Flocculation effect optimization of Aspergillus niger mycelium to an energy microalgae Chlorella sp. M-12
LIU Chengyan1,2,3,, LI Chao1,2,3, LU Haifeng1,2,3,, 1.College of Water Resource and Civil Engineering, China Agriculture University, Beijing 100083, China 2.Key Laboratory of Agricultural Engineering in Structure and Environment, Ministry of Agriculture, Beijing 100083, China 3.Beijing Engineering Research Center for Livestock Poultry Healthy Environment, Beijing 100083, China
Abstract:Aiming at solving the problems of low harvesting ratio and high cost of energy microalgae collection, the mycelial of a strain of Aspergillus niger were used to flocculate Chlorella sp. M-12, which was expected to provide a rapid and effective method for nutrient recovery and microalgal cells harvesting. The optimization experiments about piggery biogas slurry treatment by using Chlorella sp. M-12, and initial inoculate size of Aspergillus niger mycelium, initial pH, temperature, and rotation speed for Chlorella sp. M-12 flocculation were implemented. In addition, the bio-flocculation mechanism was also explored. Results showed that with the initial ${\rm{NH}}_4^{+} $-N concentration of 24 mg·L?1, ${\rm{NH}}_4^{+} $-N and TP removal in piggery biogas slurry reached 84.2% and 100%, which meant consuming 1 g ${\rm{NH}}_4^{+} $-N could produce 4.3 g dry microalgal biomass. The highest Chlorella sp. M-12 harvest ratio reached above 90% under the optimal conditions with the Aspergillus niger mycelium to Chlorella sp. M-12 (dry cell weight) ratio of 1∶1, initial pH of 6.0, temperature of 28 ℃ and rotation speed of 180 r·min?1. The trapping and sweeping, and the charge neutralization effects of Aspergillus niger mycelium ball might be the main reason for the high Chlorella sp. M-12 harvest ratio. This research will provide the theoretical bases and technology support for the nutrients recovery and microalgal harvest from wastewater. Key words:energy microalgae/ resource recovery/ piggery biogas slurry/ Aspergillus niger mycelium/ bio-flocculation/ nutrients recovery.
图1不同初始氨氮浓度下猪粪沼液中${\bf{NH}}_4^{+} $-N与TP去除效果及Chlorella sp. M-12生物量随时间变化 Figure1.${\rm{NH}}_4^{+} $-N and TP removal, and Chlorella sp. M-12 biomass changes with time in different initial ${\rm{NH}}_4^{+} $-N concentration piggery biogas slurry groups
图2不同菌藻干重比、初始pH、温度、转速下Chlorella sp. M-12收获率 Figure2.Chlorella sp. M-12 harvest ratio under different Aspergillus niger mycelium dosage, initial pH, temperature and rotation speed
图5不同处理组黑曲霉菌丝球及Chlorella sp. M-12成团外观形态变化 Figure5.Appearance changes of the aggregate of Aspergillus niger mycelium and Chlorella sp. M-12 in different treatment groups
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1.College of Water Resource and Civil Engineering, China Agriculture University, Beijing 100083, China 2.Key Laboratory of Agricultural Engineering in Structure and Environment, Ministry of Agriculture, Beijing 100083, China 3.Beijing Engineering Research Center for Livestock Poultry Healthy Environment, Beijing 100083, China Received Date: 2021-06-09 Accepted Date: 2021-09-26 Available Online: 2021-11-18 Keywords:energy microalgae/ resource recovery/ piggery biogas slurry/ Aspergillus niger mycelium/ bio-flocculation/ nutrients recovery Abstract:Aiming at solving the problems of low harvesting ratio and high cost of energy microalgae collection, the mycelial of a strain of Aspergillus niger were used to flocculate Chlorella sp. M-12, which was expected to provide a rapid and effective method for nutrient recovery and microalgal cells harvesting. The optimization experiments about piggery biogas slurry treatment by using Chlorella sp. M-12, and initial inoculate size of Aspergillus niger mycelium, initial pH, temperature, and rotation speed for Chlorella sp. M-12 flocculation were implemented. In addition, the bio-flocculation mechanism was also explored. Results showed that with the initial ${\rm{NH}}_4^{+} $-N concentration of 24 mg·L?1, ${\rm{NH}}_4^{+} $-N and TP removal in piggery biogas slurry reached 84.2% and 100%, which meant consuming 1 g ${\rm{NH}}_4^{+} $-N could produce 4.3 g dry microalgal biomass. The highest Chlorella sp. M-12 harvest ratio reached above 90% under the optimal conditions with the Aspergillus niger mycelium to Chlorella sp. M-12 (dry cell weight) ratio of 1∶1, initial pH of 6.0, temperature of 28 ℃ and rotation speed of 180 r·min?1. The trapping and sweeping, and the charge neutralization effects of Aspergillus niger mycelium ball might be the main reason for the high Chlorella sp. M-12 harvest ratio. This research will provide the theoretical bases and technology support for the nutrients recovery and microalgal harvest from wastewater.
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