Progress on spermatogonial stem cells of large animals
Xin Zhao, Huaqiang Yang,National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China
Abstract Spermatogonial stem cells (SSCs) are male germline stem cells that reside in the basement membrane of the seminiferous tubule in the testis. SSCs are characterized by their capability of self-renewal to maintain the stem cell pool throughout the lifespan and commitments to germ line after puberty, thus transmitting the genetic information from parents to the SSC-derived progenies. SSCs can be isolated from testis, propagated in vitro, and induced to differentiate into varied germ cells. Although significant progress has been made in the field of rodent SSCs, the SSCs of large animals have advanced slowly. Studies on SSCs of large animal models can offer insights into the physiological and pathological mechanism of human reproduction. Moreover, SSCs of agricultural large animals can be used as an essential tool for multiplication of elite animal individuals, and generation of genetically modified livestock with valuable economic traits. In this review, we summarize the recent progress on SSCs of large animal models for agricultural and medical purposes, and discuss the present problems and future prospects. This review can give an overall view of large animal SSCs as respect to their applications in novel alternative reproductive technologies, generation of transgenic animals, treatment of male infertility and regenerative medicine. Keywords:spermatogonial stem cell;large animals;cell transplantation;spermatogenesis
PDF (563KB)元数据多维度评价相关文章导出EndNote|Ris|Bibtex收藏本文 本文引用格式 赵鑫, 杨化强. 大动物精原干细胞研究进展[J]. 遗传, 2019, 41(8): 686-702 doi:10.16288/j.yczz.19-167 Xin Zhao, Huaqiang Yang. Progress on spermatogonial stem cells of large animals[J]. Hereditas(Beijing), 2019, 41(8): 686-702 doi:10.16288/j.yczz.19-167
A:小鼠精原干细胞的体内发育谱系;B:小鼠体内精原干细胞不同发育阶段相对应的分子标记;C:猴精原干细胞的体内发育谱系;D:猴体内精原干细胞不同发育阶段相对应的分子标记。PGCs在胚胎的发育过程中迁移至生殖嵴后分化为生殖母细胞,生殖母细胞增殖一段时间后进入静息状态,在出生后第1周作为SSCs恢复生理活动。随着生长发育的进行,SSCs不断地增殖与分化以维持精子发生的稳态。根据参考文献[2, 4, 8, 10, 11]修改绘制。 Fig. 1Schematic of typical spermatogenesis and corresponding molecular markers in some animals
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