Effect of anode COD on electricity generation and desalination of mustard production wastewater MDC and microbial community analysis under ammonia nitrogen removal
Abstract:In this study, three-chamber microbial desalination cell (MDC) of mustard wastewater was constructed. The effects of anode chemical oxygen demand (COD) on electricity generation and desalination of mustard production wastewater MDC were investigated. And ${\rm{NH}}_4^ + $-N removal route in the desalination chamber was analyzed through the microbial community in it. The results show that the electricity generation performance of MDC at anode COD concentration of 900 mg·L?1 was better than at anode COD concentrations of 400 and 1 400 mg·L?1. Under the loading of 1 000 Ω external resistors, the output voltage, power density, and coulombic efficiency of MDC were 550 mV, 2.91 W·m?3 and (15.7±0.5)%, respectively. The desalination performance of MDC at anode COD concentration of 400 mg·L?1 was better than other two concentrations, MDC desalination time, desalination rate, and charge transfer efficiency were 910.5 h, 5.15 mg·h?1 and 111%, respectively. The ${\rm{NH}}_4^ + $-N removal route of the desalination chamber was basically same in MDC with different anode COD concentrations. Part of ${\rm{NH}}_4^ + $-N in the desalination chamber converted to ${\rm{NO}}_3^ - $-N, which was removed by its own denitrification and the migration to the anode as ${\rm{NO}}_3^ - $. Most of the remaining ${\rm{NH}}_4^ + $-N migrated to the cathode as ${\rm{NH}}_4^ + $, it converted to NH3 in an alkaline environment and emitted afterwards. The high-throughput sequencing analysis indicated that the hydrolysis fermentation bacteria (total abundance of 33.21%) were the anode core microbial community of MDC. The electrochemically active bacteria in the anode biofilm (total abundance of 11.78%) could achieve power generation of MDC. The genus of denitrifying (total abundance of 14.61%) was detected in the anode biofilm, which proved that ${\rm{NO}}_3^ - $-N in the desalination chamber could migrate to the anode chamber and the denitrification removal occurred afterwards. The genus of Ammonia (total abundance of 6.93%) and denitrifying (total abundance of 15.82%) were detected in the water of the desalination chamber, which was also the reason for ${\rm{NO}}_3^ - $-N rapid production and its subsequent steep drop in the desalination chamber. Key words:microbial desalination cell/ mustard tuber production wastewater/ anode COD/ ammonia nitrogen removal/ microbial community.
图1空气阴极MDC实验装置 Figure1.Schematic diagram of air-cathode MDC
图7单个产电周期内3室的${\rm{NH}}_4^ + $-N、${\rm{NO}}_3^ - $-N、pH及DO Figure7.${\rm{NH}}_4^ + $-N, ${\rm{NO}}_3^ - $-N, pH and DO of three chambers during single power generation cycle
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Effect of anode COD on electricity generation and desalination of mustard production wastewater MDC and microbial community analysis under ammonia nitrogen removal
1.College of Environmental and Ecology Engineering, Chongqing University, Chongqing 400045, China 2.Key Laboratory of Three Gorges Reservoir Region’s Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China Received Date: 2019-04-22 Accepted Date: 2019-05-23 Available Online: 2020-05-06 Keywords:microbial desalination cell/ mustard tuber production wastewater/ anode COD/ ammonia nitrogen removal/ microbial community Abstract:In this study, three-chamber microbial desalination cell (MDC) of mustard wastewater was constructed. The effects of anode chemical oxygen demand (COD) on electricity generation and desalination of mustard production wastewater MDC were investigated. And ${\rm{NH}}_4^ + $-N removal route in the desalination chamber was analyzed through the microbial community in it. The results show that the electricity generation performance of MDC at anode COD concentration of 900 mg·L?1 was better than at anode COD concentrations of 400 and 1 400 mg·L?1. Under the loading of 1 000 Ω external resistors, the output voltage, power density, and coulombic efficiency of MDC were 550 mV, 2.91 W·m?3 and (15.7±0.5)%, respectively. The desalination performance of MDC at anode COD concentration of 400 mg·L?1 was better than other two concentrations, MDC desalination time, desalination rate, and charge transfer efficiency were 910.5 h, 5.15 mg·h?1 and 111%, respectively. The ${\rm{NH}}_4^ + $-N removal route of the desalination chamber was basically same in MDC with different anode COD concentrations. Part of ${\rm{NH}}_4^ + $-N in the desalination chamber converted to ${\rm{NO}}_3^ - $-N, which was removed by its own denitrification and the migration to the anode as ${\rm{NO}}_3^ - $. Most of the remaining ${\rm{NH}}_4^ + $-N migrated to the cathode as ${\rm{NH}}_4^ + $, it converted to NH3 in an alkaline environment and emitted afterwards. The high-throughput sequencing analysis indicated that the hydrolysis fermentation bacteria (total abundance of 33.21%) were the anode core microbial community of MDC. The electrochemically active bacteria in the anode biofilm (total abundance of 11.78%) could achieve power generation of MDC. The genus of denitrifying (total abundance of 14.61%) was detected in the anode biofilm, which proved that ${\rm{NO}}_3^ - $-N in the desalination chamber could migrate to the anode chamber and the denitrification removal occurred afterwards. The genus of Ammonia (total abundance of 6.93%) and denitrifying (total abundance of 15.82%) were detected in the water of the desalination chamber, which was also the reason for ${\rm{NO}}_3^ - $-N rapid production and its subsequent steep drop in the desalination chamber.
实验设置3套相同规格的3室立方体空气阴极MDC反应器，均由有机玻璃板制成，如图1所示。每套反应器的阳极室、脱盐室和阴极室尺寸均相同，有效容积均为260 mL。阳极室与脱盐室中间用阴离子交换膜(AMI-7001，Membranes International Inc.，USA)分隔开，阴极室与脱盐室中间用阳离子交换膜(CMI-7000，Membranes International Inc.，USA)分隔开，构成3个腔室。阳极电极材料为碳毡(PAN基碳纤维毡，北海炭素有限公司)，有效面积为35 cm2，阴极电极材料为碳布(W1S1005，CeTech Co.，Ltd)，有效面积为42 cm2，用钛丝将电极引出，再用铜导线与变阻箱(J2361，靖江市生桐无线电配件厂)串联，形成闭合回路。3个腔室的正上方玻璃板均留有直径为6 mm的进出水孔，用以更换腔室水体。阳极室下方放置磁力搅拌器(85-2恒温磁力搅拌器，金坛市城东新瑞仪器厂)，阳极室内放置搅拌子，对阳极液进行连续搅拌。
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