郭佳葳,
周世萍^,,
刘守庆,
李惠娟,
杨发忠,
朱国磊
西南地区林业生物质资源高效利用国家林业与草原局重点实验室(西南林业大学), 昆明 650224

作者简介: 郭佳葳(1996-),女,硕士研究生,研究方向为土壤农药污染,E-mail:943495604@qq.com.

通讯作者: 周世萍,kmzhoushiping@163.com ;

基金项目: 国家自然科学基金资助项目（31860155）；云南省农业联合面上项目（2017FG001（-040））；西南地区林业生物质资源高效利用国家林业和草原局重点实验室开放基金（2019-KF13）


中图分类号: X171.5

Advances in Application of Earthworm Biomarkers in Monitoring Soil Ecosystem

Guo Jiawei,
Zhou Shiping^,,
Liu Shouqing,
Li Huijuan,
Yang Fazhong,
Zhu Guolei
Key Laboratory of State Forestry and Grassland Administration for Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China(Southwest Forestry University), Kunming 650224, China

Corresponding author: Zhou Shiping,kmzhoushiping@163.com ;

CLC number: X171.5

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摘要:随着化学物质在农业生态系统中的广泛使用，单纯的化学分析方法已经不能真实地判别出其是否会对环境产生不利影响，因此，建立有效的生物评价体系就显得尤为重要。蚯蚓广泛分布于土壤中，其生物标志物的变化对化学物质污染能起到早期监测预警作用，已经被广泛用于土壤生态系统的监测中。笔者对蚯蚓生物标志物在土壤生态系统监测中的应用进行了介绍和评述。
关键词: 土壤污染/
蚯蚓/
生态系统监测/
生物标志物

Abstract:With the widespread use of chemical substances in agricultural ecosystems, pure chemical analysis methods cannot truly distinguish whether they will have adverse effects on the environment, so it is particularly important to establish an effective biological evaluation system. Earthworms are widely distributed in the soil. The changes of their biomarkers can play an early monitoring and warning role in chemical pollution, and therefore they have been frequently used in monitoring soil ecosystems. In this paper, the application of earthworm biomarkers in soil ecosystem monitoring was introduced and reviewed.
Key words:soil pollution/
earthworms/
ecosystem monitoring/
biomarkers.

周世萍, 于泽芬, 段昌群, 等. 蚯蚓分子标记物在环境监测中的应用研究进展[J]. 环境科学导刊, 2008, 27(1):85-88Zhou S P, Yu Z F, Duan C Q, et al. Application and research of earthworm molecular marker on environmental monitoring[J]. Environmental Science Survey, 2008, 27(1):85-88(in Chinese)

肖能文, 赵彩云, 王学霞, 等. 用于土壤污染生态毒理诊断的蚯蚓生物标志物研究[J]. 江西农业学报, 2012, 24(1):137-143Xiao N W, Zhao C Y, Wang X X, et al. Studies on earthworm biomarker for eco-toxicological diagnosis of soil pollution[J]. Acta Agriculturae Jiangxi, 2012, 24(1):137-143(in Chinese)

左海根, 林玉锁, 龚瑞忠. 农药污染对蚯蚓毒性毒理研究进展[J]. 生态与农村环境学报, 2004, 20(4):68-72Zuo H G, Lin Y S, Gong R Z. Toxicology of pesticide pollution to earthworms[J]. Journal of Ecology and Rural Environment, 2004, 20(4):68-72(in Chinese)

徐冬梅, 刘文丽, 刘维屏. 外源污染物对蚯蚓毒理作用研究进展[J]. 生态毒理学报, 2009, 4(1):21-27Xu D M, Liu W L, Liu W P. Research advances in toxicological effects of external pollutants on earthworms[J]. Asian Journal of Ecotoxicology, 2009, 4(1):21-27(in Chinese)

Capowiez Y, Rault M, Costagliola G, et al. Lethal and sublethal effects of imidacloprid on two earthworm species (Aporrectodea nocturna and Allolobophora icterica)[J]. Biology and Fertility of Soils, 2005, 41(3):135-143

Espinosa-Reyes G, Ilizaliturri C A, Gonzalez-Mille D J, et al. DNA damage in earthworms (Eisenia spp.) as an indicator of environmental stress in the industrial zone of Coatzacoalcos, Veracruz, Mexico[J]. Journal of Environmental Science and Health. Part A. Toxic/Hazardous Substances & Environmental Engineering, 2010, 45(1):49-55

Mosleh Y Y, Séverine Paris-Palacios, Couderchet M, et al. Effects of the herbicide isoproturon on survival, growth rate, and protein content of mature earthworms (Lumbricus terrestris L.) and its fate in the soil[J]. Applied Soil Ecology, 2003, 23(1):69-77

段晓尘. 重金属和有机污染物对赤子爱胜蚓(Eisenia fetida)的生态毒理效应及机制差异[D]. 南京:南京农业大学, 2015:39-48 Duan X C. Ecotoxicological effects and different mechanism of heavy metal and organic pollutants on earthworm (Eisenia fetida)[D]. Nanjing:Nanjing Agricultural University, 2015:39-48(in Chinese)

崔小维. 溴氰菊酯、DEHP污染对蚯蚓种群和土壤酶的影响[D]. 沈阳:沈阳大学, 2018:36-37 Cui X W. The Effect of deltamethrin and DEHP on earthworm population and soil enzvme[D]. Shenyang:Shenyang University, 2018:36-37(in Chinese)

张露, 段碧华, 颜增光, 等. 褐潮土中外源镍对植物根伸长和蚯蚓生殖的毒性效应研究[J]. 中国农学通报, 2015, 31(33):230-235Zhang L, Duan B H, Yan Z G, et al. Toxic effect of added nickel on plant root elongation and earthworm reproduction in fluvo-aquic soil[J]. China Agricultural Bulletin, 2015, 31(33):230-235(in Chinese)

邢宇翚, 李云乐, 辛乐阳, 等. 蚯蚓对有机肥(牛粪)中铬镉铅金属富集作用的研究[J]. 北京农学院学报, 2015, 30(1):55-63Xing Y H, Li Y L, Xin L Y, et al. Bioaccumulation of chromium, cadmium, and lead in earthworms from organic fertilizer (dairy manure)[J]. Journal of Beijing Agricultural College, 2015, 30(1):55-63(in Chinese)

Calisi A, Lionetto M G, Schettino T. Biomarker response in the earthworm Lumbricus terrestris exposed to chemical pollutants[J]. Science of the Total Environment, 2011, 409(20):4456-4464

杨晓霞, 龚久平, 张伟, 等. 基于代谢组学指标的土壤亚致死剂量汞对蚯蚓的毒性研究[J]. 环境科学学报, 2019, 39(3):312-322Yang X X, Gong J P, Zhang W, et al. The toxicity of Hg in soil with sublethal doses to earthworms based on the metabolomics[J]. Acta Scientiae Circumstantiae, 2019, 39(3):312-322(in Chinese)

向昌国, 李文芳. 土壤生态环境污染的生物监测模拟研究Ⅰ.锌污染对白颈环毛蚓呼吸强度的影响[J]. 湖南农业科学, 2000(4):25-26 Xiang C G, Li W F. The influence of Zn pollution on the breathing intensity of Pheretima californica[J]. Hunan Agricultural Science, 2000(4):25-26(in Chinese)

Alshawish S A, Mohamed A I, Nair G A. Prolonged toxicity of sub-lethal dosages of chemical pesticides on the body mass and cocoons of Aporrectodea caliginosa (Oligochaeta:Lumbricidae) inhabiting Benghazi, Libya[J]. Proceedings of the Royal Society B-Biological Sciences, 2004, 74:123-133

Booth L H, O'Halloran K. A comparison of biomarker responses in the earthworm Aporrectodea caliginosa to the organophosphorus insecticides diazinon and chlorpyrifos[J]. Environmental Toxicology & Chemistry, 2010, 20(11):2494-2502

Burrows L A, Edwards C A. The use of integrated soil microcosms to assess the impact of carbendazim on soil ecosystems[J]. Ecotoxicology, 2004, 13(1-2):143-161

Casabé N, Piola L, Fuchs J, et al. Ecotoxicological assessment of the effects of glyphosate and chlorpyrifos in an Argentine soya field[J]. Journal of Soils and Sediments, 2007, 7(4):232-239

Correia F V, Moreira J C. Effects of glyphosate and 2,4-D on earthworms (Eisenia foetida) in laboratory tests[J]. Bulletin of Environmental Contamination & Toxicology, 2010, 85(3):264-268

De Silva P M C S, Pathiratne A, van Gestel C A M. Influence of temperature and soil type on the toxicity of three pesticides to Eisenia andrei[J]. Chemosphere, 2009, 76(10):1410-1415

Kreutzweiser D P, Good K P, Chartrand D T, et al. Effects on litter-dwelling earthworms and microbial decomposition of soil-applied imidacloprid for control of wood-boring insects[J]. Pest Management Science, 2008, 64(2):112-118

Mosleh Y Y, Ismail S M M, Ahmed M T, et al. Comparative toxicity and biochemical responses of certain pesticides to the mature earthworm Aporrectodea caliginosa under laboratory conditions[J]. Environmental Toxicology, 2010, 18(5):338-346

Ricketts H J, Morgan A J, Spurgeon D J, et al. Measurement of annetocin gene expression:A new reproductive biomarker in earthworm ecotoxicology[J]. Ecotoxicology and Environmental Safety, 2004, 57(1):4-10

袁方曜, 王玢, 牛振荣, 等. 华北代表性农田的蚯蚓群落与重金属污染指示研究[J]. 环境科学研究, 2004, 17(6):70-72Yuan F Y, Wang F, Niu Z R, et al. Earthworm community structure of representative agrotypes and its indicator function for heavy metals contamination in North China[J]. Research of Environmental Sciences, 2004, 17(6):70-72(in Chinese)

王振中, 张友梅, 胡觉莲, 等. 土壤重金属污染对蚯蚓(Opisthopra)影响的研究[J]. 环境科学学报, 1994, 14(2):236-243Wang Z Z, Zhang Y M, Hu J L, et al. Effect of heavy metals in soil on earthworms (Opisthopora)[J]. Acta Scientiae Circumstantiae, 1994, 14(2):236-243(in Chinese)

朱江, 杨道丽, 李志刚, 等. 作为土壤污染生物标志物的蚯蚓体腔细胞提取方法比较[J]. 上海交通大学学报:农业科学版, 2011, 29(3):1-5Zhu J, Yang D L, Li Z G, et al. Comparison studies on the methodology of extracting coelomcytes in earthworm used as a biomarker for the evaluation of the soil pollution[J]. Journal of Shanghai Jiaotong University:Agricultural Science, 2011, 29(3):1-5(in Chinese)

吴尔苗. 菲、芘对蚯蚓(Eisenia fetida)的毒性机理研究[D]. 杭州:浙江工业大学, 2011:20-28 Wu E M. Study on toxic effects of phenanthrene and pyrene to Eisenia foetida[D]. Hangzhou:Zhejiang University of Technology, 2011:20-28(in Chinese)

宋玉芳, 周启星, 许华夏, 等. 土壤重金属污染对蚯蚓的急性毒性效应研究[J]. 应用生态学报, 2002, 13(2):187-190Song Y F, Zhou Q X, Xu H X, et al. Acute toxicological effects of heavy metal pollution in soils on earthworms[J]. Journal of Applied Ecology, 2002, 13(2):187-190(in Chinese)

Zhou Q X, Zhang Q R, Liang J D. Toxic effects of acetochlor and methamidophos on earthworm Eisenia fetida in phaiozem, northeast China[J]. Journal of Environmental Sciences, 2006, 18(4):741-745

Xiao N, Jing B, Ge F, et al. The fate of herbicide acetochlor and its toxicity to Eisenia fetida under laboratory conditions[J]. Chemosphere, 2006, 62(8):1370-1373

Booth L H, Heppelthwaite V J, O'Halloran K. Growth, development and fecundity of the earthworm Aporrectodea caliginosa after exposure to two organophosphates[C] Christchurch:New Zealand Plant Protection, 2000

Yasmin S, Doris D'Souza. Effect of pesticides on the reproductive output of Eisenia fetida[J]. Bulletin of Environmental Contamination and Toxicology, 2007, 79(5):529-532

Bustos-Obregon E, Goicochea R I. Pesticide soil contamination mainly affects earthworm male reproductive parameters[J]. Asian Journal of Andrology, 2002, 4(3):195-199

解冬利. 不同类型土壤中镍的生物富集特性及其对蚯蚓的毒性效应[D]. 南京:南京农业大学, 2010:32-33 Xie D L. Nickel accumulation and toxicity to earthworm Eisenia fetida in a range of Chinese soils[D]. Nanjing:Nanjing Agricultural University, 2010:32-33(in Chinese)

高岩. 蚯蚓指示土壤重金属铜、镉污染及强化植物修复的潜力研究[D]. 北京:中国科学院研究生院, 2006:35-36 Gao Y. Study on the pollution of heavy metals copper and cadmium in soil indicated by earthworms and the potential of enhanced phytoremediation[D]. Nanjing:Graduate School of Chinese Academy of Sciences, 2006:35-36(in Chinese)

冯磊, 张兰, 张燕宁, 等. 三种新烟碱类农药对蚯蚓体重及溶酶体膜的影响[J]. 植物保护, 2015, 41(3):35-39Feng L, Zhang L, Zhang Y N, et al. Effects of three neonicotinoids on the weight and neutral red retention time of earthworm (Eisenia fetida)[J]. Plant Protection, 2015, 41(3):35-39(in Chinese)

Rajaguru P, Suba S, Palanivel M, et al. Genotoxicity of a polluted river system measured using the alkaline Comet assay on fish and earthworm tissues[J]. Environmental and Molecular Mutagenesis, 2003, 41(2):85-91

王作彬. 吡唑醚菌酯和氟嘧菌酯对蚯蚓的氧化胁迫及DNA损伤[D]. 泰安:山东农业大学, 2016:54-55 Wang Z B. Oxidative stress and DNA damage induced by pyraclostrobin and fluxystrobin exposure in earthworm[D]. Tai'an:Shandong Agricultural University, 2016:54-55(in Chinese)

Walsh P, EI Adlouni C, Nadeau D, et al. DNA adducts in earthworms exposed to a contaminated soil[J]. Soil Biology and Biochemistry, 1997, 29(3-4):721-724

卢杰, 宁寻安, 梁洁莹, 等. 印染污泥对蚯蚓的毒性效应研究[J]. 环境科学学报, 2018, 38(9):360-366Lu J, Ning X A, Liang J Y, et al.Study on the toxicity effect of textile dyeing sludge on earthworms[J]. Acta Scientiae Circumstantiae, 2018, 38(9):360-366(in Chinese)

马嵩. 钱塘江沉积物生源氮素和有毒物质多氯联苯污染特征[D]. 杭州:浙江大学, 2007:64-66 Ma S. Distribution of different forms of nitrogen and organic pollutants biological toxicity effect in sediments of Qiantang River[D]. Hangzhou:Zhejiang University, 2007:64-66(in Chinese)

王轶. 莫能菌素对蚯蚓的生态毒理效应研究[D]. 海口:海南大学, 2010:40-48 Wang Y. Ecotoxicological effects of monensin on earthworm[D]. Haikou:Hainan University, 2010:40-48(in Chinese)

张清明. 除草剂氟磺胺草醚对土壤酶、微生物与蚯蚓的生态毒理研究[D]. 泰安:山东农业大学, 2012:48-53 Zhang Q M. Ecological toxicology studies of herbicide fomesafen on soil enzyme, microorganism and earthworm (Eisenia fetida)[D]. Tai'an:Shandong Agricultural University, 2012:48-53(in Chinese)

Hooper H L, Jurkschat K, Morgan A J, et al. Comparative chronic toxicity of nanoparticulate and ionic zinc to the earthworm Eisenia veneta in a soil matrix[J]. Environment International, 2011, 37(6):1111-1117

李帅章, 孙振钧, 王冲. 铜砷单一污染对蚯蚓体腔细胞的影响[J]. 农业环境科学学报, 2008, 27(6):2382-2386Li S Z, Sun Z J, Wang C. Effects of mono-contamination of Cu and As on coelomocytes of earthworm Eisenia foetida[J]. Journal of Agro-Environment Science, 2008, 27(6):2382-2386(in Chinese)

王秋丽, 朱琳, 黄碧捷, 等. 土壤铅污染对蚯蚓体腔细胞溶酶体的毒性效应[J]. 农业环境科学学报, 2007, 26(5):1874-1878Wang Q L, Zhu L, Huang B J, et al. Toxic effects of soil lead pollution on lysosomes of earthworm coelom cells[J].Journal of Agro-Environment Science, 2007, 26(5):1874-1878(in Chinese)

周世萍, 段昌群, 刘守庆, 等. 土壤中亚致死剂量毒死蜱对蚯蚓抗氧化防御系统酶活性的影响[J]. 土壤学报, 2014, 51(2):389-393Zhou S P, Duan C Q, Liu S Q, et al. Effect of chlorpyrifos of a sublethal dose on enzyme activity of the antioxidant defense system of earthworms[J]. Acta Pedologica Sinica, 2014, 51(2):389-393(in Chinese)

张薇, 宋玉芳, 孙铁珩, 等. 菲和芘对蚯蚓(Eisenia fetida)细胞色素P450和抗氧化酶系的影响[J]. 环境化学, 2007, 26(2):202-206Zhang W, Song Y F, Sun T H, et al. Influence of phenanthrene and pyrene on cytochrome P450 and antioxidant enzymes in earthworms (Eisenia fetida)[J]. Environmental Chemistry, 2007, 26(2):202-206(in Chinese)

Rault M, Mazzia C, Capowiez Y. Tissue distribution and characterization of cholinesterase activity in six earthworm species[J]. Comparative Biochemistry & Physiology Part B Biochemistry & Molecular Biology, 2007, 147(2):340-346

Rault M, Collange B, Mazzia C, et al. Dynamics of acetylcholinesterase activity recovery in two earthworm species following exposure to ethyl-parathion[J]. Soil Biology & Biochemistry, 2008, 40(12):3086-3091

Hackenberger B K, Jarić-Perkušić D, StepićS. Effect of temephos on cholinesterase activity in the earthworm Eisenia fetida (Oligochaeta, Lumbricidae)[J]. Ecotoxicology & Environmental Safety, 2008, 71(2):583-589

马丽丽, 高雨轩, 陈微秋, 等. 三种苯基有机磷阻燃剂对蚯蚓的氧化胁迫效应[J]. 农业开发与装备, 2018(11):142-143

胡双庆. 菲和铬(Ⅵ)复合胁迫对蚯蚓抗氧化酶活性及其功能基因表达的影响[J]. 环境污染与防治, 2019, 41(1):32-36, 43 Hu S Q. Antioxidant enzyme activity and gene expression of earthworms following combined exposure to phenanthrene and chromium (Ⅵ)[J]. Environmental Pollution and Control, 2019, 41(1):32-36, 43(in Chinese)

刘修园, 赵海刚, 陈志厚, 等. 氟虫双酰胺对蚯蚓的生化毒性与细胞毒性研究[J]. 生态毒理学报, 2017, 12(4):293-301Liu X Y, Zhao H G, Chen Z H, et al. Biochemical toxicity and cytotoxicity of flubendiamide on earthworms (Eisenia fetida)[J]. Asian Journal of Ecotoxicology, 2017, 12(4):293-301(in Chinese)

李德金. 环丙沙星胁迫蚯蚓的毒性效应及蛋白质组学研究[D]. 石家庄:河北医科大学, 2015:39-46 Li D J.Toxic effect and proteomic study of earthworm Eisenia fetida exposed to ciprofloxacin[D]. Shijiazhuang:Hebei Medical University, 2015:39-46(in Chinese)

沈飞超. DMP对蚯蚓(Eisenia fetida)的毒性效应及蛋白质组学分析[D]. 杭州:浙江工业大学, 2013:38-49 Shen F C.Study on toxic effects and comparative proteomic analysis of differentially expressed proteins in the earthworm Eisenia fetida during dimethylphthalate stress[D]. Hangzhou:Zhejiang University of Technology, 2013:38-49(in Chinese)

曾燕燕, 袁涛, 申屠佳丽, 等. 蚯蚓生物标志物在土壤重金属污染评价中的应用[J]. 能源环境保护, 2013, 27(2):9-13Zeng Y Y, Yuan T, Shentu J L, et al. Assessing heavy metal contamination with earthworm biomarkers[J]. Energy Environmental Protection, 2013, 27(2):9-13(in Chinese)

丁龙, 李冬冬, 汪承润, 等. 土壤镉诱导赤子爱胜蚓的氧化损伤、防御反应及其致毒阈值[J]. 环境化学, 2014, 33(7):1115-1122Ding L, Li D D, Wang C R, et al. Oxidative damage, defense response and toxicity threshold in Eisenia foetida exposed to cadmium-polluted soils[J]. Environmental Chemistry, 2014, 33(7):1115-1122(in Chinese)

Lukkari T, Taavitsainen M, Soimasuo M, et al. Biomarker responses of the earthworm Aporrectodea tuberculata to copper and zinc exposure:Differences between populations with and without earlier metal exposure[J]. Environmental Pollution, 2004, 129(3):377-386

邹海平. 重金属对农田蚯蚓体内金属硫蛋白和相关酶活性的影响[D]. 长沙:湖南农业大学, 2015:18-24 Zou H P. Effects of heavy metal on metallothionein and enzymes activities of earthworms[D]. Changsha:Hunan Agricultural University, 2015:18-24(in Chinese)

Mustonen M, Haimi J, Vaisanen A, et al. Metallothionein gene expression differs in earthworm populations with different exposure history[J]. Ecotoxicology, 2014, 23(9):1732-1743

孔德洋, 薛银刚. 重金属镉污染对赤子爱胜蚓的自由基产生及抗氧化防御系统的影响[C]//中国毒理学会, 湖北省科学技术协会. 中国毒理学会第七次全国毒理学大会暨第八届湖北科技论坛论文集. 武汉:中国毒理学会第七次全国毒理学大会暨第八届湖北科技论坛, 2015

王晓尉. 蚯蚓分子生物标志物在土壤氯代苯胺类污染评价中的应用研究[J]. 农业环境科学学报, 2010, 29(8):1490-1494Wang X W.Applied study of earthworm molecular biomarkers in risk assessment of soil chloroaniline contamination[J]. Journal of Agro-Environment Science, 2010, 29(8):1490-1494(in Chinese)

刘嫦娥. 四种除草剂对蚯蚓生理生态的影响及其降解特征[D]. 昆明:云南大学, 2012:62-73 Liu C E.Physiological ecological effects in earthworms treated with four herbicides and their degradation in soils[D]. Kunming:Yunnan University, 2012:62-73(in Chinese)

曾燕燕. 蚯蚓对设施菜地重金属胁迫的响应及机理研究[D]. 杭州:浙江工商大学, 2014:66-76 Zeng Y Y.Toxicity response and the mechanism of the earthworm exposed to heavy metals in greenhouse soil[D]. Hangzhou:Zhejiang Gongshang University, 2014:66-76(in Chinese)

Homa J, Olchawa E, Stürzenbaum S R, et al. Early-phase immunodetection of metallothionein and heat shock proteins in extruded earthworm coelomocytes after dermal exposure to metal ions[J]. Environmental Pollution, 2005, 135(2):275-280

Saubhik H. Exposure of mussels to a polluted environment:Insights into the stress syndrome development[J]. Comparative Biochemistry & Physiology Toxicology & Pharmacology, 2010, 152(1):24-33

Ivanina A V, Taylor C, Sokolova I M. Effects of elevated temperature and cadmium exposure on stress protein response in eastern oysters Crassostrea virginica (Gmelin)[J]. Aquatic Toxicology, 2009, 91(3):245-254

Dittbrenner N, Moser I, Triebskorn R, et al. Assessment of short and long-term effects of imidacloprid on the burrowing behaviour of two earthworm species (Aporrectodea caliginosa and Lumbricus terrestris) by using 2D and 3D post-exposure techniques[J]. Chemosphere, 2011, 84(10):1349-1355

Dittbrenner N, Schmitt H, Capowiez Y, et al. Sensitivity of Eisenia fetida in comparison to Aporrectodea caliginosa and Lumbricus terrestris after imidacloprid exposure. Body mass change and histopathology[J]. Journal of Soils & Sediments, 2011, 11(6):1000-1010

Schreck E, Gontier L, Dumat C, et al. Ecological and physiological effects of soil management practices on earthworm communities in French vineyards[J]. European Journal of Soil Biology, 2012, 52:8-15