齐澈力木格,
尹晓宇,
李嘉伟,
董文静,
陈浩,
王欢,
楚文庆,
杨景峰,
于建华^,,
董武^,
内蒙古民族大学动物科学技术学院, 内蒙古自治区毒物监控及毒理学重点实验室, 通辽 028000

作者简介: 齐澈力木格(1990-),女,硕士研究生,研究方向为毒理学,E-mail:qichelimuge@163.com.

通讯作者: 于建华,yihyjh926@163.com ; 董武,dongwu@imun.edu.cn

基金项目: 国家自然科学基金资助项目（21567019，81360508）；内蒙古民族大学特色交叉学科群建设项目（MDXK008）；内蒙古自治区自然科学基金资助项目（2018MS08033）；内蒙古草原英才2020年度滚动支持（董武）；内蒙古毒物监测及毒理学重点实验室开放项目（MDK2019074，MDK2019076）；内蒙古民族大学研究生科研创新项目（NMDSS1860）；蒙药研发国家地方联合工程研究中心开放基金资助项目（MDK2019051）


中图分类号: X171.5

Parental Tetracycline Hydrochloride Exposure and Resultant Offspring Cartilage Toxicity

Qi Chelimuge,
Yin Xiaoyu,
Li Jiawei,
Dong Wenjing,
Chen Hao,
Wang Huan,
Chu Wenqing,
Yang Jingfeng,
Yu Jianhua^,,
Dong Wu^,
College of Animal Science and Technology, Inner Mongolia University for Nationalities, Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, Tongliao 028000, China

Corresponding authors: Yu Jianhua,yihyjh926@163.com ; Dong Wu,dongwu@imun.edu.cn

CLC number: X171.5

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摘要:抗生素被广泛应用于兽药和饲料添加剂中，尤其是四环素等广谱抗生素，长期滥用于养殖业中。四环素是目前用量最大、应用范围最广泛的一种抗生素。抗生素在体内蓄积，或者以原型随粪便排入环境中，造成环境污染。实验用斑马鱼为模式动物来评价低浓度四环素对斑马鱼下一代（F1）发育的毒性影响。选取4月龄亲代斑马鱼（F0），分别给予0.1、1和100 μg·L^-1盐酸四环素（tetracycline hydrochloride，TCH）处理30 d后，实施交配后获得F1代斑马鱼胚胎。结果表明，F1代胚胎随母体TCH暴露浓度的升高而孵化率降低、畸形率和死亡率增加。同时斑马鱼幼鱼下颌骨长度、下颌弓长度变长、下颌骨宽度和舌骨角长度缩短。进一步检测与幼鱼骨骼发育相关基因表达，发现TCH抑制了runx1、sox9a、sox10和col2α1a mRNA的表达。以上研究结果表明，TCH的残留和污染可能会影响斑马鱼胚胎的发育，尤其是对胚胎软骨发育有影响。
关键词: 盐酸四环素/
斑马鱼胚胎/
F1代/
毒性/
软骨发育

Abstract:Antibiotics are widely used as veterinary drugs and feed additives, especially for broad-spectrum antibiotics such as tetracycline, which have been abused in the breeding industry. Tetracycline is the antibiotics with the largest dosage and the widest application. Antibiotics accumulate in the body or are excreted into the environment via feces as a prototype, causing environmental pollution. This experiment used zebrafish as a model organism to evaluate the effects of low concentration of tetracycline on the transgenerational (F1) zebrafish. Parent zebrafish (F0, approximantely 4-month-old) were exposed to 0.1, 1 and 100 μg·L^-1 of tetracycline hydrochloride (TCH) for 30 d. The F1 generation zebrafish embryos were obtained. The results showed that the F1 generation embryos had a decreased hatching rate and an increased malformation rate and mortality rate, all of which were concentration-dependent. In addition, the zebrafish juvenile had longer mandible and mandibular arch and more narrow mandible and shorter hyoid bone. Furthermore, transcriptional expression of runx1, sox9a, sox10 and col2α1a (genes related to skeletal development of juvenile fish) were inhibited by exposure to TCH. This study demonstrates that TCH showed a transgenerational effect by affecting the development of zebrafish embryos, especially the development of embryonic cartilage.
Key words:tetracycline hydrochloride/
zebrafish embryo/
F1 generation/
toxicity/
cartilage development.

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