Nitrogen and phosphorus removal performance of microelectrolysis coupled with solid denitrification and its microbial community analysis
TANG Jing1,,, YANG Yufei1, CHEN Jinnan2 1.School of Municipal and Environmental Engineering, Shenyang Jianzhu University, Shenyang 110168, China 2.Department of Municipal and Environmental Engineering, Shenyang Urban Construction University, Shenyang 110167, China
Abstract:In order to improve the removal efficiency of nitrogen and phosphorus in sewage with low carbon-nitrogen ratio, the effects of HRT, DO and pH on nitrogen and phosphorus removal were investigated in a coupled system of iron-carbon microelectrolysis and solid denitrification. The microbial community structure in the iron carbon particles (FC), solid carbon source particles (CC) and suspended sludge (SS) were analyzed. The results showed that when the influent C/N ratio was 1.5, the optimal operating parameters of the coupling system were HRT=4 h, DO=2.0 mg·L?1, pH=7.0, and the removal rates of ${\rm{NH}}_4^ + $-N, ${\rm{NO}}_3^ - $-N, TN, TP were 95.63%, 93.48%, 94.72% and 99.10%, respectively. High-throughput sequencing analysis demonstrated that at the phylum level, the dominant bacteria in the three samples (FC, CC, SS) were Proteobacteria, Actinobacteria and Bacteroidetes, respectively. Proteobacteria with denitrifying and denitrifying functions in FC, CC, SS were accounted for 72.66%, 67.43% and 68.66% of the total sample, respectively. At the class level, the relative abundance of Alphaproteobacteria in SS was significantly higher than that in FC and CC, while Gammaproteobacteria in FC was significantly higher than that in CC and SS. Especially, the relative abundance of Gemmatimonadetes in CC was significantly higher than that of FC and SS, indicating that biological of phosphorus removal mainly occurred in CC. At the genus level, the relative abundances of Gemmobacter in FC, CC and SS were 25.50%, 23.64% and 32.53%, respectively, which played an important role in denitrification. This study would be helpful to improve the understanding of microbial ecology in the coupled system of microelectrolysis-autotrophic/autotrophic denitrifying and phosphorus removal. Key words:iron carbon microelectrolysis/ solid denitrification/ nitrogen and phosphorus removal/ low carbon to nitrogen ratio/ microbial community.
图1铁碳微电解填料对脱氮除磷效果的影响 Figure1.Effect of iron-carbon microelectrolysis filler on nitrogen and phosphorus removal
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1.School of Municipal and Environmental Engineering, Shenyang Jianzhu University, Shenyang 110168, China 2.Department of Municipal and Environmental Engineering, Shenyang Urban Construction University, Shenyang 110167, China Received Date: 2019-07-09 Accepted Date: 2019-10-15 Available Online: 2020-05-12 Keywords:iron carbon microelectrolysis/ solid denitrification/ nitrogen and phosphorus removal/ low carbon to nitrogen ratio/ microbial community Abstract:In order to improve the removal efficiency of nitrogen and phosphorus in sewage with low carbon-nitrogen ratio, the effects of HRT, DO and pH on nitrogen and phosphorus removal were investigated in a coupled system of iron-carbon microelectrolysis and solid denitrification. The microbial community structure in the iron carbon particles (FC), solid carbon source particles (CC) and suspended sludge (SS) were analyzed. The results showed that when the influent C/N ratio was 1.5, the optimal operating parameters of the coupling system were HRT=4 h, DO=2.0 mg·L?1, pH=7.0, and the removal rates of ${\rm{NH}}_4^ + $-N, ${\rm{NO}}_3^ - $-N, TN, TP were 95.63%, 93.48%, 94.72% and 99.10%, respectively. High-throughput sequencing analysis demonstrated that at the phylum level, the dominant bacteria in the three samples (FC, CC, SS) were Proteobacteria, Actinobacteria and Bacteroidetes, respectively. Proteobacteria with denitrifying and denitrifying functions in FC, CC, SS were accounted for 72.66%, 67.43% and 68.66% of the total sample, respectively. At the class level, the relative abundance of Alphaproteobacteria in SS was significantly higher than that in FC and CC, while Gammaproteobacteria in FC was significantly higher than that in CC and SS. Especially, the relative abundance of Gemmatimonadetes in CC was significantly higher than that of FC and SS, indicating that biological of phosphorus removal mainly occurred in CC. At the genus level, the relative abundances of Gemmobacter in FC, CC and SS were 25.50%, 23.64% and 32.53%, respectively, which played an important role in denitrification. This study would be helpful to improve the understanding of microbial ecology in the coupled system of microelectrolysis-autotrophic/autotrophic denitrifying and phosphorus removal.
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