关键词:甘蓝型油菜; 角质层蜡质; 水杨酸(SA); 茉莉酸甲酯(MeJA); 乙烯(ETH) Effects of SA, MeJA, and ACC on Leaf Cuticular Wax Constituents, Structure and Permeability in Brassica napus LI Shuai, ZHAO Qiu-Ling, PENG Yang, XU Yi, LI Jia-Na, NI Yu* College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China Fund:This study was supported by the Fundamental Research Funds for the Central Universities (XDJK2014B037), the Chongqing Basic and Advanced Research Project (cstc2016jcyjA0170), and the National Natural Science Foundation of China (31000122). AbstractCuticular waxes are related to plant adaptation to environment stress. In the current study, Brassica napus cv. Zhongshuang 11 grown in the soil treated with adding solutions of SA (200 μmol L-1), MeJA (100 μmol L-1), and ACC (200 μmol L-1) wax, were sampled at the five-leaf stage to clarify the effects of SA, MeJA and ETH on adjusting plant cuticular wax deposition. The leaves were used to analyze the amounts of total cuticular wax and wax constituents, wax crystal structure, and cuticular permeability. The amounts of n-alkanes, secondary alcohols, ketones, aldehydes and total cuticular waxes increased significantly when compared with the control at seven days after MeJA treatment, whereas the amounts of all wax constituents and total cuticular waxes significantly decreased at 14 days after MeJA treatment. The application of SA and ACC had no significant influence on cuticular wax deposition at 7 and 14 days after treatments, except for an amount reduction of primary alcohol, aldehyde and unknown constituents for SA treatment at 14 days after treatment. At 21 days after SA, MeJA, and ACC application, the amounts of total cuticular wax, n-alkanes, ketones and aldehyde significantly increased. C29 n-alkane, C29 ketone, and C30 aldehyde were the main cuticular wax constituents induced by SA, MeJA, and ACC application, implying that n-alkane, ketone and aldehyde might be related to the resistance to stresses induced by these signal molecules. Scan electric microscope analysis indicted that the rod-shape structure of cuticular wax in leaf surface reduced and some rods melted under SA treatment. MeJA and ACC application increased the distribution density of wax crystalloids. The cuticular wax deposition and crystal structure alteration reduced the cuticular permeability and delayed the leaf water loss. The specific increase of C29 n-alkane might be the main reason for reducing leaf water loss in B. napus.
Keyword: Brassica napus L; Cuticular wax; Salicylic acid; Methyl jasmonic acid; Ethylene Show Figures Show Figures
图1 SA、MeJA和ACC处理对油菜叶角质层蜡质主要组分含量的影响 数据柱上* 表示在P < 0.05水平上差异显著。Fig. 1 Effects of SA, MeJA, and ACC treatments on contents of major individual leaf cuticular wax constituents in B. napus Bars with asterisks are significantly different at P < 0.05 according to Student’ s t-test.
图2 SA、MeJA和ACC处理对油菜叶表皮蜡质晶体结构的影响 A~B: 对照; C~D: SA处理; E~F: MeJA处理; G~H: ACC处理; A、C、E、G: 标尺=100 μ m; B、D、F、H: 标尺=10 μ m。Fig. 2 Effects of SA, MeJA, and ACC treatment on the crystalloid structures of leaf cuticular wax in B. napus A-B: control; C-D: SA; E-F: MeJA; G-H: ACC; A, C, E, and G: bar=100 μ m; B, D, F, and H: bar=10 μ m.
4 结论外源SA、MeJA及ACC处理能够诱导甘蓝型油菜中双11叶片角质层蜡质的沉积, 降低角质层渗透性, 减缓叶片的水分散失, 其中C29烷的特异性增加是造成叶片失水率降低的主要原因。蜡质组分中烷类、酮类以及醛类可能与SA、JA和ETH所介导的抗(耐)性反应密切相关。 The authors have declared that no competing interests exist.
作者已声明无竞争性利益关系。The authors have declared that no competing interests exist.
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