程艳¹,
刘杰^1,2,,,
蒋萍萍^1,2,
俞果^1,2,
蒋旭升¹,
丁志凡¹,
雷玲¹,
张冰¹
1. 桂林理工大学, 广西环境污染控制理论与技术重点实验室, 桂林 541004;
2. 自然资源部南方石山地区矿山地质环境修复工程技术创新中心, 桂林 541004

作者简介: 程艳(1996-),女,硕士研究生,研究方向为土壤重金属污染植物修复,E-mail:2672469167@qq.com.

通讯作者: 刘杰,liujie@glut.edu.cn ;

基金项目: 国家自然科学基金资助项目（41867022）；广西高等学校高水平创新团队及卓越****计划资助项目（桂财教函[2018]319号）


中图分类号: X171.5

Mitigation Effect of Mn on Cd Toxicity in a Hyperaccumulator Celosia argentea Linn.

Cheng Yan¹,
Liu Jie^1,2,,,
Jiang Pingping^1,2,
Yu Guo^1,2,
Jiang Xusheng¹,
Ding Zhifan¹,
Lei Ling¹,
Zhang Bing¹
1. Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin 541004, China;
2. Technical Innovation Center of Mine Geological Environmental Restoration Engineering in Southern Karst Area, Ministry of Natural Resources, Guilin 541004, China

Corresponding author: Liu Jie,liujie@glut.edu.cn ;

CLC number: X171.5

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摘要:为了探究Cd胁迫下青葙中Mn的生理作用，通过水培实验，研究在Cd暴露下（0、5和25 μmol·L^-1）施加Mn（5、100和1 000 μmol·L^-1）对青葙的干重、叶绿素含量、Cd含量和青葙根部不同区域实时Cd²⁺流速的影响。结果表明，提高Mn的供应水平可缓解Cd对青葙生长的抑制效应。在Cd浓度为5 μmol·L^-1，施加1 000 μmol·L^-1的Mn时，青葙的根、茎、叶干重和总干重分别增加了26.8%、11.8%、22.7%和21.19%。当Cd处理浓度为25 μmol·L^-1时，1 000 μmol·L^-1的Mn显著增加了青葙的叶绿素a和总叶绿素的含量（P<0.05）。相反地，Mn的添加显著减少了叶片中的丙二醛含量（P<0.05）。在Cd处理为5 μmol·L^-1和25 μmol·L^-1时，施加Mn使丙二醛含量降低了30%。这表明，Mn浓度的增加能够减少Cd对青葙的脂质过氧化伤害，保护叶绿素。Mn对青葙Cd积累的影响并不是严格的拮抗效应。在低Cd浓度（5 μmol·L^-1）处理组中，Mn的添加对青葙Cd累积无显著影响（P>0.05）。在高浓度处理时（25 μmol·L^-1 Cd），1 000 μmol·L^-1的Mn显著降低了青葙根、茎和叶的Cd含量（P<0.05），较施加5 μmol·L^-1 Mn处理分别降低了41.4%、41.5%和23.3%。这可能与Mn²⁺抑制青葙根系对Cd²⁺的吸收有关。非损伤微测技术（NMT）分析结果显示，Mn²⁺的添加显著抑制了根表面Cd²⁺的内流速率。当加入50 μmol·L^-1的Mn时，距根尖200 μm的Cd²⁺的内流速率下降了71.4%。上述结果表明，施加Mn有效地缓解了Cd对青葙的毒性效应。
关键词: Cd/
Mn/
青葙/
缓解效应

Abstract:To explore the mitigation of Cd toxicity to Celosia argentea by Mn, the effects of Mn on the dry weight, chlorophyll content, Cd content and real-time Cd²⁺ flux in different regions of root were studied using a hydroponic experiment with three levels of Cd (0, 5 and 25 μmol·L^-1) and three levels of Mn (5, 100 and 1 000 μmol·L^-1). The results showed that the inhibition of Cd on the growth of C. argentea could be alleviated by increasing the supply of Mn. When the supply concentration of Cd was 5 μmol·L^-1, 1 000 μmol·L^-1 Mn application increased the dry weight of root, stem, leaf, and total dry weight of C. argentea by 26.8%, 11.8%, 22.7% and 21.19%, respectively. When Cd concentration was 25 μmol·L^-1, the contents of chlorophyll a and total chlorophyll in C. argentea were significantly increased by 1 000 μmol·L^-1 Mn (P<0.05). On the contrary, the content of malondialdehyde (MDA) in leaves was significantly decreased by adding Mn (P<0.05). At 5 μmol·L^-1 and 25 μmol·L^-1 Cd treatment, Mn application reduced MDA content by 30%. This finding indicated that elevation of Mn supply can reduce the lipid peroxidation injury of Cd to C. argentea and protect chlorophyll. The effect of Mn on Cd accumulation in C. argentea was not full antagonism. At 5 μmol·L^-1 Cd treatment, the addition of Mn had no significant effect on Cd accumulation (P>0.05). At high concentration Cd treatment (25 μmol·L^-1), application of 1 000 μmol·L^-1 Mn significantly decreased the Cd concentrations in roots, stems and leaves (P<0.05), which were 41.4%, 41.5% and 23.3% lower than those with 5 μmol·L^-1 Mn treatment, respectively. This may be related to the inhibition of Cd²⁺ uptake by C. argentea roots by Mn²⁺. Non-invasive micro-test analysis (NMT) showed that the addition of Mn²⁺ significantly inhibited the Cd²⁺ influxes at the root surface. The addition of 50 μmol·L^-1 Mn²⁺ decreased the rates of Cd²⁺ flux by 71.4% at 200 μm from root tip. The above results showed that the toxic effect of Cd on C. argentea was effectively alleviated by applying Mn.
Key words:Cd/
Mn/
Celosia argentea/
mitigation.

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