郭昌胜1,
刘淼2,
李专3,
徐建1
1. 中国环境科学研究院, 环境健康风险评估与研究中心, 北京 100012;
2. 吉林大学新能源与环境学院, 长春 130012;
3. 吉林省环境监测中心站, 长春 130011
作者简介: 陈力可(1984-),男,博士,研究方向为环境化学,E-mail:Downeychen@163.com.
基金项目: 国家自然科学基金资助项目(51208482)中图分类号: X171.5
The Cytotoxic Effects of Magnetite Nanoparticle-Pb2+ Complex on Rat Kidney Cells
Chen Like1,Guo Changsheng1,
Liu Miao2,
Li Zhuan3,
Xu Jian1
1. Center of Environmental Health Risk Assessment and Research, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;
2. College of New Energy and Environment, Jilin University, Changchun 130012, China;
3. Environmental Monitoring Center of Jilin Province, Changchun 130011, China
CLC number: X171.5
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摘要:磁铁矿纳米颗粒(magnetite nanoparticles, MNPs)是一种环境友好型吸附剂，广泛应用于废水中Pb2+的处理。目前，有不少关于MNPs毒性的研究，但对MNPs处理Pb2+形成的复合物的毒性却鲜有报道，其复合毒性亟待深入研究。本文以大鼠肾细胞(NRK)作为细胞模型，系统研究不同Pb含量的MNPs-Pb复合物，以及相应浓度的MNPs和Pb2+，对大鼠肾细胞活性、细胞形态和密度的影响，考察细胞对纳米颗粒的摄取以及细胞凋亡的作用机制，评估MNPs-Pb的毒性效应。结果表明，在本实验浓度和暴露时间(12 h)条件下，Pb2+能够显著抑制细胞活性，改变细胞形态，促进细胞凋亡，对细胞有显著的毒性效应，并且呈现剂量相关性；利用MNPs吸附水环境中Pb2+形成的复合物MNPs-Pb对大鼠肾细胞没有显著的毒性作用(P<0.05)，MNPs对Pb2+的吸附可能是Pb2+的细胞毒性降低的原因。
关键词: MNPs/
Pb2+/
复合物/
大鼠肾细胞/
细胞毒性/
细胞凋亡/
复合污染
Abstract:Magnetite nanoparticles (MNPs) are environment-friendly adsorbents, which are widely used in the treatment of Pb2+ in wastewater. Recently, significant research efforts have been made toward the investigation of MNPs toxicity, whereas very little attention has been paid to the complex of MNPs and Pb2+ (MNPs-Pb). To evaluate the potential cytotoxic effect of MNPs-Pb, rat kidney cells (NRK) were used as model cell to study the cellular internalization of MNPs-Pb and the cytotoxic effects of both MNPs-Pb and Pb2+, including cell viability, cell morphology and density, and apoptosis. It was found that under the experimental concentrations and conditions, Pb2+ could significantly inhibit cell activity, change cell morphology and promote cell apoptosis, thus displaying dose-dependent toxic effects on cells. Compared with the corresponding concentration of Pb2+, the toxicity of MNPs-Pb complex on rat kidney cells was significantly reduced. There was no significant toxic effect of MNPs-Pb on rat kidney cells.
Key words:magnetite nanoparticles/
lead/
complex/
rat kidney cells/
cytotoxicity/
apoptosis/
multiple contamination.
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