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基于甘蔗产量与土壤磷素平衡的磷肥施用量研究

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区惠平,, 周柳强, 黄金生, 朱晓晖, 曾艳, 彭嘉宇, 谢如林, 谭宏伟,, 李忠宁, 沈小微, 刘昔辉广西壮族自治区农业科学院农业资源与环境研究所/农业农村部华南植物营养与施肥技术科学观测实验站,南宁 530007

Research on Phosphorus Application Rate Based on Sugarcane Yield and Phosphorus Balance in Soil

OU HuiPing,, ZHOU LiuQiang, HUANG JinSheng, ZHU XiaoHui, ZENG Yan, PENG JiaYu, XIE RuLin, TAN HongWei,, LI ZhongNing, SHEN XiaoWei, LIU XiHuiAgricultural Resources and Environmental Research Institute, Guangxi Academy of Agricultural Sciences/South China Scientific Observation and Experiment Station of Plant Nutrition and Fertilization Technology, Ministry of Agriculture and Rural Affairs, Nanning 530007

通讯作者: 谭宏伟,E-mail: hongwei_tan@163.com

责任编辑: 李云霞
收稿日期:2020-08-21修回日期:2020-10-22网络出版日期:2021-07-01
基金资助:国家重点研发项目.2020YFD1000600
国家自然科学基金项目.32060293
国家自然科学基金项目.31860350
国家自然科学基金项目.31750368
国家自然科学基金项目.31860157
国家自然科学基金项目.31860159
广西科技重大专项.桂科AA17204078-2
广西科技重大专项.桂科AA20108002-2
广西科技重大专项.桂AB18221027
广西农科院项目.桂农科2021YT036
广西农科院项目.桂农科2020YM110
广西农科院项目.桂农科2019Z12
广西农科院项目.桂农科2019ZX123
广西农科院项目.桂农科2018YM27
广西农科院项目.桂农科盟202013
农业农村部西南山地农业环境重点实验室开放基金.AESMA-OPP-2019003


Received:2020-08-21Revised:2020-10-22Online:2021-07-01
作者简介 About authors
区惠平,E-mail: ouhuiping2006@163.com













摘要
【目的】探讨南方赤红壤蔗区基于甘蔗产量与土壤磷素平衡的磷肥施用量,为该地区农田磷素高效利用与科学施磷提供参考依据。【方法】于2014—2016年在广西甘蔗主产区(南宁市武鸣区)布置田间定位试验,共设5个磷肥施用量水平,分别是0(P0)、75 kg P2O5·hm-2(P1)、150 kg P2O5·hm-2(P2)、300 kg P2O5·hm-2(P3)和 600 kg P2O5·hm-2(P4),连续3年测定甘蔗蔗茎、蔗叶产量和土壤Olsen-P含量,采用Mitscherlich模型拟合蔗茎产量对Olsen-P的响应曲线,计算土壤Olsen-P农学阈值,并分析植株磷含量,计算甘蔗吸磷量,磷肥利用率和磷素表观平衡状况。【结果】与P1处理相比,P2处理蔗茎产量显著提高8.3%(2014年)、18.0%(2015年)和15.5%(2016年)。蔗叶和地上部产量均以P2或P3处理最高,但不同施磷量间蔗茎、蔗叶和地上部产量整体无显著差异。P2—P4处理蔗茎磷累积量、蔗叶磷累积量和地上部磷累积量也相当。土壤Olsen-P含量、磷素表观平衡量和磷素盈余率均随施磷量的增加而显著增加,而磷素表观回收率和磷素偏生产力随施磷量的增加逐渐下降,以P1处理最高,显著高于P3和P4处理。Mitscherlich方程拟合获得Olsen-P农学阈值为13.4 mg·kg-1。相关分析表明,施磷量与磷素盈余率、磷素盈余率与土壤Olsen-P含量呈极显著的线性正相关关系(P小鱼0.01);磷素盈余率与甘蔗蔗茎产量呈极显著二次相关(P小鱼0.01),与磷素表观回收利用率、磷素偏生产力呈极显著指数相关(P小鱼0.01)。当施磷量为40.9 kg·hm-2时,磷素盈余率为0,土壤Olsen-P含量为15.87 mg·kg-1,甘蔗蔗茎产量为 94.2 t·hm-2。线性加平台拟合下的优化施磷量,土壤磷素盈余率为216.2%—232.7%,土壤Olsen-P含量为24.7—25.4 mg·kg-1,甘蔗蔗茎产量为99.7—100 t·hm-2。【结论】在Olsen-P含量较高的蔗区,40.9 kgP2O5·hm-2施用量能维持土壤磷素平衡,保持土壤适宜的Olsen-P含量,获得较高的产量与磷肥利用率,可以作为推荐的适宜施磷量。
关键词: 甘蔗;磷肥施用量;Olsen-P;磷素平衡

Abstract
【Objective】 This study was conducted to explore the phosphate fertilizer rate based on sugarcane yield and phosphorus (P) balance in soil, with an aim to provide a reference for the efficient utilization and scientific management of P in farmland. 【Method】A 3-year continuous field trail was conducted in Wuming district of Nanning city of Guangxi province from 2014 to 2016, which was designed with five phosphate fertilization treatments, including non-phosphate fertilization (P0), 75 kg P2O5·hm-2 (P1), 150 kg P2O5·hm-2 (P2), 300 kg P2O5·hm-2 (P3) and 600 kg P2O5·hm-2 (P4). The yield of cane and leaves and Olsen-P content in soil were measured, and the relation between cane yield and Olsen-P was evaluated by Mitscherlich model. The agronomic threshold of soil Olsen-P was also calculated. Plant P content, P uptake, P utilization efficiency and P balance in soil were further analyzed. 【Result】Compared with P0 treatment, the cane yield was significantly increased by 8.3% (2014), 18.0% (2015) and 15.5% (2016) under P2 treatment. P2 or P3 treatments had the highest yields for leaves and above-ground part, but there was no significant difference in cane, leaves and above-ground part among different P application rates. P accumulations in cane, leaves and above were also similar among P2 to P4 treatments. Olsen-P content, P balance and P surplus rate increased significantly with the increase of P application rate, while the PRE and PPFP decreased gradually with the increase of P application rate; where P1 treatment was the highest, which was significantly higher than that under P3 and P4 treatments. The agronomic threshold of Olsne-P was 13.4 mg kg-1 based on Mitscherlich model. Correlation analysis showed that P surplus rate was significantly positively correlated with P application rate and soil Olsen-P (P<0.01), and which was significantly quadratic correlated with sugarcane stem yield (P<0.01), and significantly exponential correlated with PRE and PPFP (P<0.01). When P application rate was 40.9 kg·hm-2, P surplus rate, soil Olsen-P content and cane yield were 0,15.87 mg kg-1and 94.2 t·hm-2, respectively. While the maximum cane yield was obtained by linear and platform fitting, soil P surplus rate, Olsen-P content and cane yield were 216.2%-232.7%, 24.7-25.4 mg·kg-1 and 99.7-100 t·hm-2, respectively. 【Conclusion】 In lateritic red soil with relatively high Olsen-P content, the P application rate of 40.9 kg·hm-2would maintain the soil Olsen P content, meet the demand for high yield and high phosphorus utilization efficiency. Thus, it could be used as the recommended P application amount.
Keywords:sugarcane;phosphorus application rate;Olsen-P;phosphorus balance


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本文引用格式
区惠平, 周柳强, 黄金生, 朱晓晖, 曾艳, 彭嘉宇, 谢如林, 谭宏伟, 李忠宁, 沈小微, 刘昔辉. 基于甘蔗产量与土壤磷素平衡的磷肥施用量研究[J]. 中国农业科学, 2021, 54(13): 2818-2829 doi:10.3864/j.issn.0578-1752.2021.13.011
OU HuiPing, ZHOU LiuQiang, HUANG JinSheng, ZHU XiaoHui, ZENG Yan, PENG JiaYu, XIE RuLin, TAN HongWei, LI ZhongNing, SHEN XiaoWei, LIU XiHui. Research on Phosphorus Application Rate Based on Sugarcane Yield and Phosphorus Balance in Soil[J]. Scientia Acricultura Sinica, 2021, 54(13): 2818-2829 doi:10.3864/j.issn.0578-1752.2021.13.011


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0 引言

【研究意义】施用磷肥是保障作物产量的重要措施。但磷肥进入土壤后,易被土壤吸附、固持,形成难以被植物吸收利用的磷酸盐累积在土壤中[1],造成磷肥利用率降低[2]。目前,磷肥过量施用造成土壤磷素累积的现象在全球十分普遍[3],尤以中国较为严重,土壤磷累积以年均11%的增长速率在递增[4]。过量施磷对作物增产作用不明显[5],还可能导致水体污染等环境问题[6]。因此,研究适宜的磷肥施用量对农业生产具有重要的意义。【前人研究进展】有关磷肥适宜施用量的研究很多,有单一根据作物的农艺性状表现及产量效应确定的磷肥施用量[7,8],也有根据土壤速效磷含量或磷肥利用率调整的磷肥施用量[9,10]。而综合考虑作物高产、肥料高效、土壤培肥和环境友好等因素的磷肥推荐方法更符合当前养分管理目标由单一增产向高产、环保、优质等多目标综合的变化[11]。众多研究表明,土壤速效磷含量与施磷量及土壤磷盈亏呈显著正相关关系[12,13,14]。土壤每盈余磷100 kg·hm-2,土壤速效磷可提升1.44—5.74 mg·kg-1[15]。适当的磷盈余有助于土壤生产力和作物生长,然而,过度的磷盈余增加磷流失风险。需要根据不同类型土壤磷素平衡关系、作物产量适当地调整磷肥投入量。吴启华等[16]在有效磷含量较高的黑土区,综合作物产量、磷素吸收、磷肥利用效率和土壤表观磷平衡、有效磷含量,认为施用常规磷水平的80%作为黑土区玉米生产的推荐施磷水平。【本研究切入点】甘蔗是我国最主要的糖料作物,其种植面积占我国常年糖料作物种植面积的85%以上,产糖量占食糖总产量的90%以上[17]。甘蔗产量的稳定增长是保障我国食糖安全的根本。然而,当前甘蔗种植区的滥施、乱施磷肥问题仍较突出,多数蔗区磷肥施入量高达465—513 kg P2O5·hm-2[18],而每吨原料蔗的磷素吸收量仅为0.27—0.7 kg[19]。目前国内外****对甘蔗适宜施磷量的确定大多基于甘蔗的产量效应、生长农艺性状、土壤速效磷含量等某些单一指标[7-8,20-22],综合蔗田磷素收支平衡、甘蔗产量、磷素吸收利用与土壤磷素含量的甘蔗适宜施用量鲜见报道。【拟解决的关键问题】本研究通过3年定位试验系统研究不同施磷量下赤红壤蔗区甘蔗蔗茎产量、蔗叶产量、磷素吸收利用、土壤Olsen-P含量、农学阈值和磷素表观平衡状况的综合变化,旨在探讨维持甘蔗高产的前提下,确定节约高效型施磷量,以期为赤红壤蔗区磷肥科学施用提供科学依据。

1 材料与方法

1.1 试验区概况

本试验于2014—2016年在农业农村部华南植物营养与施肥技术科学观测实验站内(东经108°2'50.2",北纬23°14'49.0",海拔高度115 m)进行。该地属南亚热带湿润季风气候,年平均气温21.7℃,最高气温40.7℃,≥5℃积温8 046 ℃,年均降雨量1 250 mm,年蒸发量892.6 mm,无霜期约为346 d,年均日照时数1 660 h,太阳辐射量为4529 MJ·m-2。温、光、热资源丰富。供试土壤为第四纪红土发育的赤红壤,试验前0—20 cm土层土壤容重1.24 g·cm-3,pH (H2O) 5.32,有机质16.5 g·kg-1,全氮0.9 g·kg-1,全磷 0.55 g·kg-1,全钾4.5 g·kg-1,碱解氮70 mg·kg-1,Olsen-P 19 mg·kg-1,铵态氮4.67 mg·kg-1,硝态氮4.33 mg·kg-1

1.2 试验设计

设置5个磷水平:(1)不施磷肥(P0);(2)75 kg P2O5·hm-2(P1);(3)150 kg P2O5·hm-2(P2);(4)300 kg P2O5·hm-2(P3);(5)600 kg P2O5·hm-2(P4)。所有处理氮钾肥施用量相同,分别为420 kg N·hm-2和300 kg K2O·hm-2。随机区组设计,3次重复,小区面积30 m2(长10 m,宽3 m),共15个小区。试验种植的甘蔗品种为新台糖22,种植制度为1年新植蔗,2年宿根蔗,其中,2014年为新植年份,2015年为第一年宿根,2016为第二年宿根。甘蔗种植及施肥方法参见朱晓晖等[22]文献。杂草与病虫害防治与当地甘蔗种植一致。

1.3 样品采集与测定方法

甘蔗产量:各小区单独测产,在甘蔗收获期将各小区的甘蔗全部平地收获,脱叶,砍去尾稍,按实收甘蔗茎数测产蔗茎产量与蔗叶鲜产量。

植株样品:在甘蔗收获前取小区生长势一致的代表性植株6株,平地收获,分为甘蔗茎和蔗叶两部分。105℃杀青30 min后,70℃烘干至恒重,测定干重,粉碎后用H2SO4-H2O2消化,钼锑抗比色法测磷[23]

土壤样品:每年甘蔗收获后使用直径2 cm的土钻,按X方式采集0—20 cm 土层土壤20个点混合样,室内风干,磨细过0.25 mm筛,碳酸氢钠浸提-钼锑抗比色法测定Olsen-P含量[23]

1.4 数据处理方法[24,25,26,27]

甘蔗产量采用线性加平台模型进行拟合,公式如下:

y=a+bxx<m

y=pxm

式中,y为甘蔗产量(t·hm-2),x为施磷量(kg P2O5·hm-2),a为截距,b为回归系数,m为直线与平台的交点,p为平台最高产量(t·hm-2)。

甘蔗吸磷量(kg·hm-2)=蔗茎产量(kg·hm-2)×蔗茎含磷量(%)+蔗叶产量(kg·hm-2)×蔗叶含磷量(%)

磷素表观回收率(%)=[施磷区甘蔗地上部吸磷量(kg·hm-2)-不施磷区甘蔗地上部吸磷量(kg·hm-2)]/施磷量(kg·hm-2)×100

磷素偏生产力(kg·kg-1)=施磷区产量(kg·hm-2)/施磷量(kg·hm-2

磷素表观平衡(kg·hm-2)=施磷量(kg·hm-2)-甘蔗地上部(蔗茎+蔗叶)吸磷量(kg·hm-2

磷素盈余率(%)=磷素表观平衡(kg·hm-2)/甘蔗地上部吸磷量(kg·hm-2)×100

甘蔗相对产量Yr(t·hm-2)=Yi/Ym×100

作物相对产量对土壤Olsen-P的响应关系通过Mitscherlich方程模拟,公式如下:

Y= A×(1-e-bx

由方程模拟出的相对产量为最大值的90%时,土壤Olsen-P的含量为农学阈值。式中,Yi为每年各处理蔗茎产量(t·hm-2);Ym为每年各处理的最大蔗茎产量(t·hm-2);Y是预测的相对产量;A是最大的相对产量;b是产量对土壤Olsen-P的响应系数。

数据采用Excel 2007进行整理,DPS 7.5 软件和SAS软件分析,Sigmaplot软件和Excel作图。不同处理间多重比较采用Duncan新复极差法(α=0.05)。

2 结果

2.1 不同施磷量对甘蔗产量的影响

表1表明,与P0处理相比,P1处理的蔗茎、蔗叶与地上部产量均无显著变化。当施磷量等于150 kg P2O5·hm-2(P2处理)时,蔗茎产量显著提高8.3%(2014年)、18.0%(2015年)和15.5%(2016年)。但P2—P4处理间无显著差异。蔗叶和地上部产量均以P2或P3处理最高,并于2015年和2016年显著高于P0处理。采用线性加平台模型拟合施磷量与蔗茎、蔗叶、地上部产量的关系得出,3年平均优化施磷量分别为140.2、147.8和142.3 kg P2O5·hm-2,获得最高蔗茎、蔗叶和地上部产量分别为102.6、22.9和125.5 t·hm-2

Table 1
表1
表1不同施磷量对甘蔗产量的影响
Table 1Effect of phosphorus application rate on sugarcane yield
年份
Year
处理
Treatment
产量
Yield (t·hm-2)
优化施磷量
P application rate (kg P2O5·hm-2)
线性加平台最高产量
Plateau (t·hm-2)
蔗茎
Cane
蔗叶
Leaf
地上部
Aerial part
蔗茎
Cane
蔗叶
Leaf
地上部
Aerial part
蔗茎
Cane
蔗叶
Leaf
地上部
Aerial part
2014P092.2±1.6 b21.0±0.5 a113.2±2.1b15015015098.821.1119.9
P195.0±1.6 ab20.9±0.2 a115.9±1.7ab
P299.9±2.3 a21.4±0.5 a121.3±2.0 a
P399.1±2.5 ab21.0±0.3 a120.1±2.8 ab
P498.5±1.6 ab20.9±0.7 a119.4±2.2 ab
2015P089.5±2.7 b20.6± 0.8 b110.1±3.5 b109.9116.6110.6106.422.6129.0
P1101.1±5.7 ab21.9±0.2 ab123.0±5.8 ab
P2105.6±3.1 a23.0±0.3 a128.6±3.5 a
P3108.6±0.6 a22.9±0.7 a131.5±0.5 a
P4105.1±3.8 a21.9±0.8 ab127.0±4.1 a
2016P088.3±1.1 b16.9±0.4 c105.2±1.4 c160.8176.7166.3102.625.1127.7
P194.0±2.7 ab19.2±0.1 bc113.2±2.6 bc
P2102.0±1.2 a24.3±1.3 ab126.3±2.5 ab
P3102.3±5.1 a26.9±1.6 a129.2±6.5 a
P4102.9±4.1 a23.3±2.5 ab126.3±6.6 ab
平均 Average140.2147.8142.3102.622.9125.5
同年份同列数据后不同小写字母表示处理间差异显著(P<0.05)
Different small letters in the same column and the same year meant significant difference between treatments at 5% level

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2.2 不同施磷量对土壤Olsen-P含量的影响

图1表明,施磷显著增加耕层土壤Olsen-P含量,且随施磷量的增加呈线性显著增加(P<0.01,R2=0.960(2014),R2=0.996(2015),R2=0.998(2016)),土壤Olsen-P增加速率随年份的增加而增加,在2014、2015和2016年依次为0.048、0.082和0.131 mg·kg-1

图1

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图1不同施磷量对土壤Olsen-P含量的影响

同一年份中不同的小写字母表示处理间差异显著(P<0.05)。下同
Fig. 1Effects of different phosphorus application rates on soil Olsen-P

The different small letters in the same year indicate significant difference between treatments at 0.05 level. The same as below


2.3 蔗茎产量对土壤Olsen-P的响应

磷农学阈值是指当土壤中的Olsen-P含量达到某个值后,继续施用磷肥,对提高作物的产量作用很小[28]图2显示,以Mitscherlich方程拟合甘蔗蔗茎相对产量和土壤Olsen-P的关系获得的赤红壤蔗地土壤Olsen-P农学阈值为13.4 mg·kg-1

图2

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图2甘蔗相对产量对土壤有效磷的响应关系

Fig. 2Response of relative yield of cane to soil Olsen-P content



2.4 不同施磷量对甘蔗吸磷量的影响

表2显示,与P0处理相比,P1处理对蔗茎磷累积量无显著影响,当施磷量大于150 kg P2O5·hm-2(P2处理)时,蔗茎磷累积量和地上部磷累积量增幅均达显著水平(P<0.05),而P2—P4处理间蔗茎磷累积量、蔗叶磷累积量和地上部磷累积量均差异不显著。

Table 2
表2
表2不同施磷量对甘蔗吸磷量的影响
Table 2Effect of phosphorus application rate on sugarcane P accumulation
年份
Year
处理
Treatment
蔗茎磷累积量
Cane P accumulation (kg·hm-2)
蔗叶磷累积量
Leaves P accumulation (kg·hm-2)
地上部磷累积量
Aboveground P accumulation (kg·hm-2)
2014P011.2±0.59 b5.7±0.31 b16.9±0.87 c
P111.7±0.39 b8.0±0.13 a19.6±0.52 b
P213.3±0.69 a8.3±0.08 a21.6±0.69 a
P313.5±0.24 a7.8±0.23 a21.3±0.41 a
P414.1±0.46 a7.9±0.24 a22.0±0.23 a
2015P08.5±0.44 b6.2±0.62 b14.7±0.90 b
P110.4±0.21 ab8.0±0.05 ab18.4±0.22 a
P212.3±0.29 a7.1±0.39 ab19.4±0.51 a
P312.5±0.62 a8.6±0.48 a21.1±1.11 a
P412.4±1.2 a7.1±0.63 ab19.5±1.34 a
2016P06.9±0.46 b4.6±0.15 c11.5±0.47 c
P19.4±0.18 ab5.4±0.05 bc14.8±0.14 bc
P210.6±0.32 a6.7±0.24 a17.3±0.21 ab
P310.7±1.52 a7.3±0.35 a18.0±1.45 ab
P412.2±0.68 a6.5±0.76 ab18.6±1.39 a

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2.5 不同施磷量对蔗地磷素表观平衡的影响

图3显示,不施磷肥,蔗地磷素表观平衡处于亏缺状态,3年平均亏缺14.4 kg·hm-2。施入磷肥,蔗地磷素表观平衡均处于盈余状态,且随施磷量的增加,磷素表观平衡量和磷素盈余率显著增加。3年平均,施磷处理磷素表观平衡量盈余15.1—241.4 kg·hm-2,盈余率高达88.4%—1218.6%,不同的施磷量间差异显著。

图3

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图3不同施磷量对磷素表观平衡的影响

Fig. 3Effects of different phosphorus application rates on P balance



2.6 不同施磷量对甘蔗磷肥利用效率的影响

磷素表观回收率和磷素偏生产力是衡量和评价磷肥施用经济效益和环境效益的重要指标[29]图4显示,P1处理下,磷素表观回收率最高,3年平均为9.9%,除了2015年,P1处理与P2处理磷素表观回收率均差异不显著,但均显著高于P3和P4处理。磷素偏生产力随施磷量的增加而显著下降。3年平均,P1处理比P2、P3和P4处理显著提高88.6%、274.3%和656.9%。

图4

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图4不同施磷量对磷素表观回收率和磷素偏生产力的影响

Fig. 4Effects of different phosphorus application rates on PRE and PPFP



2.7 磷素盈余率与施磷量、甘蔗蔗茎产量、磷素吸收利用、土壤Olsen-P的关系

磷素盈余率与施磷量、土壤Olsen-P、甘蔗蔗茎产量、磷素吸收利用的回归分析(图5)表明,施磷量与磷素盈余率、磷素盈余率与土壤Olsen-P呈极显著的线性正相关关系(P小鱼0.01);磷素盈余率与甘蔗蔗茎产量呈极显著二次相关(P小鱼0.01);磷素盈余率与磷素表观回收率、磷素偏生产力呈极显著指数相关(P小鱼0.01)。将回归方程联立并通过内插法计算,当磷肥用量为40.9 kg·hm-2,磷素盈余率为0,土壤Olsen-P含量为15.9 mg·kg-1,甘蔗蔗茎产量为94.2 t·hm-2,与P1处理3年平均产量(96.3 t·hm-2)相近。线性加平台拟合下的优化施磷量,磷素盈余率为216.2%—232.7%,土壤Olsen-P含量为24.7—25.4 mg·kg-1,甘蔗蔗茎产量为99.7—100 t·hm-2

图5

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图5磷素盈余率与施磷量、产量、土壤有效磷含量和磷肥利用效率的关系

Fig. 5The correlation of P surplus rate with phosphorus application rate, cane yield, soil Olsen-P and phosphorus utilization efficiency



3 讨论

3.1 适宜的施磷量促进甘蔗增产

作为植物体内ADP、ATP、DNA和细胞壁的主要成分,缺磷会引起作物生长障碍[30],对于甘蔗而言,施磷通过提高甘蔗分蘖和有效茎数[31,32],促进甘蔗增产[32,33,34,35],但过低或过高的施磷量均对作物增产效应不明显[36]。有关甘蔗适宜施磷量的研究,JIMMY等[37]在巴西的试验表明,施磷量为60 kg P2O5·hm-2,蔗茎产量最高(136.5 Mg·hm-2),增幅28.9%。GUSTAVO等[38]研究显示,不同的磷肥种类均在268 kg P2O5·hm-2的施用量下获得最大的甘蔗有效茎数。OMOLLO等[35]的研究结果表明,80 kg P2O5·hm-2下,蔗茎产量最高。而AMIN等[39]研究认为,对于砂壤和黏壤,施入90和180 kg P2O5·hm-2的磷酸盐或过磷酸钙均对甘蔗产量无显著影响。可见,适宜的施磷量因不同生态区域的气候、栽培、肥料种类和土壤条件而异[11]。本研究结果表明,施入150 kg P2O5·hm-2磷肥显著提高甘蔗蔗茎产量,这与雷崇华[21]和朱晓晖等[22]的研究结果一致,当继续增施磷肥,甘蔗蔗茎产量无显著变化,说明本试验地力条件下,150 kg P2O5·hm-2的施磷量即可获得甘蔗最高产。

3.2 施磷量对土壤Olsen-P含量与蔗田磷素表观平衡的影响

植物生长发育吸收的磷素主要来自土壤,而农田生态系统中磷素盈亏是土壤磷消长的根本原因[40]。因此,除了产量、经济效益、肥料利用效率等指标外,探究农田磷素平衡状况及土壤磷素变化对合理施磷量的确定有重要意义[24,41]。多数长期定位研究结果表明,不施磷肥,土壤磷素亏缺进而引起土壤全磷和Olsen-P含量下降[41,42,43]。但也有****[44]认为降雨及灌水中的磷素补充可以维持连续32年不施任何肥料红壤性水稻土的全磷基本持平,Olsen-P略有增加。外源磷的长期过量投入导致土壤磷发生盈余,土壤磷积累[33,45]。本试验与上述结果相似,不施磷肥,蔗田磷素处于亏缺状态,施磷下,土壤磷素处于盈余状态,并随着施磷量的增加而增加。这主要是作物的吸收携出是土壤磷素最主要的支出项[46],在150—600 kg·hm-2的递增施磷下,甘蔗磷素吸收量相当(表2),因此,土壤磷素盈余量随施磷量的增加而大幅度增加,土壤Olsen-P含量也相应增加,这一点从磷的盈余率与施磷量、Olsen-P含量的关系也得到了很好的说明(图5)。然而,土壤Olsen-P含量与作物产量并不是正比关系[47]。Mitscherlich方程拟合甘蔗蔗茎相对产量和土壤Olsen-P的关系表明,当土壤Olsen-P含量超过13.4 mg·kg-1,甘蔗蔗茎产量不再随施磷含量的增加而增加,这与谢如林等[47]研究得出的蔗田土壤Olsen-P丰缺值为14.5 mg·kg-1相近。本研究的试验地,Olsen-P初始含量为19 mg·kg-1,已经超过该地区的Olsen-P农学阈值,施磷处理磷投入足以维持土壤磷素肥力,因此,不同施磷处理间蔗茎产量无显著差异。

3.3 施磷量对磷肥利用的影响

大多数研究表明,磷素表观回收率和磷素偏生产力随磷肥施用量的增加而减少[24],作物获得最高产量的施磷量,其肥料利用率并不一定是最高的[48]。本研究结果表明,虽然P1处理磷素表观回收率和偏生产力最高,但蔗茎产量低于P2处理,因此,如何在维持高产的同时,保障较高的磷肥利用效率,仍是合理施磷的关键。

3.4 磷肥适宜施用量的确定

目前,关于甘蔗磷肥适宜用量的确定大多基于产量与施磷量的关系模型[20,21],确定的甘蔗推荐施磷量虽然能获得最高产量或最佳经济效应,却未必能维持蔗田磷处于平衡状态或最佳含量。将土壤速效磷含量持续控制在临界水平范围内的施肥量是最佳施肥量,且适用于不同肥力水平的土壤[49]。侯云鹏等[24]以理论磷素盈余为0时施磷量的95%置信区间作为磷肥的推荐依据,推荐得出的施磷范围既可保证玉米产量又能维持土壤磷素平衡。本研究结果表明,当施磷量为40.9 kg P2O5·hm-2时,磷素盈余率为0,甘蔗蔗茎产量为94.2 t·hm-2,与P1处理3年平均产量(96.3 t·hm-2)相近,而线性加平台模型拟合的优化施磷量,甘蔗产量仅比磷素盈余率为0的提高5.7%—6.1%,但显著提高土壤Olsen-P含量56%—60%,可能加剧土壤磷素向水体流失的风险。因此,在Olsen-P含量较高的蔗区,可以把40.9 kg P2O5·hm-2施用量作为推荐的适宜施磷量。

4 结论

在广西赤红壤土壤Olsen-P含量较高的蔗区,土壤Olsen-P含量、磷素表观平衡量和磷素盈余率均随施磷量的增加而显著增加,磷素表观回收率和磷素偏生产力随施磷量的增加逐渐下降。40.9 kg P2O5·hm-2的施磷量可维持土壤磷素肥力的同时,获得较高的甘蔗产量和磷素利用率。

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