关键词:绿豆; 连锁遗传图谱; SSR标记; 复合区间作图法; QTL; 贡献率 Construction of Genetic Map and Identification of QTLs Related to Agronomic Traits in Mung Bean WANG Jian-Hua1,2,3, ZHANG Yao-Wen3, CHENG Xu-Zhen2,*, WANG Li-Xia2,* 1 Shanxi University, Taiyuan 030006, China
2Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
3Institute of Crop Science, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China
Fund:This study was supported by the China Agriculture Research System (CARS-09) and the Agricultural Science and Technology Innovation Program of CAAS AbstractTwo hundreds and eight individuals of F2 population, derived from a cross between two mung bean genotypes (Huaye 1 and Zijing 1) were used to construct genetic map, and to identify QTLs related to important agronomic traits. This genetic map contained 11 linkage groups with a total length of 1457.47 cM and an average interval of 15.34 cM. QTLs mapping was conducted for plant height, young stem color, main stem color, growth habit, podding habit, trilobate leaf shape and mature leaf color using composite interval mapping method. Only one QTL for each trait was detected including plant height, young stem color, main stem color and trilobate leaf shape, and with a contribution ranging from 8.49% to 66.64%. Three QTLs with high contribution rates from 60.32% to 80.36% were identified for the trait of pod habit in mung bean. Four QTLs related to mature leaf color showed at contribution rate from 69.06% to 87.35%. There were 26 QTLs related to growth habit, the most of the tested QTLs, with a contribution rate each from 58.32% to 99.51%. The present QTLs for seven agronomic traits distributed on LG1, LG2, LG4, LG8, and LG10, respectively, could be used in molecular breeding based on marker-assisted selection in mung bean, and also lay a foundation for further study of the inheritance of these traits.
Keyword:Mung bean; Genetic linkage map; SSR markers; Composite interval mapping (CIM); QTL; Contribution rate Show Figures Show Figures
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