关键词:小麦; 揉混参数; 关联分析; 分子标记 Association Analysis for Mixograph Properties in Ningmai 9 and Its Derivatives JIANG Peng, ZHANG Ping-Ping, ZHANG Xu, CHEN Xiao-Lin, MA Hong-Xiang* Jiangsu Academy of Agricultural Sciences / Jiangsu Provincial Key Laboratory for Agrobiology / Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 210014, China Fund:This study was supported by China Agriculture Research System (CARS-3), Jiangsu Provincial Fund for Independent Innovation in Agricultural AbstractMixograph property is one of the rheological properties affecting the quality of end-use products in wheat. Ningmai 9 is a soft wheat cultivar planted largely in the middle and lower reaches of Yangtze River and served as a main parent of 17 released wheat cultivars. For the purpose of utilizing Ningmai 9 in molecular marker-assisted selection, 185 polymorphic SSR markers associated with mixograph properties were tested in Ningmai 9 and its 117 derivatives. The mixed-linear model (MLM) was used in combination of phenotypic data collected in the 2009-2010 and 2010-2011 growing seasons. A total of 13 SSRs were identified to be significantly associated with mixograph properties ( P< 0.01), explaining 5.71-12.33% of phenotypic variations. Among the 13 markers, three, three, two, and seven were associated with mix time (MT), peak height (PH), peak width (PW), eight-minute width (8MW), respectively. Markers Xwmc11, Xbarc320, Xbarc110, and Xgwm577 for 8MW, Xwmc594for MT, and Xgwm299for PW were identified over two years. Besides, Xwmc594 and Xgwm577 were associated with both (MT) and 8MW. The six markers identified over two years all negatively affected mixograph parameters in Ningmai 9 background and might be used in soft wheat breeding.
Keyword:Wheat; Mixograph parameters; Association analysis; Molecular markers Show Figures Show Figures
表1 宁麦 9 号及其衍生系揉混参数的方差分析 Table 1 Analysis of varience in mixograph parameters of Ningmai 9 and its derivatives
年份 Year
宁麦9号衍生系 Derivatives of Ningmai 9
多重比较 Multiple comparison
F值 F-value
均值 Mean
标准差 SD
范围 Range
变异系数 CV (%)
宁麦9号 Ningmai 9
衍生一代 1st generation
衍生二代 2nd generation
基因型 Genotype
年份 Year
和面时间 Mix time (min)
2010
1.36
0.66
0.55-3.79
48.99
1.76 a
1.54 a
1.27 a
44.39* *
0.06
2011
1.35
0.63
0.63-3.81
46.41
1.73 a
1.52 a
1.27 a
峰值高度 Peak height (%)
2010
62.68
6.50
47.01-80.20
10.37
69.43 a
60.77 a
63.63 a
40.29* *
17.09* *
2011
63.46
6.48
46.58-81.90
10.21
68.18 a
61.38 a
64.51 a
峰值宽度 Peak width (%)
2010
40.91
8.74
20.17-58.40
21.36
43.75 a
36.90 a
42.91 a
75.13* *
10.70* *
2011
41.54
8.86
21.09-58.80
21.34
41.56 a
37.51 a
43.55 a
8 min带宽 Eight-minute width (%)
2010
27.34
9.05
12.16-52.02
33.11
25.02 a
24.62 a
28.70 a
72.77* *
3.87
2011
27.74
8.75
13.11-53.02
31.55
22.53 a
24.87 a
29.17 a
The multiple comparison followed by the lowercase indicated significantly different at the 0.05 probability level; The F-value followed by * * indicate significance at the 0.01 probability level. 多重比较数据后小写字母表示在0.05概率水平下的差异显著性; F值后* * 表示0.01概率水平下显著。
表1 宁麦 9 号及其衍生系揉混参数的方差分析 Table 1 Analysis of varience in mixograph parameters of Ningmai 9 and its derivatives
表2 宁麦9号及其衍生系揉混参数的相关系数 Table 2 Correlation coefficients of mixograph parameters in Ningmai 9 and its derivatives
揉混参数 Mixograph parameter
和面时间 Mix time
峰值高度 Peak height
峰值宽度 Peak width
8 min带宽 Eight-minute width
和面时间 Mix time
0.96* *
0.06
-0.27* *
0.17
峰值高度 Peak height
0.04
0.95* *
0.73* *
0.56* *
峰值宽度 Peak width
-0.34* *
0.81* *
0.97* *
0.46* *
8 min带宽 Eight-minute width
0.13
0.58* *
0.47* *
0.97* *
The diagonal data (bold) are correlation coefficients between the 2009-2010 and 2010-2011 growing season, and the data below and above diagonal are correlation coefficients between parameters in the 2009-2010 and 2010-2011 growing season, respectively. * * indicate significance at the 0.01 probability level. 对角线(加粗体)数据为两年度间相关系数, 对角线下方数据为2009-2010年度参数间的相关系数, 对角线上方数据为2010-2011年度参数间的相关系数。* * 表示在0.01概率水平相关。
表2 宁麦9号及其衍生系揉混参数的相关系数 Table 2 Correlation coefficients of mixograph parameters in Ningmai 9 and its derivatives
表3 宁麦9号及其衍生系中与揉混参数相关联的分子标记 Table 3 Molecular markers associated with mixograph parameters in Ningmai 9 and its derivatives
标记 Marker
染色体 Chr.
2009-2010
2010-2011
P (× 10-3)
贡献率 R2(%)
方向 Direction
P (× 10-3)
贡献率 R2(%)
方向 Direction
和面时间 Mix time
Xwmc594
3A
4.42
10.94
-
2.43
12.33
-
Xwmc525
7A
4.48
10.00
+
Xgwm577
7B
4.59
10.90
-
峰值高度 Peak height
Xwmc154
2B
7.03
6.68
-
Xgwm617
6A
2.12
8.27
-
Xgwm539
2D
9.86
5.71
-
峰值宽度 Peak width
Xgwm299
3B
2.66
7.41
-
4.83
6.46
-
Xwmc749
6D
6.33
6.07
+
8 min带宽Eight-minute width
Xcfd72
1D
3.12
7.74
-
Xwmc11
3A
3.12
7.74
-
3.19
7.52
-
Xbarc320
5D
8.03
6.18
9.34
5.80
-
Xbarc110
5D
3.06
7.82
-
8.03
6.18
-
Xgwm577
7B
9.04
6.18
-
0.43
11.24
-
Xgwm349
2D
6.98
6.30
+
Xwmc594
3A
4.75
6.89
-
+ and - indicate positive and negative effect of the marker on the corresponding trait, respectively. +和-分别表示标记对其对应性状有正向和负向效应。
表3 宁麦9号及其衍生系中与揉混参数相关联的分子标记 Table 3 Molecular markers associated with mixograph parameters in Ningmai 9 and its derivatives
表4 Table 4 表4(Table 4)
表4 揉混参数关联位点的表型均值及宁麦9号与非宁麦9号基因型间的t-检验结果 Table 4 Means of mixograph parameters on associated loci and t-test between Ningmai 9 and non-Ningmai 9 genotypes
揉混参数 Mixograph parameter
标记 Marker
宁麦9号基因型 Ningmai 9 genotype
非宁麦9号基因型 Non-Ningmai 9 genotype
t值 t-value
P值 P-value
和面时间 Mix time (min)
Xwmc594
1.30
1.58
2.794
0.006
峰值高度 Peak height (%)
Xgwm299
40.78
46.62
2.751
0.006
峰值宽度 Peak width (%)
Xwmc11
27.37
37.28
2.231
0.027
8 min带宽 Eight-minute width (%)
Xbarc320
26.69
29.30
2.004
0.047
Xbarc110
24.58
27.53
5.199
0.000
Xgwm577
26.62
30.46
2.859
0.005
表4 揉混参数关联位点的表型均值及宁麦9号与非宁麦9号基因型间的t-检验结果 Table 4 Means of mixograph parameters on associated loci and t-test between Ningmai 9 and non-Ningmai 9 genotypes
表5 Table 5 表5(Table 5)
表5 揉混参数关联位点在宁麦9号衍生世代中的分布频率 Table 5 Frequencies of markers associated with mixograph parameters in Ningmai 9 derived generations (%)
世代 Generation
Xwmc594
Xgwm299
Xwmc11
Xbarc320
Xbarc110
Xgwm577
衍生一代 First generation
52.63
79.49
66.67
65.79
57.89
88.57
衍生二代 Second generation
97.40
98.72
66.67
69.23
43.59
72.37
全部世代 Combined
82.61
92.31
66.67
68.10
48.28
77.48
表5 揉混参数关联位点在宁麦9号衍生世代中的分布频率 Table 5 Frequencies of markers associated with mixograph parameters in Ningmai 9 derived generations (%)
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