任悦3,
沈素3,
殷诺雅1,2,
Francesco Faiola1,2,
张杨3
1. 中国科学院生态环境研究中心, 环境化学与生态毒理学国家重点实验室, 北京 100085;
2. 中国科学院大学资源与环境学院, 北京 100049;
3. 首都医科大学附属北京友谊医院药学部, 北京 100050
作者简介: 杨仁君(1990-),男,博士,研究方向为干细胞毒理学,E-mail:313659164@qq.com.
基金项目: 国家自然科学基金面上项目（21577166，21876197）；国家自然科学基金青年科学基金资助项目（21707160）中图分类号: X171.5
Application and Prospect of Human Pluripotent Stem Cells in Risk Assessment of Environmental Pollutants
Yang Renjun1,2,Ren Yue3,
Shen Su3,
Yin Nuoya1,2,
Francesco Faiola1,2,
Zhang Yang3
1. State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;
2. College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China;
3. Department of Pharmacy, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
CLC number: X171.5
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摘要:在环境污染问题日益严峻的今天，人们亟需一套高效的毒理学评价体系来全面评估各类环境污染物的毒性效应和致毒机制，并阐明化合物结构与毒性效应之间的关系，进而指导安全化合物的合成。人多能干细胞（hPSCs）具有近乎无限的增殖能力和分化成所有成体细胞的潜能，近年来在毒理学的应用中崭露头角，显现出极大的应用潜力。由hPSCs分化而来的细胞可以代替原代细胞进行高通量的毒理学研究；hPSCs分化模型便于在体外研究环境污染物暴露对人体胚胎发育过程的毒性；基于hPSCs构建的类器官技术也使环境污染物的器官毒性研究成为可能。hPSCs在环境污染物风险评估中有很高的应用价值。
关键词: 环境污染物/
人多能干细胞/
发育毒性/
器官毒性
Abstract:With environmental issues being increasingly serious, an efficient toxicology evaluation system is urgently needed to comprehensively evaluate the toxic effects of various environmental pollutants, reveal the toxic mechanism, discern the relationship between the structure and toxic effects of chemicals, and thus guide the synthesis of safe compounds. In recent years, the application of human pluripotent stem cells (hPSCs) in toxicity research has emerged showing great potential. hPSCs possess almost unlimited proliferation ability and the potential to differentiate into all the cell types of the adult. Cells differentiated from hPSCs can replace primary cells for toxicity research and the experiments can be carried out in a high-throughput manner. The differentiation model of hPSCs can be used to study the developmental toxicity of environmental pollutants to human embryos in vitro. In addition, technology breakthroughs of iPSC-based organoid construction make it possible to study the organ toxicity of environmental pollutants. Therefore, hPSCs possess great practical value in risk assessment of environmental pollutants.
Key words:environmental pollutants/
human pluripotent stem cells/
developmental toxicity/
organ toxicity.
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