一种用高效凝胶渗透色谱测试褐藻胶分子量及分子量分布的方法

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张蕊鑫^1,2，王智慧^1*，丛威¹，惠锋基³，王斌³

1. 中国科学院过程工程研究所生化工程国家重点实验室，北京 1001902. 中国科学院大学化工学院，北京 1000493. 山东洁晶集团股份有限公司，山东日照 276800

收稿日期:2019-04-10修回日期:2019-05-12出版日期:2020-01-22发布日期:2020-01-14
通讯作者:王智慧

Molecular weight and molecular weight distribution of alginate determination by gel permeation chromatography

Ruixin ZHANG^1,2, Zhihui WANG^1*, Wei CONG¹, Fengji HUI³, Bin WANG³

1. State Key Laboratory Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China 2. School of Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, China 3. Shandong Jiejing Group Co., Ltd., Rizhao, Shandong 276800, China

Received:2019-04-10Revised:2019-05-12Online:2020-01-22Published:2020-01-14

摘要/Abstract

摘要： 提出了一种用凝胶色谱与常规检测器检测褐藻胶分子量及其分布的方法。用高效凝胶渗透色谱(GPC)与示差检测器(RID)结合，建立了以普鲁兰标准品为对照的相对重均分子量、相对数均分子量与GPC–MALLS(多角度激光散射)测试的褐藻胶绝对重均分子量、绝对数均分子量间的转换关系，得到了用GPC–RID检测相对分子量计算褐藻胶绝对分子量和多分散系数的方法。结果表明，GPC–RID法经转换公式得到的重均分子量、数均分子量和多分散系数与GPC–MALLS法的相对误差在?12%以内。基于GPC–RID法可较准确地获得褐藻胶的绝对分子量及多分散系数，使用通用检测器可节约仪器投资，降低检测成本。

引用本文

张蕊鑫王智慧丛威惠锋基王斌. 一种用高效凝胶渗透色谱测试褐藻胶分子量及分子量分布的方法[J]. 过程工程学报, 2020, 20(1): 99-107.
Ruixin ZHANG Zhihui WANG Wei CONG Fengji HUI Bin WANG. Molecular weight and molecular weight distribution of alginate determination by gel permeation chromatography[J]. Chin. J. Process Eng., 2020, 20(1): 99-107.

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