李泽昌,
杨婧,
王思予,
池勾剑,
崔源源,
赵静,
刘薇
大连理工大学环境学院, 工业生态与环境工程教育部重点实验室, 大连 116024
作者简介: 宋京洋(1996-),女,硕士研究生,研究方向为环境毒理学,E-mail:songjingyang@mail.dlut.edu.cn.
基金项目: 国家重点研发计划资助项目（2016YFC0401108）；国家自然科学基金资助项目（21777020）；中央高校基本科研业务费（DUT20LK19）中图分类号: X171.5
Reduction of Cytotoxicity in Human Stem Cells by Representative Secondary Urban Sewage Treatment, Tertiary Treatment and Wetland Ecological Restoration Technology
Song Jingyang,Li Zechang,
Yang Jing,
Wang Siyu,
Chi Goujian,
Cui Yuanyuan,
Zhao Jing,
Liu Wei
Key Laboratory of Industrial Ecology and Environmental Engineering of Ministry of Eduaction, School of Environment, Dalian University of Technology, Dalian 116024, China
CLC number: X171.5
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摘要:污水中的难降解污染物往往具有浓度低但毒性强的特点，可以通过多种暴露途径直接或间接对人体健康产生有害影响，因此有必要评估污水处理技术对健康效应的削减效果。本研究选择人骨髓间充质干细胞（human bone marrow mesenchymal stem cells，hBMSCs）体外模型，评估典型二级处理、三级处理和湿地修复技术进水、主要工艺排水的综合毒性，并与发光菌急性毒性测试结果进行比较。水样萃取物对hBMSCs的细胞毒性呈剂量依赖性关系，进水毒性单位（toxicity unit，TU）在0.03~0.22范围内，出水TU为0.02~0.03。二级处理技术对细胞毒性的削减率为59.8%。三级处理过程中经混凝、微滤和反渗透处理后，出水无显著细胞毒性，但经臭氧消毒后最终排水的细胞毒性较进水无显著变化。湿地修复技术对细胞毒性的削减率为49.9%。66.7%（14/21）水样品对于hBMSCs细胞毒性的TU值高于发光菌急性毒性的TU值。在相对富集倍数（related foldenrichment，REF）20%效应浓度（EC20）下，二级处理和三级处理技术对污水hBMSCs细胞毒性的削减率低于发光菌急性毒性，但湿地修复技术对细胞毒性的削减率高于发光菌急性毒性。结果提示污水处理厂排水中残留的难降解有机污染物对人源干细胞具有较强的有害效应，为再生水水质安全性评价以及城镇污水资源化技术研发提供参考依据和方法。
关键词: 排水/
人骨髓间充质干细胞/
发光菌/
综合毒性/
毒性削减
Abstract:The refractory pollutants in the sewage wastewater usually exhibit high toxicity at low dose, which directly or indirectly pose harmful effects on human health via various exposure pathways. Therefore, it is necessary to evaluate the toxicity removal efficiency of different sewage treatment technology. In this study, human bone marrow mesenchymal stem cells (hBMSCs) was selected as an in vitro model to evaluate the integrated toxicity of the influent, the main unit process effluent from typical secondary treatment, tertiary treatment and wetland ecological restoration technology, and the final effluent, in comparison with the commonly used luminescent bacteria acute toxicity tests. The water extracts induced cytotoxicity in hBMSCs in a dose-dependent manner. The influent toxicity unit (TU) was in the range of 0.03 ~ 0.22, and the effluent TU from secondary treatment was in the range of 0.02 ~ 0.03. The toxicity was reduced by 59.8% after the secondary treatment. After coagulation, microfiltration and reverse osmosis in the tertiary treatment process, the effluent exhibited no significant cytotoxicity, but the cytotoxicity had no significant change after ozone disinfection. The cytotoxicity was reduced by 49.9% after the wetland ecological restoration treatment. The organic extracts in the sewage caused higher toxicity to hBMSCs than luminescent bacteria. Compared with the test results of the acute toxicity in luminescent bacteria, 67% (14/21) of the water samples showed higher toxicity to hBMSCs. Under 20% effect concentration for related fold enrichment (REFEC20), the reduction of cytotoxicity by secondary and tertiary treatment technology was lower than the acute toxicity of luminescent bacteria, but the reduction of wetland ecological restoration technology on the cytotoxicity of wastewater was higher. The results suggested that the residual organic pollutants in the wastewater of sewage treatment plants had detrimental effects on human stem cells. Wetland ecological restoration technology may have good prospects for reducing the cytotoxicity of sewage. The results provided referential basis and methods for the evaluation of the safety of reclaimed water quality and the development of urban sewage recycling technology.
Key words:wastewater/
human bone marrow mesenchymal stem cells (hBMSCs)/
luminescent bacteria/
integrated toxicity/
toxicity reduction.
| Niss F, Rosenmai A K, Mandava G, et al. Toxicity bioassays with concentrated cell culture media:A methodology to overcome the chemical loss by conventional preparation of water samples[J]. Environmental Science and Pollution Research, 2018, 25(12):12183-12188 |
| Xue J L, Zhang J S, Xu B, et al. Endotoxins:The critical risk factor in reclaimed water via inhalation exposure[J]. Environmental Science & Technology, 2016, 50(21):11957-11964 |
| Žegura B, Heath E, Cernoša A, et al. Combination of in vitro bioassays for the determination of cytotoxic and genotoxic potential of wastewater, surface water and drinking water samples[J]. Chemosphere, 2009, 75(11):1453-1460 |
| Shi P, Zhou S C, Xiao H X, et al. Toxicological and chemical insights into representative source and drinking water in Eastern China[J]. Environmental Pollution, 2018, 233:35-44 |
| Power E A, Boumphrey R S. International trends in bioassay use for effluent management[J]. Ecotoxicology, 2004, 13(5):377-398 |
| Li N, Wang D H, Zhou Y Q, et al. Dibutyl phthalate contributes to the thyroid receptor antagonistic activity in drinking water processes[J]. Environmental Science & Technology, 2010, 44(17):6863-6868 |
| Zhang Z, Liu W, Hu J X, et al. Surface water extracts impair gene profiles and differentiation in human mesenchymal stem cells[J]. Environment International, 2019, 132:104823 |
| Faiola F, Yin N Y, Yao X L, et al. The rise of stem cell toxicology[J]. Environmental Science & Technology, 2015, 49(10):5847-5848 |
| 赵静. 城镇污水二级处理和再生水厂三级处理综合毒性削减[D]. 大连:大连理工大学, 2019:12-14 Zhao J. Detoxification of municipal wastewater by secondary treatment and advanced treatment process in reclaimed water plant[D]. Dalian:Dalian University of Technology, 2019:12-14(in Chinese) |
| 赵静, 刘亿鑫, 邵征, 等. 城镇污水二级处理和再生水三级处理过程的雌激素活性变化[J]. 生态毒理学报, 2020, 15(2):81-86Zhao J, Liu Y X, Shao Z, et al. Variation in estrogenic activity during secondary treatment and advanced treatment process in municipal wastewater plant and reclaimed water plant[J]. Asian Journal of Ecotoxicology, 2020, 15(2):81-86(in Chinese) |
| Ma X Y, Dong K, Tang L, et al. Investigation and assessment of micropollutants and associated biological effects in wastewater treatment processes[J]. Journal of Environmental Sciences, 2020, 94:119-127 |
| Miralles-Cuevas S, Oller I, Agüera A, et al. Combination of nanofiltration and ozonation for the remediation of real municipal wastewater effluents:Acute and chronic toxicity assessment[J]. Journal of Hazardous Materials, 2017, 323:442-451 |
| Wu Q Y, Yang L L, Zhang X Y, et al. Ammonia-mediated bromate inhibition during ozonation promotes the toxicity due to organic byproduct transformation[J]. Environmental Science & Technology, 2020, 54(14):8926-8937 |
| Nasuhoglu D, Isazadeh S, Westlund P, et al. Chemical, microbial and toxicological assessment of wastewater treatment plant effluents during disinfection by ozonation[J]. Chemical Engineering Journal, 2018, 346:466-476 |
| 梁玉兰, 张小华. 不同测定方法探讨重金属对微生物毒性的影响[J]. 生态环境学报, 2019, 28(1):152-159Liang Y L, Zhang X H. Effect of heavy metals on microbial toxicity by different measuring method[J]. Ecology and Environmental Sciences, 2019, 28(1):152-159(in Chinese) |
| Ma X Y, Wang X C, Ngo H H, et al. Bioassay based luminescent bacteria:Interferences, improvements, and applications[J]. Science of the Total Environment, 2014, 468-469:1-11 |
| Roig N, Sierra J, Nadal M, et al. Relationship between pollutant content and ecotoxicity of sewage sludges from Spanish wastewater treatment plants[J]. Science of the Total Environment, 2012, 425:99-109 |
| Liang J Y, Ning X N, Sun J, et al. Toxicity evaluation of textile dyeing effluent and its possible relationship with chemical oxygen demand[J]. Ecotoxicology and Environmental Safety, 2018, 166:56-62 |
| He X X, Pelaez M, Westrick J A, et al. Efficient removal of microcystin-LR by UV-C/H2O2 in synthetic and natural water samples[J]. Water Research, 2012, 46(5):1501-1510 |
| Sun B, Wang Y, Xiang Y Y, et al. Influence of pre-ozonation of DOM on micropollutant abatement by UV-based advanced oxidation processes[J]. Journal of Hazardous Materials, 2020, 391:122201 |
