麻冬梅1, 4,,,
蔡进军3,
黄婷1,
马巧利2,
赵丽娟1,
张莹2
1.宁夏大学西北土地退化与生态系统恢复省部共建国家重点实验室培育基地/西北退化生态系统恢复与重建教育部重点实验室 银川 750021
2.宁夏大学农学院 银川 750021
3.宁夏农林科学院农业资源与环境研究所 银川 750021
4.宁夏优势特色作物现代分子育种重点实验室 银川 750021
基金项目: 国家自然科学基金项目31760698
宁夏农业育种专项2019NYYZ0401
宁夏重点研发项目2019BBF02022-04
宁夏高等学校一流学科建设（生态学）项目NXYLXK2017B06
详细信息
作者简介:王文静, 主要研究方向为牧草分子育种。E-mail: 1961224619@qq.com
通讯作者:麻冬梅, 主要研究方向为牧草分子育种。E-mail: 576494584@qq.com
中图分类号:Q948.1计量
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被引次数:0
出版历程
收稿日期:2020-06-25
录用日期:2020-10-16
刊出日期:2021-03-01
Photosynthetic characteristics of alfalfa seedlings under salt stress based on FvCB model
WANG Wenjing1,,MA Dongmei1, 4,,,
CAI Jinjun3,
HUANG Ting1,
MA Qiaoli2,
ZHAO Lijuan1,
ZHANG Ying2
1. Breeding Base for State Key Laboratory of Land Degradation and Ecosystem Restoration in Northwest China, Ningxia University/Key Laboratory of Restoration and Reconstruction of Degraded Ecosystems in Northwest China of Ministry of Education, Yinchuan 750021, China
2. College of Agriculture, Ningxia University, Yinchuan 750021, China
3. Institute of Agricultural Resources and Environment, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750021, China
4. Key Laboratory of Modern Molecular Breeding for Dominant Characteristic Crops in Ningxia, Yinchuan 750021, China
Funds: the National Natural Science Foundation of China31760698
the Ningxia Special Program of Agricultural Breeding2019NYYZ0401
the Ningxia Key Research and Development Program2019BBF02022-04
the First Class Discipline Construction in Ningxia Universities (Ecology)NXYLXK2017B06
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Corresponding author:MA Dongmei, E-mail: 576494584@qq.com
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摘要
摘要:探讨盐胁迫下紫花苜蓿幼苗叶片光合生理特性，可为改善紫花苜蓿生长，修复生态环境，推动牧草产业快速发展奠定基础。本研究以‘阿迪娜’为试验材料，设0 mmol·L-1（CK）、40 mmol·L-1、80 mmol·L-1、120 mmol·L-1和160 mmol·L-1共5个NaCl水平，使用Li-6400XT光合仪测定不同盐胁迫下紫花苜蓿幼苗光响应-CO2曲线，利用FvCB模型分析盐胁迫对紫花苜蓿幼苗光合特性的影响。结果表明：1）不同NaCl胁迫下叶片净光合速率（Pn）随NaCl浓度的增加而降低，与CK相比，4个NaCl胁迫下分别降低1.44%、3.85%、7.21%和7.90%，均达显著性水平（P < 0.05）；随光合有效辐射的增加均呈迅速上升趋势，CK的Pn增长速度显著高于其他处理。2）与CK相比，40 mmol·L-1和80 mmol·L-1 NaCl胁迫增加了紫花苜蓿幼苗叶片的最大羧化速率（Vcmax）和最大电子传递速率（Jmax），但120 mmol·L-1和160 mmol·L-1NaCl胁迫显著降低了Vcmax和Jmax。3）叶肉导度（gm）和暗呼吸速率（Rd）随NaCl胁迫水平的增加呈降低趋势；与CK相比，40 mmol·L-1和80 mmol·L-1 NaCl胁迫的gm变化不显著，但Rd显著降低。120 mmol·L-1和160 mmol·L-1 NaCl胁迫显著降低了gm和Rd，且与CK、40 mmol·L-1和80 mmol·L-1 NaCl胁迫间呈显著性差异。4）验证FvCB模型中子模型估算植物叶片光合的精确度，发现FvCB模型对不同胁迫处理下Pn拟合时，引入gm模型模拟精度高，平均绝对误差低。5）紫花苜蓿幼苗耐盐临界值为80~120 mmol·L-1，随NaCl浓度的增加，光合限制因素由叶肉因素转变为光合机构受损。该研究可为我国西北地区盐碱地制定有效的调控措施以提高植物耐盐能力提供科学参考。
关键词:紫花苜蓿/
盐胁迫/
FvCB模型/
最大羧化速率/
最大电子传递速率
Abstract:Understanding the photosynthetic physiological characteristics of alfalfa seedlings under salt stress is important for improving alfalfa growth, restoring the ecological environment, and promoting the development of the foraging industry in China. Alfalfa variety 'Adrenalin' seedlings were treated with different concentrations of NaCl (0 mmol·L-1, CK; 40 mmol·L-1, T1; 80 mmol·L-1, T2; 120 mmol·L-1, T3; and 160 mmol·L-1, T4), the light response curves were measured with Li-6400XT, and the effects of NaCl stress on photosynthetic characteristics were analyzed by using FvCB model. The results showed that the leaf net photosynthetic rate (Pn) decreased significantly with increasing NaCl concentration by 1.44% (T1), 7.21% (T2), 7.90% (T3), and 3.85% (T4), respectively, compared with that of CK. The Pn in all treatments showed a rapid upward trend as photosynthetic effective radiation increased, and the Pn growth rate in the normal treatment (CK) was significantly higher than that in the other treatments. Compared with those in the CK, the T1 and T2 treatments increased the alfalfa seedling leaf maximum carboxylation rate (Vcmax; by 3.59% in T1, and 13.88% in T2) and maximum electron transfer rate (Jmax; by 11.24% in T1, and 17.47% in T2), but the T3 and T4 treatments reduced Vcmax and Jmax. Leaf conductance (gm) and dark respiration rate (Rd) decreased with increasing NaCl concentration. Compared with those in the CK, the T1 and T2 treatments did not affect gm, but significantly reduced Rd. The T3 and T4 treatments significantly reduced gm and Rd compared with CK, T1, and T2 treatments. The FvCB model fitting results of alfalfa Pn under different stress treatments verified that the FvCB sub-model accurately estimated plant leaf photosynthesis, and the results showed that introducing gm into the model had a high simulation accuracy and low average absolute error. The critical salt tolerance value for alfalfa seedlings was 80–120 mmol·L-1. As the NaCl concentration increased, the photosynthetic limiting factors changed from mesophyll factors to damaged photosynthetic organs. These results may help formulate effective control measures in Northwest China saline land to improve plant salt tolerance.
Key words:Alfalfa/
Salt stress/
FvCB model/
Maximum carboxylation rate/
Maximum electron transport rate
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图1盐胁迫对紫花苜蓿幼苗叶片净光合速率的影响
不同小写字母表示不同NaCl浓度间差异显著(P < 0.05)。
Figure1.Effect of salt stress on leaves net photosynthetic rate of alfalfa seedlings
Different lowercase letters mean significant differences among NaCl concentrations at P < 0.05 level.
下载: 全尺寸图片幻灯片
图2不同盐浓度处理下紫花苜蓿幼苗叶片光响应曲线变化
Figure2.Leaves light response curves of alfalfa seedling under different salt concentrations
CK: 0 mmol·L?1 NaCl; T1: 40 mmol·L?1 NaCl; T2: 80 mmol·L?1 NaCl; T3: 120 mmol·L?1 NaCl; T4: 160 mmol·L?1 NaCl
下载: 全尺寸图片幻灯片
图3盐胁迫对紫花苜蓿叶片光合最大羧化速率(Vcmax)和最大电子传递速率(Jmax)的影响
Vcmax25和Jmax25为推算的25 ℃最大羧化速率和最大电子传递速率。
Figure3.Effects of salt stress on photosynthetic maximum carboxylation rate (Vcmax) and maximum electron transfer rate (Jmax) of alfalfa leaves
Vcmax25 and Jmax25 are calculated photosynthetic maximum carboxylation rate and maximum electron transfer rate at 25 ℃.
下载: 全尺寸图片幻灯片
图4盐胁迫对紫花苜蓿叶片叶肉导度和暗呼吸速率的影响
不同小写字母表示不同NaCl浓度间差异显著(P < 0.05)。
Figure4.Effects of salt stress on leaf conductance and dark respiration rate of alfalfa leaves
Different lowercase letters mean significant differences among NaCl concentrations at P < 0.05 level.
下载: 全尺寸图片幻灯片
图5FvCB模型预测的净光合速率和实际净光合速率的关系
P1: 引入叶肉导度(gm)推算的净光合速率; P2: 忽略gm推算的净光合速率; R2: 决定系数; MAE: 平均绝对误差。
Figure5.Relationship between predicted net photosynthetic rate by FvCB model and actual net photosynthetic rate
P1: the net photosynthetic rate involving leaf conductance (gm); P2: the net photosynthetic rate ignoring gm; R2: determination coefficient; MAE: mean absolute error.
下载: 全尺寸图片幻灯片表1FvCB模型参数及范围
Table1.Parameters and prior ranges of the FvCB model
| 参数 Parameter | 范围 Range | 单位 Unit | 描述 Description | 参考文献 Reference |
| Jmax25 | (30.3, 200) | mmol·m?2·s?1 | 25 ℃时最大电子传递速率Maximum electron transfer rate at 25 ℃ | [20-21] |
| Vmax25 | (24.3, 200) | mmol·m?2·s?1 | 25 ℃时最大羧化速率Maximum carboxylation rate at 25 ℃ | [20-21] |
| Rd25 | (0.01, 10) | mmol·m?2·s?1 | 25 ℃时暗呼吸速率Dark respiration rate at 25 ℃ | [22-23] |
| gm25 | (0.03, 10) | mmol·m?2·s?1 | 25 ℃时叶肉导度Leaf conductivity at 25 ℃ | [22-23] |
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