C
C
C
C
A
C
C
A
C
C
0.005
3338
G
G
G
G
A
G
G
A
G
G
0.005
3402
T
C
C
C
C
C
C
C
C
C
0.093* *
3414
G
G
G
G
T
G
G
T
G
G
0.005
3437
—
—
—
—
A
—
—
A
—
—
0
Exon8
3799
A
A
A
A
G
A
A
G
A
A
0.005
3903
T
T
T
T
G
T
T
G
T
T
0.005
4198
G
A
G
G
G
G
G
G
G
G
0.211* * *
4329
G
G
G
T
G
T
G
G
G
G
0.089* *
4330
C
C
C
T
C
T
C
C
C
C
0.089* *
* * Significant at the 0.01 probability level. * * * Significant at the 0.0001 probability level.eCorrelation (R2) betweenALK nucleotide polymorphisms and To. * * 表示在0.01水平差异显著, * * * 表示在0.0001水平差异显著。e核苷酸多态性与To的关联性(R2)。
表2ALK基因核苷酸多态性及其与起始糊化温度(To)的关联分析 Table 2 DNA polymorphisms of ALKgene and their association with gelatinization temperature To
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