Pyramiding and evaluation of brown planthopper resistance genes in water-saving and drought-resistance restorer line
ZHANG An-Ning1,2, LIU Yi1,2, WANG Fei-Ming1, XIE Yue-Wen2, KONG De-Yan1, NIE Yuan-Yuan3, ZHANG Fen-Yun1, BI Jun-Guo1, YU Xin-Qiao1, LIU Guo-Lan1, LUO Li-Jun,1,2,*1 Shanghai Agrobiological Gene Center, Shanghai 201106, China 2 College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China 3 Jiangxi Super Rice Research and Development Center, Nanchang 330200, Jiangxi, China
This study was supported by the Shanghai Seed Industry Development Project.2019-02-08-00-08-F01110 the Shanghai Agriculture Applied Technology Development Program.2018-02-08-00-08-F01553 the National Key Research and Development Program of China.2017YFD0100304
Abstract The brown planthopper (Nilaparvata lugens St?l, BPH) is the most serious pest threat to rice production across Asia. Increasing host-plant resistance is the most economical and ecological strategy for controlling this pest. The objective of this study was to survey the resistance effects of different R genes to brown planthopper, reveal its influence on agronomic traits, and provide insights into molecular breeding of rice with resistance to brown planthopper. In this research, the brown planthopper R genes Bph6, Bph9, Bph14, and Bph15 were introgressed into the water-saving and drought-resistant rice restorer line ‘Hanhui 3’ through marker-assisted backcrossing scheme. The standard seedling group screening method was used to identify the resistance of the improved lines. Among single-gene improved lines, the order of the R genes effects was Bph9 > Bph6 > Bph15 > Bph14, and among pyramiding improved lines, that was Bph6+Bph9+Bph14+Bph15 > Bph6+Bph9 > Bph6+Bph9+Bph14 > Bph6+Bph9+Bph15 > Bph6+Bph14+Bph15 > Bph9+Bph14+Bph15 > Bph14+Bph15. Furthermore, the survey of agronomic traits demonstrated that there were no significant differences between the 11 improved lines and recurrent parent ‘Hanhui 3’ in plant height, panicle number per plant and 1000-grain weight. These results suggest that the introgression of Bph6, Bph9, Bph14, and Bph15 genes by molecular marker-assisted selection technology could enhance the resistance to brown planthopper and improve breeding efficiency. Keywords:breeding;brown planthopper;resistance gene;marker-assisted selection;resistance evaluation;water-saving and drought-resistance rice
PDF (1247KB)元数据多维度评价相关文章导出EndNote|Ris|Bibtex收藏本文 本文引用格式 张安宁, 刘毅, 王飞名, 谢岳文, 孔德艳, 聂元元, 张分云, 毕俊国, 余新桥, 刘国兰, 罗利军. 节水抗旱稻恢复系的抗褐飞虱分子标记辅助选育及抗性评价[J]. 作物学报, 2019, 45(11): 1764-1769. doi:10.3724/SP.J.1006.2019.82066 ZHANG An-Ning, LIU Yi, WANG Fei-Ming, XIE Yue-Wen, KONG De-Yan, NIE Yuan-Yuan, ZHANG Fen-Yun, BI Jun-Guo, YU Xin-Qiao, LIU Guo-Lan, LUO Li-Jun. Pyramiding and evaluation of brown planthopper resistance genes in water-saving and drought-resistance restorer line[J]. Acta Crops Sinica, 2019, 45(11): 1764-1769. doi:10.3724/SP.J.1006.2019.82066
Table 2 表2 表2改良品系的主要农艺性状 Table 2Agronomic traits of improved lines
编号 No.
基因型 Genotype
株高 Plant height (cm)
单株有效穗 Panicles per plant
穗长 Panicle length (cm)
每穗总粒数 Grains per panicle
结实率 Grain fertility (%)
千粒重 1000-Grain weight (g)
单株产量 Grain yield per plant (g)
18R1
Bph6
121.00±1.00
13.67±3.21
21.49±0.58
200.30±2.84**
83.35±3.04
26.35±0.88
32.34±0.70*
18R2
Bph9
119.68±1.64
9.88±0.55
25.53±0.87**
202.65±5.16**
77.62±1.78
24.79±1.66
33.94±1.74
18R3
Bph14
129.00±6.24
10.00±4.00
24.88±1.80
199.98±7.81**
90.37±1.14
25.00±1.20
29.04±0.73**
18R4
Bph15
120.48±1.36
10.70±1.00
26.13±0.45**
200.22±3.20**
79.16±2.39
24.32±0.54
34.94±1.47
18R5
Bph6+Bph9
120.65±2.67
10.58±0.64
25.75±0.57**
201.66±2.29**
76.68±0.98*
24.44±1.58
31.99±3.46*
18R6
Bph14+Bph15
119.71±2.21
9.88±0.64
26.08±0.49**
198.87±7.43**
80.37±1.66
26.07±1.82
30.64±1.71**
18R7
Bph6+Bph9+Bph14
118.12±1.46
10.50±1.04
24.90±0.48*
194.34±2.80**
71.64±1.30**
26.21±1.12
34.90±0.71
18R8
Bph6+Bph9+Bph15
124.19±0.13
10.04±0.69
25.13±0.85*
205.54±5.21**
71.86±1.73**
24.44±0.88
32.75±2.89
18R9
Bph6+Bph14+Bph15
119.89±1.59
10.95±0.23
24.94±0.61*
200.59±4.13**
68.69±1.13**
24.87±1.73
36.47±1.04
18R10
Bph9+Bph14+Bph15
118.41±3.68
10.99±0.53
26.14±0.62**
201.56±2.44**
69.90±3.57**
26.53±0.52
31.19±1.82*
18R11
Bph6+Bph9+Bph14+Bph15
121.12±2.63
11.33±0.56
25.82±0.36**
200.79±6.06**
57.26±2.73**
26.40±1.21
30.38±1.89**
Hanhui 3
117.00±4.90
10.00±0.82
22.80±0.41
240.69±21.35
86.24±6.49
25.84±1.62
42.11±6.78
The data are the average ± standard error; * indicates significant difference at the 0.05 probability level; ** indicates significant difference at the 0.01 probability level. 数据均为平均值±标准误; *表示与对照旱恢3号在0.05水平显著差异; **表示与对照旱恢3号在0.01水平极显著差异。
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