Effects of Adding Mannan Oligosaccharides to Milk Replacer on the Development of Gastrointestinal Tract of 7-28 Days Old Hu Lambs
ZHENG Chen1, LI FaDi2,3, LI Fei2, ZHOU JuWang1, DUAN PengWei1, LIU HuiHui1, FAN HaiMiao1, ZHU WeiLi1, LIU Ting,11 College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070 2 State Key Laboratory of Grassland Agro-ecosystems, Key laboratory of Grassland Livestock Industry Innovation/Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020 3 Engineering Laboratory of Mutton Sheep Breeding and Reproduction Biotechnology in Gansu Province, Minqin 733300, Gansu
Abstract 【Objective】This study was conducted to investigate the effects of mannan oligosaccharides (MOS) supplementation to milk replacer on the development of gastrointestinal tract of 7-28 day-old Hu lambs. 【Method】 Thirty 7 day-old Hu male lambs were chosen and divided into 2 groups randomly, fifteen lambs in each group and each lamb as a repeat. Lambs were fed milk replacer with or without 0.2 % MOS, respectively. The test lasted for 21 days. Eight lambs were selected from each group randomly and slaughtered at 28 day-old. The weights of the compound stomach and the intestinal tract with and without content, and lengths of the intestinal tract were measured, and the relative quality and length were calculated. While the tissue samples from fundus gland region of the abomasum, the middle part of duodenum, jejunum and ileum were fixed in paraformaldehyde to analyse the histomorphology, and the apoptotic rate of intestinal epithelial cells as well. And the mRNA expression of claudin 1, zonula occludens-1 (ZO-1), and occludin protein of duodenum, jejunum and ileum mucosa were measured.【Result】The results showed that except relative length of jejunum (P=0.040), the relative weights (% body weight, % stomach weight, % intestinal tract weight, and % gastrointestinal tract weight), relative lengths (% intestinal tract length), content of stomach and intestinal tract (% body weight, % stomach content weight, % intestinal tract content weight, and % gastrointestinal tract content weight), the apoptotic rate of intestinal epithelial cells and mRNA expression of claudin 1 protein in intestinal tract of lambs were not affected by MOS (P>0.05). However, MOS elongated the villus height and the muscular thickness, and decreased the villus width of lamb duodenum significantly (P=0.033, P=0.047, P=0.015). MOS also up-regulated the mRNA expression of ZO-1 protein of lamb jejunum significantly (P=0.028). And there was a tendency that MOS elongated villus height, width and crypt depth of ileum, muscular thickness of abomasum, and mRNA expression of occludin protein of ileum (P=0.075, P=0.078, P=0.085, P=0.084, P=0.052).【Conclusion】MOS almost did not affect the relative weights, lengths, and content distribution of gastrointestinal tract of 7-28 days old Hu lambs, but improved the histomorphology of duodenum and ileum, indicating it could maintain barrier function of intestinal tract and benefit to nutrients digestibility. Keywords:lamb;mannan oligosaccharides;milk replacer;gastrointestinal tract;development
PDF (527KB)元数据多维度评价相关文章导出EndNote|Ris|Bibtex收藏本文 本文引用格式 郑琛, 李发弟, 李飞, 周巨旺, 段鹏伟, 刘绘汇, 樊海苗, 朱威力, 刘婷. 代乳粉添加甘露寡糖对7—28日龄湖羊羔羊胃肠道发育的影响[J]. 中国农业科学, 2020, 53(2): 398-408 doi:10.3864/j.issn.0578-1752.2020.02.014 ZHENG Chen, LI FaDi, LI Fei, ZHOU JuWang, DUAN PengWei, LIU HuiHui, FAN HaiMiao, ZHU WeiLi, LIU Ting. Effects of Adding Mannan Oligosaccharides to Milk Replacer on the Development of Gastrointestinal Tract of 7-28 Days Old Hu Lambs[J]. Scientia Acricultura Sinica, 2020, 53(2): 398-408 doi:10.3864/j.issn.0578-1752.2020.02.014
Table 4 表4 表4MOS对羔羊肠道相对长度的影响 Table 4Effects of MOS on relative length of intestinal tract of lambs (% total intestinal tract length)
项目Item
对照组Control
MOS组 MOS
SEM
P值 P value
十二指肠 Duodenum
2.29
2.01
0.09
0.107
空肠Jejunum
81.84a
80.53b
0.33
0.040
回肠 Ileum
0.83
0.95
0.01
0.292
盲肠 Cecum
0.91
1.12
0.06
0.094
结肠 Colon
11.96
12.89
0.29
0.115
直肠 Rectum
2.17
2.49
0.11
0.151
同行数据后所标字母相异表示差异显著(P<0.05),所标字母相同表示差异不显著(P>0.05)。下同 Different letters in the same row means significant difference between the treatments (P<0.05), same letter in the same row means not significant difference between treatments (P>0.05). The same as below
Table 6 表6 表6MOS对羔羊胃肠内容物相对总胃/肠内容物及总胃肠内容物相对质量的影响 Table 6Effects of MOS on relative quality of each part of stomach and intestinal content to total stomach and intestinal tract content, and gastrointestinal tract content of lambs
项目 Item
对照组Control
MOS组 MOS
SEM
P值 P value
胃室内容物 Stomach content
瘤胃内容物 Rumen content
%全胃内容物质量 % the quality of total stomach content
26.96
33.15
2.56
0.239
%全胃肠内容物质量 % the quality of total gastrointestinal tract content
17.30
21.12
1.80
0.306
网胃内容物 Reticulum content
%全胃内容物质量 % the quality of total stomach content
4.99
6.68
1.28
0.532
%全胃肠内容物质量 % the quality of total gastrointestinal tract content
3.50
4.06
0.03
0.742
瓣胃内容物 Omasum content
%全胃内容物质量 % the quality of total stomach content
0.23
0.24
0.03
0.988
%全胃肠内容物质量 % the quality of total gastrointestinal tract content
0.14
0.13
0.01
0.698
皱胃内容物 Abomasum content
%全胃内容物质量 % the quality of total stomach content
68.58
60.09
2.71
0.119
%全胃肠内容物质量 % the quality of total gastrointestinal tract content
43.14
39.30
2.35
0.434
肠道内容物 Intestinal tract content
十二指肠内容物 Duodenum content
%全肠内容物质量 % the quality of total intestinal tract content
7.20
6.23
1.63
0.777
%全胃肠内容物质量 % the quality of total gastrointestinal tract content
1.98
2.22
0.39
0.765
空肠内容物 Jejunum content
%全肠内容物质量 % the quality of total intestinal tract content
39.34
46.21
2.22
0.126
%全胃肠内容物质量 % the quality of total gastrointestinal tract content
14.28
16.95
1.69
0.451
回肠内容物 Ileum content
%全肠内容物质量 % the quality of total intestinal tract content
1.75
1.45
0.31
0.666
%全胃肠内容物质量 % the quality of total gastrointestinal tract content
0.80
0.57
0.20
0.618
盲肠内容物 Cecum content
%全肠内容物质量 % the quality of total intestinal tract content
12.73
9.88
1.35
0.312
%全胃肠内容物质量 % the quality of total gastrointestinal tract content
4.88
3.51
0.70
0.345
结肠内容物 Colon content
%全肠内容物质量 % the quality of total intestinal tract content
33.02
30.57
2.83
0.681
%全胃肠内容物质量 % the quality of total gastrointestinal tract content
12.49
10.25
1.17
0.356
直肠内容物 Rectum content
%全肠内容物质量 % the quality of total intestinal tract content
5.99
7.80
1.05
0.405
%全胃肠内容物质量 % the quality of total gastrointestinal tract content
Table 8 表8 表8MOS对羔羊小肠黏膜紧密连接蛋白mRNA表达量及上皮细胞凋亡的影响 Table 8Effects of MOS on mRNA expression of intercellular tight junction protein of intestinal tract mucosa and apoptotic rate of intestinal epithelial cells of lambs
GUO JP, PAN JZ, LI FD, ZHANG YX, YANG YZ, HAO ZL . Effect of different early weaned day on morphological development of stomach for housed lambs Acta Veterinaria et Zootechnica Sinica, 2018,49(5):971-985. (in Chinese) [本文引用: 1]
MA JN, TUY . Research progress on feeding patterns of different solid and liquid feed level on growth and gastrointestinal tract development in holstein calves Acta Ecologiae Animalis Domastici, 2017,38(5):7-12. (in Chinese) [本文引用: 1]
MA ZY, LIF, LI FD, LIC, WANG WM, TANG DF, LIUT, PAN XY . Effect of early weaning on performance and gastrointestinal tract development of Hu lambs Chinese Journal of Animal Nutrition, 2015,27(5):1385-1393. (in Chinese) [本文引用: 1]
CHAI JM . Effect of weaning age on the growth performance and development of the gastrointestinal tract in lambs [D]. Beijing: Feed Research Institute, 2015. (in Chinese) [本文引用: 1]
WU ZQ . Effect of different milk allowances and different starter on gastrointestinal development of dairy calves [D]. Taian: Shandong Agricultural University, 2016. (in Chinese) [本文引用: 1]
YANG HB . Effects of pellet diets with different concentrate-roughage ratio on growth performance and development of gastrointestinal tract of 3~6 monthly calves [D]. Yangzhou: Yangzhou University, 2015. (in Chinese) [本文引用: 1]
WU ZH . Effects of different forage supplementary patterns on the growth and gastrointestinal development of holstein calves [D]. Taigu: Shanxi Agricultural University, 2014. (in Chinese) [本文引用: 1]
HEJ . Influence of cysteamine and milk replacer proteins on the development of small intestinal mucosa of goats [D]. Nanjing: Nanjing Agricultural University, 2005. (in Chinese) [本文引用: 1]
BARTLETT KS, MCKEITH FK, VANDEHAAR MJ, DAHL GE, DRACKLEY JK . Growth and body composition of dairy calves fed milk replacers containing different amounts of protein at two feeding rates Journal of Animal Science, 2006,84:1454-1467. [本文引用: 1]
GEIGER AJ, PARSONS C LM, JAMES RE, AKERS RM . Growth, intake, and health of Holstein heifer calves fed an enhanced preweaning diet with or without postweaning exogenous estrogen Journal of Dairy Science, 2016,99:3995-4004. [本文引用: 1]
SOBERONF, VAN AMBURGH ME . Effects of preweaning nutrient intake in the developing mammary parenchymal tissue Journal of Dairy Science, 2017,100:4996-5004. [本文引用: 1]
ADHIKARIP, Kim WK . Overview of prebiotics and probiotics: focus on performance, gut health and immunity - a review Annals of Animal Science, 2017,17:949-966. [本文引用: 1]
HALASV, NOCHTAI . Mannan oligosaccharides in nursery pig nutrition and their potential mode of action Animals, 2012,2:261-274. [本文引用: 1]
WESTLANDA, MARTINR, WHITER, MARTIN JH . Mannan oligosaccharide prepartum supplementation: effects on dairy cow colostrum quality and quantity Animal, 2017,11:1779-1782. [本文引用: 1]
FERNANDEZF, HINTONM, VAN GILSB . Dietary mannan- oligosaccharides and their effect on chicken caecal microflora in relation to Salmonella Enteritidis colonization Avian Pathology, 2002,31:49-58. [本文引用: 1]
NAJDEGERAMI EH, TOKMACHIA, BAKHSHIF . Evaluating the effects of dietary prebiotic mixture of mannan oligosaccharide and poly-β-hydroxybutyrate on the growth performance, immunity, and survival of rainbow trout,Oncorhynchus mykiss(Walbaum 1792), fingerlings Journal of the World Aquaculture Society, 2017,48(3):415-425. [本文引用: 2]
GIANNENASI, DOUKASD, KARAMOUTSIOSA, TZORAA, BONOSE, SKOUFOSI, TSINASA, CHRISTAKIE, TONTISD, FLOROU-PANERIP . Effects of Enterococcus faecium, mannan oligosaccharide, benzoic acid and their mixture on growth performance, intestinal microbiota, intestinal morphology and blood lymphocyte subpopulations of fattening pigs. Animal Feed Science and Technology, 2016,220:159-167. [本文引用: 1]
BOZKURTM, BINTA?E, KIRKANS, AK?ITH, Kü?üKYILMAZK, ERBA?G, ?ABUKM, AK?ITD, PARMU, EGEG, KO?ERB, SEYREKK, TüZüN AE . Comparative evaluation of dietary supplementation with mannan oligosaccharide and oregano essential oil in forced molted and fully fed laying hens between 82 and 106 weeks of age Poultry Science, 2016,95:2576-2591. [本文引用: 1]
ATTIA YA, ABD AL-HAMID AE, IBRAHIM MS, AL-HARTHI MA, BOVERAF, ELNAGGAR AS . Productive performance, biochemical and hematological traits of broiler chickens supplemented with propolis, bee pollen, and mannan oligosaccharides continuously or intermittently Livestock Science, 2014,164:87-95. [本文引用: 1]
TORRECILLASS, CABALLERO MJ, MONTEROD, SWEETMANJ, IZQUIERDOM . Combined effects of dietary mannan oligosaccharides and total fish oil substitution by soybean oil on European sea bass (Dicentrarchus labrax) juvenile diets Aquaculture Nutrition, 2016,22(5):1079-1090. [本文引用: 1]
WEN RZ . Effects of mannose-oligosaccharide on intestinal morphology and bacterial community in gastrointestinal tract of broiler chickens [D]. Nanjing: Nanjing Agricultural University, 2010. (in Chinese) [本文引用: 2]
HUANG JW, LIN YC, FENG DY, ZHENG CT, DING FY . Effect of natto and MOS on intestinal pH, colonic microflora population and intestinal membrane shape of early weaning piglet Acta Veterinaria et Zootechnica Sinica, 2005,36(10):1021-1027. (in Chinese) [本文引用: 2]
MOUR?O JL, PINHEIROV, ALVESA, GUEDES CM, PINTOL, SAAVEDRA MJ, SPRINGP, KOCHERA . Effect of mannan oligosaccharides on the performance, intestinal morphology and cecal fermentation of fattening rabbits Animal Feed Science and Technology, 2006,126:107-120. [本文引用: 1]
DIAZ TG, BRANCO AF, JACOVACI FA, JOBIM CC, BOLSON DC, DANIEL J LP . Inclusion of live yeast and mannan-oligosaccharides in high grain-based diets for sheep: Ruminal parameters, inflammatory response and rumen morphology Plos One, 2018,13:e0193313. [本文引用: 1]
ZHENGC, LI FD, HAO ZL, LIUT . Effects of adding mannan oligosaccharides on digestibility and metabolism of nutrients, ruminal fermentation parameters, immunity, and antioxidant capacity of sheep Journal of Animal Science, 2018,96:284-292. [本文引用: 1]
DEMIRELG, TURANN, TANORA, KOCABAGLIN, ALPM, HASOKSUZM, YILMAZH . Effects of dietary mannanoligosaccharide on performance, some blood parameters, IgG levels and antibody response of lambs to parenterally administered E. coli O157: H7. Archives of Animal Nutrition, 2007,61(2):126-134. [本文引用: 1]
MA ZH. Animal anatomy, histology and embryology .3rd edition. Beijing:China Agriculture Press, 2001. (in Chinese) [本文引用: 1]
LIU JH, XU TT, LIU YJ, ZHU WY, MAO SY . A high-grain diet causes massive disruption of ruminal epithelial tight junctions in goats American Journal of Physiology-Regulatory Integrative and Comparative Physiology, 2013,305:R232-R241. [本文引用: 1]
RENW . Effects of different amylose/amylopectin ratio on the development of gastrointestinal tract and mRNA expression of related genes in fattening lamb [D]. Daqing: Heilongjiang Bayi Agricultural University, 2014. (in Chinese) [本文引用: 2]
KOU ZY, LI QP, MOF, ZHANG XM . Studies on development of digestive organs in calves Proceedings of 6 th National Congress & 8th Symposium of Animal Nutrition Branch of China Animal Husbandry and Veterinary Society (Part Two) , 2000: 533-536. (in Chinese) [本文引用: 1]
ZHENGC . Effects of adding mannan oligosaccharides on digestibility and metabolism of nutrients, ruminal parameters, digesta passage and immune of sheep Lanzhou: Gansu Agricultural University, 2012. (in Chinese) [本文引用: 3]
ZHOUY, DIAO QY, TUY, YUNQ . Effects of yeast β-glucan on gastrointestinal development in early-weaning calves Chinese Journal of Animal Nutrition, 2009,21(6):846-852. (in Chinese) [本文引用: 2]
YAN XG . The effect of yeast culture and pellet concentrate on the growing development of Holstein calves [D]. Changchun: Jilin Agricultural University, 2005. (in Chinese) [本文引用: 1]
WANG CL . Developmental changes of digestive system in grazing sheep from birth to 56d [D]. Lanzhou: Gansu Agricultural University, 2009. (in Chinese) [本文引用: 1]
ZHANGX, WUX, CHENW, ZHANGY, JIANGY, MENGQ, ZHOUZ . Growth performance and development of internal organ, and gastrointestinal tract of calf supplementation with calcium propionate at various stages of growth period PLoS One, 2017a,12:e0179940. [本文引用: 1]
JIN YM, JIANGC, ZHANG XQ, SHI LF, WANG MZ . Effect of dietary Urtica cannabina on the growth performance, apparent digestibility, rumen fermentation and gastrointestinal morphology of growing lambs. Animal Feed Science and Technology, 2018,243:1-9. [本文引用: 1]
YANG QM . The study on digestive enzymes and growth and development of tissues and organs of piglet [D]. Beijing: China Agricultural University, 1999. (in Chinese) [本文引用: 1]
BAKARE AG, CHIMONYOM . Relationship between feed characteristics and histomorphometry of small intestines of growing pigs South African Journal of Animal Science, 2017,47:7-14. [本文引用: 1]
YAO LQ, SA RN, TONG JM, HUO QG . Effect of apramycin on intestinal flora and intestinal morphology of piglets Acta Veterinaria et Zootechnica Sinica, 2003,34(3):250-257. (in Chinese) [本文引用: 1]
SONG EL, CHEN YX, WANG ZX, CHAO GZ, WAN FC, WU NK . Developmental changes on the lengths of different intestinal segments and the morphological structure of small intestinal mucosa in Limisin-crossbred calf Progress in Veterinary Medicine, 2006,27(5):66-70. (in Chinese) [本文引用: 1]
MCLEOD JS, CHURCH JT, YERRAMILLIP, COUGHLIN MA, PERKINS EM, RABAHR, BARTLETT RH, ROJAS-PENAA, GREENSON JK . Gastrointestinal mucosal development and injury in premature lambs supported by the artificial placenta Journal of Pediatric Surgery, 2018,53:1240-1245. [本文引用: 1]
FORSTERC . Tight junctions and the modulation of barrier function in disease Histochemistry and Cell Biology, 2008,130(1):55-70. [本文引用: 1]
PUTHENEDAMM, WILLIAMS PH, LAKSHMI BS, BALAKRISHNANA . Modulation of tight junction barrier function by outer membrane proteins of enteropathogenic Escherichia coli: Role of F-actin and junctional adhesion molecule-1. Cell Biology International, 2007,31(8):836-844. [本文引用: 1]
YANGJ, ZHANG ZW, QIN HL . Protective role of Lactobacillus plantarun in regulating intestinal epithelial cells response to pathogenic bacteria World Chinese Journal of Digestology, 2008,16(30):3394-3399. (in Chinese) [本文引用: 1]
WANG YX . Effect of IUGR on the growth and the intestinal development in postnatal pigs and the nutrition regulation by L-arginine and soya lecithine [D]. Nanjing: Nanjing Agricultural University, 2011. (in Chinese) [本文引用: 1]
SHANG QQ, LI YL, SHI YY, FU AK, LI WF, YU DY . Research progress on probiotics for the immune function of intestinal epithelial cells in animal Chinese Journal of Animal Science, 2014,50(13):87-90. (in Chinese) [本文引用: 1]
YANF, POLK DB . Probiotic bacterium prevents cytokine-induced apoptosis in intestinal epithelial cells The Journal of Biological Chemistry, 2002,277(52):50959-50965. [本文引用: 1]