关键词:甘蔗; CAX1基因; 电子克隆; 生物信息学; 实时荧光定量PCR Cloning and Expression Analysis of a Ca2+/H+ Antiporter Gene from Sugarcane SU Wei-Hua**, LIU Feng**, HUANG Long, SU Ya-Chun, HUANG Ning, LING Hui, WU Qi-Bin, ZHANG Hua, QUE You-Xiong* Key Laboratory of Sugarcane Biology and Genetic Breeding (Fujian), Ministry of Agriculture, Fujian Agriculture and Forestry University / Sugarcane Research & Development Center, China Agricultural Technology System, Fuzhou 350002, China Fund:This study was supported by the China Agriculture Research System (CARS-20), the Special Fund for Agro-Scientific Research in the Public Interest (201503119), and the Program for New Century Excellent Talents in Fujian Province University (JA14095) AbstractCAX (Ca2+/H+ antiporter) is a major category of Ca2+ active transport systems in plant cell membrane. In the present study, using a CAX1 mRNA sequence from Sorghum bicolor (GenBank accession number: XM_002441593) as the probe, the full-length cDNA sequence of sugarcane CAX1 gene was cloned by in silico cloning combined with RT-PCR amplification, and named as ScCAX1(GenBank accession number: KT799799). Bioinformatics analysis showed that ScCAX1 has a length of 784 bp and contains a complete open reading frame with a length of 645 bp, which encodes a 214 amino acid residues of sugarcane CAX1 protein. The ScCAX1 protein with stable acidity and hydrophobia was detected to be located in thylakoid membrane of chloroplasts with no signal peptide. It belongs to a conserved Na_Ca_ex. The mainly secondary structure element of ScCAX1 protein is alpha helix. Real time quantitative PCR (RT-qPCR) analysis revealed that the expression of ScCAX1 was tissue-specific, with constituent expression in different tissues of sugarcane. The highest expression was observed in leaf while the lowest in stem. Besides, the expression of ScCAX1gene could be regulated by treatments of PEG, NaCl, SA, ABA, and MeJA. The expression level of this gene was up-regulated by ABA, SA and PEG, with the highest inducible expression level in treatment of 24 hours. The expression level was 5.47 times higher than that of control under 24 hours stress of SA, and 3.5 times higher than that of control under 24 hours stress of ABA. Under 6 hours stress of NaCl, the gene had the highest inducible expression level, which was 2.14 times higher than that of control. This study suggested that ScCAX1 could response to stresses, and its expression may be associated with salt resistance and osmotic tolerance in sugarcane.
Keyword:Sugarcane; CAX1 gene; in silico cloning; Bioinformatics; Real-time quantitative PCR Show Figures Show Figures
图2 同源克隆获得的甘蔗ScCAX1基因的cDNA序列及其推导的氨基酸序列(* 终止密码子) 黑色框部分为特异性引物在基因序列中的位置。Fig. 2 Nucleotide sequence and deduced amino acid sequence of sugarcane ScCAX1 gene obtained by homology cloning (* stop codon) The sequence fragment complementary to primer is highlighted in black box.
图8 甘蔗ScCAX1基因在不同组织中的表达误差线为每组处理的标准误差(n = 3)。Fig. 8 Relative expression of ScCAX1 gene in different tissues of sugarcaneError bars represent the standard error of each treating group (n = 3).
图9 甘蔗 ScCAX1基因在不同外源胁迫下的表达 误差线为每组处理的标准误差(n = 3)。Fig. 9ScCAX1gene expression in sugarcane under different exogenous stresses Error bars represent the standard error of each treating group (n = 3).
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