任晓倩1,2,
张海军1,,,
曹蓉1,
宋肖垚1,
罗云1,2,
张保琴1,
陈吉平1,,
1. 中国科学院大连化学物理研究所, 中国科学院分离分析化学重点实验室, 大连 116023;
2. 中国科学院大学, 北京 100049
作者简介: 耿柠波(1985-),女,博士,副研究员,研究方向为环境毒理学,E-mail:gengningbo@dicp.ac.cn.
通讯作者: 张海军,hjzhang@dicp.ac.cn ; 陈吉平,chenjp@dicp.ac.cn
基金项目: 国家自然科学基金委联合重大研究计划资助项目（91543201）；国家自然科学基金资助项目（21607152）中图分类号: X171.5
A Review on the Application of Proteomic Approaches in Environmental Toxicology
Geng Ningbo1,Ren Xiaoqian1,2,
Zhang Haijun1,,,
Cao Rong1,
Song Xiaoyao1,
Luo Yun1,2,
Zhang Baoqin1,
Chen Jiping1,,
1. CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China
Corresponding authors: Zhang Haijun,hjzhang@dicp.ac.cn ; Chen Jiping,chenjp@dicp.ac.cn
CLC number: X171.5
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摘要:蛋白质组学是系统生物学的重要组成部分，它以整体、动态和定量的原则来研究各种蛋白质的功能，系统地分析生物体内蛋白质表达，蛋白-蛋白相互作用和翻译后修饰等特征，已成为后基因组时代不可或缺的分析工具。蛋白质组学技术具有高通量的特征，能实现对蛋白质的高效快捷的定量分析。将蛋白质组学技术应用于环境毒理学研究，可从蛋白水平上研究外源性化合物对机体的毒性作用机制，并从中筛选出具有较高特异型和高灵敏度的蛋白标志物，为污染物的健康风险评估提供新的技术手段。本文综述了蛋白质组学技术的主要研究方法、研究策略和在环境毒理学研究中的应用，重点讨论了蛋白质组学技术在重金属和有机化合物尤其是持久性有机污染物（POPs）毒性评估中的应用。
关键词: 重金属/
有机污染物/
空气污染/
蛋白质组学/
环境毒理学
Abstract:Proteomics is an important component of system biology. It studies the functions of proteins using holistic, dynamic and quantitative approaches, and systematically analyzes the protein expressions, protein-protein interactions and post-translational modifications in living organisms. The high throughput characteristics of proteomics technology make the efficient and rapid quantitative analysis of proteins possible. Proteomics has become an indispensable analytical tool in the post-genome era. The application of proteomics in environmental toxicology study can reveal the toxic mechanism of exogenous compounds on organism and screen highly sensitive protein markers, provide a new technology for assessment of environmental pollutants. In this review, the analytical methods, research strategies and applications of proteomics in environmental toxicology were introduced. The problems and future perspective of proteomic research were also discussed. This review focused on the application of proteomic approaches in risk assessment of toxicants such as heavy metals and organic compounds, especially persistent organic pollutants (POPs).
Key words:heavy metals/
organic pollutants/
air pollution/
proteomics/
environmental toxicology.
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