Abstract:A self-developed pilot denitrification biological filter was used to study the relationship between biofilm mass and nitrogen removal efficiency, dehydrogenase activity in stable state when treating the secondary effluent from a conventional activated sludge process. The results showed that SS could be divided into three regions: region 1(232.5~1 246.6 mg·L?1), region 2(1 246.6~2 542.7 mg·L?1), region 3(2 542.7~3 523.9 mg·L?1) according to VSS/SS ratio equal to 0.78, higher than 0.78 or less than 0.78. In Region 2, the maximum removal rates of ${\rm{NO}}_3^ - $-N and TN were obtained, and they were 95.0% and 85.7%, respectively. At the same time, the maximum total dehydrogenase activity(TDHA) was also obtained with a value of 112.5 g. Biological membrane dehydrogenase activity per unit mass (DHA) was negatively correlated with SS and VSS, with the R2 of 0.822 and 0.876, respectively. TDHA increased with the increase of SS, and decreased until VSS/SS began to decrease. DHA could better reflect the microbial activity at a micro-level, while TDHA could reflect the biological activity of the whole reactor at a macro-level, which provides theoretical basis for the denitrification biological filter operation. Key words:dehydrogenase activity/ biofilm mass/ nitrogen removal efficiency/ denitrification biological filter.
图1实验装置示意图 Figure1.Schematic diagram of the experimental setup
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1.Suzhou Industrial Park Qingyuan & Hong Kong China Water Co. Ltd., Suzhou 215021, China 2.Research Institute for Water and Waste Management at RWTH Aachen University, Aachen 52072, Germany Received Date: 2019-07-24 Accepted Date: 2019-11-01 Available Online: 2020-05-12 Keywords:dehydrogenase activity/ biofilm mass/ nitrogen removal efficiency/ denitrification biological filter Abstract:A self-developed pilot denitrification biological filter was used to study the relationship between biofilm mass and nitrogen removal efficiency, dehydrogenase activity in stable state when treating the secondary effluent from a conventional activated sludge process. The results showed that SS could be divided into three regions: region 1(232.5~1 246.6 mg·L?1), region 2(1 246.6~2 542.7 mg·L?1), region 3(2 542.7~3 523.9 mg·L?1) according to VSS/SS ratio equal to 0.78, higher than 0.78 or less than 0.78. In Region 2, the maximum removal rates of ${\rm{NO}}_3^ - $-N and TN were obtained, and they were 95.0% and 85.7%, respectively. At the same time, the maximum total dehydrogenase activity(TDHA) was also obtained with a value of 112.5 g. Biological membrane dehydrogenase activity per unit mass (DHA) was negatively correlated with SS and VSS, with the R2 of 0.822 and 0.876, respectively. TDHA increased with the increase of SS, and decreased until VSS/SS began to decrease. DHA could better reflect the microbial activity at a micro-level, while TDHA could reflect the biological activity of the whole reactor at a macro-level, which provides theoretical basis for the denitrification biological filter operation.