用于单克隆抗体纯化的仿生多肽超大孔PGMA微球制备及其性能

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葛佳^1,2，那向明^1,2，吴学星²，杨维兴³，郝冬霞^2*，马光辉^1,2*

1. 中国科学院大学化学工程学院，北京 1000492. 中国科学院过程工程研究所生化工程国家重点实验室，北京 1001903. 中科森辉微球技术(苏州)有限公司，江苏苏州 215000

收稿日期:2019-03-25修回日期:2019-05-05出版日期:2020-01-22发布日期:2020-01-14
通讯作者:郝冬霞

基金资助:国家自然科学基金项目;国家自然科学基金项目;北京市自然科学基金-北京科学技术研究院联合基金项目

Preparation of biomimetic peptide attached supermacroporous poly(glycidyl methacrylate) microspheres for monoclonal antibody purification and its performance

Jia GE^1,2, Xiangming NA^1,2, Xuexing WU², Weixing YANG³, Dongxia HAO^2*, Guanghui MA^1,2*

1. School of Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, China2. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering,Chinese Academy of Sciences, Beijing 100190, China3. Zhongke Senhui Microsphere Technology (Suzhou) Co., Ltd., Suzhou, Jiangsu 215000, China

Received:2019-03-25Revised:2019-05-05Online:2020-01-22Published:2020-01-14

摘要/Abstract

摘要： 以仿生多肽配基FYEILHC为亲和配基、以葡聚糖修饰的聚甲基丙烯酸缩水甘油酯[Dextran-poly(glycidyl methacrylate), Dextran-PGMA]超大孔微球为基质，制备用于单克隆抗体纯化的仿生多肽超大孔PGMA微球，在环氧氯丙烷中滴加2 mol/LNaOH使其表面衍生出环氧基，在表面修饰FYEILHC；用扫描电镜表征微球表面形貌，用AKTA蛋白纯化系统考察了Dextran-PGMA微球和琼脂糖微球对抗体的动态吸附量随线性流速的变化。结果表明，偶联FYEILHC后Dextran-PGMA微球仍能保持其大孔结构，在923 cm/h线性流速下，其对抗体的动态吸附量仅下降约8%，而琼脂糖微球的动态吸附量则迅速下降25%。表明在较高流速下，抗体在Dextran-PGMA微球上的传质性能较好。吸附?用0.1 mol/L NaOH原位清洗重复40次后，Dextran-PGMA微球对抗体的动态吸附量约为(21?1) mg/mL，表明微球具有良好的化学稳定性；血清中回收的抗体纯度为95.0%，表明仿生多肽亲和介质具有从复杂生物样品中纯化抗体的巨大潜力，可满足高流速、高通量抗体分离纯化需求。

引用本文

葛佳那向明吴学星杨维兴郝冬霞马光辉. 用于单克隆抗体纯化的仿生多肽超大孔PGMA微球制备及其性能[J]. 过程工程学报, 2020, 20(1): 84-90.
Jia GE Xiangming NA Xuexing WU Weixing YANG Dongxia HAO Guanghui MA. Preparation of biomimetic peptide attached supermacroporous poly(glycidyl methacrylate) microspheres for monoclonal antibody purification and its performance[J]. Chin. J. Process Eng., 2020, 20(1): 84-90.

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