关键词:棉花; 苗期; 耐盐性鉴定; 最大光化学效率; 耐盐指数 An Efficient Approach to Identify Salt Tolerance of Upland Cotton at Seedling Stage PENG Zhen, HE Shou-Pu, SUN Jun-Ling, XU Fei-Fei, JIA Yin-Hua, PAN Zhao-E, WANG Li-Ru, DU Xiong-Ming* Institute of Cotton Research, Chinese Academy of Agricultural Sciences / State Key Laboratory of Cotton Biology, Anyang 455000, China Fund: AbstractThree-leaf cotton seedlings of two salt-tolerant varieties and two salt-sensitive varieties were treated by water and 4% (40 g L-1) NaCl solution, respectively. A total of 13 parameters related to salt tolerance including salt injury index (SII), shoot fresh weight (SRW), root fresh weight (RFW), leaf relative water content (RWC), chlorophyll fluorescence parameters (Fo,Fm,Fv/Fm), relative conductivity (RC), manlondialdehyde (MDA) content and the activity of antioxidant enzymes were monitored after 72 h of treatment. Comprehensive assessment of salinity tolerance based on grey relation clustering method, principal component analysis and stepwise regression analysis indicated that the maximum quantum yield of PSII was the most significant correlated indices with salt tolerance in upland cotton, which could be used as a single parameter to assess salt tolerance based on the equation:y= 1.943x- 0.882 (whereyis the salt tolerance index,xis the related value of maximum efficiency of photosystem II). The other two salt-tolerant varieties and two salt-sensitive varieties were used to rate the classification of salt tolerance. Salt tolerance index (y) of 23 varieties with known salt tolerance were calculated to validate the accuracy of the equation, the result was consistent with field investigation. In this study, the maximum efficiency of photosystem II was used as the main index to validate the salt tolerance in cotton, together with the construction of salt tolerance index equation and salt tolerance rating, which could greatly improve the efficiency of salinity tolerance evaluation for massive germplasm in future.
Keyword:Upland cotton; Seedling stage; Salt tolerance evaluation; Maximum quantum yield of PSII; Index of salinity tolerance Show Figures Show Figures
表6 C1、C2指标的主成分特征向量、主成分特征值、贡献率及累计贡献率 Table 6 Power vector (PV), eigenvalues (E), contribution rate (CR), and cumulative contribution rate (CCR) of principal components in C1 and C2 indices
分类 Classify
指标 Index
特征向量 PV
特征值 E
贡献率 CR (%)
累积贡献率 CCR (%)
C1
SII
0.167
5.905
98.422
98.422
RWC
0.169
Fo
0.165
Fm
0.168
Fv/ Fm
0.169
Fv/ Fo
0.169
C2
SFW
0.149
5.954
85.056
85.056
RFW
0.167
RC
0.148
MDA
0.139
POD
0.150
SOD
0.167
APX
0.161
表6 C1、C2指标的主成分特征向量、主成分特征值、贡献率及累计贡献率 Table 6 Power vector (PV), eigenvalues (E), contribution rate (CR), and cumulative contribution rate (CCR) of principal components in C1 and C2 indices
表7 Table 7 表7(Table 7)
表7 4个棉花品种主成分分析得分及综合耐盐 D值与传统PCA分析方法的比较 Table 7 Comparison of score calculated by principal components and by traditional PCA analysis in four cotton varieties
早熟长绒7号 Zaoshuchangrong 7
中棉所35 CRI35
宿棉9108 Sumian 9108
南丹巴地大花 Nandanbadidahua
C1类指标得分 Score of class C1 index ( F1)
0.915
0.962
0.078
0.011
C2类指标得分 Score of class C2 index ( F2)
0.919
0.567
0.133
0.097
PCA主成分得分Y1 PCA scores Y1
2.075
1.821
0.216
0.504
PCA主成分得分Y2 PCA scores Y2
0.387
-0.407
0.085
0.026
传统PCA综合得分 Traditional PCA score
1.854
1.529
0.199
0.441
田间特性 Characteristics of field
耐盐Tolerant
耐盐Tolerant
盐敏感Sensitive
盐敏感Sensitive
综合耐盐 D值 Consolidated D values
0.917
0.798
0.101
0.047
D值耐盐排序 Sort by salt D values
1
2
3
4
PCA耐盐排序 Sort by salt PCA values
1
2
4
3
表7 4个棉花品种主成分分析得分及综合耐盐 D值与传统PCA分析方法的比较 Table 7 Comparison of score calculated by principal components and by traditional PCA analysis in four cotton varieties
4 结论在可控条件下以沙土为基质, 4% NaCl溶液浇灌棉花幼苗, 持续处理72 h, 利用统计学方法, 从测定的13个棉花耐盐相关指标中筛选出最大光化学效率( Fv/ Fm)作为最优指标, 建立回归方程, 依据耐盐指数( y)划分出耐盐性等级, 建立了一套陆地棉苗期耐盐性的鉴定方法。经验证, 该方法可以对陆地棉品种资源苗期耐盐性进行大规模的鉴定。 致谢: 感谢长江大学付鹏同学在温室鉴定试验中给予的大力协助。 The authors have declared that no competing interests exist. 作者已声明无竞争性利益关系。The authors have declared that no competing interests exist.
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