兀晓鹏¹,
王庆¹,
艾庆华¹,
章北平²
1.中冶南方城市建设工程技术有限公司，武汉 430063
2.华中科技大学环境科学与工程学院，武汉 430074

Phosphorus release during denitrification process in continuous-flow intermittent- aeration biological reactor

WU Xiaopeng¹,
WANG Qing¹,
AI Qinghua¹,
ZHANG Beiping²
1.China Southern Urban Construction Engineering Technology Co.Ltd., Wuhan 430063, China
2.School of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China

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摘要:为了考察高进水ρ(C)∶ρ(N)时连续流间歇生化反应器(CIBR)中反硝化过程释磷现象，以NaAc为碳源，采用进水ρ(C)∶ρ(N)为800∶30和1 200∶30进行缺氧实验，探究反硝化过程释磷规律、机制和pH的指示作用。结果表明，2种进水ρ(C)∶ρ(N)下反硝化和释磷作用均同时发生，且先慢速释磷后快速释磷；碳源质量平衡分析得出反硝化过程中实际释磷量远小于理论释磷量，表明聚磷菌活性被抑制，部分碳源能被其他异养菌利用。反硝化过程出现释磷现象是菌群竞争碳源能力差异和反硝化中间产物抑制聚磷菌活性2个方面作用的结果；异氧菌群竞争碳源的能力顺序为反硝化菌>聚磷菌>其他异养菌，且进水碳源浓度越高，对缺氧阶段碳源利用效率越不利；pH曲线的“凸点”可指示反硝化结束，但pH无法指示释磷发生过程。该研究可为反硝化过程除磷提供初步的碳源控制依据。
关键词: 连续流间歇生化反应器/
反硝化/
释磷/
菌群竞争/
碳源控制

Abstract:Phosphorus release during denitrification process under high influent ρ(C)∶ρ(N) ratios was investigated in a bench-scale continuous-flow intermittent-aeration biological reactor (CIBR). The anoxic experiments were conducted to investigate the regularity and mechanism of phosphorus release and pH indication during denitrification process at the influent ρ(C)∶ρ(N) ratios of 800∶30 and 1 200∶30 with the carbon source of sodium acetate(NaAc). The results showed that both denitrification and phosphorus release occurred at the same time at above ρ(C)∶ρ(N) ratios, and a slow phosphorus release was followed by a fast one. The actual phosphate release from polyphosphate-accumulating organisms (PAOs) was much less than the theoretical one according to the carbon mass balance calculation, indicating that the activity of phosphorus accumulating bacteria was inhibited during denitrification process, and a part of carbon source was consumed by other heterotrophic bacteria. The phenomenon of phosphorus release during denitrification process could be ascribed to the following reasons: the differences in competitive ability on carbon source among heterotrophs and the inhibition of PAOs by denitrification intermediates. The carbon source competitiveness of heterotrophs decreased in the order denitrifiers > PAOs > other heterotrophic organisms, and the higher the influent ρ(C)∶ρ(N) ratio, the lower the carbon source utilization efficiency. During simultaneous denitrification and phosphorus release, the “bump” in the pH curve could be used as the index of the end of denitrification, but could not be used in the process of phosphorus release. This study could provide a basis for optimal carbon source control methods for phosphorus removal during denitrification process.
Key words:continous-flow intermittent-aeration biological reactor/
denitrification/
phosphorus release/
competition among heterotrophs/
carbon source control.

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刊出日期:2019-06-18

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连续流间歇生化反应器反硝化过程释磷规律

兀晓鹏¹,
王庆¹,
艾庆华¹,
章北平²
1.中冶南方城市建设工程技术有限公司，武汉 430063
2.华中科技大学环境科学与工程学院，武汉 430074
基金项目:
关键词: 连续流间歇生化反应器/
反硝化/
释磷/
菌群竞争/
碳源控制
摘要:为了考察高进水ρ(C)∶ρ(N)时连续流间歇生化反应器(CIBR)中反硝化过程释磷现象，以NaAc为碳源，采用进水ρ(C)∶ρ(N)为800∶30和1 200∶30进行缺氧实验，探究反硝化过程释磷规律、机制和pH的指示作用。结果表明，2种进水ρ(C)∶ρ(N)下反硝化和释磷作用均同时发生，且先慢速释磷后快速释磷；碳源质量平衡分析得出反硝化过程中实际释磷量远小于理论释磷量，表明聚磷菌活性被抑制，部分碳源能被其他异养菌利用。反硝化过程出现释磷现象是菌群竞争碳源能力差异和反硝化中间产物抑制聚磷菌活性2个方面作用的结果；异氧菌群竞争碳源的能力顺序为反硝化菌>聚磷菌>其他异养菌，且进水碳源浓度越高，对缺氧阶段碳源利用效率越不利；pH曲线的“凸点”可指示反硝化结束，但pH无法指示释磷发生过程。该研究可为反硝化过程除磷提供初步的碳源控制依据。

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