Abstract Wild soybeans (Glycine soja) originated in China, which was the closest ancestor of soybean. Because of the remarkable adaptability to adverse conditions, wild soybean has become an ideal material for the study of key genes in regulating stress tolerance. In this review, we provided an overview on the genome, transcriptome and proteome of wild soybean in stress tolerance. Meanwhile, we summarized current research progress on the protein kinases, transcription factors, ion channels and redox regulation in response to stress, which will provide new ideas for the cultivation of stress- tolerant crops in the future. Keywords：stress;ion channel;omics;protein kinase;redox;transcription factor;wild soybean
PDF (741KB)元数据多维度评价相关文章导出EndNote|Ris|Bibtex收藏本文 引用本文 王研, 贾博为, 孙明哲, 孙晓丽. 野生大豆耐逆分子调控机制研究进展. 植物学报, 2021, 56(1): 104-115 doi:10.11983/CBB20144 Wang Yan, Jia Bowei, Sun Mingzhe, Sun Xiaoli. Advances in Molecular Mechanisms of Stress Tolerance in Wild Soybean. Chinese Bulletin of Botany, 2021, 56(1): 104-115 doi:10.11983/CBB20144
蛋白质组分析技术与基因组和转录组测序技术相比起步较晚, 目前尚处于初期阶段。张宁(2015)以50 mmol·L-1 NaHCO3胁迫下野生大豆(G07256)叶片为材料, 利用双向凝胶电泳(two-dimensional electrophoresis, 2-DE)技术筛选出101个差异表达蛋白。转录组测序在碱处理1小时就能鉴定到差异表达基因, 而蛋白质组分析在24和48小时后得到的差异蛋白点最多。Ji等(2016a)采用同位素标记相对和绝对定量(isobaric tags for relative and absolute quantification, iTRAQ)技术分析了盐处理12小时大豆叶片和根的蛋白质组, 获得50个差异表达蛋白; 并分析了GsCBRLK过表达大豆和野生型叶片盐胁迫的蛋白质组, 获得了941个差异表达蛋白, 其中574个依赖GsCBRLK (Ji et al., 2016b)。Pi等(2016)分析了盐胁迫下大豆耐盐和盐敏感品种根的磷酸蛋白质组, 鉴定了1 163个差异磷酸化位点, 并发现磷酸化的MYB转录因子介导的查尔酮代谢途径参与盐胁迫应答。
虽然已有关于大豆在不同逆境胁迫下蛋白质组和磷酸化蛋白质组变化的报道, 但对野生大豆蛋白质组的研究却报道较少。鉴于转录水平差异并不能代表蛋白质水平差异(Hossain et al., 2013), 因此研究野生大豆逆境下的蛋白质组对揭示其对逆境胁迫的响应至关重要。相信随着蛋白质组技术的逐步完善, 会建立不同条件下更全面的蛋白表达谱, 以更直接地了解野生大豆逆境下的信号转导途径和耐逆分子机制。
2 蛋白激酶调控野生大豆的耐逆应答
蛋白激酶通过磷酸化下游靶蛋白, 启动或关闭信号转导通路, 调控植物逆境应答。大豆中4.67%的基因编码蛋白激酶(protein kinase), 其中约65%属于类受体蛋白激酶(receptor like kinases, RLKs) (Zulawski et al., 2014; Liu et al., 2015b)。RLKs蛋白一般包含1个胞外结构域、1个跨膜结构域和1个胞内激酶结构域。
AliZ, ZhangDY, XuZL, XuL, YiJX, HeXL, HuangYH, LiuXQ, KhanAA, TrethowanRM, MaHX (2012). Uncovering the salt response of soybean by unraveling its wild and cultivated functional genomes using tag sequencing PLoS One 7, e48819. DOI:10.1371/journal.pone.0048819URL [本文引用: 1]
BaiX, LiuJ, TangLL, CaiH, ChenM, JiW, LiuY, ZhuYM (2013). Overexpression of GsCBRLK from Glycine soja enhances tolerance to salt stress in transgenic alfalfa (Medicago sativa) Funct Plant Biol 40, 1048-1056. DOI:10.1071/FP12377URL [本文引用: 2] GsCBRLK encodes a novel plant-specific calcium-dependent calmodulin-binding receptor-like kinase from Glycine soja Siebold & Zucc. In our previous study, GsCBRLK was found to be a positive regulator of plant tolerance to salt and abscisic acid (ABA) stress. In this study we transformed alfalfa (Medicago sativa L.) with GsCBRLK to assess whether forage legumes overexpressing GsCBRLK adapt to saline soils. Results showed that transgenic alfalfa plants overexpressing GsCBRLK exhibited enhanced salt tolerance. Transgenic alfalfa grew well in the presence of 300 mM NaCl for 15 days, whereas wild-type (WT) plants exhibited severe chlorosis and growth retardation. Although transgenic alfalfa grew slowly and even had yellow leaves under the 400 mM NaCl treatment, most of the WT plants exhibited more severe chlorosis and did not survive. In addition, samples from transgenic and WT plants treated with 300 mM NaCl for 0, 3, 6, 9, 12, and 15 days were selected for physiological analysis. Lower membrane leakage and malondialdehyde (MDA) content were observed in transgenic alfalfa compared with WT plants during salt treatment. The reduction of chlorophyll content in transgenic alfalfa was less than that in WT plants. Furthermore, the plants that overexpressed GsCBRLK showed enhanced superoxide dismutase (SOD) activity, less of a Na+ increase, and a greater K+ decrease than WT plants. These results indicated that the overexpression of GsCBRLK confers enhanced tolerance to salt stress in transgenic alfalfa.
BianXH, LiW, NiuCF, WeiW, HuY, HanJQ, LuX, TaoJJ, JinM, QinH, ZhouB, ZhangWK, MaB, WangGD, YuDY, LaiYC, ChenSY, ZhangJS (2020). A class B heat shock factor selected for during soybean domestication contributes to salt tolerance by promoting flavonoid biosynthesis New Phytol 225, 268-283. URLPMID:31400247 [本文引用: 1]
CaoL, YuY, DingXD, ZhuD, YangF, LiuBD, SunXL, DuanXB, YinKD, ZhuYM (2017). The Glycine soja NAC transcription factor GsNAC019 mediates the regulation of plant alkaline tolerance and ABA sensitivity Plant Mol Biol 95, 253-268. URLPMID:28884328 [本文引用: 1]
CaoL, YuY, DuanmuH, ChenC, DuanXB, ZhuPH, ChenRR, LiQ, ZhuYM, DingXD (2016). A novel Glycine soja homeodomain-leucine zipper (HD-Zip) I gene, Gshdz4, positively regulates bicarbonate tolerance and responds to osmotic stress in Arabidopsis BMC Plant Biol 16, 184. DOI:10.1186/s12870-016-0872-7URLPMID:27553065 [本文引用: 1] BACKGROUND: Wild soybean (Glycine soja) is a highly adaptive plant species which can grow well in saline-alkaline soils. In soybean genome, there exist about 140 HD-Zip (Homeodomain-leucine Zipper) genes. HD-Zip transcription factor family is one of the largest plant specific superfamilies and plays important roles in response to abiotic stresses. Although HD-Zip transcription factors have been broadly reported to be involved in plant resistance to abiotic stresses like salt and drought, their roles in response to bicarbonate stress is largely unknown. RESULTS: From our previous transcriptome profile analysis of wild soybean treated by 50 mM NaHCO3, we identified an HD-Zip gene (Gshdz4) which showed high response to the alkaline stress. Our result of qRT-PCR showed that the expression of Gshdz4 was induced by alkaline stress (NaHCO3) in both leaves and roots of wild soybean. Overexpression of Gshdz4 in Arabidopsis resulted in enhanced tolerance to NaHCO3 and KHCO3 during the process of plant growth and development. However, the growths of transgenic and WT plants were not significantly different on the medium with high pH adjusted by KOH, implicating Gshdz4 is only responsible for resisting HCO3 (-) but not high pH. The transgenic plants had less MDA contents but higher POD activities and chlorophyll contents than the WT plants. Moreover, the transcript levels of stress-related genes, such as NADP-ME, H (+) -Ppase, RD29B and KIN1 were increased with greater extent in the transgenic plants than the wild plants. On the contrary, Gshdz4 overexpression lines were much sensitive to osmotic stress at seed germination and stocking stages compared to the wild plants. CONCLUSIONS: We revealed that the important and special roles of Gshdz4 in enhancing bicarbonate tolerance and responding to osmotic stress. It is the first time to elucidate these novel functions of HD-ZIP transcription factors. All the evidences broaden our understanding of functions of HD-Zip family and provide clues for uncovering the mechanisms of high tolerance of wild soybean to saline-alkaline stresses.
ChenC, SunXL, DuanmuH, YuY, LiuAL, XiaoJL, ZhuYM (2015). Ectopic expression of a Glycine soja myo- inositol oxygenase gene (GsMIOX1a) in Arabidopsis enhances tolerance to alkaline stress PLoS One 10, e0129-998. URLPMID:26720417 [本文引用: 3]
DuanXB, YangY, ZhangY, ChenC, DuanmuHZ, CaoL, SunMZ, SunXL, ZhuYM (2018a). A potential efflux boron transporter gene GsBOR2, positively regulates Arabidopsis bicarbonate tolerance Plant Sci 274, 284-292. DOI:10.1016/j.plantsci.2018.05.032URLPMID:30080614 [本文引用: 2] Soil alkalization severely restricts agricultural production and economic development worldwide, this problem is far more serious in Songnen Plain, the largest commodity grain base of China. However, little research has been done concerning the mechanisms of plant responses to alkaline stress to date. In this study, we isolated an alkali inducible gene GsBOR2 from Glycine soja on the basis of RNA seq data. GsBOR2 sh high protein sequence similarity with the known boron transporters in other species. The expression of GsBOR2 was highly up-regulated by 50mM NaHCO3 treatment and displayed tissue specificity. We then generated the transgenic Arabidopsis overexpressing GsBOR2 and found that the transgenic lines exhibited enhanced alkaline tolerance compared to wild type plants, as illustrated by longer roots and greater shoot biomass. Moreover, GsBOR2 overexpression was also capable of increasing plant resistance to KHCO3 treatment but not to high-pH stress. Functional complementation of Scbor1 mutant yeasts suggested that GsBOR2 could likely mediate the efflux of boron from cells. Taken together, the alkali responsive gene GsBOR2 is a positive regulator of plant bicarbonate tolerance.
DuanXB, YuY, DuanmuHZ, ChenC, SunXL, CaoL, LiQ, DingXD, LiuBD, ZhuYM (2018b). GsSLAH3, a Glycine soja slow type anion channel homolog, positively modulates plant bicarbonate stress tolerance Physiol Plant 164, 145-162. DOI:10.1111/ppl.12683URLPMID:29243826 [本文引用: 3] Alkaline stress is a major form of abiotic stress that severely inhibits plant growth and development, thus restricting crop productivity. However, little is known about how plants respond to alkali. In this study, a slow-type anion channel homolog 3 gene, GsSLAH3, was isolated and functionally characterized. Bioinformatics analysis showed that the GsSLAH3 protein contains 10 transmembrane helices. Consistently, GsSLAH3 was found to locate on plasma membrane by transient expression in onion epidermal cells. In wild soybeans, GsSLAH3 expression was induced by NaHCO3 treatment, suggesting its involvement in plant response to alkaline stress. Ectopic expression of GsSLAH3 in yeast increased sensitivity to alkali treatment. Dramatically, overexpression of GsSLAH3 in Arabidopsis thaliana enhanced alkaline tolerance during the germination, seedling and adult stages. More interestingly, we found that transgenic lines also improved plant tolerance to KHCO3 rather than high pH treatment. A nitrate content analysis of Arabidopsis shoots showed that GsSLAH3 overexpressing lines accumulated more NO3(-) than wild-type. In summary, our data suggest that GsSLAH3 is a positive alkali responsive gene that increases bicarbonate resistance specifically.
DuanmuHZ, WangY, BaiX, ChengSF, DeyholosMK, WongGKS, LiD, ZhuD, LiR, YuY, CaoL, ChenC, ZhuYM (2015). Wild soybean roots depend on specific transcription factors and oxidation reduction related genes in response to alkaline stress Funct Integr Genomics 15, 651-660. [本文引用: 1]
GeY, LiY, LvDK, BaiX, JiW, CaiH, WangAX, ZhuYM (2011). Alkaline-stress response in Glycine soja leaf identifies specific transcription factors and ABA-mediated signaling factors Funct Integr Genomics 11, 369-379. URLPMID:20938706 [本文引用: 2]
GeY, LiY, ZhuYM, BaiX, LvDK, GuoDJ, JiW, CaiH (2010). Global transcriptome profiling of wild soybean (Glycine soja) roots under NaHCO3 treatment BMC Plant Biol 10, 153. URLPMID:20653984 [本文引用: 2]
GurungPD, UpadhyayAK, BhardwajPK, SowdhaminiR, RamakrishnanU (2019). Transcriptome analysis reveals plasticity in gene regulation due to environmental cues in Primula sikkimensis, a high altitude plant species BMC Genomics 20, 989. DOI:10.1186/s12864-019-6354-1URLPMID:31847812 [本文引用: 1] BACKGROUND: Studying plasticity in gene expression in natural systems is crucial, for predicting and managing the effects of climate change on plant species. To understand the contribution of gene expression level variations to abiotic stress compensation in a Himalaya plant (Primula sikkimensis), we carried out a transplant experiment within (Ambient), and beyond (Below Ambient and Above Ambient) the altitudinal range limit of species. We sequenced nine transcriptomes (three each from each altitudinal range condition) using Illumina sequencing technology. We compared the fitness variation of transplants among three transplant conditions. RESULTS: A large number of significantly differentially expressed genes (DEGs) between below ambient versus ambient (109) and above ambient versus ambient (85) were identified. Transcripts involved in plant growth and development were mostly up-regulated in below ambient conditions. Transcripts involved in signalling, defence, and membrane transport were mostly up-regulated in above ambient condition. Pathway analysis revealed that most of the genes involved in metabolic processes, secondary metabolism, and flavonoid biosynthesis were differentially expressed in below ambient conditions, whereas most of the genes involved in photosynthesis and plant hormone signalling were differentially expressed in above ambient conditions. In addition, we observed higher reproductive fitness in transplant individuals at below ambient condition compared to above ambient conditions; contrary to what we expect from the cold adaptive P. sikkimensis plants. CONCLUSIONS: We reveal P. sikkimensis's capacity for rapid adaptation to climate change through transcriptome variation, which may facilitate the phenotypic plasticity observed in morphological and life history traits. The genes and pathways identified provide a genetic resource for understanding the temperature stress (both the hot and cold stress) tolerance mechanism of P. sikkimensis in their natural environment.
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JiW, CongR, LiS, LiR, QinZW, LiYJ, ZhouXL, ChenSX, LiJ (2016a). Comparative proteomic analysis of soybean leaves and roots by iTRAQ provides insights into response mechanisms to short-term salt stress Front Plant Sci 7, 573. URLPMID:27200046 [本文引用: 1]
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JiW, LiY, LiJ, DaiCH, WangX, BaiX, CaiH, YangL, ZhuYM (2006). Generation and analysis of expressed sequence tags from NaCl-treated Glycine soja BMC Plant Biol 6, 4. [本文引用: 1]
JiW, ZhuYM, LiY, YangL, ZhaoXW, CaiH, BaiX (2010). Over-expression of a glutathione S-transferase gene, GsGST, from wild soybean (Glycine soja) enhances drought and salt tolerance in transgenic tobacco Biotechnol Lett 32, 1173-1179. URLPMID:20383560 [本文引用: 2]
JiaBW, SunMZ, DuanmuHZ, DingXD, LiuBD, ZhuYM, SunXL (2017). GsCHX19.3, a member of cation/H+ exchanger superfamily from wild soybean contributes to high salinity and carbonate alkaline tolerance Sci Rep 7, 9423. [本文引用: 2]
JiaBW, SunMZ, SunXL, LiRT, WangZY, WuJ, WeiZW, DuanmuHZ, XiaoJL, ZhuYM (2016). Overexpression of GsGSTU13 and SCMRP in Medicago sativa confers increased salt-alkaline tolerance and methionine content Physiol Plant 156, 176-189. [本文引用: 2]
KimMYK, LeeS, VanK, KimTH, JeongSC, ChoiIY, KimDS, LeeYS, ParkD, MaJX, KimWY, KimBC, ParkS, LeeKA, KimDH, KimKH, ShinJH, JangYE, KimKD, LiuWX, ChaisanT, KangYJ, LeeYH, KimKH, MoonJK, SchmutzJ, JacksonSA, BhakJ, LeeSH (2010). Whole-genome sequencing and intensive analysis of the undomesticated soybean (Glycine soja Sieb. and Zucc.) genome Proc Natl Acad Sci USA 51, 22032-22037. [本文引用: 1]
LamHM, XuX, LiuX, ChenWB, YangGH, WongFL, LiMW, HeWM, QinN, WangB, LiJ, JianM, WangJ, ShaoGH, WangJ, SunSSM, ZhangGY (2010). Resequencing of 31 wild and cultivated soybean genomes identifies patterns of genetic diversity and selection Nat Genet 42, 1053-1059. DOI:10.1038/ng.715URLPMID:21076406 [本文引用: 1] We report a large-scale analysis of the patterns of genome-wide genetic variation in soybeans. We re-sequenced a total of 17 wild and 14 cultivated soybean genomes to an average of approximately x5 depth and >90% coverage using the Illumina Genome Analyzer II platform. We compared the patterns of genetic variation between wild and cultivated soybeans and identified higher allelic diversity in wild soybeans. We identified a high level of linkage disequilibrium in the soybean genome, suggesting that marker-assisted breeding of soybean will be less challenging than map-based cloning. We report linkage disequilibrium block location and distribution, and we identified a set of 205,614 tag SNPs that may be useful for QTL mapping and association studies. The data here provide a valuable resource for the analysis of wild soybeans and to facilitate future breeding and quantitative trait analysis.
LiXJ, WangY, LiuF, PiBY, ZhaoTJ, YuBJ (2020). Transcriptomic analysis of Glycine soja and G. max seedlings and functional characterization of GsGSTU24 and GsGSTU42 genes under submergence stress Environ Exp Bot 171, 103963. [本文引用: 2]
LiYH, ZhouGY, MaJX, JiangWK, JinLG, ZhangZH, GuoY, ZhangJB, SuiY, ZhengLT, ZhangSS, ZuoQY, ShiXH, LiYF, ZhangWK, HuYY, KongGY, HongHL, TanB, SongJ, LiuZX, WangYS, RuanH, YeungCKL, LiuJ, WangHL, ZhangLJ, GuanRX, WangKJ, LiWB, ChenSY, ChangRZ, JiangZ, JacksonSA, LiRQ, QiuLJ (2014). De novo assembly of soybean wild relatives for pan-genome analysis of diversity and agronomic traits Nat Biotechnol 32, 1045-1052. [本文引用: 1]
LiuAL, YuY, DuanXB, SunXL, DuanmuHZ, ZhuYM (2015a). GsSKP21, a Glycine soja S-phase kinase-associated protein, mediates the regulation of plant alkaline tolerance and ABA sensitivity Plant Mol Biol 87, 111-124. DOI:10.1007/s11103-014-0264-zURLPMID:25477077 Plant SKP1-like family proteins, components of the SCF complex E3 ligases, are involved in the regulation of plant development and stress responses. Little is known about the precise function of SKP genes in plant responses to environmental stresses. GsSKP21 was initially identified as a potential stress-responsive gene based on the transcriptome sequencing of Glycine soja. In this study, we found that GsSKP21 protein contains highly conserved SKP domains in its N terminus and an extra unidentified domain in its C terminus. The transcript abundance of GsSKP21, detected by quantitative real-time PCR, was induced under the treatment of alkali and salt stresses. Overexpression of GsSKP21 in Arabidopsis dramatically increased plant tolerance to alkali stress. Furthermore, we found that overexpression of GsSKP21 resulted in decreased ABA sensitivity during both the seed germination and early seedling growth stages. GsSKP21 mediated ABA signaling by altering the expression levels of the ABA signaling-related and ABA-induced genes. We also investigated the tissue expression specificity and subcellular localization of GsSKP21. These results suggest that GsSKP21 is important for plant tolerance to alkali stress and plays a critical regulatory role in the ABA-mediated stress response.
LiuJY, ChenNN, GrantJN, ChengZM, Stewart CNJr, HeweziT (2015b). Soybean kinome: functional classification and gene expression patterns J Exp Bot 66, 1919-1934. DOI:10.1093/jxb/eru537URLPMID:25614662 [本文引用: 2] The protein kinase (PK) gene family is one of the largest and most highly conserved gene families in plants and plays a role in nearly all biological functions. While a large number of genes have been predicted to encode PKs in soybean, a comprehensive functional classification and global analysis of expression patterns of this large gene family is lacking. In this study, we identified the entire soybean PK repertoire or kinome, which comprised 2166 putative PK genes, representing 4.67% of all soybean protein-coding genes. The soybean kinome was classified into 19 groups, 81 families, and 122 subfamilies. The receptor-like kinase (RLK) group was remarkably large, containing 1418 genes. Collinearity analysis indicated that whole-genome segmental duplication events may have played a key role in the expansion of the soybean kinome, whereas tandem duplications might have contributed to the expansion of specific subfamilies. Gene structure, subcellular localization prediction, and gene expression patterns indicated extensive functional divergence of PK subfamilies. Global gene expression analysis of soybean PK subfamilies revealed tissue- and stress-specific expression patterns, implying regulatory functions over a wide range of developmental and physiological processes. In addition, tissue and stress co-expression network analysis uncovered specific subfamilies with narrow or wide interconnected relationships, indicative of their association with particular or broad signalling pathways, respectively. Taken together, our analyses provide a foundation for further functional studies to reveal the biological and molecular functions of PKs in soybean.
LuoX, BaiX, ZhuD, LiY, JiW, CaiH, WuJ, LiuBH, ZhuYM (2012a). GsZFP1, a new Cys2/His2-type zinc-finger protein, is a positive regulator of plant tolerance to cold and drought stress Planta 235, 1141-1155. DOI:10.1007/s00425-011-1563-0URLPMID:22160567 [本文引用: 4] Plant acclimation to environmental stress is controlled by a complex network of regulatory genes that compose distinct stress-response regulons. The C2H2-type zinc-finger proteins (ZFPs) have been implicated in different cellular processes involved in plant development and stress responses. Through microarray analysis, an alkaline (NaHCO(3))-responsive ZFP gene GsZFP1 was identified and subsequently cloned from Glyycine soja. GsZFP1 encodes a 35.14 kDa protein with one C2H2-type zinc-finger motif. The QALGGH domain, conserved in most plant C2H2-type ZFPs, is absent in the GsZFP1 protein sequence. A subcellular localization study using a GFP fusion protein indicated that GsZFP1 is localized to the nucleus. Real-time RT-PCR analysis showed that GsZFP1 was induced in the leaf by ABA (100 muM), salt (200 mM NaCl), and cold (4 degrees C), and in the root by ABA (100 muM), cold (4 degrees C), and drought (30% PEG 6000). Over-expression of GsZFP1 in transgenic Arabidopsis resulted in a greater tolerance to cold and drought stress, a decreased water loss rate, and an increase in proline irrespective of environmental conditions. The over-expression of GsZFP1 also increased the expression of a number of stress-response marker genes, including CBF1, CBF2, CBF3, NCED3, COR47, and RD29A in response to cold stress and RAB18, NCED3, P5CS, RD22, and RD29A in response to drought stress, especially early during stress treatments. Our studies suggest that GsZFP1 plays a crucial role in the plant response to cold and drought stress.
LuoX, CuiN, ZhuYM, CaoL, ZhaiH, CaiH, JiW, WangXD, ZhuD, LiY, BaiX (2012b). Over-expression of GsZFP1, an ABA-responsive C2H2-type zinc finger protein lacking a QALGGH motif, reduces ABA sensitivity and decreases stomata size J Plant Physiol 169, 1192-1202. DOI:10.1016/j.jplph.2012.03.019URLPMID:22705253 [本文引用: 2] A cDNA of the gene GsZFP1 was cloned from Glycine soja. GsZFP1 encodes a protein with one C2H2-type zinc finger motif. The QALGGH motif, which exists in most plant C2H2-type zinc finger proteins (ZFPs), does not exist in GsZFP1. Real-time RT-PCR revealed that GsZFP1 expression was significantly up-regulated by exogenous ABA, both in leaves and roots. Over-expression of this gene, in Arabidopsis thaliana, resulted in a reduced sensitivity to ABA during seed germination and seedling growth. Transcript levels of some stress and ABA marker genes, including RD29A, RD22, NCED3, COR47, COR15A and KIN1 were increased, in the GsZFP1 over-expression lines, when plants were treated with exogenous ABA. We further studied the effects of GsZFP1 over-expression on the regulation of genes involved in ABA signaling. Negative ABA signaling regulators, such as ABI1 and ABI2, were up-regulated in over-expression lines, while positive ABA signaling regulators, such as ABF4, ABI5, GTG1, GTG2, PYR1/RCAR11, PYL2/RCAR13, SnRK2.2 and SnRK2.3, were down-regulated, in comparison to wild type plants. GsZFP1 over-expression lines also exhibited small stomata, impairment of ABA-induced stomata closure. The data presented, herein, suggests that GsZFP1 plays a crucial role in ABA signaling in A. thaliana, GsZFP1 may be a promising gene for negative regulating ABA signaling. Our findings broaden our understanding of this C2H2 ZFP subtype's function, and add to the body of evidence that has been developed in earlier studies.
LuoX, SunXL, LiuBH, ZhuD, XiB, CaiH, JiW, CaoL, WuJ, WangMC, DingXD, ZhuYM (2013a). Ectopic expression of a WRKY homolog from Glycine soja alters flowering time in Arabidopsis PLoS One 8, e73295. [本文引用: 1]
LuoX, XiB, SunXL, ZhuD, LiuBH, JiW, CaiH, CaoL, WuJ, HuMR, LiuX, TangLL, ZhuYM (2013b). Expression of wild soybean WRKY20 in Arabidopsis enhances drought tolerance and regulates ABA signaling J Exp Bot 8, 2155-2169. [本文引用: 2]
PiEX, QuLQ, HuJW, HuangYY, QiuLJ, LuHF, JiangB, LiuC, PengTT, ZhaoY, WangHZ, TsaiSN, NgaiS, DuLQ (2016). Mechanisms of soybean roots’ tolerances to salinity revealed by proteomic and phosphoproteomic comparisons between two cultivars Mol Cell Proteomics 15, 266-288. DOI:10.1074/mcp.M115.051961URLPMID:26407991 [本文引用: 1] Understanding molecular mechanisms underlying plant salinity tolerance provides valuable knowledgebase for effective crop improvement through genetic engineering. Current proteomic technologies, which support reliable and high-throughput analyses, have been broadly used for exploring sophisticated molecular networks in plants. In the current study, we compared phosphoproteomic and proteomic changes in roots of different soybean seedlings of a salt-tolerant cultivar (Wenfeng07) and a salt-sensitive cultivar (Union85140) induced by salt stress. The root samples of Wenfeng07 and Union85140 at three-trifoliate stage were collected at 0 h, 0.5 h, 1 h, 4 h, 12 h, 24 h, and 48 h after been treated with 150 mm NaCl. LC-MS/MS based phosphoproteomic analysis of these samples identified a total of 2692 phosphoproteins and 5509 phosphorylation sites. Of these, 2344 phosphoproteins containing 3744 phosphorylation sites were quantitatively analyzed. Our results showed that 1163 phosphorylation sites were differentially phosphorylated in the two compared cultivars. Among them, 10 MYB/MYB transcription factor like proteins were identified with fluctuating phosphorylation modifications at different time points, indicating that their crucial roles in regulating flavonol accumulation might be mediated by phosphorylated modifications. In addition, the protein expression profiles of these two cultivars were compared using LC MS/MS based shotgun proteomic analysis, and expression pattern of all the 89 differentially expressed proteins were independently confirmed by qRT-PCR. Interestingly, the enzymes involved in chalcone metabolic pathway exhibited positive correlations with salt tolerance. We confirmed the functional relevance of chalcone synthase, chalcone isomerase, and cytochrome P450 monooxygenase genes using soybean composites and Arabidopsis thaliana mutants, and found that their salt tolerance were positively regulated by chalcone synthase, but was negatively regulated by chalcone isomerase and cytochrome P450 monooxygenase. A novel salt tolerance pathway involving chalcone metabolism, mostly mediated by phosphorylated MYB transcription factors, was proposed based on our findings. (The mass spectrometry raw data are available via ProteomeXchange with identifier PXD002856).
SchmutzJ, CannonSB, SchlueterJ, MaJX, MitrosT, NelsonW, HytenDL, SongQJ, ThelenJJ, ChengJL, XuD, HellstenU, MayGD, YuY, SakuraiT, UmezawaT, BhattacharyyaMK, SandhuD, ValliyodanB, LindquistE, PetoM, GrantD, ShuSQ, GoodsteinD, BarryK, Futrell-GriggsM, AbernathyB, DuJC, TianZX, ZhuLC, GillN, JoshiT, LibaultM, SethuramanA, ZhangXC, ShinozakiK, NguyenHT, WingRA, CreganP, SpechtJ, GrimwoodJ, RokhsarD, StaceyG, ShoemakerRC, JacksonSA (2010). Genome sequence of the palaeopolyploid soybean Nature 463, 178-183. DOI:10.1038/nature08670URLPMID:20075913 [本文引用: 1] Soybean (Glycine max) is one of the most important crop plants for seed protein and oil content, and for its capacity to fix atmospheric nitrogen through symbioses with soil-borne microorganisms. We sequenced the 1.1-gigabase genome by a whole-genome shotgun approach and integrated it with physical and high-density genetic maps to create a chromosome-scale draft sequence assembly. We predict 46,430 protein-coding genes, 70% more than Arabidopsis and similar to the poplar genome which, like soybean, is an ancient polyploid (palaeopolyploid). About 78% of the predicted genes occur in chromosome ends, which comprise less than one-half of the genome but account for nearly all of the genetic recombination. Genome duplications occurred at approximately 59 and 13 million years ago, resulting in a highly duplicated genome with nearly 75% of the genes present in multiple copies. The two duplication events were followed by gene diversification and loss, and numerous chromosome rearrangements. An accurate soybean genome sequence will facilitate the identification of the genetic basis of many soybean traits, and accelerate the creation of improved soybean varieties.
ShenXJ, WangYY, ZhangYX, GuoW, JiaoYQ, ZhouXA (2018). Overexpression of the wild soybean R2R3-MYB transcription factor GsMYB15 enhances resistance to salt stress and Helicoverpa armigera in transgenic Arabidopsis Int J Mol Sci 19, 3958. [本文引用: 1]
SunMZ, JiaBW, CuiN, WenYD, DuanmuHZ, YuQY, XiaoJL, SunXL, ZhuYM (2016a). Functional characterization of a Glycine soja Ca2+ ATPase in salt-alkaline stress responses Plant Mol Biol 90, 419-434. DOI:10.1007/s11103-015-0426-7URLPMID:26801329 [本文引用: 2] It is widely accepted that Ca(2+)ATPase family proteins play important roles in plant environmental stress responses. However, up to now, most researches are limited in the reference plants Arabidopsis and rice. The function of Ca(2+)ATPases from non-reference plants was rarely reported, especially its regulatory role in carbonate alkaline stress responses. Hence, in this study, we identified the P-type II Ca(2+)ATPase family genes in soybean genome, determined their chromosomal location and gene architecture, and analyzed their amino acid sequence and evolutionary relationship. Based on above results, we pointed out the existence of gene duplication for soybean Ca(2+)ATPases. Then, we investigated the expression profiles of the ACA subfamily genes in wild soybean (Glycine soja) under carbonate alkaline stress, and functionally characterized one representative gene GsACA1 by using transgenic alfalfa. Our results suggested that GsACA1 overexpression in alfalfa obviously increased plant tolerance to both carbonate alkaline and neutral salt stresses, as evidenced by lower levels of membrane permeability and MDA content, but higher levels of SOD activity, proline concentration and chlorophyll content under stress conditions. Taken together, for the first time, we reported a P-type II Ca(2+)ATPase from wild soybean, GsACA1, which could positively regulate plant tolerance to both carbonate alkaline and neutral salt stresses.
SunMZ, QianX, ChenC, ChengSF, JiaBW, ZhuYM, SunXL (2018). Ectopic expression of GsSRK in Medicago sativa reveals its involvement in plant architecture and salt stress responses Front Plant Sci 9, 226. DOI:10.3389/fpls.2018.00226URLPMID:29520291 [本文引用: 1] Receptor-like kinases (RLK) play fundamental roles in plant growth and stress responses. Compared with other RLKs, little information is provided concerning the S-locus LecRLK subfamily, which is characterized by an extracellular G-type lectin domain and an S-locus-glycop domain. Until now, the function of the G-type lectin domain is still unknown. In a previous research, we identified a Glycine soja S-locus LecRLK gene GsSRK, which conferred increased salt stress tolerance in transgenic Arabidopsis. In this study, to investigate the role of the G-type lectin domain and to breed transgenic alfalfa with superior salt stress tolerance, we transformed the full-length GsSRK (GsSRK-f) and a truncated version of GsSRK (GsSRK-t) deleting the G-type lectin domain into alfalfa. Our results showed that overexpression of GsSRK-t, but not GsSRK-f, resulted in changes of plant architecture, as evidenced by more branches but shorter shoots of GsSRK-t transgenic alfalfa, indicating a potential role of the extracellular G-type lectin domain in regulating plant architecture. Furthermore, we also found that transgenic alfalfa overexpressing either GsSRK-f or GsSRK-t showed increased salt stress tolerance, and GsSRK-t transgenic alfalfa displayed better growth (more branches and higher fresh weight) than GsSRK-f lines under salt stress. In addition, our results suggested that both GsSRK-f and GsSRK-t were involved in ion homeostasis, ROS scavenging, and osmotic regulation. Under salt stress, the Na(+) content in the transgenic lines was significantly lower, while the K(+) content was slightly higher than that in WT. Moreover, the transgenic lines displayed reduced ion leakage and MDA content, but increased SOD activity and proline content than WT. Notably, no obvious difference in these physiological indices was observed between GsSRK-f and GsSRK-t transgenic lines, implying that deletion of the GsSRK G-type lectin domain does not affect its physiological function in salt stress responses. In conclusion, results in this research reveal the dual role of GsSRK in regulating both plant architecture and salt stress responses.
SunMZ, ShenY, YinKD, GuoYX, CaiXX, YangJK, ZhuYM, JiaBW, SunXL (2019). A late embryogenesis abundant protein GsPM30 interacts with a receptor like cytoplasmic kinase GsCBRLK and regulates environmental stress responses Plant Sci 283, 70-82. [本文引用: 2]
SunMZ, SunXL, ZhaoY, ZhaoCY, DuanmuHZ, YuY, JiW, ZhuYM (2014a). Ectopic expression of GsPPCK3 and SCMRP in Medicago sativa enhances plant alkaline stress tolerance and methionine content PLoS One 9, e89578. DOI:10.1371/journal.pone.0089578URLPMID:24586886 [本文引用: 2] So far, it has been suggested that phosphoenolpyruvate carboxylases (PEPCs) and PEPC kinases (PPCKs) fulfill several important non-photosynthetic functions. However, the biological functions of soybean PPCKs, especially in alkali stress response, are not yet well known. In previous studies, we constructed a Glycine soja transcriptional profile, and identified three PPCK genes (GsPPCK1, GsPPCK2 and GsPPCK3) as potential alkali stress responsive genes. In this study, we confirmed the induced expression of GsPPCK3 under alkali stress and investigated its tissue expression specificity by using quantitative real-time PCR analysis. Then we ectopically expressed GsPPCK3 in Medicago sativa and found that GsPPCK3 overexpression improved plant alkali tolerance, as evidenced by lower levels of relative ion leakage and MDA content and higher levels of chlorophyll content and root activity. In this respect, we further co-transformed the GsPPCK3 and SCMRP genes into alfalfa, and demonstrated the increased alkali tolerance of GsPPCK3-SCMRP transgenic lines. Further investigation revealed that GsPPCK3-SCMRP co-overexpression promoted the PEPC activity, net photosynthetic rate and citric acid content of transgenic alfalfa under alkali stress. Moreover, we also observed the up-regulated expression of PEPC, CS (citrate synthase), H(+)-ATPase and NADP-ME genes in GsPPCK3-SCMRP transgenic alfalfa under alkali stress. As expected, we demonstrated that GsPPCK3-SCMRP transgenic lines displayed higher methionine content than wild type alfalfa. Taken together, results presented in this study supported the positive role of GsPPCK3 in plant response to alkali stress, and provided an effective way to simultaneously improve plant alkaline tolerance and methionine content, at least in legume crops.
SunXL, CaiXX, YinKD, GuLW, ShenY, HuBS, WangY, ChenY, ZhuYM, JiaBW, SunMZ (2021). Wild soybean SNARE proteins BET1s mediate the subcellular localization of the cytoplasmic receptor-like kinases CRCK1s to modulate salt stress responses Plant J 105, 771-785. DOI:10.1111/tpj.15072URLPMID:33160290 [本文引用: 2] Plants have evolved numerous receptor-like kinases (RLKs) that modulate environmental stress responses. However, little is known regarding soybean (Glycine max) RLKs. We have previously identified that Glycine soja Ca(2+) /CAM-binding RLK (GsCBRLK) is involved in salt tolerance. Here, we report that soluble NSF attachment protein receptor proteins BET1s mediate subcellular localization of calmodulin-binding receptor-like cytoplasmic kinases CRCK1s to modulate salt stress responses. Direct interaction between GsCBRLK and GsBET11a was initially identified via yeast two-hybrid and bimolecular fluorescence complementation assays. Further analysis demonstrated conserved interaction between BET1s and CRCK1s. GsCBRLK interacted with all BET1 proteins in wild soybean (Glycine soja) and Arabidopsis, and GsBET11a strongly associated with GsCRCK1a-1d, but slightly with AtCRCK1. In addition, GsBET11a interacted with GsCBRLK via its C-terminal transmembrane domain (TMD), where the entire TMD, not the sequence, was critical for the interaction. Moreover, the N-terminal variable domain (VD) of GsCBRLK was responsible for interacting with GsBET11a, and the intensity of interaction between GsCBRLK/AtCRCK1 and GsBET11a was dependent on VD. Furthermore, GsBET11a was able to mediate the GsCBRLK subcellular localization via direct interaction with VD. Additionally, knockout of AtBET11 or AtBET12 individually did not alter GsCBRLK localization, while GsBET11a expression caused partial internalization of GsCBRLK from the plasma membrane (PM). We further suggest the necessity of GsCBRLK VD for its PM localization via N-terminal truncation assays. Finally, GsBET11a was shown to confer enhanced salt stress tolerance when overexpressed in Arabidopsis and soybean. These results revealed the conserved and direct interaction between BET1s and CRCK1s, and suggested their involvement in salt stress responses.
SunXL, SunMZ, JiaBW, QinZW, YangKJ, ChenC, YuQY, ZhuYM (2016b). A Glycine soja methionine sulfoxide reductase B5a interacts with the Ca2+/CAM-binding kinase GsCBRLK and activates ROS signaling under carbonate alkaline stress Plant J 86, 514-529. DOI:10.1111/tpj.13187URLPMID:27121031 [本文引用: 2] Although research has extensively illustrated the molecular basis of plant responses to salt and high-pH stresses, knowledge on carbonate alkaline stress is poor and the specific responsive mechanism remains elusive. We have previously characterized a Glycine soja Ca(2+) /CAM-dependent kinase GsCBRLK that could increase salt tolerance. Here, we characterize a methionine sulfoxide reductase (MSR) B protein GsMSRB5a as a GsCBRLK interactor by using Y2H and BiFc assays. Further analyses showed that the N-terminal variable domain of GsCBRLK contributed to the GsMSRB5a interaction. Y2H assays also revealed the interaction specificity of GsCBRLK with the wild soybean MSRB subfamily proteins, and determined that the BoxI/BoxII-containing regions within GsMSRBs were responsible for their interaction. Furthermore, we also illustrated that the N-terminal basic regions in GsMSRBs functioned as transit peptides, which targeted themselves into chloroplasts and thereby prevented their interaction with GsCBRLK. Nevertheless, deletion of these regions allowed them to localize on the plasma membrane (PM) and interact with GsCBRLK. In addition, we also showed that GsMSRB5a and GsCBRLK displayed overlapping tissue expression specificity and coincident expression patterns under carbonate alkaline stress. Phenotypic experiments demonstrated that GsMSRB5a and GsCBRLK overexpression in Arabidopsis enhanced carbonate alkaline stress tolerance. Further investigations elucidated that GsMSRB5a and GsCBRLK inhibited reactive oxygen species (ROS) accumulation by modifying the expression of ROS signaling, biosynthesis and scavenging genes. Summarily, our results demonstrated that GsCBRLK and GsMSRB5a interacted with each other, and activated ROS signaling under carbonate alkaline stress.
SunXL, SunMZ, LuoX, DingXD, JiW, CaiH, BaiX, LiuXF, ZhuYM (2013a). A Glycine soja ABA-responsive receptor-like cytoplasmic kinase, GsRLCK, positively controls plant tolerance to salt and drought stresses Planta 237, 1527-1545. DOI:10.1007/s00425-013-1864-6URLPMID:23494614 [本文引用: 2] Receptor such as protein kinases are proposed to work as sensors to initiate signaling cascades in higher plants. However, little is known about the precise functions of receptor such as protein kinases in abiotic stress response in plants, especially in wild soybean. Here, we focused on characterization of the biological functions of a receptor-like cytoplasmic serine/threonine protein kinase gene, GsRLCK, which was previously identified as a putative salt-alkali stress-related gene from the transcriptome profiles of Glycine soja. Bioinformatic analysis showed that GsRLCK protein contained a conserved kinase catalytic domain and two transmembrane domains at the N-terminus, but no typical extracellular domain. Consistently, GsRLCK-eGFP fusion protein was observed on the plasma membrane, but eGFP alone was distributing throughout the cytoplasm in onion epidermal cells. Quantitative real-time PCR analysis revealed the induced expression of GsRLCK by ABA, salt, alkali, and drought stresses. However, the expression levels of GsRLCK seemed to be similar in different tissues, except soybean pod. Phenotypic assays demonstrated that GsRLCK overexpression decreased ABA sensitivity and altered expression levels of ABA-responsive genes. Furthermore, we also found that GsRLCK conferred increased tolerance to salt and drought stresses and increased expression levels of a handful of stress-responsive genes, when overexpressing in Arabidopsis. In a word, we gave exact evidence that GsRLCK was a novel receptor-like cytoplasmic protein kinase and played a crucial role in plant responses to ABA, salt, and drought stresses.
SunXL, YangSS, SunMZ, WangST, DingXD, ZhuD, JiW, CaiH, ZhaoCY, WangXD, ZhuYM (2014b). A novel Glycine soja cysteine proteinase inhibitor GsCPI14, interacting with the calcium/calmodulin-binding receptor-like kinase GsCBRLK, regulated plant tolerance to alkali stress Plant Mol Biol 85, 33-48. DOI:10.1007/s11103-013-0167-4URLPMID:24407891 [本文引用: 2] It has been well demonstrated that cystatins regulated plant stress tolerance through inhibiting the cysteine proteinase activity under environmental stress. However, there was limited information about the role of cystatins in plant alkali stress response, especially in wild soybean. Here, in this study, we focused on the biological characterization of a novel Glycine soja cystatin protein GsCPI14, which interacted with the calcium/calmodulin-binding receptor-like kinase GsCBRLK and positively regulated plant alkali stress tolerance. The protein-protein interaction between GsCBRLK and GsCPI14 was confirmed by using split-ubiquitin based membrane yeast two-hybrid analysis and bimolecular fluorescence complementation assay. Expression of GsCPI14 was greatly induced by salt, ABA and alkali stress in G. soja, and GsCBRLK overexpression (OX) in Glycine max promoted the stress induction of GmCPI14 expression under stress conditions. Furthermore, we found that GsCPI14-eGFP fusion protein localized in the entire Arabidopsis protoplast and onion epidermal cell, and GsCPI14 showed ubiquitous expression in different tissues of G. soja. In addition, we gave evidence that the GST-GsCPI14 fusion protein inhibited the proteolytic activity of papain in vitro. At last, we demonstrated that OX of GsCPI14 in Arabidopsis promoted the seed germination under alkali stress, as evidenced by higher germination rates. GsCPI14 transgenic Arabidopsis seedlings also displayed better growth performance and physiological index under alkali stress. Taken together, results presented in this study demonstrated that the G. soja cysteine proteinase inhibitor GsCPI14 interacted with the calcium/calmodulin-binding receptor-like kinase GsCBRLK and regulated plant tolerance to alkali stress.
SunXL, YuQY, TangLL, JiW, BaiX, CaiH, LiuXF, DingXD, ZhuYM (2013b). GsSRK, a G-type lectin S-receptor- like serine/threonine protein kinase, is a positive regulator of plant tolerance to salt stress J Plant Physiol 170, 505-515. DOI:10.1016/j.jplph.2012.11.017URLPMID:23276523 [本文引用: 2] Receptor-like protein kinases (RLKs) play vital roles in sensing outside signals, yet little is known about RLKs functions and roles in stress signal perception and transduction in plants, especially in wild soybean. Through the microarray analysis, GsSRK was identified as an alkaline (NaHCO3)-responsive gene, and was subsequently isolated from Glycine soja by homologous cloning. GsSRK encodes a 93.22kDa protein with a highly conserved serine/threonine protein kinase catalytic domain, a G-type lectin region, and an S-locus region. Real-time PCR results showed that the expression levels of GsSRK were largely induced by ABA, salt, and drought stresses. Over expression of GsSRK in Arabidopsis promoted seed germination, as well as primary root and rosette leaf growth during the early stages of salt stress. Compared to the wild type Arabidopsis, GsSRK overexpressors exhibited enhanced salt tolerance and higher yields under salt stress, with higher chlorophyll content, lower ion leakage, higher plant height, and more siliques at the adult developmental stage. Our studies suggest that GsSRK plays a crucial role in plant response to salt stress.
TangLL, CaiH, JiW, LuoX, WangZY, WuJ, WangXD, CuiL, WangY, ZhuYM, BaiX (2013). Overexpression of GsZFP1 enhances salt and drought tolerance in transgenic alfalfa (Medicago sativa L.) Plant Physiol Biochem 71, 22-30. URLPMID:23867600 [本文引用: 3]
WangZY, SongFB, CaiH, ZhuYM, BaiX, JiW, LiY, HuaY (2012). Over-expressing GsGST14 from Glycine soja enhances alkaline tolerance of transgenic Medicago sativa Biol Plant 56, 516-520. [本文引用: 1]
WeiSS, WangXY, JiangD, DongST (2018). Physiological and proteome studies of maize (Z ea mays L.) in response to leaf removal under high plant density BMC Plant Biol 18, 378. [本文引用: 1]
WuSY, ZhuPH, JiaBW, YangJK, ShenY, CaiXX, SunXL, ZhuYM, SunMZ (2018). A Glycine soja group S2 bZIP transcription factor GsbZIP67 conferred bicarbonate alkaline tolerance in Medicago sativa BMC Plant Biol 18, 234. DOI:10.1186/s12870-018-1466-3URLPMID:30316294 [本文引用: 1] BACKGROUND: Even though bicarbonate alkaline stress is a serious threat to crop growth and yields, it attracts much fewer researches than high salinity stress. The basic leucine zipper (bZIP) transcription factors have been well demonstrated to function in diverse abiotic stresses; however, their biological role in alkaline tolerance still remains elusive. In this study, we functionally characterized a bZIP gene from Glycine soja GsbZIP67 in bicarbonate alkaline stress responses. RESULTS: GsbZIP67 was initially identified as a putative bicarbonate responsive gene, on the basis of previous RNA-seq data of 50 mM NaHCO3-treated Glycine soja roots. GsbZIP67 protein possessed a conserved bZIP domain, and belonged to the group S2 bZIP, which is yet less well-studied. Our studies showed that GsbZIP67 targeted to nucleus in Arabidopsis protoplasts, and displayed transcriptional activation activity in yeast cells. The quantitative real-time PCR analyses unraveled the bicarbonate stress responsive expression and tissue specific expression of GsbZIP67 in wild soybean. Further phenotypic analysis illustrated that GsbZIP67 overexpression in alfalfa promoted plant growth under bicarbonate alkaline stress, as evidenced by longer roots and shoots. Furthermore, GsbZIP67 overexpression also modified the physiological indices of transgenic alfalfa under bicarbonate alkaline stress. In addition, the expression levels of several stress responsive genes were also augmented by GsbZIP67 overexpression. CONCLUSIONS: Collectively, in this study, we demonstrated that GsbZIP67 acted as a positive regulator of plant tolerance to bicarbonate alkaline stress. These results provide direct genetic evidence of group S2 bZIPs in bicarbonate alkaline stress, and will facilitate further studies concerning the cis-elements and/or downstream genes targeted by GsbZIP67 in stress responses.
XieM, ChungCYL, LiMW, WongFL, WangX, LiuAL, WangZL, LeungAKY, WongTH, TongSW, XiaoZX, FanKJ, NgMS, QiXP, YangLF, DengTQ, HeLJ, ChenL, FuAS, DingQ, HeJX, ChungG, IsobeS, TanabataT, ValliyodanB, NguyenHT, CannonSB, FoyerCH, ChanTF, LamHM (2019). A reference-grade wild soybean genome Nat Commun 10, 1216. DOI:10.1038/s41467-019-09142-9URLPMID:30872580 [本文引用: 1] Efficient crop improvement depends on the application of accurate genetic information contained in diverse germplasm resources. Here we report a reference-grade genome of wild soybean accession W05, with a final assembled genome size of 1013.2 Mb and a contig N50 of 3.3 Mb. The analytical power of the W05 genome is demonstrated by several examples. First, we identify an inversion at the locus determining seed coat color during domestication. Second, a translocation event between chromosomes 11 and 13 of some genotypes is shown to interfere with the assignment of QTLs. Third, we find a region containing copy number variations of the Kunitz trypsin inhibitor (KTI) genes. Such findings illustrate the power of this assembly in the analysis of large structural variations in soybean germplasm collections. The wild soybean genome assembly has wide applications in comparative genomic and evolutionary studies, as well as in crop breeding and improvement programs.
YangL, JiW, GaoP, LiY, CaiH, BaiX, ChenQ, ZhuYM (2012). GsAPK, an ABA-activated and calcium-independent SnRK2-type kinase from G. soja, mediates the regulation of plant tolerance to salinity and ABA stress PLoS One 3, e33838. [本文引用: 2]
YangL, JiW, ZhuYM, GaoP, LiY, CaiH, BaiX, GuoDJ (2010). GsCBRLK, a calcium/calmodulin-binding receptor-like kinase, is a positive regulator of plant tolerance to salt and ABA stress J Exp Bot 61, 2519-2533. DOI:10.1093/jxb/erq084URLPMID:20400529 [本文引用: 2] Calcium/calmodulin-dependent kinases play vital roles in protein phosphorylation in eukaryotes, yet little is known about the phosphorylation process of calcium/calmodulin-dependent protein kinase and its role in stress signal transduction in plants. A novel plant-specific calcium-dependent calmodulin-binding receptor-like kinase (GsCBRLK) has been isolated from Glycine soja. A subcellular localization study using GFP fusion protein indicated that GsCBRLK is localized in the plasma membrane. Binding assays demonstrated that calmodulin binds to GsCBRLK with an affinity of 25.9 nM in a calcium-dependent manner and the binding motif lies between amino acids 147 to169 within subdomain II of the kinase domain. GsCBRLK undergoes autophosphorylation and Myelin Basis Protein phosphorylation in the presence of calcium. It was also found that calcium/calmodulin positively regulates GsCBRLK kinase activity through direct interaction between the calmodulin-binding domain and calmodulin. So, it is likely that GsCBRLK responds to an environmental stimulus in two ways: by increasing the protein expression level and by regulating its kinase activity through the calcium/calmodulin complex. Furthermore, cold, salinity, drought, and ABA stress induce GsCBRLK gene transcripts. Over-expression of GsCBRLK in transgenic Arabidopsis resulted in enhanced plant tolerance to high salinity and ABA and increased the expression pattern of a number of stress gene markers in response to ABA and high salt. These results identify GsCBRLK as a molecular link between the stress- and ABA-induced calcium/calmodulin signal and gene expression in plant cells.
YangL, WuKC, GaoP, LiuXJ, LiGP, WuZJ (2014). GsLRPK, a novel cold-activated leucine-rich repeat receptor-like protein kinase from Glycine soja, is a positive regulator to cold stress tolerance Plant Sci 215-216, 19-28. DOI:10.1016/j.plantsci.2013.10.009URLPMID:24388511 [本文引用: 1] Plant LRR-RLKs serve as protein interaction platforms, and as regulatory modules of protein activation. Here, we report the isolation of a novel plant-specific LRR-RLK from Glycine soja (termed GsLRPK) by differential screening. GsLRPK expression was cold-inducible and shows Ser/Thr protein kinase activity. Subcellular localization studies using GFP fusion protein indicated that GsLRPK is localized in the plasma membrane. Real-time PCR analysis indicated that temperature, salt, drought, and ABA treatment can alter GsLRPK gene transcription in G. soja. However, just protein induced by cold stress not by salinity and ABA treatment in tobacco was found to possess kinase activity. Furthermore, we found that overexpression of GsLRPK in yeast and Arabidopsis can enhance resistance to cold stress and increase the expression of a number of cold responsive gene markers.
YuY, DuanXB, DingXD, ChenC, ZhuD, YinKD, CaoL, SongXW, ZhuPH, LiQ, NisaZU, YuJY, DuJY, SongY, LiHQ, LiuBD, ZhuYM (2017). A novel AP2/ERF family transcription factor from Glycine soja, GsERF71, is a DNA binding protein that positively regulates alkaline stress tolerance in Arabidopsis Plant Mol Biol 94, 509-530. DOI:10.1007/s11103-017-0623-7URLPMID:28681139 [本文引用: 2] KEY MESSAGE: Here we first found that GsERF71, an ERF factor from wild soybean could increase plant alkaline stress tolerance by up-regulating H+-ATPase and by modifing the accumulation of Auxin. Alkaline soils are widely distributed all over the world and greatly limit plant growth and development. In our previous transcriptome analyses, we have identified several ERF (ethylene-responsive factor) genes that responded strongly to bicarbonate stress in the roots of wild soybean G07256 (Glycine soja). In this study, we cloned and functionally characterized one of the genes, GsERF71. When expressed in epidermal cells of onion, GsERF71 localized to the nucleus. It can activate the reporters in yeast cells, and the C-terminus of 170 amino acids is essential for its transactivation activity. Yeast one-hybrid and EMSA assays indicated that GsERF71 specifically binds to the cis-acting elements of the GCC-box, suggesting that GsERF71 may participate in the regulation of transcription of the relevant biotic and abiotic stress-related genes. Furthermore, transgenic Arabidopsis plants overexpressing GsERF71 showed significantly higher tolerance to bicarbonate stress generated by NaHCO3 or KHCO3 than the wild type (WT) plants, i.e., the transgenic plants had greener leaves, longer roots, higher total chlorophyll contents and lower MDA contents. qRT-PCR and rhizosphere acidification assays indicated that the expression level and activity of H(+)-ATPase (AHA2) were enhanced in the transgenic plants under alkaline stress. Further analysis indicated that the expression of auxin biosynthetic genes and IAA contents were altered to a lower extent in the roots of transgenic plants than WT plants under alkaline stress in a short-term. Together, our data suggest that GsERF71 enhances the tolerance to alkaline stress by up-regulating the expression levels of H(+)-ATPase and by modifying auxin accumulation in transgenic plants.
YuY, LiuAL, DuanXB, WangST, SunXL, DuanmuHZ, ZhuD, ChenC, CaoL, XiaoJL, LiQ, NisaZU, ZhuYM, DingXD (2016). GsERF6, an ethylene-responsive factor from Glycine soja, mediates the regulation of plant bicarbonate tolerance in Arabidopsis Planta 244, 681-698. [本文引用: 2]
ZengQY, YangCY, MaQB, LiXP, DongWW, NianH (2012). Identification of wild soybean miRNAs and their target genes responsive to aluminum stress BMC Plant Biol 12, 182. DOI:10.1186/1471-2229-12-182URLPMID:23040172 [本文引用: 1] BACKGROUND: MicroRNAs (miRNAs) play important regulatory roles in development and stress response in plants. Wild soybean (Glycine soja) has undergone long-term natural selection and may have evolved special mechanisms to survive stress conditions as a result. However, little information about miRNAs especially miRNAs responsive to aluminum (Al) stress is available in wild soybean. RESULTS: Two small RNA libraries and two degradome libraries were constructed from the roots of Al-treated and Al-free G. soja seedlings. For miRNA identification, a total of 7,287,655 and 7,035,914 clean reads in Al-treated and Al-free small RNAs libraries, respectively, were generated, and 97 known miRNAs and 31 novel miRNAs were identified. In addition, 49 p3 or p5 strands of known miRNAs were found. Among all the identified miRNAs, the expressions of 30 miRNAs were responsive to Al stress. Through degradome sequencing, 86 genes were identified as targets of the known miRNAs and five genes were found to be the targets of the novel miRNAs obtained in this study. Gene ontology (GO) annotations of target transcripts indicated that 52 target genes cleaved by conserved miRNA families might play roles in the regulation of transcription. Additionally, some genes, such as those for the auxin response factor (ARF), domain-containing disease resistance protein (NB-ARC), leucine-rich repeat and toll/interleukin-1 receptor-like protein (LRR-TIR) domain protein, cation transporting ATPase, Myb transcription factors, and the no apical meristem (NAM) protein, that are known to be responsive to stress, were found to be cleaved under Al stress conditions. CONCLUSIONS: A number of miRNAs and their targets were detected in wild soybean. Some of them that were responsive to biotic and abiotic stresses were regulated by Al stress. These findings provide valuable information to understand the function of miRNAs in Al tolerance.
ZhangJL, WangJX, JiangW, LiuJG, YangSN, GaiJY, LiY (2016). Identification and analysis of NaHCO3 stress responsive genes in wild soybean (Glycine soja) roots by RNA-seq Front Plant Sci 7, 1842. URLPMID:28018382 [本文引用: 1]
ZhouFL, GuoY, QiuLJ (2016). Genome-wide identification and evolutionary analysis of leucine-rich repeat receptor-like protein kinase genes in soybean BMC Plant Biol 16, 58. DOI:10.1186/s12870-016-0744-1URLPMID:26935840 [本文引用: 1] BACKGROUND: Leucine-rich repeat receptor-like kinases (LRR-RLKs) constitute the largest subfamily of receptor-like kinases in plant. A number of reports have demonstrated that plant LRR-RLKs play important roles in growth, development, differentiation, and stress responses. However, no comprehensive analysis of this gene family has been carried out in legume species. RESULTS: Based on the principles of sequence similarity and domain conservation, a total of 467 LRR-RLK genes were identified in soybean genome. The GmLRR-RLKs are non-randomly distributed across all 20 chromosomes of soybean and about 73.3 % of them are located in segmental duplicated regions. The analysis of synonymous substitutions for putative paralogous gene pairs indicated that most of these gene pairs resulted from segmental duplications in soybean genome. Furthermore, the exon/intron organization, motif composition and arrangements were considerably conserved among members of the same groups or subgroups in the constructed phylogenetic tree. The close phylogenetic relationship between soybean LRR-RLK genes with identified Arabidopsis genes in the same group also provided insight into their putative functions. Expression profiling analysis of GmLRR-RLKs suggested that they appeared to be differentially expressed among different tissues and some of duplicated genes exhibited divergent expression patterns. In addition, artificial selected GmLRR-RLKs were also identified by comparing the SNPs between wild and cultivated soybeans and 17 genes were detected in regions previously reported to contain domestication-related QTLs. CONCLUSIONS: Comprehensive and evolutionary analysis of soybean LRR-RLK gene family was performed at whole genome level. The data provides valuable tools in future efforts to identify functional divergence of this gene family and gene diversity among different genotypes in legume species.
ZhuD, BaiX, ChenC, ChenQ, CaiH, LiY, JiW, ZhaiH, LvDK, LuoX, ZhuYM (2011). GsTIFY10, a novel positive regulator of plant tolerance to bicarbonate stress and a repressor of jasmonate signaling Plant Mol Biol 77, 285-297. [本文引用: 3]
ZhuD, BaiX, LuoX, ChenQ, CaiH, JiW, ZhuYM (2013). Identification of wild soybean (Glycine soja) TIFY family genes and their expression profiling analysis under bicarbonate stress Plant Cell Rep 32, 263-272. DOI:10.1007/s00299-012-1360-7URLPMID:23090726 [本文引用: 1] Wild soybean (Glycine soja L. G07256) exhibits a greater adaptability to soil bicarbonate stress than cultivated soybean, and recent discoveries show that TIFY family genes are involved in the response to several abiotic stresses. A genomic and transcriptomic analysis of all TIFY genes in G. soja, compared with G. max, will provide insight into the function of this gene family in plant bicarbonate stress response. This article identified and characterized 34 TIFY genes in G. soja. Sequence analyses indicated that most GsTIFY proteins had two conserved domains: TIFY and Jas. Phylogenetic analyses suggested that these GsTIFY genes could be classified into two groups. A clustering analysis of all GsTIFY transcript expression profiles from bicarbonate stress treated G. soja showed that there were five different transcript patterns in leaves and six different transcript patterns in roots when the GsTIFY family responds to bicarbonate stress. Moreover, the expression level changes of all TIFY genes in cultivated soybean, treated with bicarbonate stress, were also verified. The expression comparison analysis of TIFYs between wild and cultivated soybeans confirmed that, different from the cultivated soybean, GsTIFY (10a, 10b, 10c, 10d, 10e, 10f, 11a, and 11b) were dramatically up-regulated at the early stage of stress, while GsTIFY 1c and 2b were significantly up-regulated at the later period of stress. The frequently stress responsive and diverse expression profiles of the GsTIFY gene family suggests that this family may play important roles in plant environmental stress responses and adaptation.
ZhuD, CaiH, LuoX, BaiX, DeyholosMK, ChenQ, ChenC, JiW, ZhuYM (2012). Over-expression of a novel JAZ family gene from Glycine soja, increases salt and alkali stress tolerance Biochem Biophys Res Commun 426, 273-279. DOI:10.1016/j.bbrc.2012.08.086URLPMID:22943855 [本文引用: 4] Salt and alkali stress are two of the main environmental factors limiting crop production. Recent discoveries show that the JAZ family encodes plant-specific genes involved in jasmonate signaling. However, there is only limited information about this gene family in abiotic stress response, and in wild soybean (Glycine soja), which is a species noted for its tolerance to alkali and salinity. Here, we isolated and characterized a novel JAZ family gene, GsJAZ2, from G. soja. Transcript abundance of GsJAZ2 increased following exposure to salt, alkali, cold and drought. Over-expression of GsJAZ2 in Arabidopsis resulted in enhanced plant tolerance to salt and alkali stress. The expression levels of some alkali stress response and stress-inducible marker genes were significantly higher in the GsJAZ2 overexpression lines as compared to wild-type plants. Subcellular localization studies using a GFP fusion protein showed that GsJAZ2 was localized to the nucleus. These results suggest that the newly isolated wild soybean GsJAZ2 is a positive regulator of plant salt and alkali stress tolerance.
ZhuD, LiRT, LiuX, SunMZ, WuJ, ZhangN, ZhuYM (2014). The positive regulatory roles of the TIFY10 proteins in plant responses to alkaline stress PLoS One 9, e111984. URLPMID:25375909 [本文引用: 3]
ZulawskiM, SchulzeG, BraginetsR, HartmannS, SchulzeWX (2014). The Arabidopsis kinome: phylogeny and evolutionary insights into functional diversification BMC Genomics 15, 548. DOI:10.1186/1471-2164-15-548URL [本文引用: 1]
野生大豆GsbZIP33基因的分离及胁迫耐性分析 1 2011
... Transcription factor involved in stress tolerance of wild soybean Table 2
编号
基因
编码蛋白
胁迫类型
参考文献
1
GsZFP1
ZFP转录因子
冷、干旱、盐和ABA胁迫
罗晓等, 2012; Luo et al., 2012a, 2012b; Tang et al., 2013
... 已报道的野生大豆转录组测序研究均表明转录因子在野生大豆耐逆过程中发挥重要作用(表2).GsZFP1编码1个缺少N端QALGGH结构域的C2H2型锌指蛋白, 受冷、干旱、ABA和盐诱导表达(罗晓等, 2012; Luo et al., 2012a); 并可通过CBF依赖和CBF不依赖途径提高转基因拟南芥的耐冷性(Luo et al., 2012a); 还通过调控气孔关闭减少水分散失增强转基因拟南芥和苜蓿的抗旱性(Luo et al., 2012a, 2012b; Tang et al., 2013).GsZFP1超表达增强转基因苜蓿的耐盐性(Tang et al., 2013).因此, GsZFP1作为野生大豆耐逆应答信号通路中的关键基因, 需进一步解析其在野生大豆耐逆应答中的分子机制. ...
... Transcription factor involved in stress tolerance of wild soybean Table 2
编号
基因
编码蛋白
胁迫类型
参考文献
1
GsZFP1
ZFP转录因子
冷、干旱、盐和ABA胁迫
罗晓等, 2012; Luo et al., 2012a, 2012b; Tang et al., 2013
A novel Glycine soja homeodomain-leucine zipper (HD-Zip) I gene, Gshdz4, positively regulates bicarbonate tolerance and responds to osmotic stress in Arabidopsis 1 2016
... Transcription factor involved in stress tolerance of wild soybean Table 2
编号
基因
编码蛋白
胁迫类型
参考文献
1
GsZFP1
ZFP转录因子
冷、干旱、盐和ABA胁迫
罗晓等, 2012; Luo et al., 2012a, 2012b; Tang et al., 2013
Over-expression of a glutathione S-transferase gene, GsGST, from wild soybean (Glycine soja) enhances drought and salt tolerance in transgenic tobacco 2 2010
Transcriptomic analysis of Glycine soja and G. max seedlings and functional characterization of GsGSTU24 and GsGSTU42 genes under submergence stress 2 2020
GsSKP21, a Glycine soja S-phase kinase-associated protein, mediates the regulation of plant alkaline tolerance and ABA sensitivity 0 2015
Soybean kinome: functional classification and gene expression patterns 2 2015
... 蛋白激酶通过磷酸化下游靶蛋白, 启动或关闭信号转导通路, 调控植物逆境应答.大豆中4.67%的基因编码蛋白激酶(protein kinase), 其中约65%属于类受体蛋白激酶(receptor like kinases, RLKs) (Zulawski et al., 2014; Liu et al., 2015b).RLKs蛋白一般包含1个胞外结构域、1个跨膜结构域和1个胞内激酶结构域. ...
... 研究人员还发现了其它参与逆境应答的野生大豆蛋白激酶基因(表1), 如GsAPK (Yang et al., 2012)、GsPPCK1 (魏正巍等, 2013)和GsPPCK3 (Sun et al., 2014a).需要指出的是, 大豆4.67% (约2 166个)基因编码蛋白激酶(Liu et al., 2015b).虽然已报道了多个调控野生大豆耐逆性的蛋白激酶, 但对蛋白激酶调控的耐逆性分子机制知之甚少, 尚待进一步探索. ...
GsZFP1, a new Cys2/His2-type zinc-finger protein, is a positive regulator of plant tolerance to cold and drought stress 4 2012
... 已报道的野生大豆转录组测序研究均表明转录因子在野生大豆耐逆过程中发挥重要作用(表2).GsZFP1编码1个缺少N端QALGGH结构域的C2H2型锌指蛋白, 受冷、干旱、ABA和盐诱导表达(罗晓等, 2012; Luo et al., 2012a); 并可通过CBF依赖和CBF不依赖途径提高转基因拟南芥的耐冷性(Luo et al., 2012a); 还通过调控气孔关闭减少水分散失增强转基因拟南芥和苜蓿的抗旱性(Luo et al., 2012a, 2012b; Tang et al., 2013).GsZFP1超表达增强转基因苜蓿的耐盐性(Tang et al., 2013).因此, GsZFP1作为野生大豆耐逆应答信号通路中的关键基因, 需进一步解析其在野生大豆耐逆应答中的分子机制. ...
... ); 并可通过CBF依赖和CBF不依赖途径提高转基因拟南芥的耐冷性(Luo et al., 2012a); 还通过调控气孔关闭减少水分散失增强转基因拟南芥和苜蓿的抗旱性(Luo et al., 2012a, 2012b; Tang et al., 2013).GsZFP1超表达增强转基因苜蓿的耐盐性(Tang et al., 2013).因此, GsZFP1作为野生大豆耐逆应答信号通路中的关键基因, 需进一步解析其在野生大豆耐逆应答中的分子机制. ...
... ); 还通过调控气孔关闭减少水分散失增强转基因拟南芥和苜蓿的抗旱性(Luo et al., 2012a, 2012b; Tang et al., 2013).GsZFP1超表达增强转基因苜蓿的耐盐性(Tang et al., 2013).因此, GsZFP1作为野生大豆耐逆应答信号通路中的关键基因, 需进一步解析其在野生大豆耐逆应答中的分子机制. ...
... Transcription factor involved in stress tolerance of wild soybean Table 2
编号
基因
编码蛋白
胁迫类型
参考文献
1
GsZFP1
ZFP转录因子
冷、干旱、盐和ABA胁迫
罗晓等, 2012; Luo et al., 2012a, 2012b; Tang et al., 2013
Over-expression of GsZFP1, an ABA-responsive C2H2-type zinc finger protein lacking a QALGGH motif, reduces ABA sensitivity and decreases stomata size 2 2012
... 已报道的野生大豆转录组测序研究均表明转录因子在野生大豆耐逆过程中发挥重要作用(表2).GsZFP1编码1个缺少N端QALGGH结构域的C2H2型锌指蛋白, 受冷、干旱、ABA和盐诱导表达(罗晓等, 2012; Luo et al., 2012a); 并可通过CBF依赖和CBF不依赖途径提高转基因拟南芥的耐冷性(Luo et al., 2012a); 还通过调控气孔关闭减少水分散失增强转基因拟南芥和苜蓿的抗旱性(Luo et al., 2012a, 2012b; Tang et al., 2013).GsZFP1超表达增强转基因苜蓿的耐盐性(Tang et al., 2013).因此, GsZFP1作为野生大豆耐逆应答信号通路中的关键基因, 需进一步解析其在野生大豆耐逆应答中的分子机制. ...
... Transcription factor involved in stress tolerance of wild soybean Table 2
编号
基因
编码蛋白
胁迫类型
参考文献
1
GsZFP1
ZFP转录因子
冷、干旱、盐和ABA胁迫
罗晓等, 2012; Luo et al., 2012a, 2012b; Tang et al., 2013
Overexpression of the wild soybean R2R3-MYB transcription factor GsMYB15 enhances resistance to salt stress and Helicoverpa armigera in transgenic Arabidopsis 1 2018
... Transcription factor involved in stress tolerance of wild soybean Table 2
编号
基因
编码蛋白
胁迫类型
参考文献
1
GsZFP1
ZFP转录因子
冷、干旱、盐和ABA胁迫
罗晓等, 2012; Luo et al., 2012a, 2012b; Tang et al., 2013
Ectopic expression of GsSRK in Medicago sativa reveals its involvement in plant architecture and salt stress responses 1 2018
... Protein kinases implicated in stress tolerance of wild soybean Table 1
编号
基因
编码蛋白
胁迫类型
参考文献
1
GsLRPK
LRR类受体蛋白激酶
冷、干旱、盐和ABA胁迫
杨靓等, 2012; Yang et al., 2014
2
GsRLCK
胞浆类受体蛋白激酶
ABA、盐、碱和干旱胁迫
Sun et al., 2013a
3
GsCBRLK
Ca2+/CaM结合类受体蛋白激酶
冷、盐、干旱和ABA胁迫
Yang et al., 2010; Bai et al., 2013; 赵阳等, 2014
4
GsSRK
G型凝集素类受体蛋白激酶
ABA、盐和干旱胁迫
Sun et al., 2013b, 2018
5
GsAPK
不依赖Ca2+的丝/苏氨酸类蛋白 激酶
冷、盐、干旱和ABA胁迫
Yang et al., 2012
6
GsPPCK1
磷酸烯醇式丙酮酸羧化酶激酶
碱胁迫
魏正巍等, 2013
7
GsPPCK3
磷酸烯醇式丙酮酸羧化酶激酶
碱胁迫
Sun et al., 2014a
胞质类受体蛋白激酶RLCKs (receptor-like cytoplasmic kinases)是一类特殊的RLKs, 其缺少其它RLKs具有的胞外结构域(表1).Sun等(2013a)分离了1个受逆境胁迫诱导的野生大豆RLCK基因GsRLCK, 研究发现该基因在拟南芥中过表达可降低对ABA的敏感性, 提高耐盐性和耐旱性.Yang等(2010)从野生大豆中分离了1个Ca2+/CaM结合的RLK基因GsCBRLK, 其表达受冷、盐、干旱和ABA诱导.GsCBRLK过表达可显著提高转基因拟南芥、苜蓿(Medicago sativa)和大豆对ABA、高盐和碱胁迫的耐受性(Bai et al., 2013; 赵阳等, 2014; Ji et al., 2016b).后续, Sun等 (2014b, 2016b, 2019, 2021)进一步鉴定获得了4个GsCBRLK互作蛋白.其中, GsBET11a编码1个SNARE转运蛋白, 通过C端跨膜结构域与GsCBRLK互作, GsBET11a过表达可提高转基因拟南芥和大豆的耐盐性(Sun et al., 2021).GsCBRLK通过N端可变结构域与GsMSRB5a (methionine sulfoxide reductase B5a)互作, 并通过调控ROS稳态参与盐碱胁迫应答(Sun et al., 2016b).此外, GsCBRLK的N端可变域也能与GsPM30及多个Group 3 LEA (late-embryogenesis abundant protein)蛋白互作, GsPM30在拟南芥中过表达会增强幼苗期和成苗期对高盐和脱水的耐性(Sun et al., 2019).GsCPI14 (Glycine soja cystatin protein 14)编码一个蛋白酶抑制剂, 正调控植物的耐碱性(Sun et al., 2014b).另外, Sun等(2013b)获得1个受ABA、盐和干旱胁迫诱导表达的G型凝集素RLK基因GsSRK.该基因过表达促进转基因拟南芥盐胁迫下的种子萌发、幼苗生长和种子产量, 且当缺失N端信号肽和G型凝集素结构域的截短型GsSRK (GsSRK-t)转入苜蓿, 苜蓿的分枝和盐胁迫下的生物量积累增多. ...
A late embryogenesis abundant protein GsPM30 interacts with a receptor like cytoplasmic kinase GsCBRLK and regulates environmental stress responses 2 2019
... 胞质类受体蛋白激酶RLCKs (receptor-like cytoplasmic kinases)是一类特殊的RLKs, 其缺少其它RLKs具有的胞外结构域(表1).Sun等(2013a)分离了1个受逆境胁迫诱导的野生大豆RLCK基因GsRLCK, 研究发现该基因在拟南芥中过表达可降低对ABA的敏感性, 提高耐盐性和耐旱性.Yang等(2010)从野生大豆中分离了1个Ca2+/CaM结合的RLK基因GsCBRLK, 其表达受冷、盐、干旱和ABA诱导.GsCBRLK过表达可显著提高转基因拟南芥、苜蓿(Medicago sativa)和大豆对ABA、高盐和碱胁迫的耐受性(Bai et al., 2013; 赵阳等, 2014; Ji et al., 2016b).后续, Sun等 (2014b, 2016b, 2019, 2021)进一步鉴定获得了4个GsCBRLK互作蛋白.其中, GsBET11a编码1个SNARE转运蛋白, 通过C端跨膜结构域与GsCBRLK互作, GsBET11a过表达可提高转基因拟南芥和大豆的耐盐性(Sun et al., 2021).GsCBRLK通过N端可变结构域与GsMSRB5a (methionine sulfoxide reductase B5a)互作, 并通过调控ROS稳态参与盐碱胁迫应答(Sun et al., 2016b).此外, GsCBRLK的N端可变域也能与GsPM30及多个Group 3 LEA (late-embryogenesis abundant protein)蛋白互作, GsPM30在拟南芥中过表达会增强幼苗期和成苗期对高盐和脱水的耐性(Sun et al., 2019).GsCPI14 (Glycine soja cystatin protein 14)编码一个蛋白酶抑制剂, 正调控植物的耐碱性(Sun et al., 2014b).另外, Sun等(2013b)获得1个受ABA、盐和干旱胁迫诱导表达的G型凝集素RLK基因GsSRK.该基因过表达促进转基因拟南芥盐胁迫下的种子萌发、幼苗生长和种子产量, 且当缺失N端信号肽和G型凝集素结构域的截短型GsSRK (GsSRK-t)转入苜蓿, 苜蓿的分枝和盐胁迫下的生物量积累增多. ...
... 在拟南芥中过表达会增强幼苗期和成苗期对高盐和脱水的耐性(Sun et al., 2019).GsCPI14 (Glycine soja cystatin protein 14)编码一个蛋白酶抑制剂, 正调控植物的耐碱性(Sun et al., 2014b).另外, Sun等(2013b)获得1个受ABA、盐和干旱胁迫诱导表达的G型凝集素RLK基因GsSRK.该基因过表达促进转基因拟南芥盐胁迫下的种子萌发、幼苗生长和种子产量, 且当缺失N端信号肽和G型凝集素结构域的截短型GsSRK (GsSRK-t)转入苜蓿, 苜蓿的分枝和盐胁迫下的生物量积累增多. ...
Ectopic expression of GsPPCK3 and SCMRP in Medicago sativa enhances plant alkaline stress tolerance and methionine content 2 2014
... Protein kinases implicated in stress tolerance of wild soybean Table 1
编号
基因
编码蛋白
胁迫类型
参考文献
1
GsLRPK
LRR类受体蛋白激酶
冷、干旱、盐和ABA胁迫
杨靓等, 2012; Yang et al., 2014
2
GsRLCK
胞浆类受体蛋白激酶
ABA、盐、碱和干旱胁迫
Sun et al., 2013a
3
GsCBRLK
Ca2+/CaM结合类受体蛋白激酶
冷、盐、干旱和ABA胁迫
Yang et al., 2010; Bai et al., 2013; 赵阳等, 2014
4
GsSRK
G型凝集素类受体蛋白激酶
ABA、盐和干旱胁迫
Sun et al., 2013b, 2018
5
GsAPK
不依赖Ca2+的丝/苏氨酸类蛋白 激酶
冷、盐、干旱和ABA胁迫
Yang et al., 2012
6
GsPPCK1
磷酸烯醇式丙酮酸羧化酶激酶
碱胁迫
魏正巍等, 2013
7
GsPPCK3
磷酸烯醇式丙酮酸羧化酶激酶
碱胁迫
Sun et al., 2014a
胞质类受体蛋白激酶RLCKs (receptor-like cytoplasmic kinases)是一类特殊的RLKs, 其缺少其它RLKs具有的胞外结构域(表1).Sun等(2013a)分离了1个受逆境胁迫诱导的野生大豆RLCK基因GsRLCK, 研究发现该基因在拟南芥中过表达可降低对ABA的敏感性, 提高耐盐性和耐旱性.Yang等(2010)从野生大豆中分离了1个Ca2+/CaM结合的RLK基因GsCBRLK, 其表达受冷、盐、干旱和ABA诱导.GsCBRLK过表达可显著提高转基因拟南芥、苜蓿(Medicago sativa)和大豆对ABA、高盐和碱胁迫的耐受性(Bai et al., 2013; 赵阳等, 2014; Ji et al., 2016b).后续, Sun等 (2014b, 2016b, 2019, 2021)进一步鉴定获得了4个GsCBRLK互作蛋白.其中, GsBET11a编码1个SNARE转运蛋白, 通过C端跨膜结构域与GsCBRLK互作, GsBET11a过表达可提高转基因拟南芥和大豆的耐盐性(Sun et al., 2021).GsCBRLK通过N端可变结构域与GsMSRB5a (methionine sulfoxide reductase B5a)互作, 并通过调控ROS稳态参与盐碱胁迫应答(Sun et al., 2016b).此外, GsCBRLK的N端可变域也能与GsPM30及多个Group 3 LEA (late-embryogenesis abundant protein)蛋白互作, GsPM30在拟南芥中过表达会增强幼苗期和成苗期对高盐和脱水的耐性(Sun et al., 2019).GsCPI14 (Glycine soja cystatin protein 14)编码一个蛋白酶抑制剂, 正调控植物的耐碱性(Sun et al., 2014b).另外, Sun等(2013b)获得1个受ABA、盐和干旱胁迫诱导表达的G型凝集素RLK基因GsSRK.该基因过表达促进转基因拟南芥盐胁迫下的种子萌发、幼苗生长和种子产量, 且当缺失N端信号肽和G型凝集素结构域的截短型GsSRK (GsSRK-t)转入苜蓿, 苜蓿的分枝和盐胁迫下的生物量积累增多. ...
... 研究人员还发现了其它参与逆境应答的野生大豆蛋白激酶基因(表1), 如GsAPK (Yang et al., 2012)、GsPPCK1 (魏正巍等, 2013)和GsPPCK3 (Sun et al., 2014a).需要指出的是, 大豆4.67% (约2 166个)基因编码蛋白激酶(Liu et al., 2015b).虽然已报道了多个调控野生大豆耐逆性的蛋白激酶, 但对蛋白激酶调控的耐逆性分子机制知之甚少, 尚待进一步探索. ...
Wild soybean SNARE proteins BET1s mediate the subcellular localization of the cytoplasmic receptor-like kinases CRCK1s to modulate salt stress responses 2 2021
... 胞质类受体蛋白激酶RLCKs (receptor-like cytoplasmic kinases)是一类特殊的RLKs, 其缺少其它RLKs具有的胞外结构域(表1).Sun等(2013a)分离了1个受逆境胁迫诱导的野生大豆RLCK基因GsRLCK, 研究发现该基因在拟南芥中过表达可降低对ABA的敏感性, 提高耐盐性和耐旱性.Yang等(2010)从野生大豆中分离了1个Ca2+/CaM结合的RLK基因GsCBRLK, 其表达受冷、盐、干旱和ABA诱导.GsCBRLK过表达可显著提高转基因拟南芥、苜蓿(Medicago sativa)和大豆对ABA、高盐和碱胁迫的耐受性(Bai et al., 2013; 赵阳等, 2014; Ji et al., 2016b).后续, Sun等 (2014b, 2016b, 2019, 2021)进一步鉴定获得了4个GsCBRLK互作蛋白.其中, GsBET11a编码1个SNARE转运蛋白, 通过C端跨膜结构域与GsCBRLK互作, GsBET11a过表达可提高转基因拟南芥和大豆的耐盐性(Sun et al., 2021).GsCBRLK通过N端可变结构域与GsMSRB5a (methionine sulfoxide reductase B5a)互作, 并通过调控ROS稳态参与盐碱胁迫应答(Sun et al., 2016b).此外, GsCBRLK的N端可变域也能与GsPM30及多个Group 3 LEA (late-embryogenesis abundant protein)蛋白互作, GsPM30在拟南芥中过表达会增强幼苗期和成苗期对高盐和脱水的耐性(Sun et al., 2019).GsCPI14 (Glycine soja cystatin protein 14)编码一个蛋白酶抑制剂, 正调控植物的耐碱性(Sun et al., 2014b).另外, Sun等(2013b)获得1个受ABA、盐和干旱胁迫诱导表达的G型凝集素RLK基因GsSRK.该基因过表达促进转基因拟南芥盐胁迫下的种子萌发、幼苗生长和种子产量, 且当缺失N端信号肽和G型凝集素结构域的截短型GsSRK (GsSRK-t)转入苜蓿, 苜蓿的分枝和盐胁迫下的生物量积累增多. ...
... 过表达可提高转基因拟南芥和大豆的耐盐性(Sun et al., 2021).GsCBRLK通过N端可变结构域与GsMSRB5a (methionine sulfoxide reductase B5a)互作, 并通过调控ROS稳态参与盐碱胁迫应答(Sun et al., 2016b).此外, GsCBRLK的N端可变域也能与GsPM30及多个Group 3 LEA (late-embryogenesis abundant protein)蛋白互作, GsPM30在拟南芥中过表达会增强幼苗期和成苗期对高盐和脱水的耐性(Sun et al., 2019).GsCPI14 (Glycine soja cystatin protein 14)编码一个蛋白酶抑制剂, 正调控植物的耐碱性(Sun et al., 2014b).另外, Sun等(2013b)获得1个受ABA、盐和干旱胁迫诱导表达的G型凝集素RLK基因GsSRK.该基因过表达促进转基因拟南芥盐胁迫下的种子萌发、幼苗生长和种子产量, 且当缺失N端信号肽和G型凝集素结构域的截短型GsSRK (GsSRK-t)转入苜蓿, 苜蓿的分枝和盐胁迫下的生物量积累增多. ...
A Glycine soja methionine sulfoxide reductase B5a interacts with the Ca2+/CAM-binding kinase GsCBRLK and activates ROS signaling under carbonate alkaline stress 2 2016
... 胞质类受体蛋白激酶RLCKs (receptor-like cytoplasmic kinases)是一类特殊的RLKs, 其缺少其它RLKs具有的胞外结构域(表1).Sun等(2013a)分离了1个受逆境胁迫诱导的野生大豆RLCK基因GsRLCK, 研究发现该基因在拟南芥中过表达可降低对ABA的敏感性, 提高耐盐性和耐旱性.Yang等(2010)从野生大豆中分离了1个Ca2+/CaM结合的RLK基因GsCBRLK, 其表达受冷、盐、干旱和ABA诱导.GsCBRLK过表达可显著提高转基因拟南芥、苜蓿(Medicago sativa)和大豆对ABA、高盐和碱胁迫的耐受性(Bai et al., 2013; 赵阳等, 2014; Ji et al., 2016b).后续, Sun等 (2014b, 2016b, 2019, 2021)进一步鉴定获得了4个GsCBRLK互作蛋白.其中, GsBET11a编码1个SNARE转运蛋白, 通过C端跨膜结构域与GsCBRLK互作, GsBET11a过表达可提高转基因拟南芥和大豆的耐盐性(Sun et al., 2021).GsCBRLK通过N端可变结构域与GsMSRB5a (methionine sulfoxide reductase B5a)互作, 并通过调控ROS稳态参与盐碱胁迫应答(Sun et al., 2016b).此外, GsCBRLK的N端可变域也能与GsPM30及多个Group 3 LEA (late-embryogenesis abundant protein)蛋白互作, GsPM30在拟南芥中过表达会增强幼苗期和成苗期对高盐和脱水的耐性(Sun et al., 2019).GsCPI14 (Glycine soja cystatin protein 14)编码一个蛋白酶抑制剂, 正调控植物的耐碱性(Sun et al., 2014b).另外, Sun等(2013b)获得1个受ABA、盐和干旱胁迫诱导表达的G型凝集素RLK基因GsSRK.该基因过表达促进转基因拟南芥盐胁迫下的种子萌发、幼苗生长和种子产量, 且当缺失N端信号肽和G型凝集素结构域的截短型GsSRK (GsSRK-t)转入苜蓿, 苜蓿的分枝和盐胁迫下的生物量积累增多. ...
... ).GsCBRLK通过N端可变结构域与GsMSRB5a (methionine sulfoxide reductase B5a)互作, 并通过调控ROS稳态参与盐碱胁迫应答(Sun et al., 2016b).此外, GsCBRLK的N端可变域也能与GsPM30及多个Group 3 LEA (late-embryogenesis abundant protein)蛋白互作, GsPM30在拟南芥中过表达会增强幼苗期和成苗期对高盐和脱水的耐性(Sun et al., 2019).GsCPI14 (Glycine soja cystatin protein 14)编码一个蛋白酶抑制剂, 正调控植物的耐碱性(Sun et al., 2014b).另外, Sun等(2013b)获得1个受ABA、盐和干旱胁迫诱导表达的G型凝集素RLK基因GsSRK.该基因过表达促进转基因拟南芥盐胁迫下的种子萌发、幼苗生长和种子产量, 且当缺失N端信号肽和G型凝集素结构域的截短型GsSRK (GsSRK-t)转入苜蓿, 苜蓿的分枝和盐胁迫下的生物量积累增多. ...
A Glycine soja ABA-responsive receptor-like cytoplasmic kinase, GsRLCK, positively controls plant tolerance to salt and drought stresses 2 2013
... Protein kinases implicated in stress tolerance of wild soybean Table 1
编号
基因
编码蛋白
胁迫类型
参考文献
1
GsLRPK
LRR类受体蛋白激酶
冷、干旱、盐和ABA胁迫
杨靓等, 2012; Yang et al., 2014
2
GsRLCK
胞浆类受体蛋白激酶
ABA、盐、碱和干旱胁迫
Sun et al., 2013a
3
GsCBRLK
Ca2+/CaM结合类受体蛋白激酶
冷、盐、干旱和ABA胁迫
Yang et al., 2010; Bai et al., 2013; 赵阳等, 2014
4
GsSRK
G型凝集素类受体蛋白激酶
ABA、盐和干旱胁迫
Sun et al., 2013b, 2018
5
GsAPK
不依赖Ca2+的丝/苏氨酸类蛋白 激酶
冷、盐、干旱和ABA胁迫
Yang et al., 2012
6
GsPPCK1
磷酸烯醇式丙酮酸羧化酶激酶
碱胁迫
魏正巍等, 2013
7
GsPPCK3
磷酸烯醇式丙酮酸羧化酶激酶
碱胁迫
Sun et al., 2014a
胞质类受体蛋白激酶RLCKs (receptor-like cytoplasmic kinases)是一类特殊的RLKs, 其缺少其它RLKs具有的胞外结构域(表1).Sun等(2013a)分离了1个受逆境胁迫诱导的野生大豆RLCK基因GsRLCK, 研究发现该基因在拟南芥中过表达可降低对ABA的敏感性, 提高耐盐性和耐旱性.Yang等(2010)从野生大豆中分离了1个Ca2+/CaM结合的RLK基因GsCBRLK, 其表达受冷、盐、干旱和ABA诱导.GsCBRLK过表达可显著提高转基因拟南芥、苜蓿(Medicago sativa)和大豆对ABA、高盐和碱胁迫的耐受性(Bai et al., 2013; 赵阳等, 2014; Ji et al., 2016b).后续, Sun等 (2014b, 2016b, 2019, 2021)进一步鉴定获得了4个GsCBRLK互作蛋白.其中, GsBET11a编码1个SNARE转运蛋白, 通过C端跨膜结构域与GsCBRLK互作, GsBET11a过表达可提高转基因拟南芥和大豆的耐盐性(Sun et al., 2021).GsCBRLK通过N端可变结构域与GsMSRB5a (methionine sulfoxide reductase B5a)互作, 并通过调控ROS稳态参与盐碱胁迫应答(Sun et al., 2016b).此外, GsCBRLK的N端可变域也能与GsPM30及多个Group 3 LEA (late-embryogenesis abundant protein)蛋白互作, GsPM30在拟南芥中过表达会增强幼苗期和成苗期对高盐和脱水的耐性(Sun et al., 2019).GsCPI14 (Glycine soja cystatin protein 14)编码一个蛋白酶抑制剂, 正调控植物的耐碱性(Sun et al., 2014b).另外, Sun等(2013b)获得1个受ABA、盐和干旱胁迫诱导表达的G型凝集素RLK基因GsSRK.该基因过表达促进转基因拟南芥盐胁迫下的种子萌发、幼苗生长和种子产量, 且当缺失N端信号肽和G型凝集素结构域的截短型GsSRK (GsSRK-t)转入苜蓿, 苜蓿的分枝和盐胁迫下的生物量积累增多. ...
... 胞质类受体蛋白激酶RLCKs (receptor-like cytoplasmic kinases)是一类特殊的RLKs, 其缺少其它RLKs具有的胞外结构域(表1).Sun等(2013a)分离了1个受逆境胁迫诱导的野生大豆RLCK基因GsRLCK, 研究发现该基因在拟南芥中过表达可降低对ABA的敏感性, 提高耐盐性和耐旱性.Yang等(2010)从野生大豆中分离了1个Ca2+/CaM结合的RLK基因GsCBRLK, 其表达受冷、盐、干旱和ABA诱导.GsCBRLK过表达可显著提高转基因拟南芥、苜蓿(Medicago sativa)和大豆对ABA、高盐和碱胁迫的耐受性(Bai et al., 2013; 赵阳等, 2014; Ji et al., 2016b).后续, Sun等 (2014b, 2016b, 2019, 2021)进一步鉴定获得了4个GsCBRLK互作蛋白.其中, GsBET11a编码1个SNARE转运蛋白, 通过C端跨膜结构域与GsCBRLK互作, GsBET11a过表达可提高转基因拟南芥和大豆的耐盐性(Sun et al., 2021).GsCBRLK通过N端可变结构域与GsMSRB5a (methionine sulfoxide reductase B5a)互作, 并通过调控ROS稳态参与盐碱胁迫应答(Sun et al., 2016b).此外, GsCBRLK的N端可变域也能与GsPM30及多个Group 3 LEA (late-embryogenesis abundant protein)蛋白互作, GsPM30在拟南芥中过表达会增强幼苗期和成苗期对高盐和脱水的耐性(Sun et al., 2019).GsCPI14 (Glycine soja cystatin protein 14)编码一个蛋白酶抑制剂, 正调控植物的耐碱性(Sun et al., 2014b).另外, Sun等(2013b)获得1个受ABA、盐和干旱胁迫诱导表达的G型凝集素RLK基因GsSRK.该基因过表达促进转基因拟南芥盐胁迫下的种子萌发、幼苗生长和种子产量, 且当缺失N端信号肽和G型凝集素结构域的截短型GsSRK (GsSRK-t)转入苜蓿, 苜蓿的分枝和盐胁迫下的生物量积累增多. ...
A novel Glycine soja cysteine proteinase inhibitor GsCPI14, interacting with the calcium/calmodulin-binding receptor-like kinase GsCBRLK, regulated plant tolerance to alkali stress 2 2014
... 胞质类受体蛋白激酶RLCKs (receptor-like cytoplasmic kinases)是一类特殊的RLKs, 其缺少其它RLKs具有的胞外结构域(表1).Sun等(2013a)分离了1个受逆境胁迫诱导的野生大豆RLCK基因GsRLCK, 研究发现该基因在拟南芥中过表达可降低对ABA的敏感性, 提高耐盐性和耐旱性.Yang等(2010)从野生大豆中分离了1个Ca2+/CaM结合的RLK基因GsCBRLK, 其表达受冷、盐、干旱和ABA诱导.GsCBRLK过表达可显著提高转基因拟南芥、苜蓿(Medicago sativa)和大豆对ABA、高盐和碱胁迫的耐受性(Bai et al., 2013; 赵阳等, 2014; Ji et al., 2016b).后续, Sun等 (2014b, 2016b, 2019, 2021)进一步鉴定获得了4个GsCBRLK互作蛋白.其中, GsBET11a编码1个SNARE转运蛋白, 通过C端跨膜结构域与GsCBRLK互作, GsBET11a过表达可提高转基因拟南芥和大豆的耐盐性(Sun et al., 2021).GsCBRLK通过N端可变结构域与GsMSRB5a (methionine sulfoxide reductase B5a)互作, 并通过调控ROS稳态参与盐碱胁迫应答(Sun et al., 2016b).此外, GsCBRLK的N端可变域也能与GsPM30及多个Group 3 LEA (late-embryogenesis abundant protein)蛋白互作, GsPM30在拟南芥中过表达会增强幼苗期和成苗期对高盐和脱水的耐性(Sun et al., 2019).GsCPI14 (Glycine soja cystatin protein 14)编码一个蛋白酶抑制剂, 正调控植物的耐碱性(Sun et al., 2014b).另外, Sun等(2013b)获得1个受ABA、盐和干旱胁迫诱导表达的G型凝集素RLK基因GsSRK.该基因过表达促进转基因拟南芥盐胁迫下的种子萌发、幼苗生长和种子产量, 且当缺失N端信号肽和G型凝集素结构域的截短型GsSRK (GsSRK-t)转入苜蓿, 苜蓿的分枝和盐胁迫下的生物量积累增多. ...
... cystatin protein 14)编码一个蛋白酶抑制剂, 正调控植物的耐碱性(Sun et al., 2014b).另外, Sun等(2013b)获得1个受ABA、盐和干旱胁迫诱导表达的G型凝集素RLK基因GsSRK.该基因过表达促进转基因拟南芥盐胁迫下的种子萌发、幼苗生长和种子产量, 且当缺失N端信号肽和G型凝集素结构域的截短型GsSRK (GsSRK-t)转入苜蓿, 苜蓿的分枝和盐胁迫下的生物量积累增多. ...
GsSRK, a G-type lectin S-receptor- like serine/threonine protein kinase, is a positive regulator of plant tolerance to salt stress 2 2013
... Protein kinases implicated in stress tolerance of wild soybean Table 1
编号
基因
编码蛋白
胁迫类型
参考文献
1
GsLRPK
LRR类受体蛋白激酶
冷、干旱、盐和ABA胁迫
杨靓等, 2012; Yang et al., 2014
2
GsRLCK
胞浆类受体蛋白激酶
ABA、盐、碱和干旱胁迫
Sun et al., 2013a
3
GsCBRLK
Ca2+/CaM结合类受体蛋白激酶
冷、盐、干旱和ABA胁迫
Yang et al., 2010; Bai et al., 2013; 赵阳等, 2014
4
GsSRK
G型凝集素类受体蛋白激酶
ABA、盐和干旱胁迫
Sun et al., 2013b, 2018
5
GsAPK
不依赖Ca2+的丝/苏氨酸类蛋白 激酶
冷、盐、干旱和ABA胁迫
Yang et al., 2012
6
GsPPCK1
磷酸烯醇式丙酮酸羧化酶激酶
碱胁迫
魏正巍等, 2013
7
GsPPCK3
磷酸烯醇式丙酮酸羧化酶激酶
碱胁迫
Sun et al., 2014a
胞质类受体蛋白激酶RLCKs (receptor-like cytoplasmic kinases)是一类特殊的RLKs, 其缺少其它RLKs具有的胞外结构域(表1).Sun等(2013a)分离了1个受逆境胁迫诱导的野生大豆RLCK基因GsRLCK, 研究发现该基因在拟南芥中过表达可降低对ABA的敏感性, 提高耐盐性和耐旱性.Yang等(2010)从野生大豆中分离了1个Ca2+/CaM结合的RLK基因GsCBRLK, 其表达受冷、盐、干旱和ABA诱导.GsCBRLK过表达可显著提高转基因拟南芥、苜蓿(Medicago sativa)和大豆对ABA、高盐和碱胁迫的耐受性(Bai et al., 2013; 赵阳等, 2014; Ji et al., 2016b).后续, Sun等 (2014b, 2016b, 2019, 2021)进一步鉴定获得了4个GsCBRLK互作蛋白.其中, GsBET11a编码1个SNARE转运蛋白, 通过C端跨膜结构域与GsCBRLK互作, GsBET11a过表达可提高转基因拟南芥和大豆的耐盐性(Sun et al., 2021).GsCBRLK通过N端可变结构域与GsMSRB5a (methionine sulfoxide reductase B5a)互作, 并通过调控ROS稳态参与盐碱胁迫应答(Sun et al., 2016b).此外, GsCBRLK的N端可变域也能与GsPM30及多个Group 3 LEA (late-embryogenesis abundant protein)蛋白互作, GsPM30在拟南芥中过表达会增强幼苗期和成苗期对高盐和脱水的耐性(Sun et al., 2019).GsCPI14 (Glycine soja cystatin protein 14)编码一个蛋白酶抑制剂, 正调控植物的耐碱性(Sun et al., 2014b).另外, Sun等(2013b)获得1个受ABA、盐和干旱胁迫诱导表达的G型凝集素RLK基因GsSRK.该基因过表达促进转基因拟南芥盐胁迫下的种子萌发、幼苗生长和种子产量, 且当缺失N端信号肽和G型凝集素结构域的截短型GsSRK (GsSRK-t)转入苜蓿, 苜蓿的分枝和盐胁迫下的生物量积累增多. ...
... 胞质类受体蛋白激酶RLCKs (receptor-like cytoplasmic kinases)是一类特殊的RLKs, 其缺少其它RLKs具有的胞外结构域(表1).Sun等(2013a)分离了1个受逆境胁迫诱导的野生大豆RLCK基因GsRLCK, 研究发现该基因在拟南芥中过表达可降低对ABA的敏感性, 提高耐盐性和耐旱性.Yang等(2010)从野生大豆中分离了1个Ca2+/CaM结合的RLK基因GsCBRLK, 其表达受冷、盐、干旱和ABA诱导.GsCBRLK过表达可显著提高转基因拟南芥、苜蓿(Medicago sativa)和大豆对ABA、高盐和碱胁迫的耐受性(Bai et al., 2013; 赵阳等, 2014; Ji et al., 2016b).后续, Sun等 (2014b, 2016b, 2019, 2021)进一步鉴定获得了4个GsCBRLK互作蛋白.其中, GsBET11a编码1个SNARE转运蛋白, 通过C端跨膜结构域与GsCBRLK互作, GsBET11a过表达可提高转基因拟南芥和大豆的耐盐性(Sun et al., 2021).GsCBRLK通过N端可变结构域与GsMSRB5a (methionine sulfoxide reductase B5a)互作, 并通过调控ROS稳态参与盐碱胁迫应答(Sun et al., 2016b).此外, GsCBRLK的N端可变域也能与GsPM30及多个Group 3 LEA (late-embryogenesis abundant protein)蛋白互作, GsPM30在拟南芥中过表达会增强幼苗期和成苗期对高盐和脱水的耐性(Sun et al., 2019).GsCPI14 (Glycine soja cystatin protein 14)编码一个蛋白酶抑制剂, 正调控植物的耐碱性(Sun et al., 2014b).另外, Sun等(2013b)获得1个受ABA、盐和干旱胁迫诱导表达的G型凝集素RLK基因GsSRK.该基因过表达促进转基因拟南芥盐胁迫下的种子萌发、幼苗生长和种子产量, 且当缺失N端信号肽和G型凝集素结构域的截短型GsSRK (GsSRK-t)转入苜蓿, 苜蓿的分枝和盐胁迫下的生物量积累增多. ...
Overexpression of GsZFP1 enhances salt and drought tolerance in transgenic alfalfa (Medicago sativa L.) 3 2013
... 已报道的野生大豆转录组测序研究均表明转录因子在野生大豆耐逆过程中发挥重要作用(表2).GsZFP1编码1个缺少N端QALGGH结构域的C2H2型锌指蛋白, 受冷、干旱、ABA和盐诱导表达(罗晓等, 2012; Luo et al., 2012a); 并可通过CBF依赖和CBF不依赖途径提高转基因拟南芥的耐冷性(Luo et al., 2012a); 还通过调控气孔关闭减少水分散失增强转基因拟南芥和苜蓿的抗旱性(Luo et al., 2012a, 2012b; Tang et al., 2013).GsZFP1超表达增强转基因苜蓿的耐盐性(Tang et al., 2013).因此, GsZFP1作为野生大豆耐逆应答信号通路中的关键基因, 需进一步解析其在野生大豆耐逆应答中的分子机制. ...
... 超表达增强转基因苜蓿的耐盐性(Tang et al., 2013).因此, GsZFP1作为野生大豆耐逆应答信号通路中的关键基因, 需进一步解析其在野生大豆耐逆应答中的分子机制. ...
... Transcription factor involved in stress tolerance of wild soybean Table 2
编号
基因
编码蛋白
胁迫类型
参考文献
1
GsZFP1
ZFP转录因子
冷、干旱、盐和ABA胁迫
罗晓等, 2012; Luo et al., 2012a, 2012b; Tang et al., 2013
GsAPK, an ABA-activated and calcium-independent SnRK2-type kinase from G. soja, mediates the regulation of plant tolerance to salinity and ABA stress 2 2012
... Protein kinases implicated in stress tolerance of wild soybean Table 1
编号
基因
编码蛋白
胁迫类型
参考文献
1
GsLRPK
LRR类受体蛋白激酶
冷、干旱、盐和ABA胁迫
杨靓等, 2012; Yang et al., 2014
2
GsRLCK
胞浆类受体蛋白激酶
ABA、盐、碱和干旱胁迫
Sun et al., 2013a
3
GsCBRLK
Ca2+/CaM结合类受体蛋白激酶
冷、盐、干旱和ABA胁迫
Yang et al., 2010; Bai et al., 2013; 赵阳等, 2014
4
GsSRK
G型凝集素类受体蛋白激酶
ABA、盐和干旱胁迫
Sun et al., 2013b, 2018
5
GsAPK
不依赖Ca2+的丝/苏氨酸类蛋白 激酶
冷、盐、干旱和ABA胁迫
Yang et al., 2012
6
GsPPCK1
磷酸烯醇式丙酮酸羧化酶激酶
碱胁迫
魏正巍等, 2013
7
GsPPCK3
磷酸烯醇式丙酮酸羧化酶激酶
碱胁迫
Sun et al., 2014a
胞质类受体蛋白激酶RLCKs (receptor-like cytoplasmic kinases)是一类特殊的RLKs, 其缺少其它RLKs具有的胞外结构域(表1).Sun等(2013a)分离了1个受逆境胁迫诱导的野生大豆RLCK基因GsRLCK, 研究发现该基因在拟南芥中过表达可降低对ABA的敏感性, 提高耐盐性和耐旱性.Yang等(2010)从野生大豆中分离了1个Ca2+/CaM结合的RLK基因GsCBRLK, 其表达受冷、盐、干旱和ABA诱导.GsCBRLK过表达可显著提高转基因拟南芥、苜蓿(Medicago sativa)和大豆对ABA、高盐和碱胁迫的耐受性(Bai et al., 2013; 赵阳等, 2014; Ji et al., 2016b).后续, Sun等 (2014b, 2016b, 2019, 2021)进一步鉴定获得了4个GsCBRLK互作蛋白.其中, GsBET11a编码1个SNARE转运蛋白, 通过C端跨膜结构域与GsCBRLK互作, GsBET11a过表达可提高转基因拟南芥和大豆的耐盐性(Sun et al., 2021).GsCBRLK通过N端可变结构域与GsMSRB5a (methionine sulfoxide reductase B5a)互作, 并通过调控ROS稳态参与盐碱胁迫应答(Sun et al., 2016b).此外, GsCBRLK的N端可变域也能与GsPM30及多个Group 3 LEA (late-embryogenesis abundant protein)蛋白互作, GsPM30在拟南芥中过表达会增强幼苗期和成苗期对高盐和脱水的耐性(Sun et al., 2019).GsCPI14 (Glycine soja cystatin protein 14)编码一个蛋白酶抑制剂, 正调控植物的耐碱性(Sun et al., 2014b).另外, Sun等(2013b)获得1个受ABA、盐和干旱胁迫诱导表达的G型凝集素RLK基因GsSRK.该基因过表达促进转基因拟南芥盐胁迫下的种子萌发、幼苗生长和种子产量, 且当缺失N端信号肽和G型凝集素结构域的截短型GsSRK (GsSRK-t)转入苜蓿, 苜蓿的分枝和盐胁迫下的生物量积累增多. ...
... 研究人员还发现了其它参与逆境应答的野生大豆蛋白激酶基因(表1), 如GsAPK (Yang et al., 2012)、GsPPCK1 (魏正巍等, 2013)和GsPPCK3 (Sun et al., 2014a).需要指出的是, 大豆4.67% (约2 166个)基因编码蛋白激酶(Liu et al., 2015b).虽然已报道了多个调控野生大豆耐逆性的蛋白激酶, 但对蛋白激酶调控的耐逆性分子机制知之甚少, 尚待进一步探索. ...
GsCBRLK, a calcium/calmodulin-binding receptor-like kinase, is a positive regulator of plant tolerance to salt and ABA stress 2 2010
... Protein kinases implicated in stress tolerance of wild soybean Table 1
编号
基因
编码蛋白
胁迫类型
参考文献
1
GsLRPK
LRR类受体蛋白激酶
冷、干旱、盐和ABA胁迫
杨靓等, 2012; Yang et al., 2014
2
GsRLCK
胞浆类受体蛋白激酶
ABA、盐、碱和干旱胁迫
Sun et al., 2013a
3
GsCBRLK
Ca2+/CaM结合类受体蛋白激酶
冷、盐、干旱和ABA胁迫
Yang et al., 2010; Bai et al., 2013; 赵阳等, 2014
4
GsSRK
G型凝集素类受体蛋白激酶
ABA、盐和干旱胁迫
Sun et al., 2013b, 2018
5
GsAPK
不依赖Ca2+的丝/苏氨酸类蛋白 激酶
冷、盐、干旱和ABA胁迫
Yang et al., 2012
6
GsPPCK1
磷酸烯醇式丙酮酸羧化酶激酶
碱胁迫
魏正巍等, 2013
7
GsPPCK3
磷酸烯醇式丙酮酸羧化酶激酶
碱胁迫
Sun et al., 2014a
胞质类受体蛋白激酶RLCKs (receptor-like cytoplasmic kinases)是一类特殊的RLKs, 其缺少其它RLKs具有的胞外结构域(表1).Sun等(2013a)分离了1个受逆境胁迫诱导的野生大豆RLCK基因GsRLCK, 研究发现该基因在拟南芥中过表达可降低对ABA的敏感性, 提高耐盐性和耐旱性.Yang等(2010)从野生大豆中分离了1个Ca2+/CaM结合的RLK基因GsCBRLK, 其表达受冷、盐、干旱和ABA诱导.GsCBRLK过表达可显著提高转基因拟南芥、苜蓿(Medicago sativa)和大豆对ABA、高盐和碱胁迫的耐受性(Bai et al., 2013; 赵阳等, 2014; Ji et al., 2016b).后续, Sun等 (2014b, 2016b, 2019, 2021)进一步鉴定获得了4个GsCBRLK互作蛋白.其中, GsBET11a编码1个SNARE转运蛋白, 通过C端跨膜结构域与GsCBRLK互作, GsBET11a过表达可提高转基因拟南芥和大豆的耐盐性(Sun et al., 2021).GsCBRLK通过N端可变结构域与GsMSRB5a (methionine sulfoxide reductase B5a)互作, 并通过调控ROS稳态参与盐碱胁迫应答(Sun et al., 2016b).此外, GsCBRLK的N端可变域也能与GsPM30及多个Group 3 LEA (late-embryogenesis abundant protein)蛋白互作, GsPM30在拟南芥中过表达会增强幼苗期和成苗期对高盐和脱水的耐性(Sun et al., 2019).GsCPI14 (Glycine soja cystatin protein 14)编码一个蛋白酶抑制剂, 正调控植物的耐碱性(Sun et al., 2014b).另外, Sun等(2013b)获得1个受ABA、盐和干旱胁迫诱导表达的G型凝集素RLK基因GsSRK.该基因过表达促进转基因拟南芥盐胁迫下的种子萌发、幼苗生长和种子产量, 且当缺失N端信号肽和G型凝集素结构域的截短型GsSRK (GsSRK-t)转入苜蓿, 苜蓿的分枝和盐胁迫下的生物量积累增多. ...
... 胞质类受体蛋白激酶RLCKs (receptor-like cytoplasmic kinases)是一类特殊的RLKs, 其缺少其它RLKs具有的胞外结构域(表1).Sun等(2013a)分离了1个受逆境胁迫诱导的野生大豆RLCK基因GsRLCK, 研究发现该基因在拟南芥中过表达可降低对ABA的敏感性, 提高耐盐性和耐旱性.Yang等(2010)从野生大豆中分离了1个Ca2+/CaM结合的RLK基因GsCBRLK, 其表达受冷、盐、干旱和ABA诱导.GsCBRLK过表达可显著提高转基因拟南芥、苜蓿(Medicago sativa)和大豆对ABA、高盐和碱胁迫的耐受性(Bai et al., 2013; 赵阳等, 2014; Ji et al., 2016b).后续, Sun等 (2014b, 2016b, 2019, 2021)进一步鉴定获得了4个GsCBRLK互作蛋白.其中, GsBET11a编码1个SNARE转运蛋白, 通过C端跨膜结构域与GsCBRLK互作, GsBET11a过表达可提高转基因拟南芥和大豆的耐盐性(Sun et al., 2021).GsCBRLK通过N端可变结构域与GsMSRB5a (methionine sulfoxide reductase B5a)互作, 并通过调控ROS稳态参与盐碱胁迫应答(Sun et al., 2016b).此外, GsCBRLK的N端可变域也能与GsPM30及多个Group 3 LEA (late-embryogenesis abundant protein)蛋白互作, GsPM30在拟南芥中过表达会增强幼苗期和成苗期对高盐和脱水的耐性(Sun et al., 2019).GsCPI14 (Glycine soja cystatin protein 14)编码一个蛋白酶抑制剂, 正调控植物的耐碱性(Sun et al., 2014b).另外, Sun等(2013b)获得1个受ABA、盐和干旱胁迫诱导表达的G型凝集素RLK基因GsSRK.该基因过表达促进转基因拟南芥盐胁迫下的种子萌发、幼苗生长和种子产量, 且当缺失N端信号肽和G型凝集素结构域的截短型GsSRK (GsSRK-t)转入苜蓿, 苜蓿的分枝和盐胁迫下的生物量积累增多. ...
GsLRPK, a novel cold-activated leucine-rich repeat receptor-like protein kinase from Glycine soja, is a positive regulator to cold stress tolerance 1 2014
... Protein kinases implicated in stress tolerance of wild soybean Table 1
编号
基因
编码蛋白
胁迫类型
参考文献
1
GsLRPK
LRR类受体蛋白激酶
冷、干旱、盐和ABA胁迫
杨靓等, 2012; Yang et al., 2014
2
GsRLCK
胞浆类受体蛋白激酶
ABA、盐、碱和干旱胁迫
Sun et al., 2013a
3
GsCBRLK
Ca2+/CaM结合类受体蛋白激酶
冷、盐、干旱和ABA胁迫
Yang et al., 2010; Bai et al., 2013; 赵阳等, 2014
4
GsSRK
G型凝集素类受体蛋白激酶
ABA、盐和干旱胁迫
Sun et al., 2013b, 2018
5
GsAPK
不依赖Ca2+的丝/苏氨酸类蛋白 激酶
冷、盐、干旱和ABA胁迫
Yang et al., 2012
6
GsPPCK1
磷酸烯醇式丙酮酸羧化酶激酶
碱胁迫
魏正巍等, 2013
7
GsPPCK3
磷酸烯醇式丙酮酸羧化酶激酶
碱胁迫
Sun et al., 2014a
胞质类受体蛋白激酶RLCKs (receptor-like cytoplasmic kinases)是一类特殊的RLKs, 其缺少其它RLKs具有的胞外结构域(表1).Sun等(2013a)分离了1个受逆境胁迫诱导的野生大豆RLCK基因GsRLCK, 研究发现该基因在拟南芥中过表达可降低对ABA的敏感性, 提高耐盐性和耐旱性.Yang等(2010)从野生大豆中分离了1个Ca2+/CaM结合的RLK基因GsCBRLK, 其表达受冷、盐、干旱和ABA诱导.GsCBRLK过表达可显著提高转基因拟南芥、苜蓿(Medicago sativa)和大豆对ABA、高盐和碱胁迫的耐受性(Bai et al., 2013; 赵阳等, 2014; Ji et al., 2016b).后续, Sun等 (2014b, 2016b, 2019, 2021)进一步鉴定获得了4个GsCBRLK互作蛋白.其中, GsBET11a编码1个SNARE转运蛋白, 通过C端跨膜结构域与GsCBRLK互作, GsBET11a过表达可提高转基因拟南芥和大豆的耐盐性(Sun et al., 2021).GsCBRLK通过N端可变结构域与GsMSRB5a (methionine sulfoxide reductase B5a)互作, 并通过调控ROS稳态参与盐碱胁迫应答(Sun et al., 2016b).此外, GsCBRLK的N端可变域也能与GsPM30及多个Group 3 LEA (late-embryogenesis abundant protein)蛋白互作, GsPM30在拟南芥中过表达会增强幼苗期和成苗期对高盐和脱水的耐性(Sun et al., 2019).GsCPI14 (Glycine soja cystatin protein 14)编码一个蛋白酶抑制剂, 正调控植物的耐碱性(Sun et al., 2014b).另外, Sun等(2013b)获得1个受ABA、盐和干旱胁迫诱导表达的G型凝集素RLK基因GsSRK.该基因过表达促进转基因拟南芥盐胁迫下的种子萌发、幼苗生长和种子产量, 且当缺失N端信号肽和G型凝集素结构域的截短型GsSRK (GsSRK-t)转入苜蓿, 苜蓿的分枝和盐胁迫下的生物量积累增多. ...
A novel AP2/ERF family transcription factor from Glycine soja, GsERF71, is a DNA binding protein that positively regulates alkaline stress tolerance in Arabidopsis 2 2017
... Transcription factor involved in stress tolerance of wild soybean Table 2
编号
基因
编码蛋白
胁迫类型
参考文献
1
GsZFP1
ZFP转录因子
冷、干旱、盐和ABA胁迫
罗晓等, 2012; Luo et al., 2012a, 2012b; Tang et al., 2013
... TIFY是一类植物特有的新型转录因子, 包含高度保守的TIF[F/Y]XG结构域、GATA锌指结构和Jas结构域.Zhu等(2013)研究发现, 野生大豆包括34个TIFY转录因子, 根据其蛋白序列是否包含GATA锌指结构域分为2类(I和II), 并在NaHCO3胁迫下表现出不同的表达模式.其中, GsTIFY6b、GsTIFY10a、GsJAZ2和GsTIFY11b同时受到NaHCO3与NaCl诱导表达(Zhu et al., 2011, 2012; 朱丹等, 2012; 阎文飞等, 2018).GsTIFY10a在拟南芥和苜蓿中超表达, 一方面上调质子转运相关marker基因(NADP-ME和H+-Ppase)表达, 提高NADP-ME酶活, 增加柠檬酸含量, 以维持胁迫下胞质pH平衡.另一方面上调其它非生物胁迫相关marker基因(RD29A、RD29B、RD22和KIN1)表达, 增加脯氨酸和MDA含量, 提高耐碱性(Zhu et al., 2011, 2014).此外, GsTIFY10a能够形成同源二聚体, 也可与GsTIFY10e形成异源二聚体(Zhu et al., 2014).GsJAZ2和GsTIFY11b通过上调盐胁迫下液泡膜NHX1、质膜SOS1的表达, 提高转基因拟南芥的耐盐性(Zhu et al., 2012; 朱丹等, 2012); 转GsJAZ2基因拟南芥通过促进质子转运相关marker基因的表达提高耐碱性(Zhu et al., 2012). ...
... 形成异源二聚体(Zhu et al., 2014).GsJAZ2和GsTIFY11b通过上调盐胁迫下液泡膜NHX1、质膜SOS1的表达, 提高转基因拟南芥的耐盐性(Zhu et al., 2012; 朱丹等, 2012); 转GsJAZ2基因拟南芥通过促进质子转运相关marker基因的表达提高耐碱性(Zhu et al., 2012). ...
The Arabidopsis kinome: phylogeny and evolutionary insights into functional diversification 1 2014
... 蛋白激酶通过磷酸化下游靶蛋白, 启动或关闭信号转导通路, 调控植物逆境应答.大豆中4.67%的基因编码蛋白激酶(protein kinase), 其中约65%属于类受体蛋白激酶(receptor like kinases, RLKs) (Zulawski et al., 2014; Liu et al., 2015b).RLKs蛋白一般包含1个胞外结构域、1个跨膜结构域和1个胞内激酶结构域. ...
,*黑龙江八一农垦大学, 作物逆境分子生物学实验室, 大庆 163319
