Abstract 【Objective】 In order to provide a practical and scientific basis for improvement, restoration and reasonable use of degraded grassland, the effects of different organic fertilizers on soil nutrient status and soil bacterial diversity were revealed in the natural Leymus chinensis steppe degraded grassland in Hulunbeier. 【Method】 The field experiments were designed by single factor randomized block design, and the seven treatments were as follows: the control (ck), vermicompost 15 t·hm-2(a1), 30 t·hm-2(a2), 45 t·hm-2(a3), mushroom residues 15 t·hm-2(b1), 30 t·hm-2(b2), 45 t·hm-2(b3). Combined with the physical and chemical properties of soil, this study used the high-throughput sequencing technology of Miseq to analyze the effects of different organic fertilizer treatments on the diversity of soil bacterial communities, and to explore the environmental drivers of bacterial community changes under different organic fertilizer treatments. 【Result】 The results showed that organic fertilization improved the soil nutrient status and shaped the distinct bacterial communities. Compared with ck, the AP content increased significantly by 37.27% under a3 treatment. AK content under b3 and a3 treatments increased significantly by 62.99% and 40.53% compared with the control, respectively. And aboveground biomass was significantly higher than other treatments under b3 treatment (244.11 g·m-2). Moreover, vermincompost and mushroom residues fertilizers significantly increased the richness of the bacterial community. Compared with ck, the richness index increased significantly under a1 and b2 treatments. At the phylum level, a total of 31 taxa were obtained from 21 samples. Actinobacteria, Proteobacteria, Acidobacteria, Verrucomicrobia and Chloroflexi were the dominant groups, and relative abundances account for the bacterial community 85% or more. Actinomycetes were the most abundant under a2 treatment (36.79%). Proteobacteria was higher under b1 and b3 treatments (23.29% and 22.32%, resptectively). Acidobacteria was the highest under a1 treatment (20.69%). And LEfSe showed that more bacterial taxonomic groups were detected under b3 treatment (17 clades, 1 class, 1 order, 4 families and 11 genera). In addition, AN (P=0.001), AK (P=0.005), and SOM (P=0.006) had extremely significant effects on the composition of bacterial communities in the soil (P<0.01), while TK (P=0.014) had not. The composition of soil bacterial community had a significant effect (P<0.05). It showed that AN, AK, SOM and TK were the main driving factors of bacterial community. 【Conclusion】 Organic fertilizer changed the soil bacterial community structure in Leymus chinensis steppe. Our results indicated vermicompost and mushroom residues at 45 t·hm-2 increased available nutrient content, but also enhanced the biodiversity of soil bacterial communities in the grasslands of Leymus chinensis, which contributed to the sustainable development of grassland agro-ecosystems. Keywords:organic fertilizer;soil nutrient;soil bacterial community; Leymus chinensis steppe
PDF (1366KB)元数据多维度评价相关文章导出EndNote|Ris|Bibtex收藏本文 本文引用格式 商丽荣, 万里强, 李向林. 有机肥对羊草草原土壤细菌群落多样性的影响[J]. 中国农业科学, 2020, 53(13): 2614-2624 doi:10.3864/j.issn.0578-1752.2020.13.010 SHANG LiRong, WAN LiQiang, LI XiangLin. Effects of Organic Fertilizer on Soil Bacterial Community Diversity in Leymus chinensis Steppe[J]. Scientia Acricultura Sinica, 2020, 53(13): 2614-2624 doi:10.3864/j.issn.0578-1752.2020.13.010
Table 1 表1 表1不同有机肥处理的土壤化学性质和地上部生物量 Table 1Chemical properties and aboveground biomass of the soils in different organic fertilization treatments
处理 Treatment
pH
全氮 TN (g·kg-1)
全磷 TP (g·kg-1)
全钾 TK (g·kg-1)
有效氮 AN (mg·kg-1)
有效磷 AP (mg·kg-1)
有效钾 AK (mg·kg-1)
有机质 SOM (g·kg-1)
生物量 DM (g·m-2)
ck
6.28±0.10b
2.51±0.25a
0.66±0.12a
31.85±0.61a
172.84±10.24a
7.43±0.94b
179.42±31.01c
55.48±0.90a
172.8±10.68c
a1
6.31±0.02b
2.52±0.30a
0.68±0.10a
32.64±1.44a
175.90±11.67a
8.89±1.39b
209.19±55.79bc
52.15±6.19a
198.38±14.94bc
a2
6.51±0.11b
2.26±0.12a
0.69±0.21a
31.96±0.84a
167.76±3.12a
8.34±1.29b
195.03±32.10bc
50.32±5.18a
180.91±2.90bc
a3
7.02±0.54a
2.47±0.08a
0.73±0.20a
33.12±0.71a
184.54±15.22a
10.20±0.15a
252.14±4.68ab
55.40±5.49a
214.27±5.26bc
b1
6.21±0.08b
2.56±0.28a
0.74±0.26a
32.18±0.51a
183.70±3.66a
9.06±1.87b
250.67±12.13ab
55.75±1.76a
191.49±1.99ab
b2
6.14±0.12b
2.10±0.07a
0.58±0.04a
31.84±0.56a
169.34±6.80a
8.18±0.48b
220.20±23.32bc
48.31±5.60a
219.78±10.73ab
b3
6.17±0.20b
2.40±0.26a
0.55±0.01a
32.59±0.88a
187.96±5.60a
8.84±0.31b
292.44±20.67a
55.78±4.43a
244.11±13.59a
数据为平均值±标准差(n=3);同列数值后不同小写字母表示差异显著(P<0.05)。ck,无施肥处理,a1、a2、a3分别为蚯蚓粪施肥量15、30、45 t·hm-2,b1、b2、b3分别为菌渣施肥量15、30、45 t·hm-2。下同 Data are means ± standard deviation (n=3); Different small letters mean significant differences (P<0.05). ck, no fertilization treatment, a1, a2, a3 are the amount of vermicompost application 15, 30 and 45 t·hm-2, b1, b2, b3 are the amount of mushroom residues application 15, 30 and 45 t·hm-2. The same as below
利用Illumina Mi Seq平台测序结果显示,在所有土壤样品中,总共获得1 405 452个序列读数。除去短的、低质量、单体、重复和嵌合体之外,保留了955 201个有效序列。基于97%的相似性,在所有样品中获得总共3 481个OTU,划分为31个门,75个纲,153个目和298个科。所将数据抽平一次,每个样品得到35 708条高质量序列(表2)。
Table 2 表2 表2不同有机肥处理下土壤细菌测序及群落α多样性指数 Table 2Illumina MiSeq sequencing results and α-diversity of the bacterial community with different organic fertilizer treatments
Fig. 4Canonical correspondence analysis (RDA) based on the relative abundance of bacterial at genus level and selected soil chemical properties among different organic fertilization treatments
Table 3 表3 表3基于属水平的细菌群落和环境因子之间相关性的P值 Table 3P value based on the correlation between bacterial communities and environmental factors at the genus level (n=3)
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