Abstract 【Objective】The study was conducted to investigate the effects of spraying growth regulators on the distribution of photosynthetic products in sweet potato and the alleviation effects on drought stress.【Method】Artificial dry shed and dry pond were used to simulate the drought stress of potato swell (100-120 d), and based on 13C labeling method, the alleviation effects of spraying three exogenous hormones such as 6-benzylaminopurine (6-BA), α-naphthyl acetic acid (NAA) and abscisic acid (ABA) on drought stress of sweet potato were studied. The physiological and biochemical indexes, such as the accumulation and distribution rate of 13C, endogenous hormone content, carbon metabolism enzyme activity and photosynthetic fluorescence characteristics of sweet potato leaves, were analyzed. And then we performed stepwise regression analysis, path analysis and RDA analysis.【Result】Compared with normal water supply, drought stress decreased the yield of sweet potato by 18.76% (P<0.05). The degree of the decrease of yield was greatly smaller with the spraying of growth regulator under drought stress (P<0.05). The best effect of spraying was 6-BA which was followed by ABA and NAA. Spraying growth regulators under drought stress could significantly improve the photosynthetic efficiency of functional leaves, which could promote the synthesis of photosynthetic products during the expansion of potato tubers. Compared with the spraying of water, the spraying of 6-BA promoted the net photosynthetic rate (Pn), which increased by 10.93% and the maximum photochemical efficiency (Fv/Fm) which increased by 20.00%. Spraying different growth regulators in drought could significantly increase the instantaneous accumulation of 13C and the rate of distribution to the roots of sweet potato. Different growth regulators could promote the transfer of photosynthetic products ( 13C) from leaves to roots and distribution which increased by 75.68% and 27.68% by spraying 6-BA, respectively. In addition, spraying growth regulators increased leaf carbon metabolizing enzyme activity. Compared with the spraying of water, the spraying of 6-BA, NAA and ABA increased SS enzyme activity increased by 29.59%, 19.25% and 13.03%, respectively. Spraying growth regulators alleviated the decrease of ZR and IAA content caused by drought. Compared with the spraying of water, the spraying of 6-BA increased ZR and IAA by 18.72% and 10.97%, respectively. Stepwise regression analysis showed that photosynthetic characteristics, chlorophyll fluorescence characteristics, carbon metabolism enzyme activity and endogenous hormones were key indicators for regulating the distribution of photosynthetic products ( 13C) at the peak of potato tube expansion (R=0.997); Path analysis showed that Pn, SPS, ABA, ZR, SS and Fv/Fm had the higher direct interaction coefficient on the transfer and distribution of the product ( 13C) from the leaves to the roots for sweet potato photosynthesis; RDA analysis showed that the spraying of 6-BA had a good correlation with the total 13C accumulation of sweet potato, the distribution of 13C in roots, ZR, Pn, Fv/Fm, SS and SPS.【Conclusion】Spraying growth regulators under drought stress raised endogenous hormone content, carbon metabolism enzyme activity based on SPS and ADPGase, improved leaf photosynthetic characteristics, and promoted the transport of photosynthetic products ( 13C) from leaf to root in potato tube expansion stage, besides which could relieve the effects of drought stress. Keywords:sweet potato;drought stress;plant growth regulators;13C distribution
PDF (1103KB)元数据多维度评价相关文章导出EndNote|Ris|Bibtex收藏本文 本文引用格式 王金强, 李思平, 刘庆, 李欢. 喷施生长调节剂缓解甘薯干旱胁迫的机理[J]. 中国农业科学, 2020, 53(3): 500-512 doi:10.3864/j.issn.0578-1752.2020.03.004 WANG JinQiang, LI SiPing, LIU Qing, LI Huan. Mechanism of Spraying Growth Regulators to Alleviate Drought Stress of Sweet Potato[J]. Scientia Acricultura Sinica, 2020, 53(3): 500-512 doi:10.3864/j.issn.0578-1752.2020.03.004
Table 5 表5 表5干旱胁迫下喷施生长调节剂对甘薯SS、SPS和ADPGase酶活性的影响(120 d) Table 5Effects of spraying growth regulators on SS,SPS and ADPGase in sweet potato leaves under drought stress (120 d)
Table 6 表6 表6干旱胁迫下喷施生长调节剂对甘薯叶片内源激素含量的影响 Table 6Effects of spraying growth regulators on endogenous hormones in sweet potato leaves under drought stress
Table 7 表7 表7干旱胁迫下生长调节剂对甘薯膨大期13C分配量与生理指标的通径系数 Table 7Path coefficient between physiological indexes and 13C accumulation of sweet potato by growth regulators during expansion period under drought stress
RDA分析表明前两个排序轴的解释量分别达到69.0%和9.9%,经蒙特卡洛检验P=0.002,达显著性水平 Fig. 2Redundancy analysis between photosynthesis product accumulation and photosynthetic characteristics, endogenous hormones of sweet potato
The coordination from the first two ordination axes was explained by 69.0% and 9.9% of the variance. The significance based on Monte Carlo permutation test of all canonical axes was P=0.002
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