关键词:花生品种; 指纹图谱; SSR标记; 群体结构 DNA Fingerprinting of Peanut ( Arachis hypogaea L.) Varieties Released in Henan Province SUN Zi-Qi1,2, ZHANG Xin-You2,*, XU Jing2, ZHANG Zhong-Xin2, LIU Hua2, YAN Mei2, DONG Wen-Zhao2, HUANG Bing-Yan2, HAN Suo-Yi2, TANG Feng-Shou2, LIU Zhi-Yong1,* 1 College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China
2 Industrial Crops Research Institute, Henan Academy of Agricultural Sciences / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry of Agriculture / Henan Provincial Key Laboratory for Genetic Improvement of Oil Crops, Zhengzhou 450002, China
Fund:This study was supported by the Key Project of Science and Technology of Henan Province (141100110600), the China Agriculture Research System of Peanut (CARS-14), the Agriculture Research System of Henan Province (S2012-5), and the Special Fund for Independent Innovation of Henan Academy of Agricultural Science AbstractThe DNA fingerprints of 90 peanut varieties released in Henan province before 2015 were generated using 95 polymorphic loci of 14 SSR markers. All varieties were distinguished by the DNA polymorphisms except for three pairs of varieties with only one SSR locus difference between each pair. SSR clustering analysis revealed that the 90 peanut varieties could be classified into 88 types at a 0.98 genetic similarity coefficient. The remaining two variety pairs had only one allele difference since one variety was the maternal parent of the other. About three fourth (74.4%) of the peanut varieties were distinctive from other varieties at a genetic similarity coefficient threshold of 0.95, indicating a very relatively narrow genetic background of the released peanut varieties in Henan province compared to other crops. Population structure analysis based on 60 SSR markers demonstrated that the 90 varieties could be divided into three subgroups in accordance with the classification according to botanical and pod characteristics, which was consistent with the results of SSR markers cluster analysis. This study provides some fundamental information for selection of parental lines, identification and evaluation of new varieties, as well as establishment of DNA fingerprinting standard in peanut breeding program.
PaikRo OG, Smith RL, Kochert DA. Restriction fragment length polymorphism evaluation of six peanut species within the Arachis section. , 1992, 84: 201-208[本文引用:2]
[2]
王庆贵. DNA指纹图谱在农作物品种鉴定中的应用. 中国标准化, 2001, (7): 45-47Wang QG. The application of DNA fingerprint in the identification of crop variety. China Stand ard, 2001, (7): 45-47 (in Chinese)[本文引用:1]
[3]
翁跃进. 花生AFLP指纹图谱. , 1999, 21(1): 10-12Weng YJ. AFLP fingerprint of peanut. , 1999, 21(1): 10-12 (in Chinese)[本文引用:1]
[4]
李双铃, 任艳, 陶海腾, 禹山林, 袁美. 山东花生主栽品种AFLP指纹图谱的构建. , 2006, 35(1): 18-21Li SL, RenY, Tao HT, Yu SL, YuanM. Establishment of AFLP fingerprinting of peanut cultivars in Shand ong Province. , 2006, 35(1): 18-21 (in Chinese with English abstract)[本文引用:1]
[5]
刘冠明, 郑奕雄, 黎国良. 20个花生品种的SSR标记指纹图谱构建. 中国农学通报, 2006, 22(6): 49-51Liu GM, Zheng YX, Li GL. SSR fingerprinting map of 20 peanut cultivars. Chin Agric Sci Bull, 2006, 22(6): 49-51 (in Chinese with English abstract)[本文引用:1]
[6]
詹世雄, 刘冠明, 郑奕雄, 杨灵, 张平湖, 庄东红. 40个珍珠豆型花生SSR指纹图谱的构建. 种子, 2012, 31(6): 23-27Zhan SX, Liu GM, Zheng YX, YangL, Zhang PH, Zhuang DH. Construction of molecular fingerprinting for forty Spanish- type peanut using SSR markers. Seed, 2012, 31(6): 23-27 (in Chinese with English abstract)[本文引用:1]
He GH, Meng RH, NewmanM, Gao GQ, Pittman RN, Prakash CS. Microsatellite as DNA markers in cultivated peanut (Arachis hypogaea L. ). BMC Plant Biol, 2003, 3: 1-6[本文引用:1]
[9]
He GH, Meng RH, GaoH, Guo BZ, Gao GQ, NewmanM, Pittman RN, Prakash CS. Simple sequence repeat markers for botanical varieties of cultivated peanut (Arachis hypogaea L. ). Euphytica, 2005, 142: 131-136[本文引用:1]
[10]
Ferguson ME, Burow MD, Schulze SR, Bramel PJ, Paterson AH, KresovichS, MitchellS. Microsatellite identification and characterization in peanut (Arachis hypogaea L. ). Theor Appl Genet, 2004, 108: 1064-1070[本文引用:1]
[11]
LiuK, MuseS, PowerMarker: New Genetic Data Analysis Software, Version 2. 7, 2004. http://www.powermarker.net/[本文引用:1]
[12]
DixitA, Jin MH, Chung JW, Yu JW, Chung HK, Ma KH, Park YJ, Cho EG. Development of polymorphic microsatellite markers in sesame (Sesamum indicum L. ). Mol Ecol Notes, 2005, 5: 736-738[本文引用:1]
[13]
Rohlf FJ. NTSYS-pc numerical taxonomy and multivariateanalysis system. Appl. Biostatics, Inc. , New York, , 2000[本文引用:2]
[14]
Sokal RR, Sneath P H A. Principles of Numerical Taxonomy. W, H. Freeman, , 1963[本文引用:2]
[15]
Sneath P HA, Sokal RR. Numerical Taxonomy. Freeman, , 1973. [本文引用:1]
[16]
Sokal RR, Michener CD. A statistical method for evaluating systematic relationships. , 1958, 28: 1409-1438[本文引用:1]
[17]
Pritchard JK, StephensM, DonnellyR. Inference of population structure using multilocus genotype data. , 2000, 155: 945-959[本文引用:1]
[18]
FalushD, StephensM, Pritchard JK. Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. , 2003, 164: 1567-1587[本文引用:1]
[19]
Troyer AF, Rocheford TR. Germplasm ownership: related corn inbreeds. Crop Sci, 2002, 42: 3-10[本文引用:2]
[20]
陆光远, 伍晓明, 张冬晓, 刘凤兰, 陈碧云, 高桂珍, 许鲲. SSR标记分析国家油菜区试品种的特异性和一致性. 中国农业科学, 2008, 41: 32-42Lu GY, Wu XM, Zhang DX, Liu FL, Chen BY, Gao GZ, XuK. SSR-based evaluation of distinctness and uniformity of rapeseed (Brassica napus L. ) varieties under Chinese National Official Field Tests. Sci Agric Sin, 2008, 41: 32-42 (in Chinese with English abstract)[本文引用:3]
[21]
刘红艳, 吴坤, 杨敏敏, 左阳, 赵应忠. 国家芝麻区域试验新品种(系)的DNA指纹分析. 作物学报, 2012, 38: 596-605Liu HY, WuK, Yang MM, ZuoY, Zhao YZ. DNA fingerprinting of sesame (Sesamum indicum L. ) varieties (lines) from recent National Regional Trials in China. Acta Agron Sin, 2012, 38: 596-605 (in Chinese with English abstract)[本文引用:2]