Soil Ecoenzymatic Stoichiometry and Relationship with Microbial Biomass in Fluvo-Aquic Soils with Various Fertilities
ZHANG Lu,1,2, ZHANG ShuiQing3, REN KeYu2, LI JunJie2, DUAN YingHua,2, XU MingGang,2,41College of Resources and Environment, Jilin Agricultural University, Changchun 130118 2Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences/National Engineering Laboratory for Improving Quality of Arable Land, Beijing 100081 3Institute of Plant Nutrition and Environmental Resources Science, Henan Academy of Agricultural Sciences, Zhengzhou 450002 4South Asian Institute of Tropical Crops, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091,Guangdong
Abstract 【Objective】 Microorganisms and soil enzymes are important drivers for nutrient cycles in farmland soil. To improve the soil fertility and apply fertilizer reasonably, it’s essential to understand the ecoenzymatic stoichiometry of carbon : nitrogen : phosphorus (C : N : P) and quantify its relationship with microbial biomass in soils with various soil fertility levels.【Method】Soil samples were collected from five farmer fields, including high, medium and low fertility levels. Soil type was fluvo-aquic soil. The contents of microbial biomass C and N (SMBC and SMBN), activities of five enzymes and integrated enzyme index (IEI) were analyzed. The enzymes included C-related: β-1, 4-glucosidase (BG) and Cellobiohydrolase (CBH); N-related: β-1, 4-N-acetylglucosaminidase (NAG) and leucine aminopeptidase (LAP); P-related: alkaline phosphatase (AP). The stoichiometry of the enzymes and its relationship with corresponding microbial biomass were studied. 【Result】 The SMBC of high fertility soil was 2.6 and 5.8 times of that in medium and low fertility soil, respectively, and SMBN in high fertility soil was about 3.1 and 5.5 times as much as that in medium and low fertility soils, respectively. There was no significant difference for SMBC/SMBN among the soils. All the five enzyme activities and IEIfollowed the same trend among soils: high fertility > medium fertility > low fertility, and there was a positive linear correlation between C-related/N-related enzymes with SMBC/SMBN. The slope of the relationship indicated that the activity of (BG + CBH) and (NAG + LAP) were increased by 0.134 and 10.53 nmol·g-1·h-1 when SMBC and SMBN were increased by 1 mg·kg-1, respectively. The ratio were lower than 1 both for ln (BG+CBH) : ln (NAG+LAP) and ln (BG+CBH) : ln AP, indicating that the primary limiting factor for nutrient transformation was carbon resource in fluvo-aquic soil. The C : N : P stoichiometry of enzymes in high fertility soil was closer to optimum value than that in low fertility soil.【Conclusion】Application of organic materials, such as manure and straw, would be an efficiency management strategy to promote nutrient cycling by supplement C resources in fluvo-aquic soil. Appropriate ecoenzymatic C : N : P stoichiometry might contribute the high yield and nutrient use efficiency in high fertility soil. Keywords:fluvo-aquic soil;soil fertility;nutrient limitation;eco-stoichiometry;microbial biomass carbon and nitrogen;extracellular enzyme activity
PDF (805KB)元数据多维度评价相关文章导出EndNote|Ris|Bibtex收藏本文 本文引用格式 张露, 张水清, 任科宇, 李俊杰, 段英华, 徐明岗. 不同肥力潮土的酶活计量比特征及其与微生物量的关系[J]. 中国农业科学, 2020, 53(20): 4226-4236 doi:10.3864/j.issn.0578-1752.2020.20.011 ZHANG Lu, ZHANG ShuiQing, REN KeYu, LI JunJie, DUAN YingHua, XU MingGang. Soil Ecoenzymatic Stoichiometry and Relationship with Microbial Biomass in Fluvo-Aquic Soils with Various Fertilities[J]. Scientia Acricultura Sinica, 2020, 53(20): 4226-4236 doi:10.3864/j.issn.0578-1752.2020.20.011
BG+CBH表示与土壤碳转化相关的酶BG和CBH的活性之和,NAG+LAP表示与氮转化相关的酶NAG和LAP的活性之和,AP表示与磷转化相关的酶 Fig. 1Extracellular enzyme activity and integrated enzyme index related to carbon, nitrogen and phosphorus transformation in different fertility soils
BG+CBH represents the sum of the activities of the enzymes BG and CBH related to soil carbon conversion, NAG+LAP represents the sum of the activities of the enzymes NAG and LAP related to nitrogen conversion, and AP represents the enzymes related to phosphorus conversion
BG+CBH表示与土壤碳转化相关的酶BG和CBH的活性之和,NAG+LAP表示与氮转化相关的酶NAG和LAP的活性之和 Fig. 2Relationship between extracellular enzyme activities related to carbon and nitrogen transformation and microbial biomass carbon and nitrogen in different fertility soils
BG+CBH represents the sum of the activities of the enzymes BG and CBH related to soil carbon conversion, NAG+LAP represents the sum of the activities of the enzymes NAG and LAP related to nitrogen conversion
ln(BG+CBH)表示与土壤碳转化相关的酶BG和CBH的活性之和的自然对数,ln(NAG+LAP)表示与氮转化相关的酶NAG和LAP的活性之和的自然对数,lnAP表示与磷转化相关的酶AP的自然对数 Fig. 3Stoichiometry of extracellular enzymes in different fertility soils
ln(BG+CBH) represents the natural logarithm of the sum of the activities of enzymes BG and CBH related to soil carbon conversion, and ln(NAG+LAP) represents the natural logarithm of the sum of the activities of enzymes NAG and LAP related related to nitrogen conversion, lnAP represents the natural logarithm of the enzyme AP associated with phosphorus conversion
BG+CBH表示与土壤碳转化相关的酶BG和CBH的活性之和,NAG+LAP表示与氮转化相关的酶NAG和LAP的活性之和,AP表示与磷转化相关的酶,SOC、TN、TP、TK、Olsen-P、AK、C/N、pH分别表示土壤有机碳、全氮、全磷、全钾、有效磷、有效钾、碳氮比和pH Fig. 4Redundant analysis of extracellular enzyme activity and basic properties of different fertility soils
BG+CBH represents the sum of the activities of the enzymes BG and CBH related to soil carbon conversion, NAG+LAP represents the sum of the activities of the enzymes NAG and LAP related to nitrogen conversion, and AP represents the enzymes related to phosphorus conversion, SOC, TN, TP, TK, Olsen-P, AK, C/N, and pH represent soil organic carbon, total nitrogen, total phosphorus, total potassium, available phosphorus, available potassium, carbon-nitrogen ratio, and pH, respectively
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