关键词:烟草; 基因型; 苗期; 氮素; 氮高效 Screening of Tobacco Genotypes Tolerant to Low-Nitrogen and Their Nitrogen Efficiency Types ZHONG Si-Rong1, CHEN Ren-Xiao2, TAO Yao1, GONG Si-Yu1, HE Kuan-Xin2, ZHANG Qi-Ming2, ZHANG Shi-Chuan1, LIU Qi-Yuan1,* 1 Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / Key Laboratory of Crop Physiology, Ecology and Genetic Breeding of Jiangxi Province / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
2Jiangxi Leaf Tobacco Research Institute, Nanchang 330045, China
Fund:This study was supported by the Jiangxi Provincial Tobacco Monopoly Bureau of Science and Technology Project (201401001). AbstractGenotypes with tolerance to low nitrogen (N) are preferred in improving N use efficiency of tobacco and reduce nitrogen pollution. In this study, seedlings of 74 tobacco genotypes were evaluated in a hydroponic experiment with low-N (0.5 mmol L-1) and normal-N (5.0 mmol L-1) treatments, and the genotypes were evaluated according to descriptive statistics, factor analysis and cluster analysis. The root volume, root biomass, N accumulation in stalk and leaf, and aboveground biomass varied greatly across genotypes with the variation coefficients of 0.37-0.68 in low-N treatment and 0.38-0.64 in normal-N treatment. The principal components under both N levels were similar, showing that the N accumulation in stalk and leaf and aboveground biomass were important components. According to evaluation indexes Heatmap clustering and scatter diagram analysis of N efficiency comprehensive value, 15 low-N tolerant genotypes were screened out, accounting for 20.3% of varieties tested, including eight genotypes of high-efficiency under low-N but low-efficiency under normal-N, six genotypes of low-efficiency under both low-N and normal-N, and one genotype of high-efficiency under both low-N and normal-N. In addition, eight genotypes were found to be sensitive to low-N, including six genotypes of low-efficiency under low-N but high-efficiency under normal-N and two genotypes of low-efficiency under both low-N and normal-N. We primarily identified 14P9 as the low-N tolerant and N-efficient tobacco and Zhongyan 100 and K394 as the low-N sensitive and N-inefficient tobacco.
Keyword:Tobacco; Genotype; Seedling stage; Nitrogen; High nitrogen efficiency Show Figures Show Figures
表1 不同供氮水平下烟草苗期性状指标的变化 Table 1 Changes of indicators in tobacco seedlings under different nitrogen levels
指标 Index
低氮 Low nitrogen
正常氮 Normal nitrogen
变幅 Range
均值 Average
标准差 SD
变异系数CV
变幅 Range
均值 Average
标准差 SD
变异系数 CV
SPAD值 SPAD value
9.80-21.47
16.38 aA
1.82
0.11
13.10-30.17
21.30 bB
3.02
0.14
茎叶氮素含量 Stalk and leaf nitrogen content (%)
2.18-4.04
2.86 aA
0.45
0.16
2.90-4.90
3.80 bB
0.35
0.09
地上部生物量 Aboveground biomass per plant (g)
0.08-0.55
0.29 aA
0.10
0.37
0.16-1.04
0.50 bB
0.19
0.38
茎叶氮累积量 Stalk and leaf nitrogen accumulation (mg plant-1)
3.12-16.46
7.96 aA
2.97
0.37
5.98-40.16
19.01 bB
7.48
0.39
根系生物量 Root biomass per plant (g)
0.01-0.10
0.03 aA
0.02
0.57
0.01-0.10
0.03 aA
0.02
0.61
单株叶鲜重 Leaf fresh weight per plant (g)
2.14-9.64
5.10 aA
1.64
0.32
2.13-13.97
7.65 bB
2.51
0.33
单株茎鲜重 Stalk fresh weight per plant (g)
0.41-3.89
1.95 aA
0.78
0.40
0.84-6.53
3.79 bB
1.37
0.36
株高 Plant height (cm)
2.43-24.23
13.45 aA
4.77
0.35
6.57-31.40
18.73 bB
4.94
0.26
茎粗 Stalk diameter (mm)
3.07-5.71
4.32 aA
0.55
0.13
2.93-6.40
4.78 bB
0.72
0.15
根系体积 Root volume (cm3)
0.13-2.63
0.66 aA
0.44
0.68
0.10-1.63
0.54 aA
0.35
0.64
Values followed by different capital and small letters are significantly different at the 0.01 and 0.05 probability level, respectively. SD: standard deviation; CV: coefficient of variation. 标以不同大、小写字母的数值间差异分别达到0.01和0.05显著水平。
表1 不同供氮水平下烟草苗期性状指标的变化 Table 1 Changes of indicators in tobacco seedlings under different nitrogen levels
图1 不同基因型烟草指标相对值聚类图Fig. 1 Heatmap clustering for relative indicatiors in different tobacco genotypes
表3 Table 3 表3(Table 3)
表3 不同供氮条件下烟草苗期地上部生物量和茎叶氮积累量相对值 Table 3 Relative values of aboveground biomass and stalk leaf nitrogen accumulation under different nitrogen levels at seedling stage
品种 Variety
指标相对值Relative value
品种 Variety
指标相对值Relative value
地上部生物量 AB
茎叶氮累积量 SLNA
地上部生物量 AB
茎叶氮累积量 SLNA
安选4号 Anxuan 4
0.38
0.28
Oxford 2028
0.87
1.01
闽烟3号 Minyan 3
0.37
0.33
RG11
0.57
0.36
云选2号 Yunxuan 2
0.41
0.26
RG17
0.71
0.48
大白筋599 Dabaijin 599
0.76
0.50
云烟317 Yunyan 317
0.32
0.18
革新3号 Gexin 3
0.77
0.46
CV70
0.80
0.52
单育2号 Danyu 2
0.43
0.28
抗88 Kang 88
0.74
0.53
净叶黄 Jingyehuang
0.42
0.26
中烟104 Zhongyan 104
0.54
0.38
春雷3号 Chunlei 3
0.69
0.42
中烟101 Zhongyan 101
0.40
0.40
Coker 347
0.90
0.51
鲁烟2号 Luyan 2
0.86
0.57
DB101
0.80
0.54
ZC01
0.40
0.34
NC95
0.76
0.54
14P4
0.94
0.65
G28
0.77
0.50
14P5
0.41
0.26
Ti245
0.98
0.72
14P9
0.82
0.68
Ti448A
0.55
0.34
云烟87 Yunyan 87
0.64
0.50
86-3002
0.36
0.29
14P13
0.47
0.47
永定1号 Yongding 1
0.62
0.45
14P14
0.60
0.55
云烟2号 Yunyan 2
0.88
0.63
CB1
0.33
0.26
Coker 176
0.29
0.18
14P11
0.69
0.46
K326
0.42
0.34
红花大金元Honghuadajinyuan
0.55
0.42
K394
0.19
0.15
14P10
0.81
0.66
K399
0.72
0.42
14P18
0.94
0.66
NC82
0.63
0.51
NX326
0.65
0.46
NC567
0.45
0.32
HY06
0.93
0.73
G80
0.60
0.41
MSB25
0.38
0.44
台烟7号 Taiyan 7
0.69
0.46
MSB47
0.48
0.43
中烟90 Zhongyan 90
0.55
0.46
MSB31
0.60
0.41
CV87
0.29
0.27
MS39
0.68
0.53
云花1号 Yunhua 1
0.46
0.35
MSB52
0.45
0.30
龙岩C2 Longyan C2
0.77
0.48
MSB44
0.52
0.44
Coker 139
0.70
0.42
MSB58
0.63
0.51
中烟98 Zhongyan 98
0.38
0.32
NordelB
0.31
0.28
中烟100 Zhongyan 100
0.21
0.21
MSKCH1
0.77
0.92
G80B
0.66
0.47
云烟97 Yunyan 97
0.58
0.48
岩烟97 Yanyan 97
0.33
0.19
云烟98 Yunyan 98
0.86
0.68
C151
0.80
0.73
云烟99 Yunyan 99
0.56
0.36
96019
0.87
0.92
云烟105 Yunyan 105
0.97
0.55
K346
0.41
0.29
FL57
0.74
0.58
AB: aboveground biomass; SLNA: stalk and leaf nitrogen accumulation.
表3 不同供氮条件下烟草苗期地上部生物量和茎叶氮积累量相对值 Table 3 Relative values of aboveground biomass and stalk leaf nitrogen accumulation under different nitrogen levels at seedling stage
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