Auxin Regulates the Lateral Root Development Through MAPK-mediated VLCFAs Biosynthesis
Rongfeng Huang, Tongda Xu,*Horticultural Plant Biology and Metabolomics Center, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Abstract Mitogen-activated protein kinase (MAPK) cascade is an important and highly conserved cellular signal transduction pathway by delivery and amplification of upstream signals through protein kinase cascade phosphorylation in eukaryotes. In plants, MAPK signaling pathways not only mediate plant responses to environment, but also play crucial roles in regulating plant growth and development. A recent study from the Zhaojun Ding’s group of Shandong University uncovered a novel molecular mechanism of MPK14-mediated auxin signaling in lateral root development via ERF13- regulated very-long-chain fatty acids (VLCFAs) biosynthesis. This study reveals the molecular mechanism of the lateral root development from a new perspective, and further confirms the coupling between the vital phytohormone auxin and the ancient MAPKs module. Since lateral roots act as essential organs for plants in response to environment, deciphering the MAPK signaling pathway in regulation of lateral root development will provide a new strategy for how plants integrate development signals and environmental cues. Keywords：MAPK cascade;auxin;VLCFA;lateral root development
PDF (688KB)元数据多维度评价相关文章导出EndNote|Ris|Bibtex收藏本文 引用本文 黄荣峰, 徐通达. 生长素通过MAPK介导的超长链脂肪酸合成调控侧根发育. 植物学报, 2021, 56(1): 6-9 doi:10.11983/CBB20190 Huang Rongfeng, Xu Tongda. Auxin Regulates the Lateral Root Development Through MAPK-mediated VLCFAs Biosynthesis. Chinese Bulletin of Botany, 2021, 56(1): 6-9 doi:10.11983/CBB20190
生长素激活MPK信号通路后如何进一步调控侧根发育? Lv等(2020)进一步的生化和遗传实验表明, MPK14直接与乙烯响应因子ERF13互作并磷酸化ERF13, 进而调控ERF13蛋白的稳定性。有意思的是, ERF13对侧根发育的调控部分依赖于超长链脂肪酸(very-long-chain fatty acids, VLCFAs)合成途径, 通过抑制VLCFA途径中β-酮脂酰辅酶A合成酶(KCS16)的转录水平来实现。VLCFA是一类由22及更多碳原子组成的脂肪酸, 是植物种子甘油三脂、表皮和表皮蜡质以及鞘脂的组成部分。前期研究表明, VLCFA在植物的抗逆性(Seo and Park, 2011)、花粉发育(Smirnova et al., 2013)、棉纤维细胞伸长(Qin et al., 2007)、生长素极性运输(Roudier et al., 2010)以及有丝分裂赤道板形成(Bach et al., 2011)等过程中发挥重要作用。
VLCFA是细胞壁的重要组分(Bach and Faure, 2010)。Lv等(2020)研究表明, VLCFA通过影响细胞壁成分果胶质的降解来限制侧根突破内皮层, 从而导致成形侧根数量减少, 而过量表达KCS16或外源施加VLCFA均可恢复ERF13过量表达植株中侧根突破障碍的缺陷表型, 最终证明VLCFA在植物侧根发育过程中具有重要作用。有意思的是, 胡玉欣课题组发现VLCFA及其衍生物作为细胞层特异的信号分子, 抑制植物中柱鞘类细胞命运转变, 从而调控愈伤组织的形成(Shang et al., 2016)。在拟南芥中, 侧根的起始和愈伤组织的形成均源于中柱鞘细胞的转分化(Che et al., 2007; Atta et al., 2009; Sugimoto et al., 2010), 而VLCFA在这2个过程中都发挥至关重要的作用, 表明VLCFA可能参与植物细胞分化的重要过程。
AttaR, LaurensL, Boucheron-DubuissonE, Guivarc'hA, CarneroE, Giraudat-PautotV, RechP, ChriquiD (2009). Pluripotency of Arabidopsis xylem pericycle underlies shoot regeneration from root and hypocotyl explants grown in vitro Plant J 57, 626-644. URLPMID:18980654 [本文引用: 1]
BachL, FaureJD (2010). Role of very-long-chain fatty acids in plant development, when chain length does matter Comptes Rendus Biologies 333, 361-370. URLPMID:20371111 [本文引用: 1]
BachL, GissotL, MarionJ, TellierF, MoreauP, Satiat-Jeunema?treB, PalauquiJC, NapierJA, FaureJD (2011). Very-long-chain fatty acids are required for cell plate formation during cytokinesis in Arabidopsis thaliana J Cell Sci 124, 3223-3234. [本文引用: 1]
CheP, LallS, HowellSH (2007). Developmental steps in acquiring competence for shoot development in Arabidopsis tissue culture Planta 226, 1183-1194. URLPMID:17581762 [本文引用: 1]
De RybelB, VassilevaV, ParizotB, DemeulenaereM, GrunewaldW, AudenaertD, Van CampenhoutJ, OvervoordeP, JansenL, VannesteS, M?llerB, WilsonM, HolmanT, Van IsterdaelG, BrunoudG, VuylstekeM, VernouxT, De VeylderL, InzéD, WeijersD, BennettMJ, BeeckmanT (2010). A novel aux/IAA28 signaling cascade activates GATA23-dependent specification of lateral root founder cell identity Curr Biol 20, 1697-1706. [本文引用: 1]
GohT, KasaharaH, MimuraT, KamiyaY, FukakiH (2012). Multiple AUX/IAA-ARF modules regulate lateral root formation: the role of Arabidopsis SHY2/IAA3 -mediated auxin signaling Philos Trans Roy Soc B: Biol Sci 367, 1461-1468. [本文引用: 1]
HamannT, BenkovaE, BaurleI, KientzM, JürgensG (2002). The Arabidopsis BODENLOS gene encodes an auxin response protein inhibiting MONOPTEROS-mediated embryo patterning Genes Dev 16, 1610-1615. [本文引用: 1]
RoudierF, GissotL, BeaudoinF, HaslamR, MichaelsonL, MarionJ, MolinoD, LimaA, BachL, MorinH, TellierF, PalauquiJC, BellecY, RenneC, MiquelM, DaCostaM, VignardJ, RochatC, MarkhamJE, MoreauP, NapierJ, FaureJD (2010). Very-long-chain fatty acids are involved in polar auxin transport and developmental patterning in Arabidopsis Plant Cell 22, 364-375. [本文引用: 1]
SeoPJ, ParkCM (2011). Cuticular wax biosynthesis as a way of inducing drought resistance Plant Signal Behav 6, 1043-1045. [本文引用: 1]
ShangBS, XuCY, ZhangXX, CaoHF, XinW, HuYX (2016). Very-long-chain fatty acids restrict regeneration capacity by confining pericycle competence for callus formation in Arabidopsis Proc Natl Acad Sci USA 113, 5101-5106. DOI:10.1073/pnas.1522466113URLPMID:27092001 [本文引用: 1] The already differentiated organs in plants have a remarkable capacity to regenerate new individuals under culture conditions. Plant in vitro regeneration practically starts with the induction of a pluripotent cell mass, the callus, from detached organs on auxin-rich callus-inducing medium (CIM), which is generally required for subsequent regeneration of new bodies. Recent studies show that CIM-induced callus formation occurs from the pericycle or pericycle-like cells through a root developmental pathway, whereas the signals involved in governing callus-forming capacity of pericycle cells remain unknown. Here we report that very-long-chain fatty acids (VLCFAs) play a critical role in confining the pericycle competence for callus formation and thus the regeneration capacity of Arabidopsis By genetic screening, we identified the callus formation-related 1 (cfr1) mutant, which bypasses the inhibition of callus-forming capacity in roots by solitary-root (slr/iaa14). We show that CFR1 encodes 3-ketoacyl-CoA synthase 1 (KCS1), which catalyzes a rate-limiting step of VLCFA biosynthesis. Our biochemical and genetic analyses demonstrate that VLCFAs restrict the pericycle competence for callus formation, at least in part, by regulating the transcription of Aberrant Lateral Root Formation 4 (ALF4). Moreover, we provide evidence that VLCFAs act as cell layer signals to mediate the pericycle competence for callus formation. Taken together, our results identify VLCFAs or their derivatives as the confining signals for mediating the pericycle competence for callus formation and thus the regeneration capacity of plant organs.
SmirnovaA, LeideJ, RiedererM (2013). Deficiency in a very-long-chain fatty acid β-ketoacyl-coenzyme a synthase of tomato impairs microgametogenesis and causes floral organ fusion Plant Physiol 161, 196-209. DOI:10.1104/pp.112.206656URL [本文引用: 1] Previously, it was shown that beta-ketoacyl-coenzyme A synthase ECERIFERUM6 (CER6) is necessary for the biosynthesis of very-long-chain fatty acids with chain lengths beyond C-28 in tomato (Solanum lycopersicum) fruits and C-26 in Arabidopsis (Arabidopsis thaliana) leaves and the pollen coat. CER6 loss of function in Arabidopsis resulted in conditional male sterility, since pollen coat lipids are responsible for contact-mediated pollen hydration. In tomato, on the contrary, pollen hydration does not rely on pollen coat lipids. Nevertheless, mutation in SlCER6 impairs fertility and floral morphology. Here, the contribution of SlCER6 to the sexual reproduction and flower development of tomato was addressed. Cytological analysis and cross-pollination experiments revealed that the slcer6 mutant has male sterility caused by (1) hampered pollen dispersal and (2) abnormal tapetum development. SlCER6 loss of function provokes a decrease of n- and iso-alkanes with chain lengths of C-27 or greater and of anteiso-alkanes with chain lengths of C-28 or greater in flower cuticular waxes, but it has no impact on flower cuticle ultrastructure and cutin content. Expression analysis confirmed high transcription levels of SlCER6 in the anther and the petal, preferentially in sites subject to epidermal fusion. Hence, wax deficiency was proposed to be the primary reason for the flower fusion phenomenon in tomato. The SlCER6 substrate specificity was revisited. It might be involved in elongation of not only linear but also branched very-long-chain fatty acids, leading to production of the corresponding alkanes. SlCER6 implements a function in the sexual reproduction of tomato that is different from the one in Arabidopsis: SlCER6 is essential for the regulation of timely tapetum degradation and, consequently, microgametogenesis.
SugimotoK, JiaoYL, MeyerowitzEM (2010). Arabidopsis regeneration from multiple tissues occurs via a root development pathway Dev Cell 18, 463-471. DOI:10.1016/j.devcel.2010.02.004URL [本文引用: 1]
XuJ, ZhangSQ (2015). Mitogen-activated protein kinase cascades in signaling plant growth and development Trends Plant Sci 20, 56-64. [本文引用: 1]
ZhuQK, ShaoYM, GeST, ZhangMM, ZhangTS, HuXT, LiuYD, WalkerJ, ZhangSQ, XuJ (2019). A MAPK cascade downstream of IDA-HAE/HSL2 ligand-receptor pair in lateral root emergence Nat Plants 5, 414-423. [本文引用: 2]
新中国成立70年来植物激素研究进展 1 2019
... 根系是植物固着生长的重要器官, 具有很强的可塑性, 以适应周边复杂且变化的环境.根系主要由主根和侧根组成.与主根不同, 侧根的形成属于胚后发育过程, 由中柱鞘细胞经命运转变而来.侧根的发育过程包括建成细胞的命运确定、侧根原基的形态建成以及侧根的成形(Malamy and Benfey, 1997; Péret et al., 2009; He and Meng, 2020).前期研究发现, 侧根发育的一系列过程都与生长素的时空分布有关, 其中侧根起始位置与生长素沿主根的节律性振荡有关, 而侧根发育又与局部生长素的有序累积有关(黎家和李传友, 2019).生长素通过TIR1/AFB-Aux/IAA介导的转录调控模式精准调控侧根发育的各个过程, 包括通过TRI1-IAA28-ARF5/6/7/8/19调控GATA23 (GA- TA-type transcription factor 23)基因的表达, 诱发侧根建成细胞的命运转变(De Rybel et al., 2010); 通过激活IAA14-ARF7/19和IAA12-ARF5协同影响中柱鞘细胞核的位移, 驱动早期侧根原基的不对称分裂(Hamann et al., 2002; Lee et al., 2009); 通过IAA3-ARF7和IAA14-ARF7/19促进CWR (cell wall remodeling)基因的表达, 消融细胞壁, 从而使侧根原基突破内皮层并外露成形(Goh et al., 2012; Lavenus et al., 2013; Zhu et al., 2019). ...
Pluripotency of Arabidopsis xylem pericycle underlies shoot regeneration from root and hypocotyl explants grown in vitro 1 2009
... VLCFA是细胞壁的重要组分(Bach and Faure, 2010).Lv等(2020)研究表明, VLCFA通过影响细胞壁成分果胶质的降解来限制侧根突破内皮层, 从而导致成形侧根数量减少, 而过量表达KCS16或外源施加VLCFA均可恢复ERF13过量表达植株中侧根突破障碍的缺陷表型, 最终证明VLCFA在植物侧根发育过程中具有重要作用.有意思的是, 胡玉欣课题组发现VLCFA及其衍生物作为细胞层特异的信号分子, 抑制植物中柱鞘类细胞命运转变, 从而调控愈伤组织的形成(Shang et al., 2016).在拟南芥中, 侧根的起始和愈伤组织的形成均源于中柱鞘细胞的转分化(Che et al., 2007; Atta et al., 2009; Sugimoto et al., 2010), 而VLCFA在这2个过程中都发挥至关重要的作用, 表明VLCFA可能参与植物细胞分化的重要过程. ...
Role of very-long-chain fatty acids in plant development, when chain length does matter 1 2010
... VLCFA是细胞壁的重要组分(Bach and Faure, 2010).Lv等(2020)研究表明, VLCFA通过影响细胞壁成分果胶质的降解来限制侧根突破内皮层, 从而导致成形侧根数量减少, 而过量表达KCS16或外源施加VLCFA均可恢复ERF13过量表达植株中侧根突破障碍的缺陷表型, 最终证明VLCFA在植物侧根发育过程中具有重要作用.有意思的是, 胡玉欣课题组发现VLCFA及其衍生物作为细胞层特异的信号分子, 抑制植物中柱鞘类细胞命运转变, 从而调控愈伤组织的形成(Shang et al., 2016).在拟南芥中, 侧根的起始和愈伤组织的形成均源于中柱鞘细胞的转分化(Che et al., 2007; Atta et al., 2009; Sugimoto et al., 2010), 而VLCFA在这2个过程中都发挥至关重要的作用, 表明VLCFA可能参与植物细胞分化的重要过程. ...
Very-long-chain fatty acids are required for cell plate formation during cytokinesis in Arabidopsis thaliana 1 2011
... 生长素激活MPK信号通路后如何进一步调控侧根发育? Lv等(2020)进一步的生化和遗传实验表明, MPK14直接与乙烯响应因子ERF13互作并磷酸化ERF13, 进而调控ERF13蛋白的稳定性.有意思的是, ERF13对侧根发育的调控部分依赖于超长链脂肪酸(very-long-chain fatty acids, VLCFAs)合成途径, 通过抑制VLCFA途径中β-酮脂酰辅酶A合成酶(KCS16)的转录水平来实现.VLCFA是一类由22及更多碳原子组成的脂肪酸, 是植物种子甘油三脂、表皮和表皮蜡质以及鞘脂的组成部分.前期研究表明, VLCFA在植物的抗逆性(Seo and Park, 2011)、花粉发育(Smirnova et al., 2013)、棉纤维细胞伸长(Qin et al., 2007)、生长素极性运输(Roudier et al., 2010)以及有丝分裂赤道板形成(Bach et al., 2011)等过程中发挥重要作用. ...
Developmental steps in acquiring competence for shoot development in Arabidopsis tissue culture 1 2007
... VLCFA是细胞壁的重要组分(Bach and Faure, 2010).Lv等(2020)研究表明, VLCFA通过影响细胞壁成分果胶质的降解来限制侧根突破内皮层, 从而导致成形侧根数量减少, 而过量表达KCS16或外源施加VLCFA均可恢复ERF13过量表达植株中侧根突破障碍的缺陷表型, 最终证明VLCFA在植物侧根发育过程中具有重要作用.有意思的是, 胡玉欣课题组发现VLCFA及其衍生物作为细胞层特异的信号分子, 抑制植物中柱鞘类细胞命运转变, 从而调控愈伤组织的形成(Shang et al., 2016).在拟南芥中, 侧根的起始和愈伤组织的形成均源于中柱鞘细胞的转分化(Che et al., 2007; Atta et al., 2009; Sugimoto et al., 2010), 而VLCFA在这2个过程中都发挥至关重要的作用, 表明VLCFA可能参与植物细胞分化的重要过程. ...
A novel aux/IAA28 signaling cascade activates GATA23-dependent specification of lateral root founder cell identity 1 2010
... 根系是植物固着生长的重要器官, 具有很强的可塑性, 以适应周边复杂且变化的环境.根系主要由主根和侧根组成.与主根不同, 侧根的形成属于胚后发育过程, 由中柱鞘细胞经命运转变而来.侧根的发育过程包括建成细胞的命运确定、侧根原基的形态建成以及侧根的成形(Malamy and Benfey, 1997; Péret et al., 2009; He and Meng, 2020).前期研究发现, 侧根发育的一系列过程都与生长素的时空分布有关, 其中侧根起始位置与生长素沿主根的节律性振荡有关, 而侧根发育又与局部生长素的有序累积有关(黎家和李传友, 2019).生长素通过TIR1/AFB-Aux/IAA介导的转录调控模式精准调控侧根发育的各个过程, 包括通过TRI1-IAA28-ARF5/6/7/8/19调控GATA23 (GA- TA-type transcription factor 23)基因的表达, 诱发侧根建成细胞的命运转变(De Rybel et al., 2010); 通过激活IAA14-ARF7/19和IAA12-ARF5协同影响中柱鞘细胞核的位移, 驱动早期侧根原基的不对称分裂(Hamann et al., 2002; Lee et al., 2009); 通过IAA3-ARF7和IAA14-ARF7/19促进CWR (cell wall remodeling)基因的表达, 消融细胞壁, 从而使侧根原基突破内皮层并外露成形(Goh et al., 2012; Lavenus et al., 2013; Zhu et al., 2019). ...
Multiple AUX/IAA-ARF modules regulate lateral root formation: the role of Arabidopsis SHY2/IAA3 -mediated auxin signaling 1 2012
... 根系是植物固着生长的重要器官, 具有很强的可塑性, 以适应周边复杂且变化的环境.根系主要由主根和侧根组成.与主根不同, 侧根的形成属于胚后发育过程, 由中柱鞘细胞经命运转变而来.侧根的发育过程包括建成细胞的命运确定、侧根原基的形态建成以及侧根的成形(Malamy and Benfey, 1997; Péret et al., 2009; He and Meng, 2020).前期研究发现, 侧根发育的一系列过程都与生长素的时空分布有关, 其中侧根起始位置与生长素沿主根的节律性振荡有关, 而侧根发育又与局部生长素的有序累积有关(黎家和李传友, 2019).生长素通过TIR1/AFB-Aux/IAA介导的转录调控模式精准调控侧根发育的各个过程, 包括通过TRI1-IAA28-ARF5/6/7/8/19调控GATA23 (GA- TA-type transcription factor 23)基因的表达, 诱发侧根建成细胞的命运转变(De Rybel et al., 2010); 通过激活IAA14-ARF7/19和IAA12-ARF5协同影响中柱鞘细胞核的位移, 驱动早期侧根原基的不对称分裂(Hamann et al., 2002; Lee et al., 2009); 通过IAA3-ARF7和IAA14-ARF7/19促进CWR (cell wall remodeling)基因的表达, 消融细胞壁, 从而使侧根原基突破内皮层并外露成形(Goh et al., 2012; Lavenus et al., 2013; Zhu et al., 2019). ...
The Arabidopsis BODENLOS gene encodes an auxin response protein inhibiting MONOPTEROS-mediated embryo patterning 1 2002
... 根系是植物固着生长的重要器官, 具有很强的可塑性, 以适应周边复杂且变化的环境.根系主要由主根和侧根组成.与主根不同, 侧根的形成属于胚后发育过程, 由中柱鞘细胞经命运转变而来.侧根的发育过程包括建成细胞的命运确定、侧根原基的形态建成以及侧根的成形(Malamy and Benfey, 1997; Péret et al., 2009; He and Meng, 2020).前期研究发现, 侧根发育的一系列过程都与生长素的时空分布有关, 其中侧根起始位置与生长素沿主根的节律性振荡有关, 而侧根发育又与局部生长素的有序累积有关(黎家和李传友, 2019).生长素通过TIR1/AFB-Aux/IAA介导的转录调控模式精准调控侧根发育的各个过程, 包括通过TRI1-IAA28-ARF5/6/7/8/19调控GATA23 (GA- TA-type transcription factor 23)基因的表达, 诱发侧根建成细胞的命运转变(De Rybel et al., 2010); 通过激活IAA14-ARF7/19和IAA12-ARF5协同影响中柱鞘细胞核的位移, 驱动早期侧根原基的不对称分裂(Hamann et al., 2002; Lee et al., 2009); 通过IAA3-ARF7和IAA14-ARF7/19促进CWR (cell wall remodeling)基因的表达, 消融细胞壁, 从而使侧根原基突破内皮层并外露成形(Goh et al., 2012; Lavenus et al., 2013; Zhu et al., 2019). ...
MAPK signaling: emerging roles in lateral root formation 1 2020
... 根系是植物固着生长的重要器官, 具有很强的可塑性, 以适应周边复杂且变化的环境.根系主要由主根和侧根组成.与主根不同, 侧根的形成属于胚后发育过程, 由中柱鞘细胞经命运转变而来.侧根的发育过程包括建成细胞的命运确定、侧根原基的形态建成以及侧根的成形(Malamy and Benfey, 1997; Péret et al., 2009; He and Meng, 2020).前期研究发现, 侧根发育的一系列过程都与生长素的时空分布有关, 其中侧根起始位置与生长素沿主根的节律性振荡有关, 而侧根发育又与局部生长素的有序累积有关(黎家和李传友, 2019).生长素通过TIR1/AFB-Aux/IAA介导的转录调控模式精准调控侧根发育的各个过程, 包括通过TRI1-IAA28-ARF5/6/7/8/19调控GATA23 (GA- TA-type transcription factor 23)基因的表达, 诱发侧根建成细胞的命运转变(De Rybel et al., 2010); 通过激活IAA14-ARF7/19和IAA12-ARF5协同影响中柱鞘细胞核的位移, 驱动早期侧根原基的不对称分裂(Hamann et al., 2002; Lee et al., 2009); 通过IAA3-ARF7和IAA14-ARF7/19促进CWR (cell wall remodeling)基因的表达, 消融细胞壁, 从而使侧根原基突破内皮层并外露成形(Goh et al., 2012; Lavenus et al., 2013; Zhu et al., 2019). ...
Noncanonical auxin signaling regulates cell division pattern during lateral root development 1 2019
Lateral root development in Arabidopsis: fifty shades of auxin 1 2013
... 根系是植物固着生长的重要器官, 具有很强的可塑性, 以适应周边复杂且变化的环境.根系主要由主根和侧根组成.与主根不同, 侧根的形成属于胚后发育过程, 由中柱鞘细胞经命运转变而来.侧根的发育过程包括建成细胞的命运确定、侧根原基的形态建成以及侧根的成形(Malamy and Benfey, 1997; Péret et al., 2009; He and Meng, 2020).前期研究发现, 侧根发育的一系列过程都与生长素的时空分布有关, 其中侧根起始位置与生长素沿主根的节律性振荡有关, 而侧根发育又与局部生长素的有序累积有关(黎家和李传友, 2019).生长素通过TIR1/AFB-Aux/IAA介导的转录调控模式精准调控侧根发育的各个过程, 包括通过TRI1-IAA28-ARF5/6/7/8/19调控GATA23 (GA- TA-type transcription factor 23)基因的表达, 诱发侧根建成细胞的命运转变(De Rybel et al., 2010); 通过激活IAA14-ARF7/19和IAA12-ARF5协同影响中柱鞘细胞核的位移, 驱动早期侧根原基的不对称分裂(Hamann et al., 2002; Lee et al., 2009); 通过IAA3-ARF7和IAA14-ARF7/19促进CWR (cell wall remodeling)基因的表达, 消融细胞壁, 从而使侧根原基突破内皮层并外露成形(Goh et al., 2012; Lavenus et al., 2013; Zhu et al., 2019). ...
LBD18/ ASL20 regulates lateral root formation in combination with LBD16/ASL18 downstream of ARF7 and ARF19 in Arabidopsis 1 2009
... 根系是植物固着生长的重要器官, 具有很强的可塑性, 以适应周边复杂且变化的环境.根系主要由主根和侧根组成.与主根不同, 侧根的形成属于胚后发育过程, 由中柱鞘细胞经命运转变而来.侧根的发育过程包括建成细胞的命运确定、侧根原基的形态建成以及侧根的成形(Malamy and Benfey, 1997; Péret et al., 2009; He and Meng, 2020).前期研究发现, 侧根发育的一系列过程都与生长素的时空分布有关, 其中侧根起始位置与生长素沿主根的节律性振荡有关, 而侧根发育又与局部生长素的有序累积有关(黎家和李传友, 2019).生长素通过TIR1/AFB-Aux/IAA介导的转录调控模式精准调控侧根发育的各个过程, 包括通过TRI1-IAA28-ARF5/6/7/8/19调控GATA23 (GA- TA-type transcription factor 23)基因的表达, 诱发侧根建成细胞的命运转变(De Rybel et al., 2010); 通过激活IAA14-ARF7/19和IAA12-ARF5协同影响中柱鞘细胞核的位移, 驱动早期侧根原基的不对称分裂(Hamann et al., 2002; Lee et al., 2009); 通过IAA3-ARF7和IAA14-ARF7/19促进CWR (cell wall remodeling)基因的表达, 消融细胞壁, 从而使侧根原基突破内皮层并外露成形(Goh et al., 2012; Lavenus et al., 2013; Zhu et al., 2019). ...
MPK14- mediated auxin signaling controls lateral root development via ERF13-regulated very-long-chain fatty acid biosynthesis 2 2021
... 侧根形成过程不仅需要转录水平重编程来促进侧根细胞的分化成形, 也需要在时间和空间上对细胞形态和分裂进行有序调控, 必然存在细胞水平的非转录调控机制.山东大学丁兆军课题组最近发现, 生长素以不依赖于TIR1-AUX/IAA转录机制的方式调控侧根发育(Lv et al., 2021) (图1). ...
... 生长素激活MPK信号通路后如何进一步调控侧根发育? Lv等(2020)进一步的生化和遗传实验表明, MPK14直接与乙烯响应因子ERF13互作并磷酸化ERF13, 进而调控ERF13蛋白的稳定性.有意思的是, ERF13对侧根发育的调控部分依赖于超长链脂肪酸(very-long-chain fatty acids, VLCFAs)合成途径, 通过抑制VLCFA途径中β-酮脂酰辅酶A合成酶(KCS16)的转录水平来实现.VLCFA是一类由22及更多碳原子组成的脂肪酸, 是植物种子甘油三脂、表皮和表皮蜡质以及鞘脂的组成部分.前期研究表明, VLCFA在植物的抗逆性(Seo and Park, 2011)、花粉发育(Smirnova et al., 2013)、棉纤维细胞伸长(Qin et al., 2007)、生长素极性运输(Roudier et al., 2010)以及有丝分裂赤道板形成(Bach et al., 2011)等过程中发挥重要作用. ...
Organization and cell differentiation in lateral roots of Arabidopsis thaliana 1 1997
... 根系是植物固着生长的重要器官, 具有很强的可塑性, 以适应周边复杂且变化的环境.根系主要由主根和侧根组成.与主根不同, 侧根的形成属于胚后发育过程, 由中柱鞘细胞经命运转变而来.侧根的发育过程包括建成细胞的命运确定、侧根原基的形态建成以及侧根的成形(Malamy and Benfey, 1997; Péret et al., 2009; He and Meng, 2020).前期研究发现, 侧根发育的一系列过程都与生长素的时空分布有关, 其中侧根起始位置与生长素沿主根的节律性振荡有关, 而侧根发育又与局部生长素的有序累积有关(黎家和李传友, 2019).生长素通过TIR1/AFB-Aux/IAA介导的转录调控模式精准调控侧根发育的各个过程, 包括通过TRI1-IAA28-ARF5/6/7/8/19调控GATA23 (GA- TA-type transcription factor 23)基因的表达, 诱发侧根建成细胞的命运转变(De Rybel et al., 2010); 通过激活IAA14-ARF7/19和IAA12-ARF5协同影响中柱鞘细胞核的位移, 驱动早期侧根原基的不对称分裂(Hamann et al., 2002; Lee et al., 2009); 通过IAA3-ARF7和IAA14-ARF7/19促进CWR (cell wall remodeling)基因的表达, 消融细胞壁, 从而使侧根原基突破内皮层并外露成形(Goh et al., 2012; Lavenus et al., 2013; Zhu et al., 2019). ...
Emerging MAP kinase pathways in plant stress signaling 1 2005
... MAPK级联反应不仅参与调控植物细胞应对复杂多样的生物/非生物胁迫以及响应各种生理应激(Nakagami et al., 2005; Pitzschke et al., 2009), 而且几乎调节植物生长发育的各个方面: 从配子形成、胚胎发生、器官形态建成到器官脱落、植物衰老以及种子形成等(Xu and Zhang, 2015), 展现出MAPK信号通路在调控植物生长发育及响应整合外界环境信号中的强大功能. ...
Arabidopsis lateral root development: an emerging story 1 2009
... 根系是植物固着生长的重要器官, 具有很强的可塑性, 以适应周边复杂且变化的环境.根系主要由主根和侧根组成.与主根不同, 侧根的形成属于胚后发育过程, 由中柱鞘细胞经命运转变而来.侧根的发育过程包括建成细胞的命运确定、侧根原基的形态建成以及侧根的成形(Malamy and Benfey, 1997; Péret et al., 2009; He and Meng, 2020).前期研究发现, 侧根发育的一系列过程都与生长素的时空分布有关, 其中侧根起始位置与生长素沿主根的节律性振荡有关, 而侧根发育又与局部生长素的有序累积有关(黎家和李传友, 2019).生长素通过TIR1/AFB-Aux/IAA介导的转录调控模式精准调控侧根发育的各个过程, 包括通过TRI1-IAA28-ARF5/6/7/8/19调控GATA23 (GA- TA-type transcription factor 23)基因的表达, 诱发侧根建成细胞的命运转变(De Rybel et al., 2010); 通过激活IAA14-ARF7/19和IAA12-ARF5协同影响中柱鞘细胞核的位移, 驱动早期侧根原基的不对称分裂(Hamann et al., 2002; Lee et al., 2009); 通过IAA3-ARF7和IAA14-ARF7/19促进CWR (cell wall remodeling)基因的表达, 消融细胞壁, 从而使侧根原基突破内皮层并外露成形(Goh et al., 2012; Lavenus et al., 2013; Zhu et al., 2019). ...
MAPK cascade signaling networks in plant defence 1 2009
... MAPK级联反应不仅参与调控植物细胞应对复杂多样的生物/非生物胁迫以及响应各种生理应激(Nakagami et al., 2005; Pitzschke et al., 2009), 而且几乎调节植物生长发育的各个方面: 从配子形成、胚胎发生、器官形态建成到器官脱落、植物衰老以及种子形成等(Xu and Zhang, 2015), 展现出MAPK信号通路在调控植物生长发育及响应整合外界环境信号中的强大功能. ...
Saturated very-long-chain fatty acids promote cotton fiber and Arabidopsis cell elongation by activating ethylene biosynthesis 1 2007
... 生长素激活MPK信号通路后如何进一步调控侧根发育? Lv等(2020)进一步的生化和遗传实验表明, MPK14直接与乙烯响应因子ERF13互作并磷酸化ERF13, 进而调控ERF13蛋白的稳定性.有意思的是, ERF13对侧根发育的调控部分依赖于超长链脂肪酸(very-long-chain fatty acids, VLCFAs)合成途径, 通过抑制VLCFA途径中β-酮脂酰辅酶A合成酶(KCS16)的转录水平来实现.VLCFA是一类由22及更多碳原子组成的脂肪酸, 是植物种子甘油三脂、表皮和表皮蜡质以及鞘脂的组成部分.前期研究表明, VLCFA在植物的抗逆性(Seo and Park, 2011)、花粉发育(Smirnova et al., 2013)、棉纤维细胞伸长(Qin et al., 2007)、生长素极性运输(Roudier et al., 2010)以及有丝分裂赤道板形成(Bach et al., 2011)等过程中发挥重要作用. ...
Very-long-chain fatty acids are involved in polar auxin transport and developmental patterning in Arabidopsis 1 2010
... 生长素激活MPK信号通路后如何进一步调控侧根发育? Lv等(2020)进一步的生化和遗传实验表明, MPK14直接与乙烯响应因子ERF13互作并磷酸化ERF13, 进而调控ERF13蛋白的稳定性.有意思的是, ERF13对侧根发育的调控部分依赖于超长链脂肪酸(very-long-chain fatty acids, VLCFAs)合成途径, 通过抑制VLCFA途径中β-酮脂酰辅酶A合成酶(KCS16)的转录水平来实现.VLCFA是一类由22及更多碳原子组成的脂肪酸, 是植物种子甘油三脂、表皮和表皮蜡质以及鞘脂的组成部分.前期研究表明, VLCFA在植物的抗逆性(Seo and Park, 2011)、花粉发育(Smirnova et al., 2013)、棉纤维细胞伸长(Qin et al., 2007)、生长素极性运输(Roudier et al., 2010)以及有丝分裂赤道板形成(Bach et al., 2011)等过程中发挥重要作用. ...
Cuticular wax biosynthesis as a way of inducing drought resistance 1 2011
... 生长素激活MPK信号通路后如何进一步调控侧根发育? Lv等(2020)进一步的生化和遗传实验表明, MPK14直接与乙烯响应因子ERF13互作并磷酸化ERF13, 进而调控ERF13蛋白的稳定性.有意思的是, ERF13对侧根发育的调控部分依赖于超长链脂肪酸(very-long-chain fatty acids, VLCFAs)合成途径, 通过抑制VLCFA途径中β-酮脂酰辅酶A合成酶(KCS16)的转录水平来实现.VLCFA是一类由22及更多碳原子组成的脂肪酸, 是植物种子甘油三脂、表皮和表皮蜡质以及鞘脂的组成部分.前期研究表明, VLCFA在植物的抗逆性(Seo and Park, 2011)、花粉发育(Smirnova et al., 2013)、棉纤维细胞伸长(Qin et al., 2007)、生长素极性运输(Roudier et al., 2010)以及有丝分裂赤道板形成(Bach et al., 2011)等过程中发挥重要作用. ...
Very-long-chain fatty acids restrict regeneration capacity by confining pericycle competence for callus formation in Arabidopsis 1 2016
... VLCFA是细胞壁的重要组分(Bach and Faure, 2010).Lv等(2020)研究表明, VLCFA通过影响细胞壁成分果胶质的降解来限制侧根突破内皮层, 从而导致成形侧根数量减少, 而过量表达KCS16或外源施加VLCFA均可恢复ERF13过量表达植株中侧根突破障碍的缺陷表型, 最终证明VLCFA在植物侧根发育过程中具有重要作用.有意思的是, 胡玉欣课题组发现VLCFA及其衍生物作为细胞层特异的信号分子, 抑制植物中柱鞘类细胞命运转变, 从而调控愈伤组织的形成(Shang et al., 2016).在拟南芥中, 侧根的起始和愈伤组织的形成均源于中柱鞘细胞的转分化(Che et al., 2007; Atta et al., 2009; Sugimoto et al., 2010), 而VLCFA在这2个过程中都发挥至关重要的作用, 表明VLCFA可能参与植物细胞分化的重要过程. ...
Deficiency in a very-long-chain fatty acid β-ketoacyl-coenzyme a synthase of tomato impairs microgametogenesis and causes floral organ fusion 1 2013
... 生长素激活MPK信号通路后如何进一步调控侧根发育? Lv等(2020)进一步的生化和遗传实验表明, MPK14直接与乙烯响应因子ERF13互作并磷酸化ERF13, 进而调控ERF13蛋白的稳定性.有意思的是, ERF13对侧根发育的调控部分依赖于超长链脂肪酸(very-long-chain fatty acids, VLCFAs)合成途径, 通过抑制VLCFA途径中β-酮脂酰辅酶A合成酶(KCS16)的转录水平来实现.VLCFA是一类由22及更多碳原子组成的脂肪酸, 是植物种子甘油三脂、表皮和表皮蜡质以及鞘脂的组成部分.前期研究表明, VLCFA在植物的抗逆性(Seo and Park, 2011)、花粉发育(Smirnova et al., 2013)、棉纤维细胞伸长(Qin et al., 2007)、生长素极性运输(Roudier et al., 2010)以及有丝分裂赤道板形成(Bach et al., 2011)等过程中发挥重要作用. ...
Arabidopsis regeneration from multiple tissues occurs via a root development pathway 1 2010
... VLCFA是细胞壁的重要组分(Bach and Faure, 2010).Lv等(2020)研究表明, VLCFA通过影响细胞壁成分果胶质的降解来限制侧根突破内皮层, 从而导致成形侧根数量减少, 而过量表达KCS16或外源施加VLCFA均可恢复ERF13过量表达植株中侧根突破障碍的缺陷表型, 最终证明VLCFA在植物侧根发育过程中具有重要作用.有意思的是, 胡玉欣课题组发现VLCFA及其衍生物作为细胞层特异的信号分子, 抑制植物中柱鞘类细胞命运转变, 从而调控愈伤组织的形成(Shang et al., 2016).在拟南芥中, 侧根的起始和愈伤组织的形成均源于中柱鞘细胞的转分化(Che et al., 2007; Atta et al., 2009; Sugimoto et al., 2010), 而VLCFA在这2个过程中都发挥至关重要的作用, 表明VLCFA可能参与植物细胞分化的重要过程. ...
Mitogen-activated protein kinase cascades in signaling plant growth and development 1 2015
... MAPK级联反应不仅参与调控植物细胞应对复杂多样的生物/非生物胁迫以及响应各种生理应激(Nakagami et al., 2005; Pitzschke et al., 2009), 而且几乎调节植物生长发育的各个方面: 从配子形成、胚胎发生、器官形态建成到器官脱落、植物衰老以及种子形成等(Xu and Zhang, 2015), 展现出MAPK信号通路在调控植物生长发育及响应整合外界环境信号中的强大功能. ...
A MAPK cascade downstream of IDA-HAE/HSL2 ligand-receptor pair in lateral root emergence 2 2019
... 根系是植物固着生长的重要器官, 具有很强的可塑性, 以适应周边复杂且变化的环境.根系主要由主根和侧根组成.与主根不同, 侧根的形成属于胚后发育过程, 由中柱鞘细胞经命运转变而来.侧根的发育过程包括建成细胞的命运确定、侧根原基的形态建成以及侧根的成形(Malamy and Benfey, 1997; Péret et al., 2009; He and Meng, 2020).前期研究发现, 侧根发育的一系列过程都与生长素的时空分布有关, 其中侧根起始位置与生长素沿主根的节律性振荡有关, 而侧根发育又与局部生长素的有序累积有关(黎家和李传友, 2019).生长素通过TIR1/AFB-Aux/IAA介导的转录调控模式精准调控侧根发育的各个过程, 包括通过TRI1-IAA28-ARF5/6/7/8/19调控GATA23 (GA- TA-type transcription factor 23)基因的表达, 诱发侧根建成细胞的命运转变(De Rybel et al., 2010); 通过激活IAA14-ARF7/19和IAA12-ARF5协同影响中柱鞘细胞核的位移, 驱动早期侧根原基的不对称分裂(Hamann et al., 2002; Lee et al., 2009); 通过IAA3-ARF7和IAA14-ARF7/19促进CWR (cell wall remodeling)基因的表达, 消融细胞壁, 从而使侧根原基突破内皮层并外露成形(Goh et al., 2012; Lavenus et al., 2013; Zhu et al., 2019). ...
,*福建农林大学海峡联合研究院, 园艺植物生物学及代谢组学研究中心, 福州 350002
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