关键词:马铃薯; 薯形; 标记开发; 分子标记辅助育种 Development and Verification of a CAPS Marker Linked to Tuber Shape Gene in Potato ZHU Wen-Wen1, XU Jian-Fei1, LI Guang-Cun1, DUAN Shao-Guang1, LIU Jie1, BIAN Chun-Song1, PANG Wan-Fu1, Walter De Jong2, JIN Li-Ping1,* 1 Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crops, Ministry of Agriculture, Beijing 100081, China
2 Department of Plant Breeding and Genetics, Cornell University, Ithaca 14853, NY, USA
AbstractTuber shape is one of the most important traits for potato cultivars especially for the processing varieties. Previous study shows that a single gene Roon chromosome 10 controls most variation for round versus long tuber shape. In this study, based on the potato genome sequence information and bulked segregation analysis strategy, we developed a CAPS marker 1137-CAPSVI using the diploid potato genotypes 320-02, 10618-01 and their 213 F1 tuber shape segregated population. This marker was verified in 53 tetraploid breeding lines with diverse tuber shapes. The results showed that the consistency rate between the marker and tuber shape was 83.02% and the accuracy of the CAPS marker 1137-CAPSVI for selecting round tuber shape was up to 91.42%, and the further Pearson correlation analysis result showed that the marker was significantly correlated with tuber shape. The CAPS marker provides a basis for molecular marker-assisted selection in potato breeding especially for processing variety.
Keyword:Potato; Tuber shape; Marker development; Molecular marker-assisted breeding Show Figures Show Figures
图1 部分引物在亲本320-02和10618-01中的扩增产物电泳图图中每对引物前面泳道对应320-02, 后面泳道对应10618-01。Fig. 1 PCR products electrophoresis analysis of partial primers in parents 320-02 and 10618-01For each primer in the figure, the front lane represents 320-02, and the later lane represents 10618-01.M: DNA ladder; 7-45: 1137-F45R45; 7-46: 1137-F46R46; 7-47: 1137-F47R47; 7-48: 1137-F48R48; 7-49: 1137-F49R49; 7-50: 1137-F50R50; 7-51: 1137-F51R51; 7-52: 1137-F52R52; 7-53: 1137-F54R54; 7-54: 1137-F54R54; 7-55: 1137-F55R55; 7-56: 1137-F56R56.
图4 1137-CAPSVI标记在53份四倍体材料中的PCR产物酶切分析M: DNA marker; 1~53分别对应表1各材料编号。Fig. 4 Restriction pattern of 1137-CAPSVI PCR products on 53 tetraploid clonesM: DNA marker; 1-53 corresponds to the material codes in Table 1, respectively.
表 3 53份四倍体材料表型鉴定和标记检测结果 Table 3 Phenotype and marker test results of 53 tetraploid clones
标记鉴定 Marker test
表型鉴定 Phenotypic test
基因型数 No. of genotypes
合计 Total
百分比 Percentage (%)
标记阳性 Marker positive
圆薯形 Round tuber
32
44
83.02
标记阴性 Marker negative
长薯形 Long tuber
12
标记阳性 Marker positive
长薯形 Long tuber
6
9
16.98
标记阴性 Marker negative
圆薯形 Round tuber
3
表 3 53份四倍体材料表型鉴定和标记检测结果 Table 3 Phenotype and marker test results of 53 tetraploid clones
4 结论开发了一个可用于四倍体马铃薯薯形辅助选择的分子标记1137-CAPSVI, 该标记对圆薯形材料的选择准确率可达91.42%, 这对利用标记辅助选择加速马铃薯加工品种的选育和进一步探明薯形的遗传机制具有重要意义。 The authors have declared that no competing interests exist.
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