刘双^1,,
王莎¹,
兰兰¹,
李晓玲¹,
芦昭霖¹
1.长安大学环境科学与工程学院，西安 710064
基金项目: 国家自然科学基金资助项目51778057国家自然科学基金资助项目(51778057)

Ammonia production mechanism in a simultaneous occurrence of sulfur autotrophic and heterotrophic mixed nitrite denitrification process

LIU Shuang^1,,
WANG Sha¹,
LAN Lan¹,
LI Xiaoling¹,
LU Zhaolin¹
1.School of Environmental Science and Engineering, Chang′an University, Xi′an 710064, China

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摘要:为研究亚硝酸盐型碳、氮、硫同步脱除系统的特性，采用SBBR，以亚硝酸盐、硫化物及乙酸钠为基质，探索6种进水COD/N及5种进水S/N下碳、硫混合亚硝酸盐反硝化过程铵的生成机制。结果表明：在进水COD/N高于2、S/N高于1时，NO2--N去除率高达99%；同时，当氧化还原电位(ORP)低于-400 mV时，会出现铵浓度明显升高现象，在此条件下，进水COD/N不变时，较高的S/N会促进铵的生成；控制进水S/N不变，COD/N为3时铵浓度升高最为明显。微生物分析结果表明，该碳、氮、硫混合体系中同时存在硫自养反硝化、异养反硝化及亚硝酸盐异化还原为铵等过程，碳、硫混合亚硝酸盐反硝化过程铵的生成机制可能是低氧化还原电位和过量电子供体存在的情况下亚硝酸盐异化还原为铵的过程。
关键词: 混合反硝化/
异化亚硝酸盐还原为铵/
COD/N/
S/N

Abstract:In this study, the ammonia production mechanism by a simultaneous autotrophic and heterotrophic (mixotrophic) denitrification process was identified in a sequencing batch biofilm reactor(SBBR) fed with nitrite, sulfide and sodium acetate supplementation, and six different COD/N ratios and five different S/N ratios were designed for this purpose. The result showed that the nitrite removal efficiency was up to 99% when COD/N ratio was higher than 2 and S/N ratio was higher than 1. The increase of ammonia concentration occurred when ORP was lower than -400 mV, and high S/N ratio could enhance the ammonia production with a constant COD/N ratio in influent. While at constant S/N ratio and COD/N ratio of 3, a significant increase of ammonia yield occurred. The microbial analysis showed that sulfur autotrophic denitrification, heterotrophic denitrification as well as dissimilatory nitrite reduction to ammonia were coexisted in this carbon-nitrogen-sulfur synchronous mixing system, in which the ammonia production mechanism may be the process of dissimilatory nitrite reduction to ammonia in the presence of low redox potential and excess electron donor.
Key words:mixotrophic denitrification/
dissimilatory nitrite reduction to ammonia/
COD/N/
S/N.

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刊出日期:2019-06-18

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硫自养与异养混合亚硝酸盐反硝化过程铵生成机制

刘双^1,,
王莎¹,
兰兰¹,
李晓玲¹,
芦昭霖¹
1.长安大学环境科学与工程学院，西安 710064
基金项目: 国家自然科学基金资助项目51778057国家自然科学基金资助项目(51778057)
关键词: 混合反硝化/
异化亚硝酸盐还原为铵/
COD/N/
S/N
摘要:为研究亚硝酸盐型碳、氮、硫同步脱除系统的特性，采用SBBR，以亚硝酸盐、硫化物及乙酸钠为基质，探索6种进水COD/N及5种进水S/N下碳、硫混合亚硝酸盐反硝化过程铵的生成机制。结果表明：在进水COD/N高于2、S/N高于1时，NO2--N去除率高达99%；同时，当氧化还原电位(ORP)低于-400 mV时，会出现铵浓度明显升高现象，在此条件下，进水COD/N不变时，较高的S/N会促进铵的生成；控制进水S/N不变，COD/N为3时铵浓度升高最为明显。微生物分析结果表明，该碳、氮、硫混合体系中同时存在硫自养反硝化、异养反硝化及亚硝酸盐异化还原为铵等过程，碳、硫混合亚硝酸盐反硝化过程铵的生成机制可能是低氧化还原电位和过量电子供体存在的情况下亚硝酸盐异化还原为铵的过程。

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